LOCUS       MR221642                9264 bp    DNA     circular  SYN 
DEFINITION  Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 1
ACCESSION   NM_053270
VERSION     NM_053270.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5419..5510 
                     /label="Myc-DDK"  
     misc_feature    5540..6125 
                     /label="PolyA signal"  
     misc_feature    5597..5616 
                     /label="Restriction Enzyme"  
     misc_feature    6401..7073 
                     /label="ColE1"  
     misc_feature    7503..8297 
                     /label="Kan/Neo"  
     misc_feature    8332..8634 
                     /label="SV40 ori"  
     misc_feature    8720..9197 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5409..5417 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5409 
                     /label="Rims1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTCGGCC GTGGGGCCCC GAGGTCCTCG CCCACCCACG GTGCCTCCCC 
     1081 CTATGCAAGA ACTGCCCGAC CTGAGCCACC TGACCGAGGA GGAGAGGAAC ATTATCATGG 
     1141 CAGTGATGGA CCGGCAGAAG GAAGAGGAGG AAAAAGAAGA GGCCATGCTC AAGTGTGTTG 
     1201 TCAGGGACAT GGCGAAGCCT GCTGCCTGCA AAACACCAAG AAATGCTGAA AGCCAGCCCC 
     1261 ATCAACCACC ACTGAACATT TTCAGATGTG TCTGTGTTCC CAGAAAGCCA AGCAGCGAAG 
     1321 AGGGAGGCCC AGACAGAAAC TGGAGATTGC ATCAACAGTT TGAAAGCTAC AAGGAACAAG 
     1381 TGAGAAAAAT CGGAGAGGAA GCCAGGCGTT ACCAGGGCGA GCACAAAGAT GATGCCCCGA 
     1441 CGTGTGGAAT CTGTCATAAG ACAAAGTTTG CTGATGGATG TGGCCATCTC TGCTCCTATT 
     1501 GTCGCACCAA GTTCTGTGCA CGCTGTGGAG GCCGAGTGTC TCTGCGATCG AACAATGAGG 
     1561 ACAAAGTGGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAGATA TTAACGAAAT 
     1621 CTGGAGCGTG GTTCTTCGGA AGTGGTCCTC AGCAGCCCAG TCAAGATGGG ACTCTGAGTG 
     1681 ACACGGCTAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCTCGG CTCCAAGAGC 
     1741 GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTTCCC 
     1801 ATGGTGCACC ACTGGACAGG AACAAAGGGG CGGAGCCCTC ACAGCAAGCC TTGGGTCCTG 
     1861 AACAGAAGCA GGCATCAAGG TCAAGAAGTG AGCCACCAAG GGAAAGGAAG AAGGCTCCAG 
     1921 GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGTGA GCGCAAACGT GTCCCCAAGT 
     1981 CTGTGGTGCA ACCCGGGGAA GGGACCGCGG ATGAACGGGA GAGGAAGGAG AGGCGGGAAA 
     2041 CCCGCAGGCT GGAGAAAGGG CGCTCTCAGG ACTACCCGGA CCGGCTCGAG AAACGCGAGG 
     2101 ATGGCAGGGT GGCTGAAGAC GAAAAGCAGA GGAAGGAGGA GGAGGGCGTG TCCACGCCGG 
     2161 AGTACACGAG CTGCGAGGAC GTGGAGCTGG AGAGCGAGAG CGTGAGCGAG AAAGGTGACT 
     2221 TGGACTACTG GTTGGATCCT GCCACGTGGC ATAGCAGGGA AACGTCGCCT ATTAGTTCGC 
     2281 ATCCTGTAAC GTGGCAGCCA TCTAAAGAGG GGGACCGATT AATCGGCCGT GTTATTCTTA 
     2341 ACAAAAGAAC AACCATGCCC AAAGAATCAG GTGCATTATT GGGTCTCAAG GTGGTTGGAG 
     2401 GAAAAATGAC GGACTTAGGG CGTCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGTAGCC 
     2461 TGGCAGATGT TGTCGGACAC CTAAGAGCAG GGGATGAAGT TCTAGAGTGG AATGGTAAAC 
     2521 CCCTGCCGGG AGCAACAAAC GAAGAAGTTT ACAACATTAT TTTAGAATCA AAATCAGAAC 
     2581 CTCAAGTTGA AATTATTGTT TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT 
     2641 CCCACCCTCC TCTGGAGTCC AGTTCAAGTT CCTTTGAATC TCAGAAGATG GAAAGGCCTT 
     2701 CCATTTCTGT TATTTCTCCA ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC 
     2761 CAGGGCAACT CTCAGTGAAG CTGTGGTATG ATAAGGTGGG GCACCAGCTG ATTGTAAATG 
     2821 TTCTACAAGC AACAGATCTA CCCCCTAGAG TAGATGGGCG TCCCAGAAAT CCCTATGTAA 
     2881 AAATGTATTT TCTTCCAGAT AGAAGCGACA AGAGTAAAAG AAGAACCAAA ACGGTAAAGA 
     2941 AACTTCTAGA GCCAAAATGG AATCAAACAT TTGTCTACTC ACACGTACAT CGTCGAGATT 
     3001 TTCGAGAGCG AATGTTAGAA ATTACTGTGT GGGACCAGCC AAGAGTACAG GACGAGGAGA 
     3061 GTGAATTTCT AGGAGAGATC CTCATAGAAT TGGAAACAGC ACTTTTAGAT GATGAGCCCC 
     3121 ATTGGTATAA ACTGCAGACA CATGATGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT 
     3181 TCATGCCCAG GCGGCATATC CACGGAGAAA GCTCCAGCAA AAAGCTACAA AGATCTCAGC 
     3241 GAATCAGTGA TAGTGACATC TCAGATTATG AGGTTGATGA TGGTATTGGC GTAGTGCCTC 
     3301 CAGTGGGTTA TAGAGCTAGT GCTAGAGAGA GTAAAGCCAC CACGTTAACA GTGCCAGAGC 
     3361 AGCAAAGAAC CACACATCAC CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG 
     3421 GAAGGCCTCG TTCACGTTTA CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATCCATC 
     3481 CAACACGAAG GTCACGTTCT CCAACCCGAC ACCATGATGC CTCCCGAAGC CTGGCCGATC 
     3541 ACAGATCACG ACATGCGGAA AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC 
     3601 TGCCCAGAGC AAAACGAGGA CGAAGTGCAG AATGCCTACA CATGACCAGT GAACTGCAGC 
     3661 CCTCTCTTGA CAGGGCTAGG AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC 
     3721 ATTCACCAGA ACGAGAAAGG GGTAGATGGT CCCCCTCCCT AGCTAGGAGG CGACCTGCTA 
     3781 GCCCCAGGAT TCAAATCCAG CATGCGTCTC CGGAGAATGA CAGGCACTCC AGAAAGTCTG 
     3841 AAAGATCTAG CATCCAAAAA CAGTCTAGGA AAGGCACAGC CTCTGATGCA GACAGGGTTC 
     3901 TCCCACCATG CCTTTCTAGA AGGGGATACG CAATCCCAAG AGCAACCGAT CAACCGGTCA 
     3961 TTAGGGGAAA GCATACCACT CGCTCCCGGT CGAGCGAGCA CTCTAGTATC AGAACCCTGT 
     4021 GTTCTATGCA CCACCTTGCC CCCGGAGGGT CAGCGCCGCC TTCTCCGCTT CTGACAAGAA 
     4081 CGCACCGACA AGGAAGCCCC ACCCAGTCAC CTCCAGCTGA CACATCCTTT GGCAGTCGCC 
     4141 GCGGAAGACA GCTCCCACAG GTGCCGGTCC GAAGCGGCAG TATAGAACAA GCGAGCTTAG 
     4201 TAGTGGAGGA ACGAACGAGA CAGATGAAAA TGAAGGTACA CCGATTTAAG CAGACAACAG 
