LOCUS MR222017 6774 bp DNA circular SYN DEFINITION Dmpk (Myc-DDK-tagged) - Mouse dystrophia myotonica-protein kinase (Dmpk), transcript variant 1 ACCESSION NM_032418 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2931..3022 /label="Myc-DDK" misc_feature 3052..3637 /label="PolyA signal" misc_feature 3109..3128 /label="Restriction Enzyme" misc_feature 3913..4585 /label="ColE1" misc_feature 5015..5809 /label="Kan/Neo" misc_feature 5844..6146 /label="SV40 ori" misc_feature 6232..6709 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2913..2919 /label="Restriction Enzyme" misc_feature 1020..2913 /label="Dmpk" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GTCAGCCGAA GTGCGGCTGA GGCAGCTCCA GCAGCTGGTG CTGGACCCAG 1081 GCTTCCTGGG ACTGGAGCCC CTGCTCGACC TTCTCCTGGG CGTCCACCAG GAGCTGGGTG 1141 CCTCTCACCT AGCCCAGGAC AAGTATGTGG CCGACTTCTT GCAGTGGGTG GAGCCCATTG 1201 CAGCAAGGCT TAAGGAGGTC CGACTGCAGA GGGATGATTT TGAGATTTTG AAGGTGATCG 1261 GGCGTGGGGC GTTCAGCGAG GTAGCGGTGG TGAAGATGAA ACAGACGGGC CAAGTGTATG 1321 CCATGAAGAT TATGAATAAG TGGGACATGC TGAAGAGAGG CGAGGTGTCG TGCTTCCGGG 1381 AAGAAAGGGA TGTATTAGTG AAAGGGGACC GGCGCTGGAT CACACAGCTG CACTTTGCCT 1441 TCCAGGATGA GAACTACCTG TACCTGGTCA TGGAATACTA CGTGGGCGGG GACCTGCTAA 1501 CGCTGCTGAG CAAGTTTGGG GAGCGGATCC CCGCCGAGAT GGCTCGCTTC TACCTGGCCG 1561 AGATTGTCAT GGCCATAGAC TCCGTGCACC GGCTGGGCTA CGTGCACAGG GACATCAAAC 1621 CAGATAACAT TCTGCTGGAC CGATGTGGGC ACATTCGCCT GGCAGACTTC GGCTCCTGCC 1681 TCAAACTGCA GCCTGATGGA ATGGTGAGGT CGCTGGTGGC TGTGGGCACC CCGGACTACC 1741 TGTCTCCTGA GATTCTGCAG GCCGTTGGTG GAGGGCCTGG GGCAGGCAGC TACGGGCCAG 1801 AGTGTGACTG GTGGGCACTG GGCGTGTTCG CCTATGAGAT GTTCTATGGG CAGACCCCCT 1861 TCTACGCGGA CTCCACAGCC GAGACATATG CCAAGATTGT GCACTACAGG GAACACTTGT 1921 CGCTGCCGCT GGCAGACACA GTTGTCCCCG AGGAAGCTCA GGACCTCATT CGTGGGCTGC 1981 TGTGTCCTGC TGAGATAAGG CTAGGTCGAG GTGGGGCAGG TGATTTCCAG AAACATCCTT 2041 TCTTCTTTGG CCTTGATTGG GAGGGTCTCC GAGACAGTGT ACCCCCCTTT ACACCAGACT 2101 TCGAGGGTGC CACGGACACA TGCAATTTCG ATGTGGTGGA GGACCGGCTC ACTGCCATGG 2161 TGAGCGGGGG CGGGGAGACG CTGTCAGACA TGCAGGAAGA CATGCCCCTT GGGGTGCGCC 2221 TGCCCTTCGT GGGCTACTCC TACTGCTGCA TGGCCTTCAG AGACAATCAG GTCCCGGACC 2281 CCACCCCTAT GGAACTAGAG GCCCTGCAGT TGCCTGTGTC AGACTTGCAA GGGCTTGACT 2341 TGCAGCCCCC AGTGTCCCCA CCGGATCAAG TGGCTGAAGA GGCTGACCTA GTGGCTGTCC 2401 CTGCCCCTGT GGCTGAGGCA GAGACCACGG TAACGCTGCA GCAGCTCCAG GAAGCCCTGG 2461 AAGAAGAGGT TCTCACCCGG CAGAGCCTGA GCCGCGAGCT GGAGGCCATC CGGACCGCCA 2521 ACCAGAACTT CTCCAGCCAA CTACAGGAGG CCGAGGTCCG AAACCGAGAC CTGGAGGCGC 2581 ATGTTCGGCA GCTACAGGAA CGGATGGAGA TGCTGCAGGC CCCAGGAGCC GCAGCCATCA 2641 CGGGGGTCCC CAGTCCCCGG GCCACGGATC CACCTTCCCA TCTAGATGGC CCCCCGGCCG 2701 TGGCTGTGGG CCAGTGCCCG CTGGTGGGGC CAGGCCCCAT GCACCGCCGT CACCTGCTGC 2761 TCCCTGCCAG GATCCCTAGG CCTGGCCTAT CCGAGGCGCG TTGCCTGCTC CTGTTCGCCG 2821 CTGCTCTGGC TGCTGCCGCC ACACTGGGCT GCACTGGGTT GGTGGCCTAT ACCGGCGGTC 2881 TCACCCCAGT CTGGTGTTTC CCGGGAGCCA CCTTCGCCCC CACGCGTACG CGGCCGCTCG 2941 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 3001 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 3061 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 3121 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 3181 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 3241 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 3301 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 3361 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 3421 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 3481 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 3541 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 3601 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 3661 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 3721 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 3781 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 3841 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 3901 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 3961 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 4021 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 4081 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 4141 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 4201 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 4261 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 4321 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 4381 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 4441 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 4501 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 4561 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 4621 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 4681 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 4741 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 4801 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 4861 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 4921 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 4981 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 5041 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 5101 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 5161 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 5221 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 5281 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 5341 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 5401 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 5461 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 5521 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 5581 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 5641 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 5701 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 5761 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 5821 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 5881 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 5941 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 6001 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 6061 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 6121 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 6181 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 6241 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 6301 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 6361 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 6421 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 6481 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 6541 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 6601 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 6661 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 6721 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT //