LOCUS       MR221629                8772 bp    DNA     circular  SYN 
DEFINITION  Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 5
ACCESSION   NM_001012625
VERSION     NM_001012625.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4927..5018 
                     /label="Myc-DDK"  
     misc_feature    5048..5633 
                     /label="PolyA signal"  
     misc_feature    5105..5124 
                     /label="Restriction Enzyme"  
     misc_feature    5909..6581 
                     /label="ColE1"  
     misc_feature    7011..7805 
                     /label="Kan/Neo"  
     misc_feature    7840..8142 
                     /label="SV40 ori"  
     misc_feature    8228..8705 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4917..4925 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4917 
                     /label="Rims1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTCGGCC GTGGGGCCCC GAGGTCCTCG CCCACCCACG GTGCCTCCCC 
     1081 CTATGCAAGA ACTGCCCGAC CTGAGCCACC TGACCGAGGA GGAGAGGAAC ATTATCATGG 
     1141 CAGTGATGGA CCGGCAGAAG GAAGAGGAGG AAAAAGAAGA GGCCATGCTC AAGTGTGTTG 
     1201 TCAGGGACAT GGCGAAGCCT GCTGCCTGCA AAACACCAAG AAATGCTGAA AGCCAGCCCC 
     1261 ATCAACCACC ACTGAACATT TTCAGATGTG TCTGTGTTCC CAGAAAGCCA AGCAGCGAAG 
     1321 AGGGAGGCCC AGACAGAAAC TGGAGATTGC ATCAACAGTT TGAAAGCTAC AAGGAACAAG 
     1381 TGAGAAAAAT CGGAGAGGAA GCCAGGCGTT ACCAGGGCGA GCACAAAGAT GATGCCCCGA 
     1441 CGTGTGGAAT CTGTCATAAG ACAAAGTTTG CTGATGGATG TGGCCATCTC TGCTCCTATT 
     1501 GTCGCACCAA GTTCTGTGCA CGCTGTGGAG GCCGAGTGTC TCTGCGATCG AACAATGAGG 
     1561 ACAAAGTGGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAGATA TTAACGAAAT 
     1621 CTGGAGCGTG GTTCTTCGGA AGTGGTCCTC AGCAGCCCAG TCAAGATGGG ACTCTGAGTG 
     1681 ACACGGCTAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCTCGG CTCCAAGAGC 
     1741 GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTTCCC 
     1801 ATGGTGCACC ACTGGACAGG AACAAAGGGG CGGAGCCCTC ACAGCAAGCC TTGGGTCCTG 
     1861 AACAGAAGCA GGCATCAAGG TCAAGAAGTG AGCCACCAAG GGAAAGGAAG AAGGCTCCAG 
     1921 GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGTGA GCGCAAACGT GTCCCCAAGT 
     1981 CTGTGGTGCA ACCCGGGGAA GGGACCGCGG ATGAACGGGA GAGGAAGGAG AGGCGGGAAA 
     2041 CCCGCAGGCT GGAGAAAGGG CGCTCTCAGG ACTACCCGGA CCGGCTCGAG AAACGCGAGG 
     2101 ATGGCAGGGT GGCTGAAGAC GAAAAGCAGA GGAAGGAGGA GGAGGGCGTG TCCACGCCGG 
     2161 AGTACACGAG CTGCGAGGAC GTGGAGCTGG AGAGCGAGAG CGTGAGCGAG AAAGGTGACT 
     2221 TGGACTACTG GTTGGATCCT GCCACGTGGC ATAGCAGGGA AACGTCGCCT ATTAGTTCGC 
     2281 ATCCTGTAAC GTGGCAGCCA TCTAAAGAGG GGGACCGATT AATCGGCCGT GTTATTCTTA 
     2341 ACAAAAGAAC AACCATGCCC AAAGAATCAG GTGCATTATT GGGTCTCAAG GTGGTTGGAG 
     2401 GAAAAATGAC GGACTTAGGG CGTCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGTAGCC 
     2461 TGGCAGATGT TGTCGGACAC CTAAGAGCAG GGGATGAAGT TCTAGAGTGG AATGGTAAAC 
     2521 CCCTGCCGGG AGCAACAAAC GAAGAAGTTT ACAACATTAT TTTAGAATCA AAATCAGAAC 
     2581 CTCAAGTTGA AATTATTGTT TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT 
     2641 CCCACCCTCC TCTGGAGTCC AGTTCAAGTT CCTTTGAATC TCAGAAGATG GAAAGGCCTT 
     2701 CCATTTCTGT TATTTCTCCA ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC 
     2761 CAGGGCAACT CTCAGTGAAG CTGTGGTATG ATAAGGTGGG GCACCAGCTG ATTGTAAATG 
     2821 TTCTACAAGC AACAGATCTA CCCCCTAGAG TAGATGGGCG TCCCAGAAAT CCCTATGTAA 
     2881 AAATGTATTT TCTTCCAGAT AGAAGCGACA AGAGTAAAAG AAGAACCAAA ACGGTAAAGA 
     2941 AACTTCTAGA GCCAAAATGG AATCAAACAT TTGTCTACTC ACACGTACAT CGTCGAGATT 
     3001 TTCGAGAGCG AATGTTAGAA ATTACTGTGT GGGACCAGCC AAGAGTACAG GACGAGGAGA 
     3061 GTGAATTTCT AGGAGAGATC CTCATAGAAT TGGAAACAGC ACTTTTAGAT GATGAGCCCC 
     3121 ATTGGTATAA ACTGCAGACA CATGATGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT 
     3181 TCATGCCCAG GCGGCATATC CACGGAGAAA GCTCCAGCAA AAAGCTACAA AGATCTCAGC 
     3241 GAATCAGTGA TAGTGACATC TCAGATTATG AGGTTGATGA TGGTATTGGC GTAGTGCCTC 
     3301 CAGTGGGTTA TAGAGCTAGT GCTAGAGAGA GTAAAGCCAC CACGTTAACA GTGCCAGAGC 
     3361 AGCAAAGAAC CACACATCAC CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG 
     3421 GAAGGCCTCG TTCACGTTTA CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATCCATC 
     3481 CAACACGAAG GTCACGTTCT CCAACCCGAC ACCATGATGC CTCCCGAAGC CTGGCCGATC 
     3541 ACAGATCACG ACATGCGGAA AGTCAATATT CGTCAGAGCC AGACAGAACG CACCGACAAG 
     3601 GAAGCCCCAC CCAGTCACCT CCAGCTGACA CATCCTTTGG CAGTCGCCGC GGAAGACAGC 
     3661 TCCCACAGGT GCCGGTCCGA AGCGGCAGTA TAGAACAAGC GAGCTTAGTA GTGGAGGAAC 
     3721 GAACGAGACA GATGAAAATG AAGGTACACC GATTTAAGCA GACAACAGGG TCTGGGTCTA 
     3781 GTCAAGAACT TGACCACGAG CAATACTCCA AGTACAACAT ACATAAAGAT CAGTACAGAA 
     3841 GCTGTGATAA CGCGTCCGCC AAGTCATCAG ATAGTGATGT CAGTGATGTG TCCGCCATTT 
     3901 CCAGAGCCAG CAGCACCTCA CGCCTCAGCA GCACAAGCTT TATGTCAGAG CAGTCTGAGC 
