LOCUS       MR221777                8931 bp    DNA     circular  SYN 
DEFINITION  Ptprj (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1
ACCESSION   NM_008982
VERSION     NM_008982.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5088..5179 
                     /label="Myc-DDK"  
     misc_feature    5209..5794 
                     /label="PolyA signal"  
     misc_feature    5266..5285 
                     /label="Restriction Enzyme"  
     misc_feature    6070..6742 
                     /label="ColE1"  
     misc_feature    7172..7966 
                     /label="Kan/Neo"  
     misc_feature    8001..8303 
                     /label="SV40 ori"  
     misc_feature    8389..8866 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5070..5076 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5070 
                     /label="Ptprj"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGCGCGGA GGAGGCAGAG GGAGCAGCCG GGGCCGCGGG AGCCGGGAGC 
     1081 TGGGAGCCAC GCGCGGAGGG TGGGCGCCGC TCGCTCCGCC CCGCGAAGCC CCTGCGAGTC 
     1141 TAAGGCCGCG GCCGCTCCGC GCCAGGCGCG CTAGGCTCCG CCGTGTGGCC GCCGCCGCCG 
     1201 CCGCTGCCAT GTCCCCGGGG AAGCCCGGGG CGGGCGGAGC GGGGACTAGG CGGACCGGCT 
     1261 GGCGGAGAAG GAGGCGGAGG CGTCGGCTGG AGACGGAGAC GAGGGCGCCC GGCTTCGGGC 
     1321 ACACGGCGGG GCGCGTCCCG GGCACGTTCC AGGGCGCGCA GGGCATGAAG CCCGCGGCGC 
     1381 GGGAGACGCG GACACCCCCG CGCTCGCCCG GGCTCCGCTG GGCGCTGCTG CCGCTGCTGC 
     1441 TGTTGCTACG CCAGGGCCAG GTCCTGTGCG CAGGTGCGGC ACCGAATCCT ATATTTGACA 
     1501 TTGAAGCTGT CGTCAGCCCA ACTAGTGTGT TATTAACATG GAAGCACAAT GACTCAGGCG 
     1561 CTTCAGAATG TAGAATAGAG AATAAGATGG AGAGCAATCT GACGTTTCCT GTTAAAAACC 
     1621 AGACATCATG TAACATTACA GGCTTAAGCC CAGGCACTTC GTATACATTC TCCATCATCT 
     1681 CTGTAACAAC CAATGAGACC TTGAACAAAA CTATCACAAC AGAGCCCTGG CCAGTGTCTG 
     1741 ATCTCCATGT CACCTCTGTG GGTGTGACAC AGGCTCGTCT CACCTGGAGC AATGCAAATG 
     1801 GCACTGCCTC CTACCGGATG CTGATTGAAG AGTTGACCAC ACATTCCTCA GTCAATATTT 
     1861 CAGGTCTGAA GCCGGGGACC AATAATAGCT TCGCTTTCCC AGAATCAAAT GAGACACAGG 
     1921 CTGACTTTGC AGTTGCAGAG GAGGTCCCGG ATGCCAATGG TACCAAGAGA ATCCCAGTGA 
     1981 CCAACCTATC CCAACTACAC AAGAATTCTC TTGTCTCTGT GGACCCACCC TCTGGCCAGG 
     2041 ATCCCTCCCT CACAGAGATC TTGCTTACTG ACCTAAAGCC TGATACTCAG TACAATGCCA 
     2101 CCATCTATTC TCAAGCAGCA AATGGCACTG AAGGACAGCC CAGGAACAAA GTGTTTAAAA 
     2161 CAAATTCCAC CCAGGTTTCT GACGTCCGAG CTATGAACAT CAGTGCCTCA AGCATGACCC 
     2221 TGACCTGGAA AAGCAATTAC GATGGGTCCC GTACTTCAAT TGTCTACAAA ATACACGTGG 
     2281 CTGGGGGGAC CCACTCCGTC AACCAAACTG TCAATAAGAC TGAGGCCATC ATCCTCGGAC 
     2341 TCAGCTCCAG CACCTTGTAC AACATCACAG TTCATCCTTT CCTGGGTCAG ACGGAGGGCA 
     2401 CACCAGGCTT CCTCCAAGTG TACACTTCCC CCGATCAGGT CTCTGACTTC CGAGTGACAA 
     2461 ATGTCAGCAC AAGGGCAATT GGTTTGGCTT GGAGGAGCAA TGACTCCAAG TCCTTCGAGA 
     2521 TTTTCATCAA GCAGGACGGA GGTGAGAAGC ATCGAAATGC TTCGACGGGA AACCAGAGCT 
     2581 ATATGGTTGA AGATTTAAAG CCTGGAACCA GTTACCATTT TGAGATAATT CCACGAGGAC 
     2641 CAGACGGGAC AGAAGGGCTG TCCAGTACAG TGAATGGGAG CACTGACCCC AGTGCTGTGA 
     2701 CTGACATCCG GGTGGTCAAC ATTAGCACCA CTGAAATGCA GTTGGAGTGG CAGAATACGG 
     2761 ACGATGCCTC TGGATACACT TACCATTTAG TTCTAGAGTC TAAAAGTGGC TCCATCATCA 
     2821 GGACCAACAG TTCTCAGAAG TGGATCACAG TAGGGAGCCT CACCCCAGGC ACCTTATACA 
