LOCUS       MR221566                8580 bp    DNA     circular  SYN 
DEFINITION  Smc1a (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a)
ACCESSION   NM_019710
VERSION     NM_019710.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4737..4828 
                     /label="Myc-DDK"  
     misc_feature    4858..5443 
                     /label="PolyA signal"  
     misc_feature    4915..4934 
                     /label="Restriction Enzyme"  
     misc_feature    5719..6391 
                     /label="ColE1"  
     misc_feature    6821..7615 
                     /label="Kan/Neo"  
     misc_feature    7650..7952 
                     /label="SV40 ori"  
     misc_feature    8038..8515 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4719..4725 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4719 
                     /label="Smc1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTTTCCTG AAACTGATTG AGATCGAGAA CTTTAAATCG TACAAGGGTC 
     1081 GACAGATTAT CGGACCATTT CAGAGGTTTA CCGCCATCAT TGGACCCAAT GGCTCTGGTA 
     1141 AGTCAAATCT CATGGATGCC ATCAGCTTTG TGCTTGGTGA AAAGACCAGC AACCTGAGGG 
     1201 TAAAGACCCT GCGGGACCTG ATACATGGAG CACCTGTGGG CAAGCCGGCT GCCAACCGAG 
     1261 CCTTTGTCAG CATGGTCTAC TCGGAGGAGG GTGCTGAGGA CCGTACCTTT GCCCGAGTCA 
     1321 TTGTAGGGGG CTCTTCTGAG TACAAGATCA ACAACAAAGT TGTCCAGCTA CATGAATACA 
     1381 GTGAAGAGTT AGAGAAGTTG GGCATTCTCA TCAAAGCTCG TAACTTCCTC GTCTTCCAGG 
     1441 GTGCAGTGGA GTCTATTGCC ATGAAGAACC CCAAGGAGAG GACAGCTCTG TTTGAAGAAA 
     1501 TTAGTCGCTC TGGGGAACTT GCACAGGAGT ATGACAAGCG AAAGAAAGAA ATGGTGAAGG 
     1561 CTGAAGAAGA CACACAGTTT AATTATCATC GCAAGAAAAA TATTGCAGCT GAACGAAAGG 
     1621 AGGCCAAACA AGAAAAGGAA GAGGCGGACC GCTATCAGCG CCTGAAGGAT GAGGTAGTAC 
     1681 GAGCACAGGT ACAGTTGCAG CTCTTTAAAC TCTACCACAA CGAAGTGGAG ATTGAGAAGC 
     1741 TCAACAAGGA ACTGGCCTCG AAGAACAAGG AAATTGAGAA GGACAAGAAG CGGATGGACA 
     1801 AGGTAGAGGA TGAGCTGAAG GAGAAGAAGA AAGAGCTGGG AAAAATGATG CGGGAACAGC 
     1861 AGCAGATTGA GAAGGAGATC AAGGAAAAGG ACTCGGAATT GAATCAGAAG AGGCCTCAGT 
     1921 ACATCAAAGC AAAAGAGAAT ACATCCCACA AAATCAAGAA GCTCGAAGCA GCCAAGAAGT 
     1981 CTCTTCAAAA TGCTCAGAAA CATTATAAGA AACGTAAAGG TGACATGGAT GAGCTGGAGA 
     2041 AGGAGATGCT GTCAGTGGAG AAGGCCCGAC AGGAGTTTGA AGAACGGATG GAGGAAGAGA 
     2101 GTCAGAGTCA GGGCAGAGAC TTGACCCTGG AGGAGAACCA GGTAAAGAAA TACCACCGAT 
     2161 TGAAAGAAGA AGCCAGCAAG AGAGCAGCTA CTTTGGCTCA GGAGCTGGAG AAGTTCAATC 
     2221 GAGACCAGAA AGCTGACCAG GACCGTCTAG ATCTGGAAGA GCGGAAGAAA GTAGAAACAG 
     2281 AGGCCAAGAT CAAGCAAAAA CTGCGGGAGA TTGAAGAGAA TCAGAAACGG ATTGAGAAGC 
     2341 TAGAAGAATA TATTACCACT AGCAAGCAGT CTCTAGAGGA GCAGAAGAAG CTAGAGGGAG 
     2401 AACTGACGGA AGAAGTAGAG ATGGCCAAGC GTCGTATTGA TGAGATCAAT AAGGAGCTGA 
     2461 ACCAGGTGAT GGAGCAATTA GGGGATGCAC GAATTGACCG ACAGGAGAGT AGCCGCCAGC 
     2521 AGCGAAAGGC AGAAATAATG GAAAGCATCA AGCGTCTCTA CCCTGGCTCT GTGTATGGCC 
     2581 GCCTCATTGA CCTCTGCCAG CCTACACAAA AGAAGTATCA GATTGCTGTG ACCAAGGTTT 
     2641 TAGGGAAGAA CATGGATGCT ATTATTGTAG ACTCAGAGAA GACAGGTCGG GACTGTATTC 
     2701 AGTATATCAA GGAGCAACGT GGAGAGCCTG AGACCTTCTT GCCTCTTGAC TACCTGGAGG 
     2761 TGAAACCTAC TGATGAGAAA CTCCGGGAGC TGAAGGGGGC CAAGCTAGTG ATTGATGTAA 
     2821 TTCGTTATGA ACCACCTCAC ATTAAAAAAG CTCTGCAGTA TGCTTGTGGC AATGCCCTTG 
     2881 TTTGTGATAA TGTGGAGGAT GCTCGACGCA TTGCCTTTGG AGGTCACCAA CGCCACAAGA 
     2941 CAGTGGCGTT GGATGGCACC CTGTTCCAGA AGTCAGGAGT TATCTCTGGT GGAGCCAGTG 
     3001 ATCTCAAGGC AAAAGCACGG CGCTGGGATG AGAAAGCAGT AGACAAACTG AAAGAAAAAA 
     3061 AAGAACGTTT GACAGAGGAG CTGAAAGAGC AGATGAAGGC AAAACGGAAA GAGGCAGAAC 
