LOCUS       MR221686                7272 bp    DNA     circular  SYN 
DEFINITION  Casd1 (Myc-DDK-tagged) - Mouse CAS1 domain containing 1 (Casd1)
ACCESSION   NM_145398
VERSION     NM_145398.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3429..3520 
                     /label="Myc-DDK"  
     misc_feature    3550..4135 
                     /label="PolyA signal"  
     misc_feature    3607..3626 
                     /label="Restriction Enzyme"  
     misc_feature    4411..5083 
                     /label="ColE1"  
     misc_feature    5513..6307 
                     /label="Kan/Neo"  
     misc_feature    6342..6644 
                     /label="SV40 ori"  
     misc_feature    6730..7207 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3411..3417 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3411 
                     /label="Casd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCTCTG GCCTACAACC TGGGCAAGCG GGAGATCAAC CACTACTTCA 
     1081 GCGTGAGGAG CGCCAAGGTG CTGGCGCTGG TGGCCGTGCT GCTGCTCGCA GCGTGCCACC 
     1141 TCGCGTCCCG CCGCTACCGA GGCAATGATT CGTGTGAATA CCTTCTCTCG AGCGGCAGAT 
     1201 TTCTTGGAGA AAAAGTTTGG CAACCTCACA GTTGTATGAT GCATAAATAC AAAATAAGTG 
     1261 AAGCAAAGAC TTGCCTTGTA GATAAACACA TTGCGTTTAT TGGAGATTCC AGGATCCGTC 
     1321 AATTATTTTA TTCTTTTGTA AAAATCATTA ACCCCCAGTT TAAAGAAGAA GGAAATAAGC 
     1381 ATGAAAACAT TCCTTTTGAA GACAAGGCTG CATCAGTTAA AGTGGATTTT CTTTGGCATC 
     1441 CAGAAGTTAA TGGTTCCATG AAACAATGTA TCAAGGTGTG GACTGAGGAC TCGGTTTTGA 
     1501 AGCCCCATGT GATTGTAGCA GGAGCAGCCA CATGGTCCAT CAAGATTCAT AATGGCAGCG 
     1561 AGGAGGCACT AGCTCAGTAT AAAATGAATA TCACCTCCAT AGCACCACTC TTAGAAAAAT 
     1621 TGGCCAAGAC TAGTGATGTT TATTGGGTCT TACAAGACCC AGTTTATGAG GATCTCTTAA 
     1681 GTGAAAATAG GAAAATGATT ACCAATGAGA AGATAGACGC CTACAATGAG GCTGCAGTCA 
     1741 GCATCCTGAA CAGCAGCACC AGGACCTCTA AGTCCAATGT GAAGATGTTC AGTGTTTCCA 
     1801 AACTCATTGC CCAAGAGACC ATCATGGAGT CTCTGGATGG CTTACACCTT CCTGAATCGA 
     1861 GCAGAGAAAC TAGTGCAATG ATTCTCATGA ACGTGTACTG CAATAAAGTT GTGAAGCCTG 
     1921 TGGATGGTTC CTGTTGTCAG CCTCGGCCAC CTCTCACTCT CATTCAGAAG CTAGCTGCTT 
     1981 GCTTTTTCAC TTTATCCATT ATTGGGTATT TCATTTTCTA TGTAATTCAT CGTAATGCTC 
     2041 ACCGGAAGAA TAAACCATGT ACTGATTTGG AGAGTGGCGA GGAAAAGAAG AATATTATCA 
     2101 ATACTCCTGT GTCTTCATTA GAAATACTTT TACAGTCTTT TTGCAAACTT GGCCTGATTA 
     2161 TGGCTTATTT CTATATGTGT GACCGTGCAA ACCTGTTTAT GAAGGAAAAC AAATTTTATA 
     2221 CACATTCATC ATTCTTTATT CCAATTATCT ACATCTTGGT TTTGGGAGTA TTTTACAATG 
     2281 AAAACACAAA AGAGACTAAA GTGTTAAATA GAGAGCAAAC TGATGAATGG AAAGGCTGGA 
     2341 TGCAGCTTGT GATTTTGATC TATCACATCT CGGGAGCAAG CACATTTCTG CCTGTGTACA 
     2401 TGCACATTCG AGTTCTGGTG GCTGCATATC TGTTTCAGAC AGGGTACGGG CATTTCTCTT 
     2461 ACTTTTGGAT CAAAGGAGAT TTTGGGATTC ATAGAGTGTG TCAGGTCTTA TTTCGTCTCA 
     2521 ATTTCCTGGT TGTGGTGTTA TGTATCGTAA TGGATCGACC ATATCAGTTC TATTACTTTG 
     2581 TCCCCTTGGT TACCGTGTGG TTCATGGTCA TATATGTCAC TTTAGCACTG TGGCCACAAA 
     2641 TAACCCAAAA GAAGGCAAAT GGAAATTTTT TCTGGTATCT CGGCTTACTG TTGAAACTAG 
     2701 GCTTGCTGCT GCTGTGCATA TGGTTCTTGG CATATTCTCA GGGTGCATTT GAGAAGATCT 
     2761 TTTCTCTATG GCCACTTTCC AAATGTTTTG AACTAGAAGG GAGCGTGTAT GAATGGTGGT 
     2821 TCAGGTGGAG GCTAGACCGC TACGTTGTCT TCCATGGGGT TCTGTTTGCT TTCATTTATC 
     2881 TGGCTTTGCA GAGACGGCAA ATACTTTCTG AAGGAAAGGG TGAACCACTC TTTTCAAACA 
     2941 AAATTTCCAA CTTTCTATTG TTTGTTTCAG TAGTGTCTTT CTTGACCTAT TCCATCTGGG 
     3001 CTAGCAGCTG TAAGAACAAA GCAGAGTGTA ACGAACTCCA CCCATCTGTG TCTGTGGTAC 
     3061 AGATTGTAGC CTTCATCCTG ATAAGGAACA TACCTGGATA TGCCCGTTCT ATATACAGTT 
     3121 CTTTTTTTGC TTGGTTTGGA AAAATTTCAC TAGAGCTGTT CATCTGCCAG TACCACATCT 
     3181 GGCTGGCAGC AGACACAAGG GGCATCCTGG TCCTCATCCC TGGAAACCCG ACACTCAACA 
     3241 TCATCGTCAG CACGTTCATC TTCGTTTGTG TGGCACATGA GATTTCTCAG ATCACCACTG 
     3301 ACCTCGCGCA GGTCGTTATT CCTAAAGATA ACCCGTCTCT CTTCCGAAGG CTGGCATGTA 
     3361 CGATTGCATT CTTTGGTGGA GTCCTCATCT TATCCTCCAT TCAAGATAAA TCAAGACTGA 
     3421 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3481 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3541 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3601 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3661 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3721 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3781 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3841 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3901 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3961 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4021 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4081 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4141 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4201 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4261 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4321 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4381 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4441 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4501 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4561 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4621 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4681 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4741 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4801 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4861 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4921 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4981 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5041 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5101 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5161 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5221 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5281 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5341 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5401 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5461 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5521 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5581 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5641 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5701 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5761 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5821 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5881 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5941 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6001 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6061 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6121 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6181 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6241 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6301 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6361 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6421 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6481 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6541 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6601 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6661 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6721 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6781 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6841 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6901 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6961 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7021 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7081 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7141 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7201 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7261 AACGCGAATT TT
//