LOCUS       MR222084                8997 bp    DNA     circular  SYN 
DEFINITION  Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 3
ACCESSION   NM_001012623
VERSION     NM_001012623.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5152..5243 
                     /label="Myc-DDK"  
     misc_feature    5273..5858 
                     /label="PolyA signal"  
     misc_feature    5330..5349 
                     /label="Restriction Enzyme"  
     misc_feature    6134..6806 
                     /label="ColE1"  
     misc_feature    7236..8030 
                     /label="Kan/Neo"  
     misc_feature    8065..8367 
                     /label="SV40 ori"  
     misc_feature    8453..8930 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5142..5150 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5142 
                     /label="Rims1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTCGGCC GTGGGGCCCC GAGGTCCTCG CCCACCCACG GTGCCTCCCC 
     1081 CTATGCAAGA ACTGCCCGAC CTGAGCCACC TGACCGAGGA GGAGAGGAAC ATTATCATGG 
     1141 CAGTGATGGA CCGGCAGAAG GAAGAGGAGG AAAAAGAAGA GGCCATGCTC AAGTGTGTTG 
     1201 TCAGGGACAT GGCGAAGCCT GCTGCCTGCA AAACACCAAG AAATGCTGAA AGCCAGCCCC 
     1261 ATCAACCACC ACTGAACATT TTCAGATGTG TCTGTGTTCC CAGAAAGCCA AGCAGCGAAG 
     1321 AGGGAGGCCC AGACAGAAAC TGGAGATTGC ATCAACAGTT TGAAAGCTAC AAGGAACAAG 
     1381 TGAGAAAAAT CGGAGAGGAA GCCAGGCGTT ACCAGGGCGA GCACAAAGAT GATGCCCCGA 
     1441 CGTGTGGAAT CTGTCATAAG ACAAAGTTTG CTGATGGATG TGGCCATCTC TGCTCCTATT 
     1501 GTCGCACCAA GTTCTGTGCA CGCTGTGGAG GCCGAGTGTC TCTGCGATCG AACAATGAGG 
     1561 ACAAAGTGGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAGATA TTAACGAAAT 
     1621 CTGGAGCGTG GTTCTTCGGA AGTGGTCCTC AGCAGCCCAG TCAAGATGGG ACTCTGAGTG 
     1681 ACACGGCTAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCTCGG CTCCAAGAGC 
     1741 GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTTCCC 
     1801 ATGGTGCACC ACTGGACAGG AACAAAGGGG CGGAGCCCTC ACAGCAAGCC TTGGGTCCTG 
     1861 AACAGAAGCA GGCATCAAGG TCAAGAAGTG AGCCACCAAG GGAAAGGAAG AAGGCTCCAG 
     1921 GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGTGA GCGCAAACGT GTCCCCAAGT 
     1981 CTGTGGTGCA ACCCGGGGAA GGGACCGCGG ATGAACGGGA GAGGAAGGAG AGGCGGGAAA 
     2041 CCCGCAGGCT GGAGAAAGGG CGCTCTCAGG ACTACCCGGA CCGGCTCGAG AAACGCGAGG 
     2101 ATGGCAGGGT GGCTGAAGAC GAAAAGCAGA GGAAGGAGGA GGAGGGCGTG TCCACGCCGG 
     2161 AGTACACGAG CTGCGAGGAC GTGGAGCTGG AGAGCGAGAG CGTGAGCGAG AAAGGTGACT 
     2221 TGGACTACTG GTTGGATCCT GCCACGTGGC ATAGCAGGGA AACGTCGCCT ATTAGTTCGC 
     2281 ATCCTGTAAC GTGGCAGCCA TCTAAAGAGG GGGACCGATT AATCGGCCGT GTTATTCTTA 
     2341 ACAAAAGAAC AACCATGCCC AAAGAATCAG GTGCATTATT GGGTCTCAAG GTGGTTGGAG 
     2401 GAAAAATGAC GGACTTAGGG CGTCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGTAGCC 
     2461 TGGCAGATGT TGTCGGACAC CTAAGAGCAG GGGATGAAGT TCTAGAGTGG AATGGTAAAC 
     2521 CCCTGCCGGG AGCAACAAAC GAAGAAGTTT ACAACATTAT TTTAGAATCA AAATCAGAAC 
     2581 CTCAAGTTGA AATTATTGTT TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT 
     2641 CCCACCCTCC TCTGGAGTCC AGTTCAAGTT CCTTTGAATC TCAGAAGATG GAAAGGCCTT 
     2701 CCATTTCTGT TATTTCTCCA ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC 
     2761 CAGGGCAACT CTCAGTGAAG CTGTGGTATG ATAAGGTGGG GCACCAGCTG ATTGTAAATG 
     2821 TTCTACAAGC AACAGATCTA CCCCCTAGAG TAGATGGGCG TCCCAGAAAT CCCTATGTAA 
     2881 AAATGTATTT TCTTCCAGAT AGAAGCGACA AGAGTAAAAG AAGAACCAAA ACGGTAAAGA 
     2941 AACTTCTAGA GCCAAAATGG AATCAAACAT TTGTCTACTC ACACGTACAT CGTCGAGATT 
     3001 TTCGAGAGCG AATGTTAGAA ATTACTGTGT GGGACCAGCC AAGAGTACAG GACGAGGAGA 
     3061 GTGAATTTCT AGGAGAGATC CTCATAGAAT TGGAAACAGC ACTTTTAGAT GATGAGCCCC 
     3121 ATTGGTATAA ACTGCAGACA CATGATGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT 
     3181 TCATGCCCAG GCGGCATATC CACGGAGAAA GCTCCAGCAA AAAGCTACAA AGATCTCAGC 
     3241 GAATCAGTGA TAGTGACATC TCAGATTATG AGGTTGATGA TGGTATTGGC GTAGTGCCTC 
     3301 CAGTGGGTTA TAGAGCTAGT GCTAGAGAGA GTAAAGCCAC CACGTTAACA GTGCCAGAGC 
     3361 AGCAAAGAAC CACACATCAC CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG 
     3421 GAAGGCCTCG TTCACGTTTA CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATCCATC 
     3481 CAACACGAAG GTCACGTTCT CCAACCCGAC ACCATGATGC CTCCCGAAGC CTGGCCGATC 
     3541 ACAGATCACG ACATGCGGAA AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC 
     3601 TGCCCAGAGC AAAACGAGGA CGAAGTGCAG AATGCCTACA CATGACCAGT GAACTGCAGC 
     3661 CCTCTCTTGA CAGGGCTAGG AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC 
     3721 ATTCACCAGA ACGAGAAAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAAACAGT 
     3781 CTAGGAAAGG CACAGCCTCT GATGCAGACA GGACGCACCG ACAAGGAAGC CCCACCCAGT 
     3841 CACCTCCAGC TGACACATCC TTTGGCAGTC GCCGCGGAAG ACAGCTCCCA CAGGTGCCGG 
     3901 TCCGAAGCGG CAGTATAGAA CAAGCGAGCT TAGTAGTGGA GGAACGAACG AGACAGATGA 
     3961 AAATGAAGGT ACACCGATTT AAGCAGACAA CAGGGTCTGG GTCTAGTCAA GAACTTGACC 
     4021 ACGAGCAATA CTCCAAGTAC AACATACATA AAGATCAGTA CAGAAGCTGT GATAACGCGT 
     4081 CCGCCAAGTC ATCAGATAGT GATGTCAGTG ATGTGTCCGC CATTTCCAGA GCCAGCAGCA 
