LOCUS       MR222176                8349 bp    DNA     circular  SYN 
DEFINITION  Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh)
ACCESSION   NM_001081336
VERSION     NM_001081336.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4506..4597 
                     /label="Myc-DDK"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    4684..4703 
                     /label="Restriction Enzyme"  
     misc_feature    5488..6160 
                     /label="ColE1"  
     misc_feature    6590..7384 
                     /label="Kan/Neo"  
     misc_feature    7419..7721 
                     /label="SV40 ori"  
     misc_feature    7807..8284 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4488..4494 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4488 
                     /label="Dgkh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGGGCC GGCAGCCAGC ACCACCCTCA GGGCGTCGCG GGAGGAGCGG 
     1081 TCGCTGGGGC CAGCGCGGTT TCCCCCACTG CTGCAGGACC GGGAGAGGAT TCGTCCGACA 
     1141 GTGAAGCGGA GCAGGAGGGG CCCCAGAAGC TGATCCGTAA AGTGTCCACC TCTGGACAGA 
     1201 TGCGGACCAA GACTAGTATT AAGGAAGGAC AACTGCTGAA GCAAACCAGC TCTTTCCAAA 
     1261 GGTGGAAGAA GAGATACTTC AAGCTTCGCG GCCGCACACT TTATTATGCG AAAGACTCCA 
     1321 AGTCCTTGAT ATTTGATGAA GTTGACCTCT CAGATGCCAG TGTTGCGGAA GCAAGCACAA 
     1381 AAAATGCCAA CAACAGCTTT ACGATCATCA CTCCGTTCAG AAGACTGATG CTATGTGCTG 
     1441 AGAACAGGAA AGAGATGGAG GTCTGGATCA GCTCTCTGAA GTCAGTGCAG AGCAGAGAAC 
     1501 CCTATGAGGT GGCCCAGTTC AATGTGGAAC ATTTCTCAGG GATGCACAAC TGGTACGCCT 
     1561 GCTCCCACGC CCGGCCCACC TTCTGTAACG TGTGCAGAGA GAGTCTCTCT GGAGTGACCT 
     1621 CCCACGGCCT GTCCTGTGAA GTGTGTAAGT TCAAGGCTCA TAAAAGATGT GCAGTGAGGG 
     1681 CAACAAACAA CTGCAAATGG ACCACGCTGG CCTCCATCGG GAAGGACATC ATAGAGGACG 
     1741 AGGATGGTGT TGCCATGCCT CACCAGTGGC TCGAAGGCAA CTTGCCTGTG AGTGCCAAGT 
     1801 GTGCAGTCTG TGACAAAACC TGTGGCAGTG TGCTTCGCCT GCAAGATTGG AAATGCCTTT 
     1861 GGTGTAAGAC AATGGTGCAC ACTGCCTGCA AAGATGTGTA CCATCCCGTC TGTCCCCTTG 
     1921 GCCAGTGTAA AGTCTCCATC ATACCTCCGA TTGCACTAAA TAGCACAGAC TCTGATGGTT 
     1981 TCTGTCGAGC AACCTTTTCG TTCTGTGTCA GCCCCCTCTT GGTGTTCGTC AACTCTAAGA 
     2041 GTGGAGATAA TCAGGGAGTG AAGTTCCTTC GTCGCTTTAA ACAGTTGCTC AACCCAGCTC 
     2101 AGGTGTTTGA TTTAATGAAC GGAGGGCCTT ATTTAGGCTT GAGATTGTTT CAAAAGTTTG 
     2161 ACAACTTTCG GATCCTTGTC TGTGGAGGAG ATGGAAGTGT AGGCTGGGTT TTGTCAGAGA 
     2221 TTGATAAACT GAACTTGCAT AAACAGTGCC AGCTTGGGGT GCTACCCTTG GGTACCGGCA 
     2281 ATGACCTTGC TCGAGTTCTT GGCTGGGGAG GTTCATATGA TGATGACACC CAACTCCCTC 
     2341 AGATACTAGA GAAGCTGGAA CGAGCCAGTA CCAAAATGCT GGACAGGTGG AGTATAATGA 
     2401 CCTATGAGCT CAAATTGCCA GCAAAGTCGT CTCTACTTCC TGAACCTGTG GCAGCAACTG 
     2461 AAGAATTTTA CATGACAATT TATGAAGACT CCGTTGCAAA CCATCTTACG AAAATCGTCA 
     2521 ACTCTGATGA GCATGCGGTG GTCATATCGT CTGCCAAGAT ACTCTGTGAA ACCGTGAAGG 
     2581 ACTTCGTTGC CAAAGTGGAG AAGGCACAGG ACAGAACACT GGAAAATACA GTAGTAGCCG 
     2641 AAGCCGTGGC CAGTAAATGC TCAGTCCTAA ACGAGAAGCT TGAGCAGTTG CTACAAGCCT 
     2701 TGCACGCGGA CTCTCAGGCC AGTCGAGTCC CCCCAGGAAT CGGCCCTGCC ATCCCCGAAG 
     2761 AGGACACCGT GGAGTCCGCC AGTGACGAGT CCTTGGGTGA AAGCAAGGAC CAGCTGGTGA 
     2821 ATGACATTGG GAAACCCTCC TCCCAGAAAG CCGTGAAGCC CCGGGAGATA ATGCTGCGGG 
     2881 CCAACAGTCT AAAGAAAGCG GTGAGGCAAG TCATTGAAGA AGCTGAAAAA GTTATGGATG 
     2941 AGCCAGCAGT GCAGCCCTCT GAGCCAGTCA GTCCGTCTTG TGACTATGAT ACAGAAACAG 
     3001 ATGAAGCTAA AGAGGACGAC GCGAAAGAGT CGCTATCAGC CAAAACCACA TCTCAGTCTC 
     3061 CTGATGCCCA GGCAAGCTGT GGCCATCCCC AAACTGACTC TGTCGCTGGT CCAGCCATGG 
     3121 CCACCACCAA AGAAAACCTC CCTGTGCTCA ACACTAGAAT CATCTGCCCA GGTTTGAGAG 
     3181 CAGGACTTGC TGCCTCCATT GCTGGGAGTT CTATCATCAA CAAAATGTTG CTGGCGAACA 
     3241 TCGATCCTTT CGGGGCAACA CCGTTTATTG ACCCAGATCT AGACTCACTA GATGGATACT 
     3301 CTGAAAAATG TGTCATGAAC AACTACTTTG GGATTGGATT AGATGCAAAA ATTTCATTAG 
     3361 AATTTAATAA TAAGAGAGAG GAGCACCCTG AAAAATGCAG AAGTCGAACT AAAAACTTGA 
     3421 TGTGGTACGG AGTCCTTGGG ACCCGGGAGC TACTACAGAG ATCGTACAAG AATCTGGAGC 
