LOCUS       MR222188                7614 bp    DNA     circular  SYN 
DEFINITION  Ern2 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2)
ACCESSION   NM_012016
VERSION     NM_012016.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3771..3862 
                     /label="Myc-DDK"  
     misc_feature    3892..4477 
                     /label="PolyA signal"  
     misc_feature    3949..3968 
                     /label="Restriction Enzyme"  
     misc_feature    4753..5425 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Kan/Neo"  
     misc_feature    6684..6986 
                     /label="SV40 ori"  
     misc_feature    7072..7549 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="Ern2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGACCA GTCCAGAGAT TTCAACTGTG GTCTCCTCTC GGGTTCCTGC 
     1081 TGCAGCTTGT GACGTTGCTG GGGAAACTCG GTCCACAGGT CCAGTCTGTC AGGCCAGAGA 
     1141 GCCTTCTGTT TGTGTCTACC TTGGACGGAA GTCTCCATGC ACTGAACAAG CAGACAGGGG 
     1201 ACTTGAAGTG GACAGTGAAG GATGACCCCA TCATCCAGGG CCCAATGTAT GTCACAGAAA 
     1261 TGGCCTTTCT CTCTGACCCA GCTGACGGCA GCCTGTATGT CTTGGGAACC CAGAAACAAC 
     1321 AGGGGCTGAT GAAACTGCCA TTCACCATCC CAGAGCTGGT TCATGCCTCT CCCTGTCGCA 
     1381 GCTCTGATGG TGTCTTCTAC ACAGGCCGGA AGCAGGATGC CTGGTTTGTA GTGGACCCTG 
     1441 AGTCAGGGGA GACTCAGATG ACACTGACCA CAGAAGGCCT CTCTACTCCT CAACTTTTCA 
     1501 TTGGCCGAAC ACAGTACACA GTCTCCATGC ATGACCTACG GACCCCAGCC CTGCGGTGGA 
     1561 ACACAACCTA TCGCCGCTAC TCAGCACCCC TCCTGAATGG CTCACCCGGG AAATACATGA 
     1621 GCCACCTGAC ATCCTGTGGG ATGGGCCTGC TTCTCACTGT GGATCCAGGA AGTGGAATTG 
     1681 TGCTGTGGAC ACAGGACCTG GGAGTGCCTG TGACAGGGAT TTACACATGG CACCAGGATG 
     1741 GTCTGCACCA GCTGCCCCAT CTCACACTGG CTCGGGACAC CCTACATTTC CTTGTCCTCC 
     1801 GCTGGGGCCA CATCCGTCTT CCTGCCTCCA GCTACCAAGA CACAGCCACC CAGTTCTCTT 
     1861 CCTTAGACAC CCAGCTTTTG ATGACACTGT ATGTGGGGAA AGAAGAAGCT GGGTTCTATG 
     1921 TCTCTAAAGC ACTGGTCCAC GCTGGGGTGG CCCTCGTGCC CCGTGGACTG ACCCTGGCAC 
     1981 CCATGGACGG TCCCACAACA GATGAGGTGA CACTTCAAGT CTCGGGGGAG CGAGAGGGCT 
     2041 CACCCAGCAC TGCTGTCAGA TACCCCTCAG GCAGCGTGGC CCTCCCCAGC CAGTGGCTAC 
     2101 TCATTGGATA CCACGAACCT CCCCCCGTTC TACACACAAC CATGCTGCGG GTTCATCCCA 
     2161 TCCCAGGGAA AGTGTCTGCT GAGACACGAG CCTCAGAGGA CCTCCATGCT CCACCTGTCT 
     2221 TTTTTGAGCT CCTAAACCTG AGGAGGGAGG ATCCAGAACT ACATCCTGAA GAGAAAGCTT 
     2281 CAGACTCTTA TCCAGGGCTG GGATCCCAAG ACCTGCTAGC AGCTACCTTC ACTGCCATCC 
     2341 TCCTGGGAGC CTGGGTCCTC TACCTGATGA GGCAGCAGCA GCAGAGCCCT TCAGCACCTG 
     2401 CTGGCCCTCC TGACCTCTCA CAGGATGCTC AGGGACAGCT CTCAAGGGAC ATCTTACAGG 
     2461 ACCAGAGGAG ATTTCAAAGC CCCTCAGAGC CAGCCCAGCC ACCTCATGAC CCTGAAGCAG 
     2521 GGCAGCCCAC CGTGGTGGGG AAGATTTCCT TCAACCCCAA GGATGTGCTG GGCCGTGGGG 
     2581 CAGGAGGGAC TTTTGTTTTC CGAGGACAGT TTGAGGGGCG GGCAGTAGCT GTGAAGAGGC 
     2641 TCCTCCGAGA GTGCTTTGGC TTGGTTCGGC GAGAGGTGCA GCTGCTGCAA GAGTCTGACC 
     2701 GGCACCCCAA CGTGCTTCGC TACTTCTGCA CTGAGCATGG TCCCCAGTTC CACTACATTG 
     2761 CCTTGGAGCT CTGCCAGGCC TCCTTGCAAG AGTACGTGGA GAGCCCAGAC CTAGATCGCT 
     2821 GGGGCCTGGA GCCCACCACA GTGCTGCAGC AGATGATGTC CGGCCTGGCC CATCTACATT 
     2881 CCTTACACAT AGTACACCGT GACCTAAAGC CAGCCAACAT TCTCATGGCT GGGCCTGACA 
     2941 GCCAGGGCCA AGGCAGAGTG GTCATCTCTG ACTTCGGCCT CTGCAAGAAG CTACCTGTTG 
     3001 GCCGTTGTAG CTTCAGTCTC CATTCTGGTA TTCCCGGCAC AGAAGGCTGG ATGGCACCAG 
     3061 AGCTCCTGCA GCTCCCCCCA GACAGCCCGA CCAGCGCTGT GGATATCTTC TCCGCAGGCT 
     3121 GCGTATTTTA TTACGTGCTT TCCGGTGGCA GCCACCCCTT TGGAGAGAGT CTCTACCGAC 
     3181 AGGCCAACAT CCTCTCAGGG GATCCCTGTC TGGCTCAGCT GCAGGAAGAG ACCCATGACA 
