LOCUS       MR221627                8925 bp    DNA     circular  SYN 
DEFINITION  Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 4
ACCESSION   NM_001012624
VERSION     NM_001012624.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5080..5171 
                     /label="Myc-DDK"  
     misc_feature    5201..5786 
                     /label="PolyA signal"  
     misc_feature    5258..5277 
                     /label="Restriction Enzyme"  
     misc_feature    6062..6734 
                     /label="ColE1"  
     misc_feature    7164..7958 
                     /label="Kan/Neo"  
     misc_feature    7993..8295 
                     /label="SV40 ori"  
     misc_feature    8381..8858 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5070..5078 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5070 
                     /label="Rims1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTCGGCC GTGGGGCCCC GAGGTCCTCG CCCACCCACG GTGCCTCCCC 
     1081 CTATGCAAGA ACTGCCCGAC CTGAGCCACC TGACCGAGGA GGAGAGGAAC ATTATCATGG 
     1141 CAGTGATGGA CCGGCAGAAG GAAGAGGAGG AAAAAGAAGA GGCCATGCTC AAGTGTGTTG 
     1201 TCAGGGACAT GGCGAAGCCT GCTGCCTGCA AAACACCAAG AAATGCTGAA AGCCAGCCCC 
     1261 ATCAACCACC ACTGAACATT TTCAGATGTG TCTGTGTTCC CAGAAAGCCA AGCAGCGAAG 
     1321 AGGGAGGCCC AGACAGAAAC TGGAGATTGC ATCAACAGTT TGAAAGCTAC AAGGAACAAG 
     1381 TGAGAAAAAT CGGAGAGGAA GCCAGGCGTT ACCAGGGCGA GCACAAAGAT GATGCCCCGA 
     1441 CGTGTGGAAT CTGTCATAAG ACAAAGTTTG CTGATGGATG TGGCCATCTC TGCTCCTATT 
     1501 GTCGCACCAA GTTCTGTGCA CGCTGTGGAG GCCGAGTGTC TCTGCGATCG AACAATGAGG 
     1561 ACAAAGTGGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAGATA TTAACGAAAT 
     1621 CTGGAGCGTG GTTCTTCGGA AGTGGTCCTC AGCAGCCCAG TCAAGATGGG ACTCTGAGTG 
     1681 ACACGGCTAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCTCGG CTCCAAGAGC 
     1741 GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTTCCC 
     1801 ATGGTGCACC ACTGGACAGG AACAAAGGGG CGGAGCCCTC ACAGCAAGCC TTGGGTCCTG 
     1861 AACAGAAGCA GGCATCAAGG TCAAGAAGTG AGCCACCAAG GGAAAGGAAG AAGGCTCCAG 
     1921 GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGTGA GCGCAAACGT GTCCCCAAGT 
     1981 CTGTGGTGCA ACCCGGGGAA GGGACCGCGG ATGAACGGGA GAGGAAGGAG AGGCGGGAAA 
     2041 CCCGCAGGCT GGAGAAAGGG CGCTCTCAGG ACTACCCGGA CCGGCTCGAG AAACGCGAGG 
     2101 ATGGCAGGGT GGCTGAAGAC GAAAAGCAGA GGAAGGAGGA GGAGGGCGTG TCCACGCCGG 
     2161 AGTACACGAG CTGCGAGGAC GTGGAGCTGG AGAGCGAGAG CGTGAGCGAG AAAGGTGACT 
     2221 TGGACTACTG GTTGGATCCT GCCACGTGGC ATAGCAGGGA AACGTCGCCT ATTAGTTCGC 
     2281 ATCCTGTAAC GTGGCAGCCA TCTAAAGAGG GGGACCGATT AATCGGCCGT GTTATTCTTA 
     2341 ACAAAAGAAC AACCATGCCC AAAGAATCAG GTGCATTATT GGGTCTCAAG GTGGTTGGAG 
     2401 GAAAAATGAC GGACTTAGGG CGTCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGTAGCC 
     2461 TGGCAGATGT TGTCGGACAC CTAAGAGCAG GGGATGAAGT TCTAGAGTGG AATGGTAAAC 
     2521 CCCTGCCGGG AGCAACAAAC GAAGAAGTTT ACAACATTAT TTTAGAATCA AAATCAGAAC 
     2581 CTCAAGTTGA AATTATTGTT TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT 
     2641 CCCACCCTCC TCTGGAGTCC AGTTCAAGTT CCTTTGAATC TCAGAAGATG GAAAGGCCTT 
     2701 CCATTTCTGT TATTTCTCCA ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC 
     2761 CAGGGCAACT CTCAGTGAAG CTGTGGTATG ATAAGGTGGG GCACCAGCTG ATTGTAAATG 
     2821 TTCTACAAGC AACAGATCTA CCCCCTAGAG TAGATGGGCG TCCCAGAAAT CCCTATGTAA 
     2881 AAATGTATTT TCTTCCAGAT AGAAGCGACA AGAGTAAAAG AAGAACCAAA ACGGTAAAGA 
     2941 AACTTCTAGA GCCAAAATGG AATCAAACAT TTGTCTACTC ACACGTACAT CGTCGAGATT 
     3001 TTCGAGAGCG AATGTTAGAA ATTACTGTGT GGGACCAGCC AAGAGTACAG GACGAGGAGA 
     3061 GTGAATTTCT AGGAGAGATC CTCATAGAAT TGGAAACAGC ACTTTTAGAT GATGAGCCCC 
     3121 ATTGGTATAA ACTGCAGACA CATGATGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT 
     3181 TCATGCCCAG GCGGCATATC CACGGAGAAA GCTCCAGCAA AAAGCTACAA AGATCTCAGC 
     3241 GAATCAGTGA TAGTGACATC TCAGATTATG AGGTTGATGA TGGTATTGGC GTAGTGCCTC 
     3301 CAGTGGGTTA TAGAGCTAGT GCTAGAGAGA GTAAAGCCAC CACGTTAACA GTGCCAGAGC 
     3361 AGCAAAGAAC CACACATCAC CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG 
     3421 GAAGGCCTCG TTCACGTTTA CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATCCATC 
     3481 CAACACGAAG GTCACGTTCT CCAACCCGAC ACCATGATGC CTCCCGAAGC CTGGCCGATC 
     3541 ACAGATCACG ACATGCGGAA AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC 
     3601 TGCCCAGAGC AAAACGAGGA CGAAGTGCAG AATGCCTACA CATGACCAGT GAACTGCAGC 
     3661 CCTCTCTTGA CAGGGCTAGG AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC 
     3721 ATTCACCAGA ACGAGAAAGG ACGCACCGAC AAGGAAGCCC CACCCAGTCA CCTCCAGCTG 
     3781 ACACATCCTT TGGCAGTCGC CGCGGAAGAC AGCTCCCACA GGTGCCGGTC CGAAGCGGCA 
     3841 GTATAGAACA AGCGAGCTTA GTAGTGGAGG AACGAACGAG ACAGATGAAA ATGAAGGTAC 
     3901 ACCGATTTAA GCAGACAACA GGGTCTGGGT CTAGTCAAGA ACTTGACCAC GAGCAATACT 
     3961 CCAAGTACAA CATACATAAA GATCAGTACA GAAGCTGTGA TAACGCGTCC GCCAAGTCAT 
     4021 CAGATAGTGA TGTCAGTGAT GTGTCCGCCA TTTCCAGAGC CAGCAGCACC TCACGCCTCA 
