LOCUS       MR222099                7860 bp    DNA     circular  SYN 
DEFINITION  Bnc1 (Myc-DDK-tagged) - Mouse basonuclin 1 (Bnc1)
ACCESSION   NM_007562
VERSION     NM_007562.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4015..4106 
                     /label="Myc-DDK"  
     misc_feature    4136..4721 
                     /label="PolyA signal"  
     misc_feature    4193..4212 
                     /label="Restriction Enzyme"  
     misc_feature    4997..5669 
                     /label="ColE1"  
     misc_feature    6099..6893 
                     /label="Kan/Neo"  
     misc_feature    6928..7230 
                     /label="SV40 ori"  
     misc_feature    7316..7793 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3990..3997 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3990 
                     /label="Bnc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCGGTCG CCGAGCCGGG GCGGACGCGG AGCGGCCCGG GCAGGGGACG 
     1081 CGCGCCGGGA AGGCCGGCTC CGCAGCGGCT GCAGGATGGC TGAGGCTATC GGCTGTACTC 
     1141 TGAACTGCAG TTGCCAATGT TTCAAACCTG GGAAGATAAA CCACCGTCAG TGTGAACAGT 
     1201 GCAGGCACGG ATGGGTGGCT CATGCTCTGA GTAAGCTGAG GATCCCCCCT GTGTATCCAA 
     1261 CAAGCCAAGT GGAAATTGTC CAGTCCAATG TGGTGTTCGA TATTAGCAGC CTCATGCTCT 
     1321 ATGGAACCCA GGCTATCCCT GTTCGCCTGA AAATCCTATT GGACCGGCTC TTCAGTGTGC 
     1381 TGAAGCAAGA TGAGGTTCTA CAGATCCTCC ATGCCCTGGA CTGGACGCTT CAGGATTACA 
     1441 TCCGTGGATA TGTGTTACAG GATGCCTCTG GGAAGGTGCT GGATCACTGG AGCATCATGA 
     1501 CCAGTGAGGA GGAAGTGGCT ACCTTGCAGC AGTTCCTTCG CTTTGGAGAG ACCAAGTCCA 
     1561 TAGTTGAACT CATGGCAATC CAAGAGAAAG AAGAGCAGTC TGTCATCGTA CCGCCCACCA 
     1621 CGGCCAATGT AGACATCAGG GCTTTCATCG AGAGCTGCGG CCACAGGAGT GCCAGCCTCC 
     1681 CTACTCCTGT GGACAAAGGA AGCCCTGGCG GCATGCACCC CTTTGAGAAC CTTATAAGCA 
     1741 ACATGACGTT CATGTTGCCT TTCCAGTTCT TCAACCCTCT GCCTCCTGCT CTGATAGGGT 
     1801 CGCTGCCTGA ACAGTACATG CTGGAGCAGG GGCAGGACCA AAGCCAGGAA CCCAAACAGG 
     1861 AGCTCCATGG GCCCTTCTCG GACAGCAGCT TCTTAACTTC CACCCCATTT CAGGTTGAAA 
     1921 AAGAACAGTG CCTCAACTGT CCAGAAACTG TCCCCCAAAA GGAAGACAGT GCTCACTTAA 
     1981 GCGACTCCAG CTCATATAGC ATTGCCAGTA AGCTTGAAAG GACACAGCTG TCCCCTGAGG 
     2041 CCAAGGTGAA GCCTGAGAGG AACAGCCTCA GTGCTAAGAA GGGCCGGGTG TTCTGCACGG 
     2101 CGTGCGAGAA GACATTCTAT GACAAAGGCA CTCTGAAGAT CCACTACAAC GCCGTCCACC 
     2161 TAAAGATCAA GCACAAGTGC ACGATCGAAG GGTGTAACAT GGTGTTCAGC TCCCTGAGGA 
     2221 GCCGGAACCG ACACAGCGCC AACCCCAACC CTCGCCTGCA CATGCCAATG AACAGAAACA 
     2281 ACAGGGACAA AGATCTCAGG AATAGCCTGA ACCTGGCAAG CTCTGAGACC TACAAGCGCC 
     2341 CAGGTTTCAC AGTGGTGTCT CCAGACTGTG GGCCCCTCCC TGGCTACACT GGTTCCGTGG 
     2401 AGGACTCCAA AGGCCAACCT GCTTTCTCAA GCATTGGGCA AAATGGTGTG CTTTTCCCTA 
     2461 ATCTAAAGAC TGTCCAGCCA GTCCTTCCTT TCTACCGCAG TCCAGCCACT CCCGCGGAGC 
     2521 TGGCCAACAC ACCCGGCATG CTGCCTTCCC TCCCTCTGTT GTCCTCTTCG ATCCCAGAAC 
     2581 AGCTGGTTTC CACAGACATG CCATTCGATG CTCTTCCCAA GAAGAAATCC CGCAAGTCCA 
     2641 GTATGCCTAT CAAAATAGAG AAAGAAGCTG TGGAAATAGC TGAGGAGAAG AGACACAGTC 
     2701 TCAGCTCAGA CGATGAGGTG CCTCTGCAGG TAGTCAGTGA AGACGAGCCA GAGGACAGCA 
     2761 GTCCTAGGTC AGACAGAGTA CCTGAGGAGC AGCATACACA GCTAAGCTTA GAGGAGCCTC 
     2821 TTCCACAAGG AGAAAGAGCC TGCCATCTTG AATCTGTGAT TGAGTCACAT GGAGCTCTCA 
     2881 GCAGAACCCT TGAGCAGACC ACGCTCACTG AGAGGGAGGC TGAACAGAAG GTAGCACTGA 
     2941 GTTCAGTGAT GCCAAGAGAG GTGGAGGATG GTGGCCACGA GCGCCATTTC ACAGCTGGCC 
     3001 TGGTACCCCA AATCCCTTTT CCTGACTACA TGGAGTTGCA GCAGCGCCTG CTTGCTGGGG 
     3061 GACTCTTCGG TGCTTTGTCC AACAGGGGGA TGGCTTTTCC TTTTCTGGAA GAGTCTAAAG 
     3121 AGCTGGAGCA CCTGGGTGAG CATGCACTAG TGAGGCAGAA GGAAGAAGCT TGCTTCCAGT 
     3181 GTGACATCTG CAAGAAGACC TTTAAAAACG CTTGCAGCAT GAAGACGCAT GAGAAGAACA 
     3241 CACACGCCCG AGAGACACAC GCGTGCACAG TGGAGGGCTG CGGGGCTGCT TTCCCTTCCC 
     3301 GTAGGAGCAG AGACAGACAC AGCTCGAACC TAAGCCTCCA CCAGAAAGTA CTGAATGAAG 
     3361 AAGCATTGGA GACTAGTGAG GACCATTTTC GTGCAGCTTA CCTTCTGCAA GATGTGGCCA 
     3421 AGGAGGCCTA TCAGGATGTG GCTTTCACGC CACAAGCATC ACAGACATCT GTCATCTTCA 
     3481 AGGGAACAAG TGGCATGGGC AGTCTGGTTT ACCCAATATC TCAAGTTCAC AGTGCCAGCC 
     3541 TGGAGAGCTA CAACTCTGGC CCCCCTAGCG AGGGCACCAT CCTGGATTTG AGCACTACCT 