     3241 AGGTTGTGGC ATTGGACTTA GTGAGAGCCA TGCTGAGCTT GCTGCCCCAG GATCGCCCCT 
     3301 CGGCAGGATG GGTACTGGCT CACCCCCTCT TTTGGAGCAG AGCTAAGGAG CTCCAGTTCT 
     3361 TCCAGGATGT CAGTGACTGG TTGGAGAAGG AACCAGATCA GGGGCCTCTC GTGTCGGCGC 
     3421 TGGAGGCAGG AAGCTACAAG GTGGTCCGGG AAGACTGGCA CAAGCACATC TCAGCCCCTC 
     3481 TGCAGGCAGA TCTGAAAAGG TTCCGCTCAT ACAAAGGGAC ATCAGTTCGA GACCTGCTCC 
     3541 GGGCCATGAG GAACAAGAAG CACCACTACA GGGAGCTCCC AGCTGAGGTG CGCCAGACAC 
     3601 TAGGCCAACT TCCTGCTGGC TTCATTCAGT ACTTCACACA GCGATTCCCC AGGCTGTTGC 
     3661 TGCACACTCA CCGTGCCATG AGGACCTGCG CCTCTGAGAG CCTCTTCCTG CCCTACTACC 
     3721 CACCAGCTCT GGAAGCCAGG AGGCCAGATG CCACAAAGAG CACGCGTACG CGGCCGCTCG 
     3781 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3841 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3901 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3961 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4021 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4081 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4141 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4201 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4261 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4321 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4381 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4441 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4501 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4561 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4621 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5341 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5401 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5461 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5521 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5581 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5641 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5701 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5761 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5821 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5881 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5941 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6001 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6061 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6121 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6181 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6241 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6301 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6361 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6421 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6481 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6541 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6601 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6661 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6721 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6781 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6841 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7021 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7081 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7141 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7201 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7261 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7321 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7381 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7441 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7501 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7561 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//