     4261 GGTCTGGGTC TAGTCAAGAA CTTGACCACG AGCAATACTC CAAGTACAAC ATACATAAAG 
     4321 ATCAGTACAG AAGCTGTGAT AACGCGTCCG CCAAGTCATC AGATAGTGAT GTCAGTGATG 
     4381 TGTCCGCCAT TTCCAGAGCC AGCAGCACCT CACGCCTCAG CAGCACAAGC TTTATGTCAG 
     4441 AGCAGTCTGA GCGCCCCAGG GGCAGGATCA GTTCATTTAC CCCCAAAATG CAAGGCAGAC 
     4501 GGATGGGGAC TTCAGGAAGA GCCATCATCA AGAGCACCAG TGTAAGTGGA GAGATATATA 
     4561 CACTGGAGCA CAATGACGGC AGCCAGTCCG ACACGGCCGT GGGTACCGTT GGAGCTGGTG 
     4621 GAAAGAAGCG GAGGTCCAGC CTGAGTGCCA AAGTGGTAGC CATTGTGTCT CGAAGAAGCA 
     4681 GAAGCACATC ACAGCTCAGC CAGACAGAGT CCGGCCACAA GAAGTTAAAA AGCACCATTC 
     4741 AGAGGAGTAC GGAAACGGGA ATGGCGGCTG AAATGCGGAA GATGGTGAGA CAGCCCAGCC 
     4801 GGGAGTCCAC GGATGGCAGC ATCAACAGTT ATAGCTCAGA AGGAAACTTA ATATTTCCTG 
     4861 GAGTTCGCGT GGGACCTGAC AGTCAGTTCA GTGATTTCCT TGATGGACTG GGGCCAGCCC 
     4921 AGCTTGTCGG CCGCCAAACC CTAGCCACCC CAGCCATGGG CGATATCCAA ATCGGGATGG 
     4981 AGGATAAGAA GGGTCAGTTG GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC 
     5041 CTGGCTCCAA ATCTACACCT GCTCCCTATG TGAAAGTTTA TCTTTTGGAA AATGGAGCCT 
     5101 GTATTGCCAA AAAGAAGACA AGAATTGCAC GGAAAACTCT CGATCCCTTG TATCAGCAGT 
     5161 CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT 
     5221 ATGGAAGAAT GGACCACAAA TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG 
     5281 ATCTGTCCAG CATGGTGATT GGATGGTATA AATTGTTCCC TCCATCCTCC CTGGTGGATC 
     5341 CCACACTCAC TCCCCTGACC CGCCGGGCTT CCCAATCATC ACTGGAAAGT TCGTCTGGGC 
     5401 CTCCCTGCAT CCGGTCAACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5461 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5521 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5581 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5641 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5701 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5761 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5821 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5881 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5941 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     6001 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6061 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6121 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6181 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6241 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6301 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6361 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6421 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6481 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6541 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6601 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6661 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6721 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6781 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6841 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6901 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6961 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     7021 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7081 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7141 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7201 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7261 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7321 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7381 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7441 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7501 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7561 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7621 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7681 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7741 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7801 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7861 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7921 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7981 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     8041 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8101 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8161 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8221 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8281 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8341 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8401 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8461 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8521 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8581 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8641 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8701 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8761 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8821 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8881 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8941 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     9001 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9061 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9121 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9181 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9241 TAACAAAAAT TTAACGCGAA TTTT
//