LOCUS       MR222149                9381 bp    DNA     circular  SYN 
DEFINITION  Cps1 (Myc-DDK-tagged) - Mouse carbamoyl-phosphate synthetase 1 (Cps1), nuclear gene encoding mitochondrial protein
ACCESSION   NM_001080809
VERSION     NM_001080809.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5538..5629 
                     /label="Myc-DDK"  
     misc_feature    5659..6244 
                     /label="PolyA signal"  
     misc_feature    5716..5735 
                     /label="Restriction Enzyme"  
     misc_feature    6520..7192 
                     /label="ColE1"  
     misc_feature    7622..8416 
                     /label="Kan/Neo"  
     misc_feature    8451..8753 
                     /label="SV40 ori"  
     misc_feature    8839..9316 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5520..5526 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5520 
                     /label="Cps1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAGGATT TTGACAGCTT GCAAAGTGGT GAAGACCCTG AAGAGTGGCT 
     1081 TTGGTTTTGC CAATGTGACT ACGAAGCGAC AGTGGGACTT CTCCAGACCT GGCATCAGGC 
     1141 TCCTTTCTGT GAAGGCAAAG ACAGCACACA TTGTTCTGGA AGATGGAACT AAGATGAAAG 
     1201 GCTACTCCTT TGGCCATCCC TCCTCTGTTG CTGGTGAAGT GGTTTTTAAT ACTGGCTTAG 
     1261 GAGGGTACCC GGAAGCACTT ACTGATCCCG CCTACAAGGG GCAGATCCTC ACCATGGCCA 
     1321 ACCCTATCAT TGGGAACGGT GGGGCTCCCG ACACCACTGC CCGAGACGAA CTGGGACTGA 
     1381 ATAAGTACAT GGAGTCTGAT GGGATCAAGG TGGCAGGTCT GCTGGTTCTG AATTACAGTA 
     1441 ATGACTATAA CCACTGGCTG GCTACCAAGA GTCTGGGTCA ATGGCTGCAG GAGGAAAAGG 
     1501 TCCCTGCAAT TTATGGAGTG GATACAAGAA TGCTGACTAA AATAATTCGG GATAAGGGTA 
     1561 CCATGCTTGG GAAGATTGAA TTTGAAGGCC AGTCTGTGGA TTTTGTGGAT CCCAATAAGC 
     1621 AGAATTTGAT TGCTGAGGTT TCAACCAAGG ATGTCAAGGT GTTTGGCAAA GGAAACCCCA 
     1681 CGAAAGTGGT AGCTGTGGAC TGTGGGATAA AAAACAATGT CATCCGCCTG CTAGTTAAGC 
     1741 GAGGAGCTGA AGTGCATTTG GTTCCCTGGA ATCACGACTT CACCCAGATG GAGTATGATG 
     1801 GACTTTTGAT TGCTGGGGGA CCTGGGAATC CAGCTCTTGC ACAGCCACTA ATTCAGAATG 
     1861 TGAAAAAGAT TTTGGAGAGT GACCGCAAAG AGCCATTGTT TGGAATCAGT ACAGGAAACA 
     1921 TAATAACAGG ATTGGCTGCT GGTGCCAAAT CCTACAAGAT GTCCATGGCC AACAGAGGAC 
     1981 AGAACCAACC TGTTTTGAAT ATCACAAACA GACAGGCTTT CATAACTGCT CAGAATCATG 
     2041 GCTATGCTCT GGACAACACA CTCCCTGCTG GCTGGAAACC ACTGTTTGTG AATGTCAATG 
     2101 ATCAAACAAA CGAGGGGATT ATGCATGAGA GCAAACCTTT CTTCGCTGTG CAGTTCCACC 
     2161 CAGAGGTCAG CCCGGGGCCA ACAGACACTG AGTACCTATT TGATTCCTTC TTCTCCCTGA 
     2221 TAAAGAAGGG CAAAGGCACC ACCATTACCT CAGTTCTGCC CAAGCCAGCG TTGGTGGCAT 
     2281 CTCGTGTCGA GGTTTCCAAG GTCCTCATCC TGGGATCAGG AGGTCTGTCC ATTGGTCAGG 
     2341 CAGGTGAATT TGATTACTCG GGATCTCAAG CTGTGAAAGC CATGAAGGAA GAAAATGTCA 
     2401 AAACAGTCCT GATGAACCCG AACATCGCAT CCGTGCAGAC CAACGAGGTG GGATTGAAGC 
     2461 AGGCAGATGC AGTCTACTTT CTCCCTATTA CCCCCCAGTT TGTCACAGAA GTTATCAAGG 
     2521 CTGAACGGCC TGATGGGCTG ATTCTGGGCA TGGGTGGCCA GACAGCTCTG AACTGTGGAG 
     2581 TGGAACTATT CAAGAGGGGT GTGCTCAAGG AATATGGGGT GAAGGTCTTG GGCACATCAG 
     2641 TTGAGTCCAT TATGGCCACA GAAGACAGGC AGCTGTTCTC AGACAAGCTG AATGAGATCA 
     2701 ACGAGAAGAT CGCACCCAGT TTTGCAGTGG AATCAATGGA GGATGCCTTG AAGGCAGCAG 
     2761 ACACCATTGG CTACCCTGTG ATGATTCGGT CTGCATATGC TCTGGGTGGC TTAGGCTCTG 
     2821 GCATCTGTCC CAATAAGGAG ACCTTAATAG ACCTTGGCAC AAAAGCATTT GCTATGACCA 
     2881 ACCAGATCCT GGTGGAGCGG TCAGTGACAG GTTGGAAAGA AATAGAATAT GAAGTTGTCC 
     2941 GAGATGCAGA TGATAACTGT GTCACAGTCT GTAACATGGA AAATGTTGAC GCCATGGGTG 
