LOCUS       MR222086                7356 bp    DNA     circular  SYN 
DEFINITION  Nlgn3 (Myc-DDK-tagged) - Mouse neuroligin 3 (Nlgn3)
ACCESSION   NM_172932
VERSION     NM_172932.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3513..3604 
                     /label="Myc-DDK"  
     misc_feature    3634..4219 
                     /label="PolyA signal"  
     misc_feature    3691..3710 
                     /label="Restriction Enzyme"  
     misc_feature    4495..5167 
                     /label="ColE1"  
     misc_feature    5597..6391 
                     /label="Kan/Neo"  
     misc_feature    6426..6728 
                     /label="SV40 ori"  
     misc_feature    6814..7291 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3495..3501 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3495 
                     /label="Nlgn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCTGCAG CCCTCGCTGT CCCTGAGCCC CACGCCCACA GTTGGCCGGA 
     1081 GCCTGTGCCT CACCCTGGGC TTCCTCAGTT TGGTGCTGAG GGCCAGTACC CAGGCCCCGG 
     1141 CACCCACAGT CAATACTCAC TTTGGGAAGC TAAGGGGTGC CAGAGTACCA TTGCCCAGTG 
     1201 AAATCCTGGG TCCTGTGGAC CAATACCTGG GGGTACCCTA CGCAGCTCCC CCGATCGGCG 
     1261 AGAAACGTTT CCTGCCCCCT GAACCACCCC CATCCTGGTC GGGCATCCGG AACGCCACAC 
     1321 ACTTTCCCCC AGTGTGCCCC CAGAACATCC ACACAGCTGT GCCCGAAGTC ATGCTGCCAG 
     1381 TCTGGTTCAC TGCCAACTTG GATATCGTCG CCACTTATAT CCAGGAGCCC AACGAAGATT 
     1441 GCCTCTATCT GAATGTGTAT GTGCCCACGG AAGATGGATC CGGCGCTAAG AAACAGGGCG 
     1501 AGGACTTAGC GGATAATGAC GGGGATGAAG ATGAAGACAT CCGAGACAGT GGTGCTAAAC 
     1561 CTGTCATGGT CTACATCCAC GGAGGCTCTT ACATGGAAGG AACAGGCAAC ATGATTGATG 
     1621 GCAGTGTTCT TGCAAGTTAC GGCAACGTCA TCGTCATCAC CCTCAACTAT CGGGTCGGGG 
     1681 TGCTAGGTTT CCTGAGCACT GGAGATCAGG CTGCCAAGGG CAACTATGGG CTCCTTGATC 
     1741 AAATCCAGGC CCTTCGCTGG GTGAGTGAGA ATATTGCCTT CTTTGGAGGA GATCCCCGTA 
     1801 GAATTACTGT CTTTGGCTCT GGCATCGGTG CATCCTGTGT CAGTCTCCTT ACACTGTCTC 
     1861 ATCATTCTGA GGGGCTTTTC CAGAGGGCCA TCATCCAAAG TGGCTCTGCT CTATCTAGCT 
     1921 GGGCTGTGAA CTACCAACCA GTGAAGTATA CCAGCTTGCT GGCAGACAAA GTGGGCTGTA 
     1981 ACGTCCTGGA CACTGTGGAT ATGGTGGATT GTCTTCGACA AAAGAGTGCC AAGGAGCTGG 
     2041 TAGAACAGGA CATTCAGCCA GCCCGCTACC ATGTGGCTTT TGGCCCTGTG ATTGATGGTG 
     2101 ATGTCATTCC TGATGACCCT GAGATCCTTA TGGAGCAGGG AGAGTTCCTC AACTATGATA 
     2161 TCATGCTAGG CGTCAACCAG GGTGAGGGTC TCAAGTTTGT GGAAGGGGTG GTGGACCCCG 
     2221 AGGATGGTGT CTCGGGCACT GACTTTGACT ACTCTGTCTC CAATTTTGTG GACAATCTGT 
     2281 ATGGCTATCC TGAGGGTAAG GACACCCTGC GGGAGACTAT CAAGTTCATG TATACGGACT 
     2341 GGGCAGACCG AGACAACCCT GAGACCCGCC GTAAAACACT GGTGGCACTC TTCACTGACC 
     2401 ACCAGTGGGT GGAGCCTTCA GTGGTGACAG CCGATCTGCA CGCCCGCTAT GGCTCACCTA 
     2461 CCTACTTCTA CGCCTTCTAC CATCACTGCC AGAGCCTCAT GAAGCCCGCA TGGTCAGATG 
     2521 CAGCACACGG GGATGAAGTG CCCTATGTTT TTGGTGTCCC TATGGTAGGT CCCACTGACC 
     2581 TTTTCCCCTG CAACTTCTCC AAGAATGATG TTATGCTCAG TGCTGTCGTC ATGACCTATT 
     2641 GGACCAACTT TGCCAAGACC GGGGATCCCA ACAAGCCGGT ACCCCAGGAT ACCAAGTTCA 
     2701 TTCACACCAA GGCCAACCGC TTTGAGGAAG TGGCCTGGTC CAAATACAAT CCCCGAGACC 
     2761 AGCTCTACCT TCACATCGGG CTGAAACCAA GGGTTCGTGA TCATTACCGG GCCACAAAGG 
     2821 TAGCCTTTTG GAAACACCTG GTGCCCCACC TGTACAACCT GCATGACATG TTCCACTATA 
     2881 CATCCACGAC CACCAAAGTG CCGCCCCCGG ACACCACCCA CAGCTCCCAC ATCACCCGTA 
     2941 GGCCCAACGG CAAGACCTGG AGCACCAAGC GGCCGGCGAT TTCACCTGCC TACAGCAATG 
     3001 AGAATGCCCC TGGGTCCTGG AATGGGGACC AGGATGCGGG GCCACTCCTG GTTGAGAACC 
     3061 CTCGAGACTA CTCCACTGAA TTAAGTGTCA CTATCGCTGT GGGGGCCTCC CTCCTGTTTC 
     3121 TCAATGTGTT GGCCTTTGCT GCCCTCTATT ACCGTAAGGA CAAACGGCGC CAGGAGCCCC 
     3181 TGAGGCAGCC TAGCCCCCAA AGGGGAACTG GTGCCCCTGA ATTGGGAACT GCTCCGGAGG 
     3241 AGGAGCTGGC AGCATTACAG TTGGGTCCCA CTCACCATGA ATGTGAGGCC GGTCCCCCAC 
     3301 ATGACACACT TCGCCTCACA GCACTGCCCG ACTATACCCT GACCCTGCGG CGCTCCCCTG 
     3361 ATGACATCCC ACTCATGACT CCCAACACCA TCACTATGAT TCCTAATTCC CTGGTTGGGT 
     3421 TGCAGACCTT GCACCCCTAT AACACCTTTG CCGCAGGGTT CAACAGTACT GGGCTGCCCC 
     3481 ACTCACACTC CACTACCCGT GTAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3541 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3601 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3661 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3721 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3781 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3841 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3901 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3961 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4021 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4081 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4141 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4201 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4261 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4321 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4381 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4441 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4501 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4561 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4621 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4681 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4741 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4801 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4861 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4921 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4981 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5041 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5101 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5161 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5221 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5281 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5341 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5401 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5461 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5521 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5581 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5641 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5701 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5761 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5821 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5881 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5941 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6001 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6061 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6121 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6181 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6241 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6301 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6361 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6421 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6481 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6541 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6601 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6661 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6721 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6781 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6841 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6901 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6961 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7021 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7081 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7141 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7201 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7261 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7321 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//