LOCUS       MR222147                8970 bp    DNA     circular  SYN 
DEFINITION  Tnik (Myc-DDK-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 4
ACCESSION   NM_001163009
VERSION     NM_001163009.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5127..5218 
                     /label="Myc-DDK"  
     misc_feature    5248..5833 
                     /label="PolyA signal"  
     misc_feature    5305..5324 
                     /label="Restriction Enzyme"  
     misc_feature    6109..6781 
                     /label="ColE1"  
     misc_feature    7211..8005 
                     /label="Kan/Neo"  
     misc_feature    8040..8342 
                     /label="SV40 ori"  
     misc_feature    8428..8905 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5109..5115 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5109 
                     /label="Tnik"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCAGCTC GCAGCCTGGA TGAAATCGAT CTCTCCGCCC 
     1081 TGAGGGACCC TGCAGGGATC TTTGAGTTGG TGGAACTTGT CGGAAATGGC ACGTATGGTC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC TGCCATTAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACAGGAA CATTGCTACA TACTACGGTG CTTTTATCAA AAAGAACCCT CCTGGCATGG 
     1321 ATGACCAACT CTGGTTGGTT ATGGAGTTCT GTGGTGCTGG CTCTGTCACT GACCTGATCA 
     1381 AGAACACGAA AGGCAACACA TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAGATCT 
     1441 TACGGGGCCT GAGTCACCTG CACCAGCACA AAGTGATTCA TCGAGATATC AAAGGGCAGA 
     1501 ACGTCTTGTT GACTGAAAAT GCAGAGGTTA AGCTAGTGGA TTTTGGAGTG AGTGCCCAGC 
     1561 TTGACCGAAC TGTGGGCAGG AGGAACACGT TCATCGGGAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTCATTGC CTGTGATGAG AACCCGGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGAAT CACCGCCATT GAGATGGCAG AAGGTGCCCC CCCCCTCTGT GACATGCATC 
     1741 CCATGAGAGC CCTCTTCCTC ATCCCACGGA ACCCTGCACC TCGGCTCAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATCGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGGC 
     1861 CAGCCACGGA GCAGTTGATG AAGCACCCAT TCATACGAGA CCAACCTAAT GAGAGGCAGG 
     1921 TCCGCATCCA GCTGAAGGAC CACATTGATC GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACTGAGTA TGAATACAGC GGAAGTGAGG AAGAAGAGGA AGAGAATGAC TCTGGGGAAC 
     2041 CCAGCTCCAT TCTGAACCTA CCAGGGGAGT CAACACTGCG AAGGGACTTC CTGAGACTGC 
     2101 AGCTGGCCAA CAAGGAGCGC TCAGAGGCCC TGCGGCGCCA ACAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA AGAACACAAG CGGCAGCTAC TGGCTGAGCG CCAGAAGCGC ATCGAAGAGC 
     2221 AGAAGGAGCA AAGGCGGAGG CTGGAGGAGC AACAAAGGCG AGAAAAAGAG CTTCGGAAAC 
     2281 AGCAGGAGCG GGAACAGCGC CGGCACTACG AAGAACAGAT GCGTCGGGAG GAGGAGAGGA 
     2341 GGCGTGCCGA ACATGAGCAG GAATACATCA GGCGGCAGCT AGAAGAAGAG CAAAGGCAGT 
     2401 TAGAGATCTT ACAGCAGCAG CTACTGCATG AGCAAGCTCT ACTTCTGGAA TATAAGCGCA 
     2461 AACAATTGGA AGAACAGAGA CAAGCAGAAA GACTGCAGAG GCAGCTAAAG CAAGAGCGGG 
     2521 ACTATCTGGT TTCCCTCCAG CATCAGCGGC AGGAGCAGAG GCCCCTGGAG AAGAAGCCAC 
     2581 TGTACCATTA CAAGGAGGGC ATGAGTCCTA GTGAGAAGCC GGCCTGGGCC AAGGAGGTAG 
     2641 AAGAACGCTC AAGACTCAAC CGACAGAGTT CACCTGCCAT GCCTCACAAG GTTGCCAACA 
     2701 GGATCTCGGA CCCCAACCTG CCCCCAAGAT CAGAGTCCTT CAGCATTAGT GGGGTTCAGC 
     2761 CTGCAAGGAC ACCCCCAATG CTCAGACCTG TTGACCCCCA GATCCCGCAG CTGGTAGCTG 
     2821 TCAAATCCCA GGGACCTGCC TTGACGGCCT CCCAGTCAGT ACATGAGCAA CCCACAAAGG 
     2881 GCCTGTCTGG GTTCCAGGAG GCTCTGAATG TGACCTCTCA CCGGGTCGAG ATGCCACGCC 
     2941 AGAACTCGGA TCCCACCTCA GAAAACCCTC CTCTCCCCAC GAGAATTGAG AAGTTTGACA 
     3001 GAAGCTCTTG GTTACGACAG GAAGAAGACA TTCCACCAAA GGTGCCTCAA AGAACAACTT 
     3061 CTATATCCCC AGCACTAGCC AGAAAGAATT CCCCTGGCAA TGGCAGTGCT CTGGGCCCCA 
     3121 GACTTGGATC TCAGCCCATC AGAGCAAGCA ACCCTGATCT GCGCAGGACA GAGCCAGTCC 
     3181 TGGAGAGTTC CCTGCAGCGG ACAAGCAGTG GCAGTTCCTC CAGCTCCAGC ACTCCCAGCT 
     3241 CCCAGCCCAG CTCCCAAGGA GGCTCTCAAC CTGGCTCCCA AGCAGGATCT AGTGAGCGGT 
     3301 CCAGAGTGCG GGCCAACAGT AAGTCCGAAG GATCACCCGT GCTCCCCCAT GAGCCTTCCA 
     3361 AGGTGAAACC AGAAGAATCC AGAGACATCA CACGGCCCAG TCGGCCAGCT GATCTGACGG 
     3421 CATTAGCCAA AGAATTACGA GAACTCCGCA TTGAAGAAAC AAACCGCCCC CTGAAGAAAG 
     3481 TGACTGATTA CTCTTCCTCC AGCGAGGAGT CGGAGAGCAG TGAGGAAGAA GAGGAAGATG 
     3541 GAGAGAGTGA GACACATGAC GGGACGGTGG CTGTCAGTGA CATACCCAGA CTAATACCCA 
     3601 CCGGAGCTCC AGGGAACAAT GAGCAGTACA ACATGGGGAT GGTCGGGACA CATGGGCTGG 
     3661 AAACTTCGCA TGCGGACACC TTTGGCGGCA GCATTTCAAG AGAAGGAACC TTGATGATCA 
     3721 GAGAGACGGC TGAAGAGAAG AAGCGATCTG GCCACAGTGA CAGTAATGGA TTCGCCGGTC 
     3781 ACATCAATCT CCCAGACCTT GTACAGCAGA GCCATTCGCC AGCTGGAACT CCCACTGAGG 
     3841 GGCTGGGCCG CGTCTCCACT CATTCCCAGG AGATGGACTC TGGGGCTGAA TATGGTATAG 
     3901 GGAGCAGCAC CAAAGCCTCT TTCACCCCCT TCGTGGACCC TCGAGTGTAC CAGACATCGC 
     3961 CCACTGATGA AGATGAAGAG GATGATGAGT CTTCAGCTGC TGCCCTGTTT ACTAGCGAAC 
     4021 TTCTTAGGCA AGAACAGGCC AAACTCAATG AAGCGAGGAA GATTTCAGTG GTAAATGTGA 
     4081 ACCCAACAAA CATTCGCCCT CATAGTGACA CACCGGAAAT CAGAAAATAC AAGAAACGCT 
     4141 TCAATTCAGA AATACTTTGT GCAGCTCTAT GGGGTGTGAA CCTTCTGGTG GGGACTGAAA 
