LOCUS       MR222089                9400 bp    DNA     circular  SYN 
DEFINITION  Map3k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 1 (Map3k1)
ACCESSION   NM_011945
VERSION     NM_011945.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5557..5648 
                     /label="Myc-DDK"  
     misc_feature    5678..6263 
                     /label="PolyA signal"  
     misc_feature    5735..5754 
                     /label="Restriction Enzyme"  
     misc_feature    6539..7211 
                     /label="ColE1"  
     misc_feature    7641..8435 
                     /label="Kan/Neo"  
     misc_feature    8470..8772 
                     /label="SV40 ori"  
     misc_feature    8858..9335 
                     /label="f1 ori"  
     misc_feature    1060..1067 
                     /label="Restriction Enzyme"  
     misc_feature    5539..5545 
                     /label="Restriction Enzyme"  
     misc_feature    1060..5539 
                     /label="Map3k1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCAGAT CTCAAGCTTA ACTAGCTAGC GGACCGACAT GGCGGCGGCG 
     1081 GCGGGCGATC GCGCCTCGTC GTCGGGATTC CCGGGCGCCG CGGCGGCGAG TCCCGAGGCG 
     1141 GGCGGCGGCG GCGGAGGAGG AGGAGCTCTC CAGGGAAGCG GCGCGCCCGC AGCGGGCGCG 
     1201 GCGGGGCTGC TGCGGGAGCC TGGCAGCGCG GGCCGCGAGC GCGCGGACTG GCGGCGGCGG 
     1261 CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CGGAGCAGCC GCTCTTCCTC 
     1321 GCCGCCGCCT CGCCGCCCTG CCCATCTACT TCCCCGTCGC CGGAGCCCGC GGACGCGGCT 
     1381 GCAGGAGCGA GTCGCTTCCA GCCCGCGGCG GGACCGCCAC CCCCGGGAGC GGCGAGTCGC 
     1441 TGCGGCTCCC ACTCTGCCGA GCTGGCGGCC GCGCGGGACA GCGGCGCCCG GAGCCCCGCG 
     1501 GGGGCCGAGC CGCCCTCTGC AGCGGCCCCC TCCGGTCGAG AGATGGAGAA TAAAGAAACC 
     1561 CTCAAAGGAC TGCACAAGAT GGAGGATCGC CCGGAGGAGA GAATGATCCG GGAGAAGCTC 
     1621 AAGGCGACCT GTATGCCCGC CTGGAAGCAC GAGTGGTTGG AGAGGAGGAA CAGGAGAGGC 
     1681 CCTGTGGTGG TGAAGCCAAT CCCTATTAAA GGAGATGGAT CTGAAATGAA TAACTTGGCA 
     1741 GCTGAGCCCC AAGGAGAGGG CCAGGCAGGT TCCGCTGCAC CAGCCCCCAA GGGCCGACGA 
     1801 AGCCCATCTC CTGGCAGCTC TCCGTCAGGG CGCTCGGTGA AGCCGGAATC CCCAGGAGTG 
     1861 AGACGGAAAC GAGTGTCCCC GGTGCCTTTC CAGAGTGGCA GAATCACACC ACCCCGAAGA 
     1921 GCCCCGTCAC CGGATGGCTT CTCCCCGTAC AGCCCAGAGG AGACGAGCCG CCGCGTGAAC 
     1981 AAAGTGATGA GAGCCAGGCT GTACCTGCTG CAGCAGATAG GACCCAACTC TTTCCTGATT 
     2041 GGAGGAGACA GTCCAGACAA TAAATACCGG GTGTTTATTG GGCCACAGAA CTGCAGCTGT 
     2101 GGGCGTGGAG CATTCTGTAT TCACCTCTTG TTTGTCATGC TCCGGGTGTT TCAGCTAGAA 
     2161 CCCTCTGACC CCATGTTATG GAGAAAAACT TTAAAAAATT TCGAGGTTGA GAGTTTGTTC 
     2221 CAGAAATACC ACAGTAGGCG TAGCTCGAGA ATCAAAGCTC CATCCCGGAA CACCATCCAG 
     2281 AAGTTTGTGT CACGCATGTC AAATTGTCAC ACACTGTCAT CGTCTAGCAC ATCCACATCT 
     2341 AGTTCAGAAA ACAGCATCAA GGATGAAGAG GAGCAGATGT GTCCCATCTG CTTGCTGGGC 
     2401 ATGCTGGATG AGGAGAGCCT GACTGTGTGT GAAGATGGCT GCAGGAACAA GCTGCACCAC 
     2461 CATTGCATGT CCATCTGGGC GGAAGAGTGT AGAAGAAATA GAGAGCCTTT AATATGTCCC 
     2521 CTTTGTAGAT CTAAGTGGAG ATCCCATGAC TTCTACAGCC ATGAGTTATC AAGCCCCGTG 
     2581 GAGTCCCCCA CCTCCCTGCG AGCTGTCCAG CAGCCATCCT CCCCACAGCA GCCCGTGGCC 
     2641 GGATCACAGC GAAGGAATCA GGAGAGCAGT TTTAACCTTA CTCATTTTGG AACCCAGCAG 
     2701 ATTCCTTCCG CTTACAAAGA TTTGGCCGAG CCATGGATTC AGGTGTTTGG AATGGAACTC 
     2761 GTTGGCTGCT TATTCTCTAG AAACTGGAAC GTAAGGGAAA TGGCCCTTAG GCGTCTTTCC 
     2821 CACGACGTTA GTGGGGCCCT GTTGTTGGCA AACGGGGAGA GCACTGGAAA CTCTGGAGGC 
     2881 GGCAGTGGGG GCAGCTTAAG CGCGGGAGCG GCCAGCGGGT CCTCCCAGCC CAGCATCTCA 
     2941 GGGGATGTGG TGGAGGCGTG CTGCAGTGTC CTGTCTATAG TCTGCGCTGA CCCTGTCTAC 
     3001 AAAGTGTACG TTGCTGCTTT AAAAACATTG AGAGCCATGC TGGTATACAC TCCTTGCCAC 
     3061 AGTCTGGCAG AAAGAATCAA ACTTCAGAGA CTCCTCCGGC CAGTTGTAGA CACTATCCTT 