     3001 TTCACACAGG TGACTCGGTT GTCGTGGCCC CAGCCCAGAC ACTCTCCAAT GCAGAGTTCC 
     3061 AGATGTTGAG ACGCACTTCA GTCAATGTTG TTCGTCACTT GGGCATTGTG GGCGAATGCA 
     3121 ACATTCAGTT TGCTCTTCAC CCCACTTCCA TGGAATACTG TATCATTGAA GTGAATGCCA 
     3181 GGCTGTCCCG GAGTTCTGCC CTGGCCTCCA AGGCCACTGG CTACCCACTG GCATTCATTG 
     3241 CAGCAAAGAT TGCTCTAGGA ATCCCACTTC CAGAAATCAA GAATGTTGTA TCTGGGAAGA 
     3301 CCTCAGCCTG CTTCGAACCT AGCCTGGATT ACATGGTGAC CAAGATTCCT CGCTGGGATC 
     3361 TTGACCGTTT CCATGGAACA TCCAGCCGAA TTGGTAGCTC AATGAAAAGT GTGGGCGAGG 
     3421 TCATGGCCAT TGGTCGCACC TTTGAGGAAA GTTTCCAGAA GGCTCTGAGG ATGTGCCATC 
     3481 CATCTGTGGA TGGGTTCACT CCCCGGCTCC CAATGAATAA GGAATGGCCA GCAAACCTGG 
     3541 ATCTGAAGAA AGAGCTGTCT GAGCCCTCCA GCACCCGCAT CTACGCCATT GCTAAGGCCT 
     3601 TGGAGAACAA CATGTCCCTC GACGAAATTG TGAGGCTCAC GTCCATCGAC AAGTGGTTTC 
     3661 TGTACAAGAT GCGTGACATT TTAAACATGG ATAAGACACT GAAAGGGCTT AACAGTGACT 
     3721 CTGTTACAGA AGAAACTCTG AGAAAGGCCA AGGAGATTGG GTTCTCAGAC AAGCAGATTT 
     3781 CAAAATGTTT GGGACTGACT GAAGCCCAGA CAAGAGAGCT GAGGTTAAAG AAAAACATCC 
     3841 ACCCTTGGGT TAAACAGATT GATACATTGG CTGCAGAATA CCCATCAGTA ACAAACTACC 
     3901 TGTATGTTAC CTACAACGGC CAGGAGCACG ATATCAAATT TGATGAACAT GGAATAATGG 
     3961 TTCTGGGCTG TGGTCCATAC CACATTGGCA GCAGTGTGGA ATTTGATTGG TGTGCTGTCT 
     4021 CCAGTATCCG TACACTGCGC CAACTTGGCA AGAAGACAGT GGTGGTGAAT TGTAACCCAG 
     4081 AGACTGTGAG CACTGATTTT GATGAGTGTG ACAAACTCTA CTTTGAAGAG CTGTCTTTGG 
     4141 AGAGAATCCT AGACATCTAC CACCAGGAGG CATGTAATGG CTGCATCATA TCAGTTGGCG 
     4201 GCCAGATTCC AAACAACTTG GCGGTCCCGC TGTACAAGAA TGGTGTCAAG ATCATGGGTA 
     4261 CAAGCCCTCT GCAGATCGAC AGGGCTGAGG ATCGCTCCAT CTTCTCAGCT GTCTTAGATG 
     4321 AGCTAAAGGT GGCCCAGGCT CCATGGAAAG CTGTGAACAC TTTGAATGAA GCACTGGAAT 
     4381 TTGCAAACTC TGTGGGCTAC CCCTGCTTAC TGAGACCTTC CTATGTTTTG AGTGGGTCTG 
     4441 CCATGAACGT GGTATTCTCT GAGGATGAGA TGAAAAGGTT TCTTGAGGAG GCCACTCGAG 
     4501 TCTCTCAGGA ACACCCGGTG GTGCTGACCA AGTTTGTGGA GGGGGCCCGG GAAGTAGAGA 
     4561 TGGACGCTGT TGGCAAAGAA GGACGGGTCA TCTCCCATGC CATCTCTGAA CATGTTGAAG 
     4621 ATGCAGGTGT CCACTCAGGA GATGCCACCC TGATGCTACC TACACAGACC ATCAGCCAAG 
     4681 GAGCCATTGA AAAGGTGAAG GATGCCACAC GAAAGATTGC AAAAGCCTTT GCCATCTCTG 
     4741 GGCCATTCAA TGTCCAGTTT CTTGTCAAAG GAAATGATGT CTTGGTGATT GAGTGCAATC 
     4801 TGAGAGCATC AAGATCCTTC CCCTTTGTCT CCAAGACTCT TGGGGTGGAC TTCATTGACG 
     4861 TGGCTACAAA GGTGATGATC GGGGAGAGCA TTGATGAGAA GCGACTTCCC ACGCTGGAGC 
     4921 AGCCCATCAT TCCCTCTGAC TATGTTGCCA TTAAGGCTCC AATGTTTTCC TGGCCCCGAC 
     4981 TGAGAGATGC TGACCCTATT CTGAGATGTG AGATGGCTTC TACTGGAGAG GTGGCGTGCT 
     5041 TCGGCGAGGG CATTCATACC GCCTTCCTAA AGGCCATGCT GTCTACAGGG TTTAAGATAC 
     5101 CTCAGAAGGG CATTCTGATT GGAATCCAGC AATCATTCCG TCCAAGATTC CTTGGTGTTG 
     5161 CTGAGCAGTT GCACAATGAA GGTTTCAAGC TTTTTGCCAC AGAAGCTACA TCAGACTGGC 
     5221 TCAATGCCAA CAATGTCCCT GCCACCCCTG TGGCTTGGCC ATCTCAGGAA GGACAGAATC 
     5281 CCAGCCTCTC TTCCATCAGA AAGTTGATAA GAGATGGAAG CATTGACCTA GTGATTAACC 
     5341 TCCCCAATAA CAACACCAAA TTTGTCCATG ATAATTACGT GATTCGGAGG ACAGCTGTGG 
     5401 ACAGTGGAAT TGCTCTCCTC ACCAATTTCC AGGTGACCAA ACTTTTTGCT GAAGCTGTGC 
     5461 AGAAATCTCG CACTGTGGAC TCCAAGAGTC TGTTCCACTA CAGGCAGTAC AGTGCTGGAA 
     5521 AGGCCGCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5581 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5641 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5701 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5761 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5821 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5881 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5941 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     6001 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     6061 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6121 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6181 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6241 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6301 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6361 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6421 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6481 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6541 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6601 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6661 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6721 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6781 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6841 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6901 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6961 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     7021 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     7081 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7141 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7201 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7261 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7321 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7381 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7441 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7501 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7561 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7621 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7681 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7741 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7801 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7861 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7921 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7981 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     8041 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     8101 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8161 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8221 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8281 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8341 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8401 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8461 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8521 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8581 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8641 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8701 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8761 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8821 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8881 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8941 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     9001 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     9061 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9121 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9181 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9241 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9301 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9361 CAAAAATTTA ACGCGAATTT T
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