LOCUS       MR221858                7995 bp    DNA     circular  SYN 
DEFINITION  Ipo7 (Myc-DDK-tagged) - Mouse importin 7 (Ipo7)
ACCESSION   NM_181517
VERSION     NM_181517.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4152..4243 
                     /label="Myc-DDK"  
     misc_feature    4273..4858 
                     /label="PolyA signal"  
     misc_feature    4330..4349 
                     /label="Restriction Enzyme"  
     misc_feature    5134..5806 
                     /label="ColE1"  
     misc_feature    6236..7030 
                     /label="Kan/Neo"  
     misc_feature    7065..7367 
                     /label="SV40 ori"  
     misc_feature    7453..7930 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4134..4140 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4134 
                     /label="Ipo7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCCAAC ACCATCATCG AGGCCCTCCG GGGCACCATG GACCCAGCCC 
     1081 TGCGCGAGGC CGCTGAGCGC CAGCTCAATG AAGCACACAA GTCTCTGAAT TTCGTTTCAA 
     1141 CACTGCTCCA GATTACTATG TCAGAACAAC TGGATTTACC TGTGAGACAG GCAGGTGTTA 
     1201 TTTATTTAAA AAATATGATA ACACAATATT GGCCTGATCG GGAAGCCACA CCAGGGGACA 
     1261 TCGCTCCATA TACTATTCCA GAAGAAGACC GGCATTGTAT TCGAGAAAAT ATTGTAGAAG 
     1321 CCATTATCCA CTCACCTGAG CTTATCAGGG TACAGCTTAC TACGTGCATC CATCATATCA 
     1381 TCAAACATGA CTATCCAAGC CGCTGGACTG CCATTGTGGA CAAGATTGGC TTTTATCTTC 
     1441 AGTCTGATAA CAGTGCTTGT TGGCTGGGAA TCCTTCTTTG CCTTTATCAG CTTGTGAAAA 
     1501 ATTATGAGTA CAAGAAACCA GAAGAGCGAA GTCCGTTAGT GGCAGCCATG CAGCACTTTC 
     1561 TGCCAGTTCT GAAGGATCGC TTTATCCAGC TGCTTTCTGA CCAATCCGAC CAGTCTGTCC 
     1621 TCATTCAAAA GCAGATATTC AAGATCTTCT ATGCTCTTGT TCAGTATACA CTACCCCTGG 
     1681 AGCTGATAAA CCAACAGAAT CTAACTGAAT GGGTAGAAAT TTTGAAGACA GTTGTGAATA 
     1741 GGGATGTACC TAATGAAACA CTTCAAGTTG AAGAAGATGA TAGACCTGAG TTACCCTGGT 
     1801 GGAAATGTAA GAAGTGGGCC TTACATATTT TAGCAAGACT TTTTGAAAGA TATGGAAGCC 
     1861 CTGGGAATGT TTCTAAGGAG TATAATGAGT TTGCTGAAGT CTTTCTGAAG GCCTTTGCTG 
     1921 TTGGTGTCCA GCAAGTTTTA TTGAAAGTGT TATATCAGTA CAAGGAAAAA CAATATATGG 
     1981 CGCCTCGAGT TTTACAGCAG ACATTAAATT ATATTAATCA AGGAGTTTCC CATGCACTTA 
     2041 CGTGGAAGAA CCTGAAGCCC CATATACAAG GCATTATCCA AGATGTTATT TTTCCATTGA 
     2101 TGTGCTATAC AGATGCTGAT GAGGAGCTCT GGCAAGAAGA TCCTTATGAA TATATACGCA 
     2161 TGAAGTTCGA TGTGTTTGAA GATTTCATTT CTCCTACTAC TGCTGCTCAA ACACTTTTGT 
     2221 TTACAGCCTG TAGCAAAAGG AAAGAGGTAC TGCAAAAGAC TATGGGATTT TGTTACCAAA 
     2281 TTCTTACTGA ACCCAATGCT GATCCTCGGA AAAAAGATGG AGCCTTGCAT ATGATTGGCT 
     2341 CTTTAGCTGA AATACTTCTA AAGAAAAAGA TCTACAAAGA TCAAATGGAA TACATGTTGC 
     2401 AAAATCATGT ATTTCCTCTC TTCAGCAGTG AGCTAGGTTA CATGAGAGCA AGGGCTTGCT 
     2461 GGGTTCTTCA CTATTTTTGT GAAGTGAAGT TCAAAAGTGA TCAAAATCTC CAAACAGCTT 
     2521 TAGAGCTGAC AAGAAGATGT CTGATTGATG ATCGAGAAAT GCCTGTGAAA GTGGAAGCTG 
     2581 CCATTGCACT ACAGGTGTTG ATCAGCAATC AAGAAAAAGC TAAAGAGTAC ATCACTCCAT 
     2641 TTATCAGACC TGTCATGCAG GCTCTTCTTC ATATTATAAG AGAAACAGAA AATGATGATC 
     2701 TTACAAATGT AATTCAGAAA ATGATCTGTG AATATAGTGA AGAAGTTACT CCTATTGCTG 
     2761 TAGAAATGAC ACAACATCTG GCAATGACAT TTAACCAAGT AATCCAGACT GGGCCAGATG 
     2821 AGGAAGGAAG TGATGACAAA GCAGTTACTG CTATGGGAAT TCTGAACACA ATTGATACAC 
     2881 TTCTGAGTGT GGTTGAAGAT CACAAAGAGA TAACCCAGCA GCTTGAGGGA ATCTGCTTAC 
     2941 AAGTCATTGG GACCGTTCTA CAACAGCATG TCTTAGAATT CTATGAGGAG ATCTTTTCTT 
     3001 TGGCACATAG TCTGACATGT CAACAAGTGT CTCCTCAGAT GTGGCAGTTA CTTCCCCTTG 
     3061 TGTTTGAGGT ATTTCAGCAA GATGGCTTTG ATTACTTTAC AGACATGATG CCTCTTCTAC 
     3121 ATAACTATGT AACAGTTGAT ACAGACACTC TTCTCTCTGA TACCAAGTAT CTGGAGATGA 
     3181 TATACAGTAT GTGTAAAAAG GTTCTTACTG GAGTCGCAGG AGAAGATGCG GAATGCCATG 
     3241 CGGCAAAATT GTTAGAGGTC ATTATTCTGC AGTGCAAAGG CCGGGGCATT GATCAGTGTA 
     3301 TACCATTATT CGTGGAAGCA GCTTTAGAAA GACTGACCAG AGAGGTTAAA ACAAGTGAAC 
     3361 TCAGAACAAT GTGCCTGCAG GTTGCAATTG CAGCATTGTA TTATAATCCA CACCTACTGC 
     3421 TTAATACTTT AGAAAATCTT CGCTTCCCAA ATAATGTTGA ACCTGTTACA AATCATTTTA 
