LOCUS       MR221862                8853 bp    DNA     circular  SYN 
DEFINITION  Usp42 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 42 (Usp42)
ACCESSION   NM_029749
VERSION     NM_029749.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5010..5101 
                     /label="Myc-DDK"  
     misc_feature    5131..5716 
                     /label="PolyA signal"  
     misc_feature    5188..5207 
                     /label="Restriction Enzyme"  
     misc_feature    5992..6664 
                     /label="ColE1"  
     misc_feature    7094..7888 
                     /label="Kan/Neo"  
     misc_feature    7923..8225 
                     /label="SV40 ori"  
     misc_feature    8311..8788 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4992..4998 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4992 
                     /label="Usp42"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCATAGTT GACAAAACTG AACCTTCAGA CCCATCAACC TGTCAGAACC 
     1081 AGCCTGGCAG TTGTGAGGCG GTCTCACCTG AAGACATGGA CACAGGCTCT GCCAGCTGGG 
     1141 GCGCTGTGTC TTCAATAAGT GATGTCTCAA GTCACACACT TCCATTAGGG CCAGTGCCTG 
     1201 GTGCTGTAGT TTATTCTAAC TCGTCTGTAC CTGAAAAATC AAAGCCATCA CCACCAAAGG 
     1261 ATCAAGTCCT AGGTGATGGC ATTGCTCCTC CTCAAAAGGT CCTGTTTCCA TCTGAAAAGA 
     1321 TTTGTCTTAA GTGGCAACAA AGTCATCGAG TTGGCGCTGG GCTCCAGAAT TTGGGCAACA 
     1381 CCTGTTTTGC CAATGCCGCA TTGCAGTGTC TGACTTACAC GCCACCCCTC GCCAATTACA 
     1441 TGTTATCCCA TGAACACTCC AAGACATGCC ACGCAGAAGG ATTTTGTATG ATGTGCACGA 
     1501 TGCAGACACA CATTACCCAG GCACTTAGCA ACCCTGGGGA TGTTATCAAG CCGATGTTCG 
     1561 TCATCAATGA AATGCGGCGT ATAGCTAGAC ACTTCCGTTT TGGAAACCAA GAAGATGCCC 
     1621 ATGAATTTCT TCAGTACACG GTCGATGCCA TGCAGAAAGC ATGTTTAAAT GGCAGCAATA 
     1681 AATTAGACAG ACACACCCAG GCCACCACCC TGGTCTGCCA GATATTTGGA GGCTACCTAA 
     1741 GATCCCGAGT TAAATGTTTA AATTGCAAGG GTGTTTCAGA TACCTTTGAT CCATATCTGG 
     1801 ACATAACGTT GGAGATTAAG GCTGCACAGA GTGTTACCAA GGCGTTAGAG CAGTTTGTGA 
     1861 AGCCAGAACA ACTGGATGGA GAAAACTCCT ACAAGTGCAG CAAGTGCAAA AAAATGGTTC 
     1921 CAGCTTCAAA GAGATTCACA ATCCATAGGT CCTCTAATGT TCTTACCATC TCACTGAAGC 
     1981 GCTTTGCCAA CTTCACCGGT GGAAAGATTG CTAAGGATGT GAAATATCCT GAGTACCTTG 
     2041 ATATCCGGCC CTATATGTCT CAGCCCAATG GAGAGCCAAT TATTTATGTT TTGTATGCTG 
     2101 TGCTGGTGCA CACTGGTTTT AATTGTCATG CTGGCCACTA CTTTTGCTAC ATCAAGGCTA 
     2161 GCAATGGCCT CTGGTATCAG ATGAATGACT CCATCGTGTC CACCAGTGAT ATCAGAGCAG 
     2221 TGCTTAACCA GCAAGCTTAC GTGCTCTTTT ATATCAGGTC CCATGATGTG AAAAATGGAG 
     2281 GGGAGTCTGC TCATCCTGCC CATAGCCCCG GCCAATCCTC TCCCCGCCCA GGAGTCAGTC 
     2341 AGCGGGTAGT CAACAACAAG CAGGTGGCTC CAGGGTTTAT TGGACCCCAG CTGCCTTCCC 
     2401 ATGTGATGAA GAACACGCCA CACTTGAATG GCACCACGCC AGTGAAAGAC ACACCAAGTA 
     2461 GTTCTGTGTC AAGCCCTAAC GGAAACACCA GCGTCAATAG GGCCAGTCCT GCTACTGCTT 
     2521 CGACTTCTGT GCAGAACTGG TCTGTTACCA GACCCTCAGT TATTCCAGAT CACCCCAAGA 
     2581 AACAAAAAAT CACCATCAGT ATTCACAACA AGTTGCCTGC TCGCCAGGGT CAGGCACCAC 
     2641 TGAATAACAG CCTCCATGGC CCTTGTCTGG AGGCTCCTAG TAAGGCGGCA CCCTCCTCCA 
     2701 CCATCACTAA CCCTTCTGCA ATACAGTCTA CCTCGAACGT ACCCACAACG TCGACTTCCC 
     2761 CCAGTGAGGC CTGTCCCAAG CCCATGGTGA ACGGCAAGGC TAAAGTGGGC GCCAGTGTGC 
     2821 TTGTCCCCTA TGGGGCCGAG TCCTCAGAAG AGTCTGATGA GGAGTCGAAG GGCCTGGCCA 
     2881 AGGAGAACGG TGTGGACATG ATGGCCGGCA CTCACTCCGA TAGGCCAGAA GCTGCTGCAG 
     2941 ATGACGGTGC TGAGGCTTCC TCCCATGAGC TTCAAGAACC CGTCCTGCTA AATGGTGCTA 
     3001 ATAGCGCAGA CAGTGACTCA CAAGAGAACA GCCTGGCATT TGACAGTGCC AGCTGCCAGG 
     3061 TCCAGCCCGA GCTACACACA GAAAACCTCT TTTCCAAACT TAATGGTCTT CCTGGAAAGG 
     3121 TGACGCCTGC TCCTTTGCAG TCTGTTCCTG AAGACAGAAT CCTTGAGACC TTCAAGCTTA 
     3181 CCAACCAGGC AAAGGGTCCA GCGGGTGAAG AGAGTTGGAC TACGACAGGG GGAAGCTCTC 
     3241 CAAAGGACCC TGTTTCACAG CTGGAGCCCA TCAGTGATGA GCCCAGTCCC CTTGAGATAC 
     3301 CGGAGGCTGT CACCAATGGG AGCACACAGA CCCCTTCCAC CACATCACCC CTGGAGCCCA 
     3361 CCATCAGCTG TACCAAAGAA GACTCGTCCG TTGTTGTCTC AGCTGAACCT GTGGAGGGTT 
     3421 TGCCTTCCGT CCCTGCTCTT TGTAACAGCA CTGGTACTAT CTTGGGGGAT ACCCCAGTGC 
     3481 CCGAATTGTG TGACCCTGGA GACTTGACTG CCAACCCGAG CCAGCCAACC GAAGCAGTGA 
     3541 AAGGTGATAC AGCTGAGAAG GCTCAGGACT CTGCCATGGC TGAAGTGGTG GAGAGGCTGA 
     3601 GCCCTGCTCC CTCAGTACTC ACAGGTGACG GGTGTGAGCA GAAACTCTTA CTTTACCTCA 
     3661 GCGCAGAGGG GTCAGAGGAG ACAGAAGACT CTTCCAGAAG CTCGGCGGTC TCTGCTGACA 
     3721 CGATGCCCCC TAAGCCTGAC AGGACCACCA CCAGCTCCTG TGAAGGGGCT GCCGAGCAGG 
     3781 CTGCTGGGGA CAGAGGCGAT GGAGGCCATG TGGGACCCAA AGCTCAGGAG CCTTCCCCAG 
     3841 CCAAGGAAAA GATGAGCAGC CTCCGGAAAG TGGACCGAGG ACACTATCGG AGCCGGAGAG 
     3901 AGCGCTCCTC CAGTGGGGAG CACGTGAGGG ACAGCAGGCC CCGGCCGGAG GACCATCACC 
     3961 ATAAGAAGCG GCACTGCTAC AGCCGAGAGC GGCCCAAGCA GGACCGACAC CCTACTAATT 
     4021 CATACTGCAA TGGGGGCCAG CACTTGGGCC ACGGGGACAG AGCCAGCCCT GAGCGCCGCT 
