LOCUS       MR221956                6102 bp    DNA     circular  SYN 
DEFINITION  Ambn (Myc-DDK-tagged) - Mouse ameloblastin (Ambn)
ACCESSION   NM_009664
VERSION     NM_009664.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2259..2350 
                     /label="Myc-DDK"  
     misc_feature    2380..2965 
                     /label="PolyA signal"  
     misc_feature    2437..2456 
                     /label="Restriction Enzyme"  
     misc_feature    3241..3913 
                     /label="ColE1"  
     misc_feature    4343..5137 
                     /label="Kan/Neo"  
     misc_feature    5172..5474 
                     /label="SV40 ori"  
     misc_feature    5560..6037 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2241..2247 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2241 
                     /label="Ambn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGCATCT AAGATTCCAC TTTTCAAAAT GAAGGGCCTG ATCCTGTTCC 
     1081 TGTCCCTAGT GAAAATGAGC CTCGCCGTGC CGGCATTTCC TCAACAACCT GGGGCTCAAG 
     1141 GCATGGCACC TCCTGGCATG GCTAGTTTGA GCCTTGAGAC AATGAGACAG TTGGGAAGCT 
     1201 TGCAGGGACT CAACGCACTT TCTCAGTATT CTAGACTTGG CTTTGGAAAA GCACTTAATA 
     1261 GTTTATGGTT GCACGGACTT CTCCCACCGC ATAACTCTTT CCCATGGATA GGACCAAGGG 
     1321 AACATGAAAC CCAGCAGCCA TCCTTGCAGC CTCACCAGCC AGGACTGAAA CCCTTCCTCC 
     1381 AGCCCACTGC TGCAACCGGT GTCCAGGTCA CACCCCAGAA GCCAGGGCCT CAGCCTCCAA 
     1441 TGCACCCTGG ACAGCTGCCC TTGCAGGAAG GAGAGCTGAT AGCACCAGAT GAGCCGCAGG 
     1501 TGGCACCATC CGAAAACCCA CCAACACCTG AGGTACCAAT AATGGATTTT GCTGATCCAC 
     1561 AATTTCCAAC CGTGTTCCAG ATCGCCCGTT CAATATCTCG GGGACCAATG GCACACAACA 
     1621 AAGCATCCGC TTTTTACCCA GGAATGTTTT ACATGTCTTA TGGAGCAAAC CAATTGAATG 
     1681 CTCCTGCCAG AATTGGCTTC ATGAGTTCAG AAGAAATGCC TGGAGAAAGA GGAAGTCCCA 
     1741 TGGCCTATGG AACTCTGTTC CCAAGATTTG GAGGCTTCAG GCAAACCCTT AGGAGACTGA 
     1801 ATCAGAATTC ACCCAAGGGA GGAGACTTTA CTGTGGAAGT AGATTCCCCA GTATCTGTTA 
     1861 CCAAAGGCCC TGAAAAAGGA GAAGGTCCAG AAGGCTCTCC ACTGCAAGAG GCCAACCCAG 
     1921 GCAAACGGGA AAACCCCGCT CTCCTTTCAC AAATGGCACC TGGGGCCCAT GCAGGACTTC 
     1981 TTGCTTTCCC CAATGACCAC ATCCCCAGTA TGGCAAGGGG TCCTGCAGGG CAAAGACTCC 
     2041 TTGGAGTCAC CCCTGCAGCT GCAGACCCAC TGATCACCCC TGAATTAGCA GAAGTTTATG 
     2101 AAACCTATGG TGCTGATGTT ACCACACCCC TGGGTGATGG AGAAGCAACC ATGGATATCA 
     2161 CCATGTCCCC AGACACTCAG CAGCCACTGC TACCTGGAAA CAAAGTGCAC CAGCCCCAGG 
     2221 TGCACAACGC ATGGCGTTTC CAAGAGCCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2281 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     2341 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     2401 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     2461 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     2521 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     2581 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     2641 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     2701 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     2761 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     2821 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     2881 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     2941 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3001 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3061 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3121 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3181 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3241 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3301 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     3361 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     3421 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     3481 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     3541 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     3601 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     3661 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     3721 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     3781 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     3841 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     3901 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     3961 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4021 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4081 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4141 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4201 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4261 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4321 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     4381 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     4441 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     4501 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     4561 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     4621 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     4681 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     4741 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     4801 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     4861 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     4921 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     4981 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5041 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5101 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5161 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5221 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5281 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     5341 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     5401 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     5461 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     5521 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     5581 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     5641 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     5701 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     5761 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     5821 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     5881 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     5941 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6001 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6061 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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