LOCUS       MR222193                8724 bp    DNA     circular  SYN 
DEFINITION  Dctn1 (Myc-DDK-tagged) - Mouse dynactin 1 (Dctn1), transcript variant 1
ACCESSION   NM_007835
VERSION     NM_007835.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4881..4972 
                     /label="Myc-DDK"  
     misc_feature    5002..5587 
                     /label="PolyA signal"  
     misc_feature    5059..5078 
                     /label="Restriction Enzyme"  
     misc_feature    5863..6535 
                     /label="ColE1"  
     misc_feature    6965..7759 
                     /label="Kan/Neo"  
     misc_feature    7794..8096 
                     /label="SV40 ori"  
     misc_feature    8182..8659 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4863..4869 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4863 
                     /label="Dctn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGAGC AGGAGGCACA TGTCCAGTCG GACGCCGAGT GGCAGCAGGA 
     1081 TGAGTACGGA GGCAAGCGCC CGGCCCCTGC GGGTTGGCTC CCGCGTGGAG GTGATTGGGA 
     1141 AGGGCCACCG AGGCACTGTG GCCTATGTTG GAGCCACACT CTTTGCCACT GGCAAATGGG 
     1201 TGGGCGTGAT TCTGGATGAA GCAAAAGGCA AAAATGATGG CACTGTCCAG GGAAGGAAGT 
     1261 ATTTCACATG TGATGAAGGC CACGGCATCT TTGTACGCCA GTCCCAGATC CAAGTATTTG 
     1321 AAGATGGAGC AGATACTACT TCCCCAGAGA CTCCTGATTC TTCTGCTTCA AAGGTCCTCA 
     1381 AGAGAGAGGG AGCCGATGCA GCTGCAAAGA CCAGCAAACT GCGGGGACTG AAGCCTAAGA 
     1441 AGGCACCGAC AGCCCGAAAG ACCACAACTC GACGGCCCAA GCCTACTCGC CCAGCCAGCA 
     1501 CTGGGGTGGC TGGGCCCAGT AGCTCCCTTG GCCCCTCTGG CTCAGCGTCA GCCGGGGAAC 
     1561 TAAGCAGCAG TGAGCCCAGC ACCCCAGCTC AGACTCCGCT GGCAGCACCC ATCATCCCCA 
     1621 CACCGGCCCT CACCTCTCCT GGAGCAGCAC CCCCACTTCC ATCTCCCTCT AAGGAAGAGG 
     1681 AAGGGCTGAG GGCTCAGGTA CGGGACCTGG AGGAGAAGCT GGAGACCCTG CGCCTAAAAC 
     1741 GCTCAGAAGA CAAAGCAAAG CTGAAAGAGC TGGAGAAGCA CAAGATCCAG CTGGAGCAGG 
     1801 TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCAGA CCTGCAGCGG CGCCTCAAGG 
     1861 AGGCTCGGAA GGAAGCCAAG GAGGCGCTAG AGGCAAAGGA ACGCTACATG GAGGAGATGG 
     1921 CCGACACAGC CGACGCTATC GAGATGGCCA CTCTGGACAA GGAGATGGCT GAAGAGCGCG 
     1981 CTGAGTCTCT GCAGCAAGAG GTGGAGGCAC TGAAGGAACG GGTAGACGAG CTCACCACAG 
     2041 ACCTGGAGAT TCTCAAGGCT GAAATCGAAG AGAAAGGCTC TGATGGGGCC GCATCAAGCT 
     2101 ACCAGCTCAA GCAGCTGGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG GTGAGGATGC 
     2161 GAGACCTCTC TTCCTCAGAG AAGCAGGAGC ACGTGAAGCT GCAGAAACTC ATGGAAAAGA 
     2221 AAAACCAGGA GCTGGAGGTC GTGCGGCAGC AGCGCGAGCG TCTTCAGGAG GAGCTGAGCC 
     2281 AGGCTGAGAG CACCATCGAT GAGCTCAAAG AGCAGGTGGA CGCCGCTCTG GGAGCCGAGG 
     2341 AGATGGTGGA GATGCTGACC GACCGGAACC TGAATCTAGA GGAGAAAGTG CGGGAGTTAC 
     2401 GGGAGACTGT GGGGGACTTG GAAGCCATGA ACGAGATGAA CGATGAGCTG CAGGAGAACG 
     2461 CACGGGAGAC GGAGCTGGAA CTCCGAGAGC AGCTGGACAT GGCGGGCGCC CGAGTGAGGG 
     2521 AAGCGCAGAA GCGAGTGGAA GCCGCCCAGG AGACAGTCGC CGACTACCAG CAGACCATCA 
     2581 AGAAGTACCG CCAGTTGACT GCCCACCTAC AGGATGTCAA TCGGGAGCTG ACAAACCAGC 
     2641 AGGAAGCGTC TGTAGAGAGG CAGCAGCAGC CGCCGCCAGA GACTTTTGAT TTCAAAATCA 
     2701 AGTTTGCTGA GACCAAGGCT CATGCCAAGG CCATTGAGAT GGAGTTGAGA CAGATGGAAG 
     2761 TTGCCCAGGC CAACCGGCAC ATGTCCCTGC TGACAGCCTT TATGCCTGAC AGCTTCCTTC 
     2821 GGCCAGGTGG AGACCACGAC TGTGTCCTGG TGCTGCTGCT CATGCCCCGA CTCATTTGCA 
     2881 AGGCAGAGCT CATCCGGAAG CAGGCCCAGG AGAAGTTTGA CCTGAGCGAG AACTGTTCGG 
     2941 AGCGGCCCGG GCTGCGGGGA GCTGCCGGGG AGCAGCTGAG CTTTGCTGCT GGACTGGTGT 
     3001 ACTCGCTGAG TCTGCTGCAG GCCACGCTGC ACCGCTATGA GCATGCCCTC TCTCAGTGCA 
     3061 GTGTGGACGT GTATAAGAAG GTCGGCAGCC TGTACCCCGA GATGAGCGCC CACGAGCGCT 
     3121 CCTTAGATTT CCTCATTGAG CTGCTGCACA AGGATCAGCT GGATGAGACT GTCAACGTGG 
     3181 AGCCCCTCAC CAAGGCCATC AAGTATTACC AGCATCTGTA CAGCATCCAC CTCGCTGAAC 
     3241 AACCCGAGGA CTCCACCATG CAGCTGGCTG ACCACATCAA GTTCACCCAG AGTGCCCTGG 
     3301 ACTGCATGGG CGTGGAGGTG GGGCGGCTGC GTGCCTTCTT GCAGGGTGGG CAGGAGGCAA 
     3361 CAGATATTGC CCTTCTTCTC CGAGACCTGG AAACATCATG TAGTGACACC CGTCAGTTCT 
     3421 GCAAGAAGAT CCGAAGGCGG ATGCCGGGGA CGGATGCTCC TGGGATCCCA GCAGCGCTGG 
     3481 CCTTTGGCTC ACAGGTATCC GACACACTCC TGGACTGCAG GAAGCACTTG ACGTGGGTGG 
     3541 TAGCTGTTCT GCAGGAGGTG GCAGCTGCAG CCGCCCAGCT TATTGCCCCC TTGGCAGAGA 
     3601 ACGAGGGGCT GCCTGTGGCT GCACTGGAGG AGCTGGCCTT CAAAGCAAGC GAGCAGATCT 
     3661 ACGGGAGCCC CTCCAGCAGC CCCTATGAGT GTCTACGCCA GTCATGCACC ATCCTCATCA 
     3721 GCACGATGAA CAAGCTGGCC ACAGCCATGC AAGAAGGCGA GTATGACGCA GAGCGACCCC 
     3781 CGAGCAAGCC TCCTCCGGTT GAACTTCGGG CTGCAGCCCT GCGTGCAGAG ATCACAGATG 
     3841 CTGAAGGTCT GGGTTTGAAG CTTGAGGATC GAGAGACAGT TATCAAGGAG TTAAAGAAGT 
     3901 CACTCAAGAT TAAGGGAGAG GAGCTGAGTG AGGCCAACGT GCGGCTCAGC CTCCTGGAGA 
     3961 AGAAGTTGGA CAGCGCTGCC AAGGATGCAG ACGAGCGAAT CGAGAAAGTT CAGACACGGC 
     4021 TGGACGAGAC TCAGACCCTG CTGCGGAAGA AGGAGAAAGA CTTTGAGGAG ACAATGGACG 
     4081 CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAGCTCAAG CAGCGCCTGA 
     4141 ACAGCCAGTC CAAGCGCACA ATCGAGGGGC TCCGGGGCCC CCCTCCGTCA GGCATCGCTA 
     4201 CCCTGGTCTC TGGCATCGCT GGTGAGGAAC CACAGCGAGG GGGCGCTCCT GGGCAGGCTC 
     4261 CGGGCGCCTT GCCAGGCCCG GGGCTGGTGA AGGACTCCCC ACTGCTGCTT CAGCAGATCT 
     4321 CTGCTATGAG GCTACACATC TCTCAGCTCC AGCATGAGAA CAGCATCCTC AGAGGAGCCC 
     4381 AGATGAAGGC GTCCTTGGCA GCTCTGCCCC CTCTGCATGT TGCAAAGCTT TCCCTCCCAC 
     4441 CCCATGAGGG CCCCGGTGGT AACCTAGTGG CTGGGGCACT GTACCGCAAG ACCAGCCAGC 
     4501 TCCTGGAGAA ACTAAACCAG CTGAGTACCC ACACCCACGT GGTGGATATC ACTCGGAGCA 
     4561 GCCCAGCTGC CAAGAGCCCG TCAGCTCAGC TTATGGAACA AGTGGCTCAG CTCAAGTCCC 
     4621 TGAGTGACAC CATTGAGAAG CTCAAGGATG AGGTCCTCAA GGAGACAGTG ACTCAGCGCC 
     4681 CTGGAGCCAC TGTGCCCACC GACTTTGCCA CTTTCCCTTC ATCTGCCTTC CTCAGGGCCA 
     4741 AGGAAGAGCA GCAAGATGAC ACAGTCTACA TGGGCAAAGT GACCTTTTCA TGCGCGGCAG 
     4801 GCCTAGGACA GCGACACCGC CTGGTGCTGA CCCAGGAGCA GCTGCACCAG CTTCACAGTC 
     4861 GCCTCATCTC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4921 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4981 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5041 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5101 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5161 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5221 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5281 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5341 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5401 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5461 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5521 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5581 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5641 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5701 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5761 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5821 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5881 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5941 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6001 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6061 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6121 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6181 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6241 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6301 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6361 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6421 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6481 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6541 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6601 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6661 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6721 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6781 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6841 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6901 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6961 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7021 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7081 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7141 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7201 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7261 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7321 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7381 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7441 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7501 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7561 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7621 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7681 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7741 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7801 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7861 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7921 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8161 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8221 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8281 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8341 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8401 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8461 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8521 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8581 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8641 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8701 TAACAAAAAT TTAACGCGAA TTTT
//