     4081 CCCTGAGCAG GTATAGTCAC CACCACTCAC GGATTAGGAG TGGCCTGGAG CAGGACTGGA 
     4141 GCCGGTACCA CCATTTGGAA AATGAGCATG CTTGGGTCAG GGAGAGATTC TACCAGGACA 
     4201 AGCTGCGGTG GGACAAGTGC AGGTATTACC ACGACAGGTA CACGCCCCTA TACACGGCCC 
     4261 GGGACGCCCG AGAATGGCGG CCTCTGCATG GTCGTGAGCA TGACCGCCTT GTCCAGTCTG 
     4321 GACGGCCATA CAAGGACAGC TACTGGGGCC GCAAGGGCTG GGAGCTGCAA TCCCGGGGGA 
     4381 AGGAACGGCC CCACTTCAAC AGCCCCCGAG AGGCCCCTAG CCTTGCTGTG CCCCTCGAGA 
     4441 GACATCTCCA AGAGAAGGCT GCGCTGAGTG TGCAGGACAG CAGCCACAGT CTCCCTGAGC 
     4501 GCTTTCATGA ACACAAAAGT GTCAAGTCGA GGAAGCGGAG GTATGAGACT CTAGAAAATA 
     4561 ATGATGGCCG TCTAGAAAAG AAAGTCCACA AAAGCCTGGA GAAGGACACG CTAGAGGAGC 
     4621 CAAGGGTGAA GAAGCACAAA AAGTCTAAAA AGAAAAAGAA GTCCAAAGAT AAACACCGGG 
     4681 ATCGAGAAAG CAGGCACCAG CAGGAGTCTG ATTTTTCAGG AGCATACTCT GATGCTGACC 
     4741 TCCATAGACA CCGGAAGAAA AAGAAGAAAA AGAAAAGGCA TTCCAGGAAG TCGGAGGACT 
     4801 TTATAAAGGA TGTTGAGATG CGTTTACCGA AGCTCTCCAG CTACGAGGCC GGCGGCCATT 
     4861 TCCGGAGAAC AGAGGGCAGC TTTCTCCTGG CTGATGGTCT GCCTGTGGAA GACAGCGGCC 
     4921 CTTTCCGGGA GAAAACGAAG CATTTAAGGA TGGAAAGCCG GCCTGACAGA TGCCGTCTGT 
     4981 CGGAGTATGG CCAGGGTGAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5041 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5101 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5161 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5221 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5281 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5341 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5401 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5461 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5521 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5581 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5641 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5701 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5761 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5821 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5881 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5941 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6001 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6061 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6121 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6181 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6241 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6301 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6361 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6421 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6481 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6541 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6601 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6661 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6721 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6781 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6841 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6901 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6961 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7021 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7081 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7141 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7201 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7261 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7321 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7381 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7441 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7501 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7561 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7621 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7681 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7741 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7801 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7861 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7921 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7981 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8041 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8101 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8221 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8281 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8341 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8401 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8461 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8521 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8581 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8641 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8701 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8761 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8821 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//