LOCUS       MR222144                8961 bp    DNA     circular  SYN 
DEFINITION  Tnik (Myc-DDK-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 1
ACCESSION   NM_026910
VERSION     NM_026910.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5118..5209 
                     /label="Myc-DDK"  
     misc_feature    5239..5824 
                     /label="PolyA signal"  
     misc_feature    5296..5315 
                     /label="Restriction Enzyme"  
     misc_feature    6100..6772 
                     /label="ColE1"  
     misc_feature    7202..7996 
                     /label="Kan/Neo"  
     misc_feature    8031..8333 
                     /label="SV40 ori"  
     misc_feature    8419..8896 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5100..5106 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5100 
                     /label="Tnik"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCAGCTC GCAGCCTGGA TGAAATCGAT CTCTCCGCCC 
     1081 TGAGGGACCC TGCAGGGATC TTTGAGTTGG TGGAACTTGT CGGAAATGGC ACGTATGGTC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC TGCCATTAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACAGGAA CATTGCTACA TACTACGGTG CTTTTATCAA AAAGAACCCT CCTGGCATGG 
     1321 ATGACCAACT CTGGTTGGTT ATGGAGTTCT GTGGTGCTGG CTCTGTCACT GACCTGATCA 
     1381 AGAACACGAA AGGCAACACA TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAGATCT 
     1441 TACGGGGCCT GAGTCACCTG CACCAGCACA AAGTGATTCA TCGAGATATC AAAGGGCAGA 
     1501 ACGTCTTGTT GACTGAAAAT GCAGAGGTTA AGCTAGTGGA TTTTGGAGTG AGTGCCCAGC 
     1561 TTGACCGAAC TGTGGGCAGG AGGAACACGT TCATCGGGAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTCATTGC CTGTGATGAG AACCCGGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGAAT CACCGCCATT GAGATGGCAG AAGGTGCCCC CCCCCTCTGT GACATGCATC 
     1741 CCATGAGAGC CCTCTTCCTC ATTCCACGGA ACCCTGCACC TCGGCTCAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATCGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGGC 
     1861 CAGCCACGGA GCAGTTGATG AAGCACCCAT TCATACGAGA CCAACCTAAT GAGAGGCAGG 
     1921 TCCGCATCCA GCTGAAGGAC CACATTGATC GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACTGAGTA TGAATACAGC GGAAGTGAGG AAGAAGAGGA AGAGAATGAC TCTGGGGAAC 
     2041 CCAGCTCCAT TCTGAACCTA CCAGGCGAGT CAACACTGCG AAGGGACTTC CTGAGACTGC 
     2101 AGCTGGCCAA CAAGGAGCGC TCAGAGGCCC TGCGTCGCCA ACAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA AGAACACAAG CGGCAGCTAC TGGCTGAGCG CCAGAAGCGC ATCGAAGAGC 
     2221 AGAAGGAGCA GAGGCGGAGG CTGGAGGAGC AACAAAGGCG AGAAAAAGAG CTTCGGAAAC 
     2281 AGCAGGAGCG GGAACAGCGC CGGCACTACG AAGAACAGAT GCGTCGGGAG GAGGAGAGGA 
     2341 GGCGTGCCGA ACATGAGCAG GAATACATCA GGCGGCAGCT AGAAGAAGAG CAAAGGCAGT 
     2401 TAGAGATCTT ACAGCAGCAG CTACTGCATG AGCAAGCTCT ACTTCTGGAA TATAAGCGCA 
     2461 AACAATTGGA AGAACAGAGA CAAGCAGAAA GACTGCAGAG GCAGCTAAAG CAAGAGCGGG 
     2521 ACTATCTGGT TTCCCTCCAG CATCAGCGGC AGGAGCAGAG GCCCCTGGAG AAGAAGCCAC 
     2581 TGTACCATTA CAAGGAGGGC ATGAGTCCTA GTGAGAAGCC GGCCTGGGCC AAGGAGGTAG 
     2641 AAGAACGCTC AAGACTCAAC CGACAGAGTT CACCTGCCAT GCCTCACAAG GTTGCCAACA 
     2701 GGATCTCGGA CCCCAACCTG CCCCCAAGAT CAGAGTCCTT CAGCATTAGT GGGGTTCAGC 
     2761 CTGCAAGGAC ACCCCCAATG CTCAGACCTG TTGACCCCCA GATCCCGCAG CTGGTAGCTG 
     2821 TCAAATCCCA GGGACCTGCC TTGACGGCCT CCCAGTCAGT ACATGAGCAA CCCACAAAGG 
     2881 GCCTGTCTGG GTTCCAGGAG GCTCTGAATG TGACCTCTCA CCGGGTCGAG ATGCCACGCC 
     2941 AGAACTCGGA TCCCACCTCA GAAAACCCTC CTCTCCCCAC GAGAATTGAG AAGTTTGACA 
     3001 GAAGCTCTTG GTTACGACAG GAAGAAGACA TTCCACCAAA GGTGCCTCAA AGAACAACTT 
     3061 CTATATCCCC AGCACTAGCC AGAAAGAATT CCCCTGGCAA TGGCAGTGCT CTGGGCCCCA 
     3121 GACTTGGATC TCAGCCCATC AGAGCAAGCA ACCCTGATCT GCGCAGGACA GAGCCAGTCC 
     3181 TGGAGAGTTC CCTGCAGCGG ACAAGCAGTG GCAGTTCCTC CAGCTCCAGC ACTCCCAGCT 
     3241 CCCAGCCCAG CTCCCAAGGA GGCTCTCAAC CTGGCTCCCA AGCAGGATCT AGTGAGCGGT 
     3301 CCAGAGTGCG GGCCAACAGT AAGTCCGAAG GATCACCCGT GCTCCCCCAT GAGCCTTCCA 
     3361 AGGTGAAACC AGAAGAATCC AGAGACATCA CACGGCCCAG TCGGCCAGCT AGCTATAAGA 
     3421 AAGCTATAGA TGAGGATCTG ACGGCATTAG CCAAAGAATT ACGAGAACTC CGCATTGAAG 
     3481 AAACAAACCG CCCCCTGAAG AAAGTGACTG ATTACTCTTC CTCCAGCGAG GAGTCGGAGA 
     3541 GCAGTGAGGA AGAAGAGGAA GATGGAGAGA GTGAGACACA TGACGGGACG GTGGCTGTCA 
     3601 GTGACATACC CAGACTAATA CCCACCGGAG CTCCAGGGAA CAATGAGCAG TACAACATGG 
     3661 GGATGGTCGG GACACATGGG CTGGAAACTT CGCATGCGGA CACCTTTGGC GGCAGCATTT 
     3721 CAAGAGAAGG AACCTTGATG ATCAGAGAGA CGGCTGAAGA GAAGAAGCGA TCTGGCCACA 
     3781 GTGACAGTAA TGGATTCGCC GGTCACATCA ATCTCCCAGA CCTTGTACAG CAGAGCCATT 
     3841 CGCCAGCTGG AACTCCCACT GAGGGGCTGG GCCGCGTCTC CACTCATTCC CAGGAGATGG 
     3901 ACTCTGGGGC TGAATATGGT ATAGGGAGCA GCACCAAAGC CTCTTTCACC CCCTTCGTGG 
     3961 ACCCTCGAGT GTACCAGACA TCGCCCACTG ATGAAGATGA AGAGGATGAT GAGTCTTCAG 
     4021 CTGCTGCCCT GTTTACTAGC GAACTTCTTA GGCAAGAACA GGCCAAACTC AATGAAGCGA 
     4081 GGAAGATTTC AGTGGTAAAT GTGAACCCAA CAAACATTCG CCCTCATAGT GACACACCGG 
