LOCUS       MR221799                7047 bp    DNA     circular  SYN 
DEFINITION  Gne (Myc-DDK-tagged) - Mouse glucosamine (Gne), transcript variant 1
ACCESSION   NM_001190414
VERSION     NM_001190414.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3204..3295 
                     /label="Myc-DDK"  
     misc_feature    3325..3910 
                     /label="PolyA signal"  
     misc_feature    3382..3401 
                     /label="Restriction Enzyme"  
     misc_feature    4186..4858 
                     /label="ColE1"  
     misc_feature    5288..6082 
                     /label="Kan/Neo"  
     misc_feature    6117..6419 
                     /label="SV40 ori"  
     misc_feature    6505..6982 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3186..3192 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3186 
                     /label="Gne"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGAAC GGGAACAACC GAAAGCTCCG GGTTTGCGTT GCCACCTGCA 
     1081 ACCGAGCTGA CTACTCCAAA CTGGCCCCGA TCATGTTCGG CATCAAGACA GAGCCCGCGT 
     1141 TCTTTGAGTT GGACGTGGTG GTGCTCGGCT CCCACCTGAT TGACGACTAT GGAAACACAT 
     1201 ACCGCATGAT TGAGCAAGAT GACTTTGACA TTAACACCAG GCTCCACACG ATTGTTAGAG 
     1261 GGGAAGATGA AGCGGCCATG GTAGAGTCGG TAGGCCTAGC GCTCGTGAAG CTACCGGACG 
     1321 TCCTCAATCG CCTGAAGCCC GACATCATGA TTGTTCACGG AGACCGATTT GACGCCCTTG 
     1381 CTCTGGCTAC GTCTGCTGCC TTGATGAACA TCCGCATCCT TCACATTGAA GGAGGCGAGG 
     1441 TCAGCGGGAC CATTGATGAC TCTATCAGAC ACGCCATAAC AAAACTGGCT CACTACCATG 
     1501 TGTGCTGCAC TAGAAGTGCA GAGCAGCACC TGATCTCTAT GTGCGAGGAC CACGACCGCA 
     1561 TCCTGTTGGC AGGCTGCCCT TCCTATGACA AACTGCTCTC CGCCAAGAAC AAAGACTATA 
     1621 TGAGCATCAT TCGGATGTGG CTAGGCGATG ATGTAAAATG TAAGGATTAC ATCGTTGCCC 
     1681 TGCAGCATCC CGTGACCACT GACATTAAGC ATTCCATAAA GATGTTTGAG CTAACACTGG 
     1741 ATGCCCTGAT CTCGTTTAAC AAGAGGACCC TAGTTCTGTT TCCAAATATC GATGCAGGCA 
     1801 GCAAGGAGAT GGTTCGAGTG ATGCGGAAGA AGGGCATCGA GCATCACCCC AATTTCCGTG 
     1861 CAGTCAAGCA CGTCCCGTTT GACCAGTTCA TACAGCTGGT CGCCCACGCT GGCTGCATGA 
     1921 TTGGGAATAG CAGCTGCGGC GTGCGAGAGG TTGGCGCTTT CGGAACACCC GTGATCAACC 
     1981 TGGGCACAAG GCAGATAGGA AGAGAAACCG GGGAGAATGT TCTTCATGTC AGGGATGCTG 
     2041 ACACCCAAGA TAAAATATTG CAAGCACTAC ACCTCCAGTT CGGCAAACAG TACCCTTGCT 
     2101 CAAAGATATA TGGGGATGGG AATGCTGTTC CAAGGATTTT AAAGTTTCTC AAATCCATTG 
     2161 ACCTTCAAGA GCCACTACAG AAGAAATTCT GCTTCCCCCC TGTAAAGGAG AACATCTCTC 
     2221 AAGACATTGA CCACATCCTG GAAACTCTGA GTGCCTTGGC TGTTGATCTT GGCGGGACAA 
     2281 ACCTGAGGGT GGCAATAGTT AGCATGAAGG GTGAAATCGT TAAGAAGTAC ACTCAGTTCA 
     2341 ACCCTAAAAC CTATGAAGAA AGGATTAGTT TAATCCTGCA GATGTGTGTG GAAGCTGCCG 
     2401 CGGAAGCTGT GAAACTCAAT TGCAGAATTC TGGGAGTAGG CATCTCCACA GGTGGCCGCG 
     2461 TGAATCCCCA GGAAGGAGTT GTGCTGCATT CAACCAAGCT GATCCAGGAA TGGAACTCCG 
     2521 TGGACCTCAG GACACCCCTC TCCGACACCC TGCATCTCCC CGTGTGGGTG GACAATGACG 
     2581 GCAACTGTGC CGCCATGGCA GAGAGGAAGT TCGGCCAAGG AAAAGGACAG GAGAACTTCG 
     2641 TGACGCTCAT CACGGGGACA GGGATCGGTG GGGGGATCAT CCACCAGCAC GAACTGATCC 
     2701 ACGGCAGCTC CTTCTGCGCG GCGGAGCTCG GCCATCTCGT GGTGTCCCTG GACGGTCCTG 
     2761 ACTGCTCCTG TGGAAGCCAT GGGTGCATCG AAGCGTACGC CTCTGGAATG GCCTTGCAGA 
     2821 GGGAAGCAAA GAAACTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG TCAGTACCAA 
     2881 AAGACGAAGC TGTGGGTGCC CTCCATCTCA TCCAGGCTGC CAAGCTGGGC AACGTGAAGG 
     2941 CCCAGAGCAT CTTACGAACA GCTGGAACTG CTTTGGGACT TGGGGTTGTG AACATCCTCC 
     3001 ACACTATGAA TCCTTCCCTG GTGATCCTGT CTGGAGTCCT GGCCAGTCAC TACATCCACA 
     3061 TCGTGAAGGA CGTCATCCGC CAGCAAGCCT TGTCCTCCGT GCAGGATGTG GACGTGGTGG 
     3121 TCTCAGACTT GGTGGACCCG GCCCTGCTTG GCGCAGCCAG CATGGTTCTG GACTACACAA 
     3181 CGCGCAGGAT CCACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3241 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3301 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3361 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3421 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3481 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3541 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3601 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3661 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3721 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3781 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3841 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3901 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3961 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4021 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4081 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4141 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4201 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4261 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4321 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4381 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4441 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4501 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4561 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4621 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4681 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4741 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4801 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4861 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4921 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4981 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5041 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5101 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5161 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5221 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5281 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5341 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5401 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5461 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5521 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5581 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5641 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5701 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5761 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5821 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5881 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5941 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6001 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6061 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6121 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6181 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6241 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6301 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6361 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6421 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6481 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6541 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6601 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6661 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6721 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6781 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6841 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6901 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6961 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7021 ATTTAACAAA AATTTAACGC GAATTTT
//