     3481 AGAGGGTTCA ACTTGAGTGT GATGGGCAGT ATATACCTCT TCCTAGTCTA CAAGGCATAG 
     3541 CGGTGTTGAA CATCCCTAGC TACGCCGGAG GCACCAACTT CTGGGGCGGA ACCAAAGAGG 
     3601 ATGATATATT TGCTGCGCCA TCCTTTGATG ACAAGATTCT AGAAGTTGTC GCAGTGTTTG 
     3661 ACAGTGTGCA GATGGCAGTA TCAAGGGTCA TTAAATTGCA GCATCACCGG ATAGCACAGT 
     3721 GCCGTACGGT AAAAATCACG ATATTTGGCG ACGAGGGAGT CCCAGTGCAG GTGGATGGTG 
     3781 AAGCATGGGT CCAGCCTCCA GGGATCATCA AGATTGTGCA CAAAAACAGA GCTCAGATGC 
     3841 TAACCAGAGA CAGAGCCTTT GAAAGCACCC TGAAGTCTTG GGAAGACAAG CAGAAGTGTG 
     3901 ACTCTGGGAA GCCAGTTCTT CGAACCAACC TGTACATCCA TCCCGCCCCT GACCTGGCAA 
     3961 CAGAGGAAGT GTCACAGATG CGGCTCTGCT CCCAGGCTGC GGAGGAACTC ATCACTAGGA 
     4021 TTTGTGACGC AGCCACGATT CACTGTCTCC TGGAGCAGGA GCTGGCACAT GCAGTGAATG 
     4081 CCTGCTCACA CGCCCTGAAC AAAGCCAACC CACGCTTCCC GGAGAGCCTT ACGAGAGACA 
     4141 CTGCCACCGA GATAGCCATC AACGTCAAGG CGCTCTATAA TGAGACAGAG GCTTTGCTAG 
     4201 TTGGCAGGGT CCCTTTGCAC TTGGAATCTC CTCACGAAGA GCGGGTGTCC AGTGCCTTAC 
     4261 ACTCTGTGGA GATGGAGCTT CAGAAGTTAA CGGAGATTCC CTGGCTTTAT TACATCCTCC 
     4321 GCCCCAGTGA GGACGAGGAG CCTCCTCTGG ATTGTACCAA AAGAAACAAC AAAAGCACAG 
     4381 TCTTTCGCAT AGTGCCAAAG TTTAAAAAGG AAAAGGCTCA GAAACAGAAA ACAAGTTCGC 
     4441 AGCCTGGACC TGGGGATTCC GAAAGTGGGT CATATGAAGC GAATTCTCCA GGGAATACGC 
     4501 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4561 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5281 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5341 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5461 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5521 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5581 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5641 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5761 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5821 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5881 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5941 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6061 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6121 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6181 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6241 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6301 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6361 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6421 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6481 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6541 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6601 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6661 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6721 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6781 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6841 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6901 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6961 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7021 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7081 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7141 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7201 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7261 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7321 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7381 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7441 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7501 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7561 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7921 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7981 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8041 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8101 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8161 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8221 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8281 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8341 GCGAATTTT
//