LOCUS       MR221626                9180 bp    DNA     circular  SYN 
DEFINITION  Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 2
ACCESSION   NM_183018
VERSION     NM_183018.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5335..5426 
                     /label="Myc-DDK"  
     misc_feature    5456..6041 
                     /label="PolyA signal"  
     misc_feature    5513..5532 
                     /label="Restriction Enzyme"  
     misc_feature    6317..6989 
                     /label="ColE1"  
     misc_feature    7419..8213 
                     /label="Kan/Neo"  
     misc_feature    8248..8550 
                     /label="SV40 ori"  
     misc_feature    8636..9113 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5325..5333 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5325 
                     /label="Rims1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTCGGCC GTGGGGCCCC GAGGTCCTCG CCCACCCACG GTGCCTCCCC 
     1081 CTATGCAAGA ACTGCCCGAC CTGAGCCACC TGACCGAGGA GGAGAGGAAC ATTATCATGG 
     1141 CAGTGATGGA CCGGCAGAAG GAAGAGGAGG AAAAAGAAGA GGCCATGCTC AAGTGTGTTG 
     1201 TCAGGGACAT GGCGAAGCCT GCTGCCTGCA AAACACCAAG AAATGCTGAA AGCCAGCCCC 
     1261 ATCAACCACC ACTGAACATT TTCAGATGTG TCTGTGTTCC CAGAAAGCCA AGCAGCGAAG 
     1321 AGGGAGGCCC AGACAGAAAC TGGAGATTGC ATCAACAGTT TGAAAGCTAC AAGGAACAAG 
     1381 TGAGAAAAAT CGGAGAGGAA GCCAGGCGTT ACCAGGGCGA GCACAAAGAT GATGCCCCGA 
     1441 CGTGTGGAAT CTGTCATAAG ACAAAGTTTG CTGATGGATG TGGCCATCTC TGCTCCTATT 
     1501 GTCGCACCAA GTTCTGTGCA CGCTGTGGAG GCCGAGTGTC TCTGCGATCG AACAATGAGG 
     1561 ACAAAGTGGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAGATA TTAACGAAAT 
     1621 CTGGAGCGTG GTTCTTCGGA AGTGGTCCTC AGCAGCCCAG TCAAGATGGG ACTCTGAGTG 
     1681 ACACGGCTAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCTCGG CTCCAAGAGC 
     1741 GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTTCCC 
     1801 ATGGTGCACC ACTGGACAGG AACAAAGGGG CGGAGCCCTC ACAGCAAGCC TTGGGTCCTG 
     1861 AACAGAAGCA GGCATCAAGG TCAAGAAGTG AGCCACCAAG GGAAAGGAAG AAGGCTCCAG 
     1921 GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGTGA GCGCAAACGT GTCCCCAAGT 
     1981 CTGTGGTGCA ACCCGGGGAA GGGACCGCGG ATGAACGGGA GAGGAAGGAG AGGCGGGAAA 
     2041 CCCGCAGGCT GGAGAAAGGG CGCTCTCAGG ACTACCCGGA CCGGCTCGAG AAACGCGAGG 
     2101 ATGGCAGGGT GGCTGAAGAC GAAAAGCAGA GGAAGGAGGA GGAGGGCGTG TCCACGCCGG 
     2161 AGTACACGAG CTGCGAGGAC GTGGAGCTGG AGAGCGAGAG CGTGAGCGAG AAAGGTGACT 
     2221 TGGACTACTG GTTGGATCCT GCCACGTGGC ATAGCAGGGA AACGTCGCCT ATTAGTTCGC 
     2281 ATCCTGTAAC GTGGCAGCCA TCTAAAGAGG GGGACCGATT AATCGGCCGT GTTATTCTTA 
     2341 ACAAAAGAAC AACCATGCCC AAAGAATCAG GTGCATTATT GGGTCTCAAG GTGGTTGGAG 
     2401 GAAAAATGAC GGACTTAGGG CGTCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGTAGCC 
     2461 TGGCAGATGT TGTCGGACAC CTAAGAGCAG GGGATGAAGT TCTAGAGTGG AATGGTAAAC 
     2521 CCCTGCCGGG AGCAACAAAC GAAGAAGTTT ACAACATTAT TTTAGAATCA AAATCAGAAC 
     2581 CTCAAGTTGA AATTATTGTT TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT 
     2641 CCCACCCTCC TCTGGAGTCC AGTTCAAGTT CCTTTGAATC TCAGAAGATG GAAAGGCCTT 
     2701 CCATTTCTGT TATTTCTCCA ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC 
     2761 CAGGGCAACT CTCAGTGAAG CTGTGGTATG ATAAGGTGGG GCACCAGCTG ATTGTAAATG 
     2821 TTCTACAAGC AACAGATCTA CCCCCTAGAG TAGATGGGCG TCCCAGAAAT CCCTATGTAA 
     2881 AAATGTATTT TCTTCCAGAT AGAAGCGACA AGAGTAAAAG AAGAACCAAA ACGGTAAAGA 
     2941 AACTTCTAGA GCCAAAATGG AATCAAACAT TTGTCTACTC ACACGTACAT CGTCGAGATT 
     3001 TTCGAGAGCG AATGTTAGAA ATTACTGTGT GGGACCAGCC AAGAGTACAG GACGAGGAGA 
     3061 GTGAATTTCT AGGAGAGATC CTCATAGAAT TGGAAACAGC ACTTTTAGAT GATGAGCCCC 
     3121 ATTGGTATAA ACTGCAGACA CATGATGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT 
     3181 TCATGCCCAG GCGGCATATC CACGGAGAAA GCTCCAGCAA AAAGCTACAA AGATCTCAGC 
     3241 GAATCAGTGA TAGTGACATC TCAGATTATG AGGTTGATGA TGGTATTGGC GTAGTGCCTC 
     3301 CAGTGGGTTA TAGAGCTAGT GCTAGAGAGA GTAAAGCCAC CACGTTAACA GTGCCAGAGC 
     3361 AGCAAAGAAC CACACATCAC CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG 
     3421 GAAGGCCTCG TTCACGTTTA CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATCCATC 
     3481 CAACACGAAG GTCACGTTCT CCAACCCGAC ACCATGATGC CTCCCGAAGC CTGGCCGATC 
     3541 ACAGATCACG ACATGCGGAA AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC 
     3601 TGCCCAGAGC AAAACGAGGA CGAAGTGCAG AATGCCTACA CATGACCAGT GAACTGCAGC 
     3661 CCTCTCTTGA CAGGGCTAGG AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC 
     3721 ATTCACCAGA ACGAGAAAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAAACAGT 
     3781 CTAGGAAAGG CACAGCCTCT GATGCAGACA GGGTTCTCCC ACCATGCCTT TCTAGAAGGG 
     3841 GATACGCAAT CCCAAGAGCA ACCGATCAAC CGGTCATTAG GGGAAAGCAT ACCACTCGCT 
     3901 CCCGGTCGAG CGAGCACTCT AGTATCAGAA CCCTGTGTTC TATGCACCAC CTTGCCCCCG 
     3961 GAGGGTCAGC GCCGCCTTCT CCGCTTCTGA CAAGAACGCA CCGACAAGGA AGCCCCACCC 
     4021 AGTCACCTCC AGCTGACACA TCCTTTGGCA GTCGCCGCGG AAGACAGCTC CCACAGGTGC 
     4081 CGGTCCGAAG CGGCAGTATA GAACAAGCGA GCTTAGTAGT GGAGGAACGA ACGAGACAGA 
     4141 TGAAAATGAA GGTACACCGA TTTAAGCAGA CAACAGGGTC TGGGTCTAGT CAAGAACTTG 