     2881 ATGTCACAAT CTTTCCAGAA GTGGACCAGA TCCAGGGAAT CTCCAACTCC ATTACCCAGT 
     2941 ACACACGGCC CAGCAGTGTG TCCCACATTG AAGTAAACAC CACCACCACC ACGGCAGCCA 
     3001 TCCGATGGAA GAACGAGGAC GCAGCCTCTG CTTCCTATGC CTACTCCGTC CTTATCTTGA 
     3061 AGACTGGAGA TGGCAGCAAT GTAACCAGCA ACTTCACAAA AGACCCTTCT ATTCTAATCC 
     3121 CTGAGTTAAT CCCTGGTGTC TCTTACACAG TGAAGATCCT TACACAAGTT GGGGATGGTA 
     3181 CAACATCACT GGTACCTGGT TGGAATCTGT TCTGTACGGA ACCTGAACCA GTGACCTCCT 
     3241 TCCACTGTGA AGTGGTCCCT AAGGAGCCAG CATTGGTTCT CAAGTGGGCC TGCCCCTTTG 
     3301 GCATGTACAC AGGCTTCGAG CTGGGGGTCA GGAGTGATTC CTGGGACAAT ATGACACGCC 
     3361 TAGAGAACTG CACTTCGGAT GATGACACAG AGTGCAGGAC GGAAGTCGCC TATTTGAATT 
     3421 TTTCTACCTC GTACAACATC AGCATCGCCA CCTTGTCATG TGGGAAGATG GCGCTTCCCG 
     3481 CCCAGAACAT CTGCACCACT GGCATCACAG ACCCACCTAC TCCGGATGGA TCCCCTAATA 
     3541 TTACATCGGT CAGTCACAAT TCAGTAAAGG TTAAGTTCAG CGGGTTTGAA GCCAGCCACG 
     3601 GACCTATCAA AGCCTATGCT GTCATCCTCA CCACCGGGGA AGCTGCCCAA CCTTCTGCAG 
     3661 ATGTTTTGAA GTACACGTAT GAGGATTTCA AAAGGGGAGC CTCGGATACT TATGTCACAT 
     3721 ACCTCATAAG AATAGAAGAG AAGGGACAGT CTCAGGGCTT GTCTGAAGTC TTGAACTATG 
     3781 AAATTGATGT GGGGAACCAA TCCACTACCC TCGGCTACTA CAACGGGAGG CTGGAGCCTC 
     3841 TGGGCTCCTA CCGGGCTTGT GTTGCTGGCT TTACCAATAT TACCTACAAC CTTCAGAATG 
     3901 ACGGCCTCAT CAATGGGGAT GAGAGCTATG TGTCTTTCAG TCCATATTCA GAGGCCGTGT 
     3961 TCTTGCCCCA GGACCCAGGT GTCATCTGCG GAGCAGTGTT TGGATGTATC TTTGGTGCCC 
     4021 TGGCCATCAC AGCTGTGGGA GGCTTCATCT TCTGGAGAAA GAAAAGGACA GATGCCAAGA 
     4081 ATAATGAAGT GTCCTTTTCT CAAATTAAAC CTAAAAAATC CAAGTTAATC CGAGTGGAGA 
     4141 ATTTTGAGGC CTACTTTAAG AAGCAGCAAG CTGACTCTAA CTGTGGGTTT GCAGAGGAAT 
     4201 ATGAGGACCT GAAGCTGATT GGGATAAGTT TACCTAAATA CACAGCTGAG ATAGCCGAGA 
     4261 ACAGAGGGAA GAACCGCTAC AACAATGTTC TGCCCTATGA TATTTCTCGA GTCAAACTTT 
     4321 CAGTCCAGAC CCATTCGACA GATGACTACA TCAATGCCAA CTATATGCCT GGCTACCATT 
     4381 CCAAGAAAGA TTTCATTGCC ACACAAGGAC CTTTACCCAA CACTTTGAAA GATTTCTGGC 
     4441 GTATGGTTTG GGAGAAAAAC GTATATGCCA TTGTTATGTT GACCAAATGC GTGGAGCAGG 
     4501 GAAGGACCAA ATGTGAGGAG TACTGGCCTT CCAAGCAGGC TCAGGACTAC GGGGACATAA 
     4561 CTGTGGCGAT GACATCAGAA GTCGTTCTTC CAGAATGGAC CATCAGAGAT TTTGTGGTGA 
     4621 AAAATATGCA GAATAGCGAG AGCCATCCTC TGCGGCAGTT CCATTTCACC TCCTGGCCTG 
     4681 ACCACGGTGT TCCTGACACC ACTGACCTGC TCATCAACTT TCGGTACCTG GTCCGGGATT 
     4741 ACATGAAGCA GATACCCCCC GAGTCACCAA TTCTGGTGCA TTGCAGTGCT GGGGTTGGAA 
     4801 GGACGGGCAC TTTCATCGCC ATCGATCGCC TGATCTATCA GATAGAGAAT GAGAACACCG 
     4861 TGGACGTGTA TGGGATTGTC TATGACCTTC GGATGCACAG GCCTCTGATG GTGCAGACAG 
     4921 AGGACCAGTA TGTTTTCCTC AATCAGTGTG TTTTGGATAT TATCAGAGCC CAGAAAGACT 
     4981 CAAAAGTTGA TCTCATCTAT CAGAACACAA CGGCAATGAC AATCTATGAA AACCTCGAGC 
     5041 CAGTGAGCAT GTTTGGAAAG ACTAATGGTT ACATCGCCAC GCGTACGCGG CCGCTCGAGC 
     5101 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5161 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5221 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5281 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5341 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5401 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5461 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5521 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5581 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5641 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5701 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5761 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5821 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5881 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5941 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6001 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6061 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6121 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6181 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6241 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6301 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6361 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6421 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6481 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6541 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6601 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6661 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6721 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6781 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6841 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6901 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6961 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7021 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7081 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7141 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7201 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7261 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7321 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7381 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7441 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7501 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7561 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7621 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7681 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7741 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7801 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7861 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7921 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7981 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8041 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8101 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8161 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8221 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8281 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8341 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8401 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8461 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8521 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8581 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8641 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8701 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8761 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8821 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8881 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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