LOCUS       MR222168                7418 bp    DNA     circular  SYN 
DEFINITION  Nlgn2 (Myc-DDK-tagged) - Mouse neuroligin 2 (Nlgn2)
ACCESSION   NM_198862
VERSION     NM_198862.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3576..3667 
                     /label="Myc-DDK"  
     misc_feature    3697..4282 
                     /label="PolyA signal"  
     misc_feature    3754..3773 
                     /label="Restriction Enzyme"  
     misc_feature    4558..5230 
                     /label="ColE1"  
     misc_feature    5660..6454 
                     /label="Kan/Neo"  
     misc_feature    6489..6791 
                     /label="SV40 ori"  
     misc_feature    6877..7354 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3535..3541 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3535 
                     /label="Nlgn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCATG TGGCTCCTGG CGTTGTGTCT GGTGGGGCTG GCTGGGGCTC AACGGGGAGG 
     1081 AGGGGGTCCC GGCGGCGGCG CCCCGGGCGG CCCAGGCCTG GGCCTCGGCA GCCTCGGGGA 
     1141 GGAGCGCTTC CCCGTGGTGA ACACAGCCTA CGGGCGAGTG CGCGGTGTGC GGCGCGAGCT 
     1201 CAACAACGAG ATCCTGGGCC CGGTCGTGCA GTTCTTGGGC GTGCCCTACG CCACGCCGCC 
     1261 CTTGGGCGCC CGCCGCTTCC AGCCGCCTGA GGCACCTGCC TCGTGGCCCG GCGTGCGCAA 
     1321 CGCCACCACC CTGCCGCCCG CCTGCCCGCA GAACCTGCAC GGGGCCCTGC CGGCCATCAT 
     1381 GCTGCCTGTG TGGTTCACCG ACAACTTGGA GGCGGCCGCC ACCTACGTGC AGAACCAGAG 
     1441 CGAGGACTGC CTGTACCTCA ACCTCTACGT GCCCACTGAG GACGGTCCGC TCACAAAAAA 
     1501 ACGTGACGAG GCGACGCTCA ATCCGCCAGA CACAGATATC CGGGACTCTG GGAAGAAACC 
     1561 GGTCATGCTG TTTCTACACG GCGGCTCCTA CATGGAAGGC ACCGGGAACA TGTTTGACGG 
     1621 CTCAGTCCTG GCTGCCTATG GCAATGTCAT CGTAGTCACA CTCAACTACC GTCTTGGGGT 
     1681 GCTCGGTTTT CTCAGCACTG GTGACCAGGC TGCAAAAGGC AACTATGGGC TCCTGGACCA 
     1741 GATCCAGGCC CTGCGCTGGC TCAGTGAAAA CATTGCCCAC TTTGGAGGTG ACCCTGAACG 
     1801 CATCACTATC TTTGGGTCTG GTGCAGGGGC CTCCTGTGTC AACTTGCTGA TCCTTTCCCA 
     1861 CCACTCAGAA GGACTGTTCC AGAAGGCCAT TGCTCAAAGT GGTACTGCCA TTTCCAGCTG 
     1921 GTCTGTCAAC TACCAGCCGC TCAAGTACAC GCGGCTGCTG GCGGCCAAAG TGGGCTGTGA 
     1981 CCGAGAAGAC AGCACTGAAG CTGTGGAGTG TCTGCGCCGG AAGTCTTCCC GGGAGCTAGT 
     2041 GGACCAGGAT GTACAGCCTG CCCGCTACCA CATTGCCTTT GGGCCTGTGG TGGACGGCGA 
     2101 CGTAGTCCCT GATGACCCCG AGATCCTCAT GCAACAGGGG GAATTCCTCA ACTACGACAT 
     2161 GCTCATTGGT GTCAACCAGG GAGAGGGTCT CAAGTTCGTG GAGGACTCTG CAGAGAGTGA 
     2221 GGACGGTGTG TCTGCCAGCG CCTTTGACTT CACCGTCTCC AACTTTGTGG ACAACTTGTA 
     2281 CGGGTACCCA GAAGGCAAGG ACGTGCTTCG AGAGACCATC AAGTTCATGT ACACGGACTG 
     2341 GGCTGACAGG GACAATGGCG AGATGCGGCG GAAGACCCTG CTGGCGCTCT TTACCGACCA 
     2401 CCAGTGGGTC GCCCCGGCTG TGGCCACCGC CAAGCTGCAT GCCGACTACC AGTCCCCCGT 
     2461 CTACTTTTAC ACTTTCTACC ACCACTGCCA GGCAGAGGGC CGGCCAGAGT GGGCAGACGC 
     2521 AGCGCACGGG GACGAGCTGC CCTACGTCTT TGGTGTCCCC ATGGTGGGCG CCACTGACCT 
     2581 CTTCCCCTGC AACTTCTCCA AGAACGATGT CATGCTCAGC GCAGTAGTCA TGACCTATTG 
     2641 GACCAACTTC GCCAAGACTG GTGACCCCAA CCAGCCTGTG CCACAGGACA CCAAGTTCAT 
     2701 CCACACCAAG CCCAACCGCT TTGAAGAGGT AGTGTGGAGC AAGTTCAACA GCAAGGAAAA 
     2761 GCAGTATCTG CACATAGGCT TGAAACCACG CGTGCGCGAC AACTACCGTG CCAACAAGGT 
     2821 GGCCTTCTGG CTGGAGCTCG TGCCCCACCT GCACAACCTG CACACAGAGC TCTTCACCAC 
     2881 CACCACTCGC CTGCCTCCCT ATGCCACACG CTGGCCACCT CGCACACCTG GTCCTGGCAC 
     2941 TTCCGGCACA CGCCGTCCTC CCCCACCTGC CACTCTGCCA CCTGAGTCTG ATATTGACCT 
     3001 AGGCCCAAGG GCCTATGACC GCTTCCCCGG TGACTCGAGG GACTACTCCA CGGAGCTAAG 
     3061 CGTGACTGTG GCAGTGGGTG CCTCCCTCCT CTTCCTCAAC ATCCTTGCCT TTGCCGCCCT 
     3121 CTATTACAAG CGGGACCGGC GCCAGGAGCT GCGGTGCCGG AGGCTTAGCC CACCAGGAGG 
     3181 CTCAGGCTCA GGTGTGCCTG GTGGGGGCCC CCTGCTTCCC ACTGCTGGCC GTGAGCTACC 
     3241 CCCGGAGGAG GAGCTAGTAT CGCTGCAGCT GAAGCGGGGT GGTGGTGTTG GGGCGGACCC 
     3301 TGCTGAGGCC CTGCGCCCTG CCTGTCCACC CGACTATACC CTGGCCTTGC GCCGGGCACC 
     3361 GGACGATGTG CCTCTCTTGG CCCCCGGGGC CCTAACCCTG CTGCCTAGTG GCCTGGGGCC 
     3421 CCCGCCCCCG CCCCCACCCC CTTCTCTCCA TCCCTTTGGG CCCTTCCCAC CCCCACCCCC 
     3481 TACTGCTACC AGCCACAACA ACACGCTACC CCATCCCCAC TCCACCACTC GGGTACAAGC 
     3541 TTAAGCTTAA CTAGCTAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC 
     3601 AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA 
     3661 AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC 
     3721 CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC 
     3781 TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT 
     3841 GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG 
     3901 TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT 
     3961 CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA 
     4021 TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA 
     4081 GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG 
     4141 GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC 
     4201 AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT 
     4261 TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG 
     4321 AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 
     4381 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 
     4441 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 
     4501 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 
     4561 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 
     4621 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 
     4681 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 
     4741 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 
     4801 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 
     4861 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 
     4921 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 
     4981 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 
     5041 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 
     5101 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 
     5161 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT 
     5221 GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA 
     5281 CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC 
     5341 GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC 
     5401 CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT 
     5461 ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG 
     5521 AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC 
     5581 CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG 
     5641 TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG 
     5701 CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT 
     5761 CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG 
     5821 CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT 
     5881 TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT 
     5941 TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT 
     6001 GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT 
     6061 GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA 
     6121 TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC 
     6181 CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA 
     6241 CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC 
     6301 CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG 
     6361 CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC 
     6421 AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC 
     6481 CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC 
     6541 TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG 
     6601 CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG 
     6661 GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG 
     6721 GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT 
     6781 CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG 
     6841 TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     6901 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     6961 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     7021 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     7081 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     7141 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     7201 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     7261 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     7321 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     7381 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//