     4201 ACCACGAGCA ATACTCCAAG TACAACATAC ATAAAGATCA GTACAGAAGC TGTGATAACG 
     4261 CGTCCGCCAA GTCATCAGAT AGTGATGTCA GTGATGTGTC CGCCATTTCC AGAGCCAGCA 
     4321 GCACCTCACG CCTCAGCAGC ACAAGCTTTA TGTCAGAGCA GTCTGAGCGC CCCAGGGGCA 
     4381 GGATCAGTTC ATTTACCCCC AAAATGCAAG GCAGACGGAT GGGGACTTCA GGAAGAGCCA 
     4441 TCATCAAGAG CACCAGTGTA AGTGGAGAGA TATATACACT GGAGCACAAT GACGGCAGCC 
     4501 AGTCCGACAC GGCCGTGGGT ACCGTTGGAG CTGGTGGAAA GAAGCGGAGG TCCAGCCTGA 
     4561 GTGCCAAAGT GGTAGCCATT GTGTCTCGAA GAAGCAGAAG CACATCACAG CTCAGCCAGA 
     4621 CAGAGTCCGG CCACAAGAAG TTAAAAAGCA CCATTCAGAG GAGTACGGAA ACGGGAATGG 
     4681 CGGCTGAAAT GCGGAAGATG GTGAGACAGC CCAGCCGGGA GTCCACGGAT GGCAGCATCA 
     4741 ACAGTTATAG CTCAGAAGGA AACTTAATAT TTCCTGGAGT TCGCGTGGGA CCTGACAGTC 
     4801 AGTTCAGTGA TTTCCTTGAT GGACTGGGGC CAGCCCAGCT TGTCGGCCGC CAAACCCTAG 
     4861 CCACCCCAGC CATGGGCGAT ATCCAAATCG GGATGGAGGA TAAGAAGGGT CAGTTGGAGG 
     4921 TTGAGGTTAT CAGAGCCCGG AGCCTTACAC AAAAACCTGG CTCCAAATCT ACACCTGCTC 
     4981 CCTATGTGAA AGTTTATCTT TTGGAAAATG GAGCCTGTAT TGCCAAAAAG AAGACAAGAA 
     5041 TTGCACGGAA AACTCTCGAT CCCTTGTATC AGCAGTCCCT GGTTTTTGAT GAAAGTCCAC 
     5101 AGGGTAAAGT TCTTCAGGTG ATTGTCTGGG GTGACTATGG AAGAATGGAC CACAAATGCT 
     5161 TTATGGGTGT GGCTCAAATC TTGTTGGAAG AACTTGATCT GTCCAGCATG GTGATTGGAT 
     5221 GGTATAAATT GTTCCCTCCA TCCTCCCTGG TGGATCCCAC ACTCACTCCC CTGACCCGCC 
     5281 GGGCTTCCCA ATCATCACTG GAAAGTTCGT CTGGGCCTCC CTGCATCCGG TCAACGCGGC 
     5341 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5401 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5461 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5521 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5581 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5641 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5701 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5761 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5821 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5881 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5941 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6001 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6061 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6121 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6181 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6241 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6301 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6361 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6421 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6481 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6541 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6601 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6661 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6721 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6781 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6841 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6901 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6961 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7021 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7081 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7141 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7201 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7261 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7321 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7381 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7441 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7501 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7561 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7621 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7681 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7741 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7801 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7861 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7921 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7981 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8041 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8101 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8161 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8221 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8281 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8341 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8401 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8461 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8521 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8581 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8641 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8701 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8761 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8821 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8881 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8941 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9001 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9061 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9121 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//