     3601 CAAGTATGAA GTCAGAGAGC AGCAGTCATT CCTCCTGGGA CTCTGATGGC GTGAGTGAGG 
     3661 AAGGCACTGC CCTTATGGAG GACAGTGATG GCAACTGTGA AGGACAGAGC CTTGTCTCTG 
     3721 GGGAAGATGA ATACCCTCTC TGTGTCCTAA TGGAGAAGGC AGACCAGAGC CTTGCTAGCC 
     3781 TGCCCTCTGG TTTGCCAATA ACCTGTCATC TCTGCCAAAA GATATACAGT AACAAAGGGA 
     3841 CCTTTAGAGC CCATTACAAA ACCGTGCACC TCCGCCAGCT CCACAAGTGC AAAGTGCCAG 
     3901 GCTGCAACAC GATGTTCTCA TCTGTTCGCA GTAGAAACAG ACACAGTCAG AATCCCAACC 
     3961 TGCACAAGAG CCTGGCTTCA TCTCCAAGCC ACCTCCAGAG CGGACCGACG CGTACGCGGC 
     4021 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4081 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4141 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4201 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4261 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4321 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4381 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4441 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4501 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4561 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4621 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4681 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4741 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4801 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4861 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4921 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4981 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5041 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5101 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5161 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5221 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5281 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5341 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5401 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5461 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5521 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5581 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5641 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5701 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5761 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5821 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5881 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5941 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6001 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6061 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6121 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6181 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6241 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6301 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6361 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6421 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6481 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6541 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6601 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6661 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6721 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6781 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6841 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6901 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6961 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7021 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7081 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7261 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7321 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7381 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7441 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7501 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7561 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7621 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7681 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7741 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7801 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//