     3121 GTCAAGTGTG CAGATGCCAA CAGCCGCACG AGTCAGCTGT CCATATCTAC AGTGCTGGAA 
     3181 CTCTGCAAGG GCCAAGCAGG AGAGCTGGCG GTTGGGAGAG AAATACTTAA AGCTGGGTCC 
     3241 ATCGGGGTTG GTGGTGTCGA TTACGTCTTA AGTTGTATCC TTGGAAACCA AGCTGAATCA 
     3301 AACAACTGGC AAGAACTGCT GGGTCGCCTC TGTCTTATAG ACAGGTTGCT GTTGGAATTT 
     3361 CCTGCTGAAT TCTATCCTCA TATTGTCAGT ACTGATGTCT CACAAGCTGA GCCTGTTGAA 
     3421 ATCAGGTACA AGAAGCTGCT CTCCCTCTTA ACCTTTGCCT TGCAATCCAT TGACAATTCC 
     3481 CACTCGATGG TTGGCAAGCT CTCTCGGAGG ATATATCTGA GCTCTGCCAG GATGGTGACC 
     3541 GCAGTGCCCG CTGTGTTTTC CAAGCTGGTA ACCATGCTTA ATGCTTCTGG CTCCACCCAC 
     3601 TTCACCAGGA TGCGCCGGCG TCTGATGGCT ATCGCGGATG AGGTAGAAAT TGCCGAGGTC 
     3661 ATCCAGCTGG GTGTGGAGGA CACTGTGGAT GGGCATCAGG ACAGCTTACA GGTGCTGGCC 
     3721 CCCGCCAGCT GTCTAGAAAA CAGCTCCCTT GAGCACACAG TCCATAGAGA GAAAACTGGA 
     3781 AAAGGACTAA GTGCTACGAG ACTGAGTGCC AGCTCGGAGG ACATTTCTGA CAGACTGGCC 
     3841 GGCGTCTCTG TAGGACTTCC CAGCTCAACA ACAACAGAAC AACCAAAGCC AGCGGTTCAA 
     3901 ACAAAAGGCA GACCCCACAG TCAGTGTTTG AACTCCTCCC CTTTGTCTCA TGCTCAATTA 
     3961 ATGTTCCCAG CACCATCAGC CCCTTGTTCC TCTGCCCCGT CTGTCCCAGA TATTTCTAAG 
     4021 CACAGACCCC AGGCATTTGT TCCCTGCAAA ATACCTTCCG CATCTCCTCA GACACAGCGC 
     4081 AAGTTCTCTC TACAATTCCA GAGGAACTGC TCTGAACACC GAGACTCAGA CCAGCTCTCC 
     4141 CCAGTCTTCA CTCAGTCAAG GCCCCCACCC TCCAGTAACA TACACAGGCC AAAGCCATCC 
     4201 CGACCCGTTC CGGGCAGTAC AAGCAAACTA GGGGACGCCA CAAAAAGTAG CATGACACTT 
     4261 GATCTGGGCA GTGCTTCCAG GTGTGACGAC AGCTTTGGCG GCGGCGGCAA CAGTGGCAAC 
     4321 GCCGTCATAC CCAGCGAAGA GACAGTGTTC ACGCCGGTGG AGGACAAGTG CAGGTTAGAT 
     4381 GTGAACACCG AGCTCAACTC CAGCATCGAG GACCTTCTTG AAGCATCCAT GCCTTCAAGT 
     4441 GACACGACAG TCACTTTCAA GTCCGAAGTC GCCGTCCTCT CTCCGGAAAA GGCCGAAAAT 
     4501 GACGACACCT ACAAAGACGA CGTCAATCAT AATCAAAAGT GCAAAGAAAA GATGGAAGCT 
     4561 GAAGAGGAGG AGGCTTTAGC GATCGCCATG GCGATGTCAG CGTCTCAGGA TGCCCTCCCC 
     4621 ATCGTCCCTC AGCTGCAGGT GGAAAATGGA GAAGATATTA TCATCATTCA GCAGGACACA 
     4681 CCAGAAACTC TTCCAGGACA TACCAAAGCG AAACAGCCTT ACAGAGAAGA CGCTGAGTGG 
     4741 CTGAAAGGCC AGCAGATAGG CCTCGGAGCA TTTTCTTCCT GTTACCAAGC ACAGGATGTG 
     4801 GGGACTGGGA CTTTAATGGC TGTGAAACAG GTGACGTACG TCAGAAACAC ATCCTCCGAG 
     4861 CAGGAGGAGG TGGTGGAAGC GTTGAGGGAA GAGATCCGGA TGATGGGTCA CCTCAACCAT 
     4921 CCAAACATCA TCCGGATGCT GGGGGCCACG TGCGAGAAGA GCAACTACAA CCTCTTCATT 
     4981 GAGTGGATGG CGGGAGGATC TGTGGCTCAC CTCTTGAGTA AATACGGAGC TTTCAAGGAG 
     5041 TCAGTCGTCA TTAACTACAC TGAGCAGTTA CTCCGTGGCC TTTCCTATCT CCACGAGAAC 
     5101 CAGATCATTC ACAGAGACGT CAAAGGTGCC AACCTGCTCA TTGACAGCAC CGGTCAGAGG 
     5161 CTGAGAATTG CAGACTTTGG AGCTGCTGCC AGGTTGGCAT CAAAAGGAAC CGGTGCAGGA 
     5221 GAGTTCCAGG GACAGTTACT GGGGACAATT GCATTCATGG CGCCTGAGGT CCTAAGAGGT 
     5281 CAGCAGTATG GTAGGAGCTG TGATGTATGG AGTGTTGGCT GCGCCATTAT AGAAATGGCT 
     5341 TGTGCAAAAC CACCTTGGAA TGCAGAAAAA CACTCCAATC ATCTCGCCTT GATATTTAAG 
     5401 ATTGCTAGCG CAACTACTGC ACCGTCCATC CCGTCACACC TGTCCCCGGG TCTGCGCGAC 
     5461 GTGGCGCTGC GCTGCTTAGA ACTTCAGCCT CAGGACCGGC CTCCGTCCAG AGAGCTGCTG 
     5521 AAACATCCGG TCTTCCGTAC CACGTGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC 
     5581 TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT 
     5641 TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA 
     5701 CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT 
     5761 CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG 
     5821 GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG 
     5881 GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC 
     5941 CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG 
     6001 CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC 
     6061 CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT 
     6121 GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA 
     6181 CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA 
     6241 TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT 
     6301 TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     6361 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     6421 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     6481 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     6541 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     6601 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     6661 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     6721 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     6781 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     6841 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     6901 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     6961 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7021 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7081 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7141 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC 
     7201 CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG 
     7261 AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC 
     7321 TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG 
     7381 ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG 
     7441 GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA 
     7501 TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA 
     7561 ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT 
     7621 GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA 
     7681 GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA 
     7741 TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC 
     7801 CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT 
     7861 ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC 
     7921 CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC 
     7981 CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG 
     8041 GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT 
     8101 GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC 
     8161 GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG 
     8221 AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC 
     8281 ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC 
     8341 GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC 
     8401 CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA 
     8461 TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT 
     8521 TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC 
     8581 AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA 
     8641 GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG 
     8701 GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC 
     8761 TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT 
     8821 GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 
     8881 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 
     8941 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 
     9001 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC 
     9061 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 
     9121 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 
     9181 TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 
     9241 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 
     9301 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT 
     9361 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//