     4081 GCAGCACAAG CTTTATGTCA GAGCAGTCTG AGCGCCCCAG GGGCAGGATC AGTTCATTTA 
     4141 CCCCCAAAAT GCAAGGCAGA CGGATGGGGA CTTCAGGAAG AGCCATCATC AAGAGCACCA 
     4201 GTGTAAGTGG AGAGATATAT ACACTGGAGC ACAATGACGG CAGCCAGTCC GACACGGCCG 
     4261 TGGGTACCGT TGGAGCTGGT GGAAAGAAGC GGAGGTCCAG CCTGAGTGCC AAAGTGGTAG 
     4321 CCATTGTGTC TCGAAGAAGC AGAAGCACAT CACAGCTCAG CCAGACAGAG TCCGGCCACA 
     4381 AGAAGTTAAA AAGCACCATT CAGAGGAGTA CGGAAACGGG AATGGCGGCT GAAATGCGGA 
     4441 AGATGGTGAG ACAGCCCAGC CGGGAGTCCA CGGATGGCAG CATCAACAGT TATAGCTCAG 
     4501 AAGGAAACTT AATATTTCCT GGAGTTCGCG TGGGACCTGA CAGTCAGTTC AGTGATTTCC 
     4561 TTGATGGACT GGGGCCAGCC CAGCTTGTCG GCCGCCAAAC CCTAGCCACC CCAGCCATGG 
     4621 GCGATATCCA AATCGGGATG GAGGATAAGA AGGGTCAGTT GGAGGTTGAG GTTATCAGAG 
     4681 CCCGGAGCCT TACACAAAAA CCTGGCTCCA AATCTACACC TGCTCCCTAT GTGAAAGTTT 
     4741 ATCTTTTGGA AAATGGAGCC TGTATTGCCA AAAAGAAGAC AAGAATTGCA CGGAAAACTC 
     4801 TCGATCCCTT GTATCAGCAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTTCTTC 
     4861 AGGTGATTGT CTGGGGTGAC TATGGAAGAA TGGACCACAA ATGCTTTATG GGTGTGGCTC 
     4921 AAATCTTGTT GGAAGAACTT GATCTGTCCA GCATGGTGAT TGGATGGTAT AAATTGTTCC 
     4981 CTCCATCCTC CCTGGTGGAT CCCACACTCA CTCCCCTGAC CCGCCGGGCT TCCCAATCAT 
     5041 CACTGGAAAG TTCGTCTGGG CCTCCCTGCA TCCGGTCAAC GCGGCCGCTC GAGCAGAAAC 
     5101 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5161 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5221 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5281 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5341 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5401 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5461 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5521 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5581 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5641 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5701 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5761 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5821 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5881 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5941 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6001 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6061 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6121 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6181 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6241 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6301 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6361 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6421 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6481 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6541 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6601 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6661 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6721 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6781 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6841 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6901 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6961 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7021 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7081 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7141 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7201 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7261 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7321 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7381 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7441 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7501 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7561 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7621 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7681 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7741 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7801 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7861 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7921 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7981 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8041 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8101 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8161 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8221 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8281 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8341 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8401 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8461 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8521 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8581 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8641 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8701 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8761 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8821 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8881 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//