     4141 CCTCACGCCT CAGCAGCACA AGCTTTATGT CAGAGCAGTC TGAGCGCCCC AGGGGCAGGA 
     4201 TCAGTTCATT TACCCCCAAA ATGCAAGGCA GACGGATGGG GACTTCAGGA AGAGCCATCA 
     4261 TCAAGAGCAC CAGTGTAAGT GGAGAGATAT ATACACTGGA GCACAATGAC GGCAGCCAGT 
     4321 CCGACACGGC CGTGGGTACC GTTGGAGCTG GTGGAAAGAA GCGGAGGTCC AGCCTGAGTG 
     4381 CCAAAGTGGT AGCCATTGTG TCTCGAAGAA GCAGAAGCAC ATCACAGCTC AGCCAGACAG 
     4441 AGTCCGGCCA CAAGAAGTTA AAAAGCACCA TTCAGAGGAG TACGGAAACG GGAATGGCGG 
     4501 CTGAAATGCG GAAGATGGTG AGACAGCCCA GCCGGGAGTC CACGGATGGC AGCATCAACA 
     4561 GTTATAGCTC AGAAGGAAAC TTAATATTTC CTGGAGTTCG CGTGGGACCT GACAGTCAGT 
     4621 TCAGTGATTT CCTTGATGGA CTGGGGCCAG CCCAGCTTGT CGGCCGCCAA ACCCTAGCCA 
     4681 CCCCAGCCAT GGGCGATATC CAAATCGGGA TGGAGGATAA GAAGGGTCAG TTGGAGGTTG 
     4741 AGGTTATCAG AGCCCGGAGC CTTACACAAA AACCTGGCTC CAAATCTACA CCTGCTCCCT 
     4801 ATGTGAAAGT TTATCTTTTG GAAAATGGAG CCTGTATTGC CAAAAAGAAG ACAAGAATTG 
     4861 CACGGAAAAC TCTCGATCCC TTGTATCAGC AGTCCCTGGT TTTTGATGAA AGTCCACAGG 
     4921 GTAAAGTTCT TCAGGTGATT GTCTGGGGTG ACTATGGAAG AATGGACCAC AAATGCTTTA 
     4981 TGGGTGTGGC TCAAATCTTG TTGGAAGAAC TTGATCTGTC CAGCATGGTG ATTGGATGGT 
     5041 ATAAATTGTT CCCTCCATCC TCCCTGGTGG ATCCCACACT CACTCCCCTG ACCCGCCGGG 
     5101 CTTCCCAATC ATCACTGGAA AGTTCGTCTG GGCCTCCCTG CATCCGGTCA ACGCGGCCGC 
     5161 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5221 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5281 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5341 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5401 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5461 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5521 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5581 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5641 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5701 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5761 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5821 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5881 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5941 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6001 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6061 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6121 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6181 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6241 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6301 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6361 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6421 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6481 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6541 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6601 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6661 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6721 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6781 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6841 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6901 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6961 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7021 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7081 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7141 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7201 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7261 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7321 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7381 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7441 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7501 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7561 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7621 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7681 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7741 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7801 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7861 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7921 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7981 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8041 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8101 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8161 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8221 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8281 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8341 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8401 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8461 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8521 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8581 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8641 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8701 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8761 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8821 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8881 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8941 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//