     3961 GCCCCAGGGG CAGGATCAGT TCATTTACCC CCAAAATGCA AGGCAGACGG ATGGGGACTT 
     4021 CAGGAAGAGC CATCATCAAG AGCACCAGTG TAAGTGGAGA GATATATACA CTGGAGCACA 
     4081 ATGACGGCAG CCAGTCCGAC ACGGCCGTGG GTACCGTTGG AGCTGGTGGA AAGAAGCGGA 
     4141 GGTCCAGCCT GAGTGCCAAA GTGGTAGCCA TTGTGTCTCG AAGAAGCAGA AGCACATCAC 
     4201 AGCTCAGCCA GACAGAGTCC GGCCACAAGA AGTTAAAAAG CACCATTCAG AGGAGTACGG 
     4261 AAACGGGAAT GGCGGCTGAA ATGCGGAAGA TGGTGAGACA GCCCAGCCGG GAGTCCACGG 
     4321 ATGGCAGCAT CAACAGTTAT AGCTCAGAAG GAAACTTAAT ATTTCCTGGA GTTCGCGTGG 
     4381 GACCTGACAG TCAGTTCAGT GATTTCCTTG ATGGACTGGG GCCAGCCCAG CTTGTCGGCC 
     4441 GCCAAACCCT AGCCACCCCA GCCATGGGCG ATATCCAAAT CGGGATGGAG GATAAGAAGG 
     4501 GTCAGTTGGA GGTTGAGGTT ATCAGAGCCC GGAGCCTTAC ACAAAAACCT GGCTCCAAAT 
     4561 CTACACCTGC TCCCTATGTG AAAGTTTATC TTTTGGAAAA TGGAGCCTGT ATTGCCAAAA 
     4621 AGAAGACAAG AATTGCACGG AAAACTCTCG ATCCCTTGTA TCAGCAGTCC CTGGTTTTTG 
     4681 ATGAAAGTCC ACAGGGTAAA GTTCTTCAGG TGATTGTCTG GGGTGACTAT GGAAGAATGG 
     4741 ACCACAAATG CTTTATGGGT GTGGCTCAAA TCTTGTTGGA AGAACTTGAT CTGTCCAGCA 
     4801 TGGTGATTGG ATGGTATAAA TTGTTCCCTC CATCCTCCCT GGTGGATCCC ACACTCACTC 
     4861 CCCTGACCCG CCGGGCTTCC CAATCATCAC TGGAAAGTTC GTCTGGGCCT CCCTGCATCC 
     4921 GGTCAACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4981 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5041 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5101 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5161 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5221 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5281 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5341 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5401 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5461 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5521 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5581 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5641 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5701 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5761 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5821 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5881 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5941 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6001 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6061 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6121 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6181 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6241 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6301 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6361 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6421 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6481 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6541 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6601 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6661 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6721 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6781 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6841 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6901 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6961 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7021 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7081 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7141 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7201 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7261 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7321 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7381 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7441 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7501 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7561 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7621 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7681 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7741 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7801 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7861 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7921 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7981 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8221 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8281 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8341 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8401 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8461 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8521 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8581 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8641 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8701 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8761 AACGCGAATT TT
//