LOCUS       MR221610                7974 bp    DNA     circular  SYN 
DEFINITION  Atp4a (Myc-DDK-tagged) - Mouse ATPase, H+/K+ exchanging, gastric, alpha polypeptide (Atp4a)
ACCESSION   NM_018731
VERSION     NM_018731.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4129..4220 
                     /label="Myc-DDK"  
     misc_feature    4250..4835 
                     /label="PolyA signal"  
     misc_feature    4307..4326 
                     /label="Restriction Enzyme"  
     misc_feature    5111..5783 
                     /label="ColE1"  
     misc_feature    6213..7007 
                     /label="Kan/Neo"  
     misc_feature    7042..7344 
                     /label="SV40 ori"  
     misc_feature    7430..7907 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4104..4111 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4104 
                     /label="Atp4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GGGAAGGCAG AGAATTATGA ACTGTACTCA GTGGAACTGG 
     1081 GGTCTGGCCC TGGTGGGGAC ATGACTGCCA AGATGAGCAA GAAGAAGGCA GGAGGTGGGG 
     1141 GAGGCAAGAA GAAGGAGAAG CTGGAGAACA TGAAGAAGGA GATGGAGATT AACGACCACC 
     1201 AGCTATCAGT GTCTGAGCTG GAGCAGAAGT ATCAGACCAG CGCCACCAAG GGCCTGAAGG 
     1261 CGAGCCTGGC AGCTGAGCTG CTGCTGAGGG ATGGACCCAA TGCGCTCCGG CCACCTCGGG 
     1321 GCACTCCCGA GTATGTGAAG TTTGCCCGTC AGCTGGCAGG TGGTCTGCAG TGCCTCATGT 
     1381 GGGTGGCTGC TGCTATCTGC CTCATTGCCT TTGCCATCCA GGCCAGCGAG GGTGACCTGA 
     1441 CCACTGATGA TAATCTGTAC CTGGCGGTGG CACTCATTGC TGTGGTTGTG GTCACTGGCT 
     1501 GTTTTGGCTA CTACCAGGAG TTCAAGAGCA CAAATATCAT CGCCAGCTTC AAGAATCTTG 
     1561 TACCCCAGCA AGCCACTGTG ATCCGAGACG GGGACAAGTT CCAGATCAAC GCGGATCAGC 
     1621 TTGTGGTGGG CGACCTGGTG GAGATGAAAG GCGGGGACCG CGTGCCAGCA GACATCCGCA 
     1681 TTCTGTCAGC CCAGGGCTGC AAGGTGGACA ACTCCTCGCT CACTGGAGAG TCTGAACCGC 
     1741 AGACCCGCTC GCCCGAGTGT ACACATGAGA GTCCCCTTGA GACCCGCAAC ATCGCCTTCT 
     1801 TCTCCACCAT GTGTCTGGAG GGAACAGCTC AGGGTTTGGT GGTGAGCACC GGCGATCGCA 
     1861 CCATCATTGG GCGCATCGCC TCGCTGGCCT CGGGTGTGGA AAACGAGAAG ACTCCGATTG 
     1921 CAATCGAGAT TGAACATTTT GTGGACATCA TTGCCGGCCT GGCCATCCTC TTCGGTGCCA 
     1981 CATTCTTTGT GGTGGCCATG TGTATTGGCT ATACCTTCCT TCGGGCCATG GTCTTCTTCA 
     2041 TGGCCATTGT GGTAGCCTAT GTGCCTGAGG GGCTGCTGGC AACTGTCACA GTCTGCCTGT 
     2101 CACTGACAGC AAAGAGGTTG GCCAGTAAAA ACTGCGTGGT TAAAAACTTA GAAGCAGTTG 
     2161 AGACCCTAGG TTCTACATCA GTCATCTGCT CAGACAAGAC AGGAACTCTC ACTCAGAACC 
     2221 GCATGACGGT GTCTCATCTA TGGTTTGACA ACCATATCCA CACGGCGGAC ACCACAGAAG 
     2281 ACCAGTCAGG GCAAACGTTC GATCAGTCGT CGGAGACATG GCGAGCGCTG TGCCGCGTGC 
     2341 TCACCCTGTG CAACCGCGCT GCCTTCAAGT CCGGCCAGGA CGCGGTGCCG GTGCCCAAGC 
     2401 GCATCGTGAT CGGAGACGCG TCAGAGACTG CGCTGCTCAA GTTTTCGGAG CTGACGTTGG 
     2461 GCAACGCCAT GGGTTACAGG GACCGCTTCC CCAAAGTTTG CGAGATCCCC TTCAACTCCA 
     2521 TCAACAAGTT CCAGCTGTCC ATTCATACCC TGGAGGATCC ACGCGACTCC CGGCACCTGC 
     2581 TGGTGATGAA GGGCGCCCCT GAGCGCGTGC TGGAGCGTTG CAGCTCCATC CTCATCAAGG 
     2641 GCCAGGAGCT GCCTCTGGAC GAGCAATGGC GTGAGGCCTT CCAGACAGCC TACCTCAGCC 
     2701 TGGGAGGCCT AGGCGAACGT GTTCTTGGTT TCTGCCAGCT CTACCTGAAT GAGAAGGACT 
     2761 ACCCACCTGG CTATGCCTTT GATGTGGAGG CCATGAACTT TCCGAGTAGT GGCCTCTGCT 
     2821 TTGCGGGACT TGTATCCATG ATTGACCCTC CCCGGGCCAC CGTTCCTGAT GCTGTGCTCA 
     2881 AATGCCGCAC GGCAGGCATC CGGGTGATCA TGGTGACCGG TGACCATCCC ATCACGGCCA 
     2941 AGGCCATCGC AGCCAGCGTG GGTATCATCT CAGAGGGCAG CGAGACAGTG GAAGACATCG 
     3001 CCGCCCGCCT CCGGATGCCG GTGGACCAGG TTAATAGGAA GGATGCCCGG GCCTGCGTGA 
     3061 TCAATGGTAT GCAGCTGAAG GACATGGACC CGTCTGAGCT GGTGGAGGCG CTACGCACCC 
     3121 ACCCTGAGAT GGTGTTTGCT CGAACCAGTC CTCAGCAGAA GCTGGGTGCC ATTGTGGCTG 
     3181 TAACAGGGGA TGGTGTGAAT GACTCCCCAG CCCTGAAGAA AGCTGACATT GGTGTAGCCA 
     3241 TGGGCATTGC TGGCTCGGAT GCTGCTAAAA ATGCTGCTGA CATGATCTTG CTGGATGACA 
     3301 ACTTTGCTTC CATTGTGACA GGCGTGGAGC AGGGCCGACT GATCTTTGAC AACCTGAAGA 
     3361 AATCCATTGC ATATACACTG ACCAAGAACA TTCCAGAACT GACACCCTAC CTTATCTATA 
     3421 TCACTGTCAG TGTGCCCCTA CCCCTTGGGT GTATCACCAT TCTCTTCATA GAACTCTGCA 
     3481 CAGATATCTT TCCATCTGTG TCCCTGGCGT ACGAGAAGGC TGAGAGCGAC ATCATGCACC 
     3541 TGCGCCCACG GAACCCTAAG CGTGACCGGT TGGTCAACGA ACCCCTGGCT GCTTATTCCT 
     3601 ATTTCCAGAT TGGTGCCATT CAGTCATTTG CCGGCTTTGC TGACTACTTC ACGGCCATGG 