     3481 TCACACAATG GCTTAATGAC GTTGACTGTT TTCTGGGGCT TCATGACAGG AAGATGTGTG 
     3541 TTCTTGGCCT ATGCGCCCTT ATTGATATGG AACAAATACC ACAGGTTTTA AATCAGGTTT 
     3601 CAGGACAGAT TTTGCCAGCT TTTATCCTTT TATTTAATGG ATTGAAAAGA GCATATGCCT 
     3661 GCCATGCAGA ACACGAGAAT GACAGTGACG ATGATGAGGA TGCGGAAGAT GATGATGAAA 
     3721 CTGAAGAACT GGGGAGTGAT GAAGATGATA TTGATGAAGA TGGACAAGAA TATTTGGAGA 
     3781 TCCTGGCTAA ACAAGCTGGT GAGGATGGAG ATGATGAAGA TTGGGAAGAA GATGATGCTG 
     3841 AGGAGACTGC TCTGGAAGGC TATTCCACAA TCATCGATGA TGAAGACAAC CCTGTTGATG 
     3901 AGTATCAAAT CTTTAAAGCT ATATTTCAAA CTATTCAAAA TCGCAATCCT GTATGGTATC 
     3961 AGGCTTTGAC TCACGGTCTT AATGAAGAGC AAAGGAAACA GTTACAGGAC ATAGCAACTC 
     4021 TAGCTGATCA AAGAAGAGCA GCCCATGAGT CCAAAATGAT TGAAAAGCAT GGAGGATACA 
     4081 AATTCAGTGC ACCAGTTGTG CCAAGTTCTT TCAATTTTGG AGGCCCAGCA CCGGGGATGA 
     4141 ATACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4201 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4261 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4321 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4381 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4441 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4501 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4561 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4621 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4681 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4741 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4801 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4861 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4921 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4981 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5041 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5641 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5701 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5761 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5821 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5881 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5941 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6001 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6061 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6121 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6181 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6241 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6301 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6361 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6421 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6481 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6541 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6601 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6661 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6721 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6781 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6841 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6901 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6961 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7021 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7081 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7141 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7201 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7321 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7381 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7441 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7501 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7561 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7621 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7681 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7741 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7801 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7861 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7921 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7981 TTTAACGCGA ATTTT
//