     4141 AAATCAGAAA ATACAAGAAA CGCTTCAATT CAGAAATACT TTGTGCAGCT CTATGGGGTG 
     4201 TGAACCTTCT GGTGGGGACT GAAAATGGCC TGATGCTTTT GGACAGAAGT GGCCAAGGCA 
     4261 AAGTCTACAA CCTAATCAAC CGGAGGCGGT TTCAGCAGAT GGATGTGCTA GAAGGACTAA 
     4321 ATGTTCTTGT CACGATATCA GGAAAGAAGA ACAAGCTCCG TGTGTACTAT CTCTCATGGT 
     4381 TAAGAAACAG AATCCTGCAC AATGACCCAG AAGTGGAAAA GAAGCAGGGC TGGATCACTG 
     4441 TCGGTGACTT GGAAGGCTGC ATCCATTACA AAGTCGTTAA ATATGAAAGA ATCAAGTTCC 
     4501 TGGTGATTGC CTTAAAGAAT GCAGTAGAGA TATATGCGTG GGCCCCTAAA CCTTACCATA 
     4561 AGTTCATGGC ATTTAAGTCT TTTGCAGATC TTCAGCATAA GCCTCTGCTT GTTGACCTCA 
     4621 CAGTAGAAGA AGGTCAAAGG TTAAAGGTCA TATTTGGCTC ACACACTGGT TTCCATGTAA 
     4681 TTGATGTTGA TTCTGGAAAC TCCTACGATA TCTATATACC ATCCCATATT CAGGGCAATA 
     4741 TCACTCCTCA TGCTATCGTC ATCTTGCCTA AAACAGACGG AATGGAGATG CTTGTCTGCT 
     4801 ATGAGGATGA AGGGGTGTAC GTGAACACCT ACGGCCGGAT CACTAAGGAT GTGGTGCTCC 
     4861 AATGGGGAGA AATGCCCACA TCTGTGGCCT ACATTCATTC CAATCAGATA ATGGGCTGGG 
     4921 GCGAGAAAGC TATTGAGATC CGGTCAGTGG AAACAGGACA TTTGGATGGA GTGTTTATGC 
     4981 ATAAACGAGC TCAAAGGTTA AAGTTTCTAT GTGAAAGAAA TGATAAGGTA TTTTTTGCAT 
     5041 CCGTGCGATC TGGAGGAAGT AGCCAAGTGT TTTTCATGAC CCTCAACAGA AATTCCATGA 
     5101 TGAACTGGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5161 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5221 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5281 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5341 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5401 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5461 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5521 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5581 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5641 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5701 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5761 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5821 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5881 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5941 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6001 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6061 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6121 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6181 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6241 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6301 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6361 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6421 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6481 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6541 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6601 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6661 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6721 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6781 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6841 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6901 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6961 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7021 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7081 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7141 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7201 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7261 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7321 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7381 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7441 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7501 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7561 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7621 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7681 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7741 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7801 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7861 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7921 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7981 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8041 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8101 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8161 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8341 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8401 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8461 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8521 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8581 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8641 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8701 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8761 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8821 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8881 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8941 CAAAAATTTA ACGCGAATTT T
//