     3661 CGCAGGAGGG CTGGTTCCCT CTGTTGTGTG TAGGACTGCG GCCACAATGG GAGGACCACC 
     3721 ATCTACAAGA TCTTCAAGAC AGCTACGGCC AGGAATGGAC ATTTGGTCAG CGCCTGTACC 
     3781 AGCAGTACAC CTGTTACACC GTGTTCTTCA TCAGCATCGA GATGTGCCAG ATCGCAGATG 
     3841 TCCTCATCCG CAAGACACGG CGCCTCTCTG TTTTCCAGCA GGGATTCTTC AGGAACAAGA 
     3901 TCCTGGTGAT CGCCATCGTG TTCCAGGTCT GCATTGGCTG CTTCCTGTGC TACTGCCCAG 
     3961 GGATGCCTAA CATCTTCAAC TTCATGCCCA TCCGGTTCCA GTGGTGGCTG GTCCCCATGC 
     4021 CCTTCGGCCT TCTCATCTTT GTCTATGATG AGATCCGGAA ACTTGGAGTT CGCTGTTGCC 
     4081 CAGGGAGCTG GTGGGACCAG GAACTCTACT ATAGCGGACC GACGCGTACG CGGCCGCTCG 
     4141 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4201 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4261 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4321 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4381 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4441 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4501 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4561 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4621 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4681 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4741 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4801 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4861 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4921 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4981 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5041 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5101 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5161 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5221 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5281 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5341 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5401 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5461 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5521 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5581 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5641 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5701 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5761 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5821 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5881 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5941 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6001 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6061 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6121 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6181 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6241 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6301 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6361 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6421 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6481 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6541 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6601 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6661 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6721 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6781 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6841 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6901 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6961 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7021 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7081 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7141 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7201 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7321 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7381 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7441 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7501 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7561 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7621 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7681 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7741 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7801 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7861 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7921 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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