     4201 ATGGCCTGAT GCTTTTGGAC AGAAGTGGCC AAGGCAAAGT CTACAACCTA ATCAACCGGA 
     4261 GGCGGTTTCA GCAGATGGAT GTGCTAGAAG GACTAAATGT TCTTGTCACG ATATCAGGAA 
     4321 AGAAGAACAA GCTCCGTGTG TACTATCTCT CATGGTTAAG AAACAGAATC CTGCACAATG 
     4381 ACCCAGAAGT GGAAAAGAAG CAGGGCTGGA TCACTGTCGG TGACTTGGAA GGCTGCATCC 
     4441 ATTACAAAGT CGTTAAATAT GAAAGAATCA AGTTCCTGGT GATTGCCTTA AAGAATGCAG 
     4501 TAGAGATATA TGCGTGGGCC CCTAAACCTT ACCATAAGTT CATGGCATTT AAGTCTTTTG 
     4561 CAGATCTTCA GCATAAGCCT CTGCTCGTTG ACCTCACAGT AGAAGAAGGT CAAAGGTTAA 
     4621 AGGTCATATT TGGCTCACAC ACTGGTTTCC ATGTAATTGA TGTTGATTCT GGAAACTCCT 
     4681 ACGATATCTA TATACCATCC CATATTCAGG GCAATATCAC TCCTCATGCT ATCGTCATCT 
     4741 TGCCTAAAAC AGATGGAATG GAGATGCTTG TCTGCTATGA GGATGAAGGG GTGTACGTGA 
     4801 ACACCTACGG CCGGATCACT AAGGATGTGG TGCTCCAATG GGGAGAAATG CCCACATCTG 
     4861 TGGCCTACAT TCATTCCAAT CAGATAATGG GCTGGGGCGA GAAAGCTATT GAGATCCGGT 
     4921 CAGTGGAAAC AGGACATTTG GATGGAGTGT TTATGCATAA ACGAGCTCAA AGGTTAAAGT 
     4981 TTCTATGTGA AAGAAATGAT AAGGTAAATC CGTTTCAACT TTGCTTTAGT GTTTGCATTT 
     5041 TAAGAGACCA CCAGGTACCT GTGAAACCTT CATTTTCCTT TATACTGACT CAAGTCACAT 
     5101 CTGTGTTCAA TGATCAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5161 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5221 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5281 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5341 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5401 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5461 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5521 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5581 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5641 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5701 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5761 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5821 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5881 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5941 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6001 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6061 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6121 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6181 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6241 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6301 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6361 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6421 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6481 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6541 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6601 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6661 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6721 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6781 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6841 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6901 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6961 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7021 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7081 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7141 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7201 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7261 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7321 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7381 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7441 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7501 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7561 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7621 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7681 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7741 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7801 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7861 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7921 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7981 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8041 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8101 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8161 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8221 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8281 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8341 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8401 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8461 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8521 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8581 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8641 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8701 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8761 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8821 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8881 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8941 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//