LOCUS       MR221778                9252 bp    DNA     circular  SYN 
DEFINITION  Ptprk (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, K (Ptprk)
ACCESSION   NM_008983
VERSION     NM_008983.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5409..5500 
                     /label="Myc-DDK"  
     misc_feature    5530..6115 
                     /label="PolyA signal"  
     misc_feature    5587..5606 
                     /label="Restriction Enzyme"  
     misc_feature    6391..7063 
                     /label="ColE1"  
     misc_feature    7493..8287 
                     /label="Kan/Neo"  
     misc_feature    8322..8624 
                     /label="SV40 ori"  
     misc_feature    8710..9187 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5391..5397 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5391 
                     /label="Ptprk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTGGCG GCCGCTGCGT TGCCTGCTTT TGTAGCTCTC TGGCTTCTGT 
     1081 ACCCGTGGCC TCTCCTGGGG TCGGCCCTTG GCCAGTTCTC AGCAGGTGGC TGTACTTTTG 
     1141 ATGATGGGCC AGGGGCTTGT GACTACCACC AGGATTTATA CGATGACTTT GAGTGGGTCC 
     1201 ATGTCAGTGC GCAGGAACCT CATTACCTGC CCCCCGAAAT GCCTCAAGGT TCCTATATGG 
     1261 TTGTGGACTC CTCAAATCAT GATCCTGGAG AAAAAGCCAG ACTTCAGCTG CCTACCATGA 
     1321 AGGAGAATGA CACCCACTGC ATTGATTTCA GTTACCTGTT ATATAGCCAG AAGGGGTTGA 
     1381 ACCCTGGCAC TTTGAATATC CTAGTTAGGG TGAATAAAGG ACCTCTTGCT AATCCAATTT 
     1441 GGAATGTAAC TGGATTCACT GGTCGTGATT GGCTTCGGGC TGAACTAGCT GTGAGCACCT 
     1501 TTTGGCCCAA TGAATACCAG GTAATATTTG AAGCTGAAGT CTCAGGAGGG AGAAGTGGTT 
     1561 ATATTGCCAT TGATGACATC CAAGTCCTGA GTTATCCTTG CGATAAATCT CCTCATTTTC 
     1621 TCCGCCTTGG TGATGTGGAG GTCAATGCTG GGCAGAATGC TACATTTCAG TGCATTGCTA 
     1681 CAGGGAGAGA TGCTGTGCAT AACAAGTTAT GGCTGCAGAG ACGCAATGGA GAAGACATAC 
     1741 CCGTAGCCCA GACTAAGAAC ATAAATCACA GAAGATTTGC TGCCTCTTTC AGATTGCAAG 
     1801 AAGTGACAAA AACTGACCAG GATTTGTACC GCTGCGTAAC TCAGTCAGAA CGAGGTTCTG 
     1861 GGGTTTCCAA TTTTGCTCAA CTCATTGTGA GAGAACCACC TAGACCCATT GCTCCTCCCC 
     1921 AGCTGCTTGG TGTTGGGCCT ACTTACTTGC TGATCCAACT AAATGCCAAC TCTATTATTG 
     1981 GCGATGGCCC CATCATCCTG AAAGAAGTAG AGTATCGAAT GACATCAGGA TCTTGGACAG 
     2041 AAACCCATGC AGTCAACGCA CCAACATATA AGTTGTGGCA TTTAGACCCA GATACAGAAT 
     2101 ACGAGATCCG CGTCCTGCTT ACCAGACCTG GCGAAGGGGG AACTGGGCTG CCAGGACCAC 
     2161 CACTGATCAC TAGAACGAAG TGTGCAGAAC CTATGCGGAC ACCAAAGACT TTAAAGATTG 
     2221 CTGAAATCCA GGCAAGGCGC ATTGCAGTGG ACTGGGAGTC CTTGGGCTAC AACATCACTC 
     2281 GTTGCCACAC TTTCAACGTC ACTATCTGCT ACCATTACTT CCGTGGCCAC AATGAGAGCA 
     2341 GGGCAGACTG CTTGGACATG GACCCCAAAG CCCCTCAGCA TGTTGTGAAC CATCTGCCAC 
     2401 CTTACACAAA TGTCAGCCTC AAGATGATCC TAACCAACCC AGAGGGAAGG AAGGAGAGCG 
     2461 AAGAGACAAT CATCCAAACT GATGAAGATG TGCCCGGGCC TGTGCCAGTC AAATCCCTCC 
     2521 AAGGAACATC CTTTGAAAAC AAGATCTTCC TGAACTGGAA AGAGCCACTG GAACCGAATG 
     2581 GAATTATCAC TCAGTATGAG GTGAGCTATA GCAGCATAAG ATCATTTGAC CCTGCTGTTC 
     2641 CAGTGGCTGG GCCCCCACAG ACTGTATCAA ATTTATGGAA TAGTACACAC CATGTATTTA 
     2701 TGCATCTTCA CCCTGGAACC ACCTACCAGT TTTTTATAAG AGCCAGCACT GTCAAAGGCT 
     2761 TTGGACCAGC AACAGCCATC AATGTGACCA CAAATATCTC AGCTCCAAGC TTACCTGACT 
     2821 ATGAAGGAGT TGATGCCTCT CTGAATGAAA CTGCCACCAC CATCACAGTA CTATTGAGGC 
     2881 CTGCACAAGC CAAAGGTGCT CCTATCAGTG CTTATCAAAT TGTTGTGGAG CAGCTACACC 
     2941 CACATCGAAC GAAGCGTGAA GCAGGGGCCA TGGAATGCTA CCAGGTACCG GTTACATACC 
     3001 AGAACGCCCT AAGTGGGGGC GCGCCCTATT ACTTTGCCGC AGAACTTCCC CCTGGGAATC 
     3061 TTCCCGAGCC TGCTCCCTTC ACCGTGGGTG ACAACCGGAC CTATAAAGGC TTTTGGAACC 
     3121 CTCCCCTGGC CCCCCGCAAA GGATACAACA TCTATTTCCA AGCGATGAGC AGTGTGGAGA 
     3181 AGGAAACTAA AACCCAATGT GTACGAATTG CTACAAAAGC AGCAGCAACA GAAGAACCAG 
     3241 AAGTGATCCC AGACCCGGCA AAGCAGACAG ACAGAGTGGT GAAAATCGCG GGCATCAGTG 
     3301 CTGGCATCCT AGTGTTCATC CTTCTCCTGC TGGTTGTCAT AGTAATTGTG AAAAAGAGCA 
     3361 AGCTTGCTAA GAAGCGCAAA GATGCAATGG GGAACACACG TCAGGAGATG ACCCACATGG 
     3421 TGAATGCTAT GGACCGAAGT TATGCTGACC AGAGCACCCT GCATGCAGAA GACCCCCTTT 
     3481 CCCTCACCTT CATGGACCAA CATAACTTCA GTCCAAGATT GCCCAATGAT CCACTTGTGC 
     3541 CGACTGCCGT GTTAGATGAG AACCACAGTG CCACAGCAGA GTCTAGTCGT CTCCTGGATG 
     3601 TTCCTCGATA CCTCTGTGAA GGGACAGAGT CCCCTTATCA GACAGGACAG CTGCACCCAG 
     3661 CCATCAGGGT GGCCGACTTA CTGCAGCACA TTAACCTCAT GAAGACATCA GACAGCTATG 
     3721 GGTTCAAAGA GGAATACGAG AGCTTCTTTG AAGGCCAGTC AGCCTCTTGG GATGTGGCTA 
     3781 AAAAGGATCA AAACAGAGCA AAGAACCGAT ACGGAAACAT TATCGCATAT GATCACTCCA 
     3841 GAGTCATCCT GCAACCTGTG GAAGATGACC CTTCTTCAGA TTACATTAAT GCCAACTACA 
     3901 TCGACATTTG GCTGTACAGG GATGGCTACC AGAGACCAAG CCACTACATT GCAACTCAAG 
     3961 GCCCAGTTCA TGAAACCGTA TATGATTTTT GGAGGATGGT GTGGCAAGAG CAGTCTGCCT 
     4021 GTATTGTGAT GGTCACTAAT TTAGTGGAAG TTGGCCGGGT GAAATGCTAT AAATATTGGC 
     4081 CTGATGATAC TGAGGTTTAT GGTGACTTCA AAGTCACCTG CGTAGAAATG GAGCCACTTG 
     4141 CTGAGTATGT CGTTAGGACA TTCACCTTGG AAAGGAGGGG CTATAATGAA ATCCGTGAAG 
     4201 TCAAACAGTT CCACTTCACT GGCTGGCCTG ACCATGGTGT TCCATACCAC GCAACAGGGC 
     4261 TCCTGTCATT TATCCGGAGA GTCAAGCTAT CTAACCCTCC CAGTGCTGGG CCCATTGTCG 
     4321 TACACTGCAG TGCTGGTGCT GGGCGCACAG GCTGTTACAT TGTTATTGAC ATAATGCTGG 
     4381 ACATGGCTGA AAGAGAGGGT GTGGTTGACA TCTACAACTG TGTGAAAGCC TTACGATCTC 
     4441 GGCGCATTAA TATGGTACAG ACAGAGGAAC AGTACATTTT TATTCATGAT GCCATTTTAG 
     4501 AAGCCTGCTT ATGTGGAGAA ACTGCCATCC CTGTGTGTGA ATTTAAAGCT GCATATTTTG 
     4561 ATATGATTCG AATAGACTCT CAGACTAACT CCTCTCATCT CAAAGATGAA TTTCAGACTC 
     4621 TGAATTCGGT CACCCCTCGA CTACAAGCTG AAGACTGCAG CATAGCCTGC CTGCCAAGGA 
     4681 ACCATGACAA GAACCGTTTC ATGGATATGC TCCCACCTGA CAGATGTCTG CCTTTTTTAA 
     4741 TTACAATTGA TGGGGAGAGC AGTAACTACA TCAATGCTGC TCTTATGGAT AGCTATAGGC 
     4801 AGCCAGCAGC TTTCATCGTC ACACAATACC CACTGCCAAA CACTGTGAAA GACTTCTGGA 
     4861 GATTAGTATA TGATTACGGA TGTACCTCCA TCGTGATGCT AAATGAAGTG GACCTGTCTC 
     4921 AGGGCTGCCC ACAGTACTGG CCAGAAGAAG GAATGCTGCG ATATGGTCCT ATCCAAGTGG 
     4981 AATGTATGTC TTGTTCAATG GACTGTGATG TGATCAATCG AATTTTTAGA ATATGCAACC 
     5041 TAACGAGACC ACAGGAGGGC TATCTGATGG TACAACAGTT CCAGTACCTA GGCTGGGCTT 
     5101 CTCATCGAGA AGTGCCTGGC TCCAAACGCT CGTTTTTGAA ATTGATACTG CAGGTGGAAA 
     5161 AATGGCAAGA GGAATGTGAA GAAGGGGAAG GCCGGACAAT CATCCACTGC TTGAATGGCG 
     5221 GTGGGCGCAG TGGCATGTTC TGTGCCATAG GCATTGTTGT GGAGATGGTG AAGCGGCAAA 
     5281 ATGTGGTGGA TGTTTTCCAT GCAGTAAAGA CGCTGAGGAA CAGCAAGCCA AACATGGTGG 
     5341 AAGCCCCGGA GCAGTATCGT TTTTGCTATG ATGTGGCGTT AGAGTACCTG GAGTCCTCAA 
     5401 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5461 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5521 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5581 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5641 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5701 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5761 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5821 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5881 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5941 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6001 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6061 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6121 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6181 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6241 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6301 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6361 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6421 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6481 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6541 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6601 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6661 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6721 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6781 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6841 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6901 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6961 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7021 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7081 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7141 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7201 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7261 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7321 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7381 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7441 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7501 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7561 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7621 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7681 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7741 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7801 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7861 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7921 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7981 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8041 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8101 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8161 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8221 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8281 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8341 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8401 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8461 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8521 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8581 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8641 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8701 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8761 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8821 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8881 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8941 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9001 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9061 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9121 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9181 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9241 AACGCGAATT TT
//