     3121 TGCGTCAAGT GCAGTCTCAG GCCCACGGGC TTCAAATGCG GCTGAAGTAC TCACAGAGTG 
     3181 ACCTAGAACA GACTAAGACA AGGCATCTTG CCCTGAATCT CCAGGAAAAG TCTAAGCTGG 
     3241 AGAGTGAACT AGCCAACTTC GGGCCTCGCA TTAATGATAT CAAGAGAATC ATACAGAGCC 
     3301 GAGAGAGGGA AATGAAAGAC TTGAAGGAGA AGATGAACCA GGTGGAAGAT GAGGTGTTTG 
     3361 AAGAGTTTTG TCGGGAAATT GGTGTGCGTA ACATCCGAGA ATTTGAGGAA GAAAAGGTGA 
     3421 AGCGGCAGAA TGAAATTGCC AAGAAACGCT TGGAATTTGA GAATCAGAAG ACTCGCTTGG 
     3481 GCATCCAATT GGATTTTGAA AAGAATCAGT TGAAGGAGGA CCAGGATAAA GTACACATGT 
     3541 GGGAACAGAC GGTGAAAAAG GATGAAAATG AGATAGAAAA GCTTAAGAAG GAGGAACAAA 
     3601 GACACATGAA GATAATAGAT GAAACCATGG CTCAGCTTCA AGACCTTAAG AATCAGCACC 
     3661 TGGCCAAAAA GTCAGAAGTA AACGACAAGA ATCATGAGAT GGAAGAGATT CGCAAGAAGC 
     3721 TTGGGGGTGC CAACAAGGAA ATGACTCATT TACAGAAGGA AGTGACAGCC ATTGAGACCA 
     3781 AACTTGAACA GAAGCGCAGT GACCGCCACA ACCTGCTACA GGCCTGTAAG ATGCAGGACA 
     3841 TCAAACTGCC ACTGTCAAAG GGCACCATGG ATGATATTAG TCAGGAAGAA GGTAGCTCCC 
     3901 AGGGAGAAGA GTCAGTGAGT GGTTCCCAGA GAACATCCAG TATCTATGCT CGAGAGGCCC 
     3961 TCATTGAGAT TGACTACGGT GACCTGTGTG AAGATCTGAA GGATGCACAG GCGGAGGAAG 
     4021 AAATCAAGCA AGAAATGAAC ACACTCCAGC AAAAGCTGAA TGAGCAGCAG AGTGTGCTTC 
     4081 AGCGGATTGC TGCCCCTAAC ATGAAGGCCA TGGAAAAGCT GGAGAGTGTC CGAGACAAGT 
     4141 TCCAGGAGAC CTCAGATGAG TTTGAAGCAG CACGAAAGCG AGCAAAGAAG GCCAAGCAGG 
     4201 CATTTGAACA GATAAAGAAG GAGCGCTTCG ACCGATTCAA TGCTTGCTTT GAATCTGTGG 
     4261 CTACCAACAT TGATGAGATC TATAAGGCTC TGTCCCGTAA CAGCAGTGCC CAGGCATTCT 
     4321 TGGGCCCTGA GAATCCTGAA GAGCCCTATT TGGATGGCAT CAACTACAAC TGTGTAGCTC 
     4381 CTGGCAAACG TTTCCGGCCT ATGGACAATT TGTCAGGCGG GGAGAAGACA GTTGCAGCTC 
     4441 TGGCCTTGCT TTTTGCCATC CATAGCTACA AGCCAGCCCC CTTCTTCGTC CTGGATGAGA 
     4501 TTGACGCTGC CTTGGATAAC ACCAACATTG GCAAGGTGGC AAATTACATC AAGGAACAGT 
     4561 CAACTTGCAA CTTCCAGGCC ATTGTTATCT CTCTCAAGGA GGAATTCTAC ACCAAGGCTG 
     4621 AGAGCCTCAT TGGAGTCTAT CCTGAGCAAG GGGACTGTGT GATCAGCAAA GTCCTGACTT 
     4681 TCGACCTCAC CAAGTACCCA GATGCCAACC CCAACCCCAA TGAACAAACG CGTACGCGGC 
     4741 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4801 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4861 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4921 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4981 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5041 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5101 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5161 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5221 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5281 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5341 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5401 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5461 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5521 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5581 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5641 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5821 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6241 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6301 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6361 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6421 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6481 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6541 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6601 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6661 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6721 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6781 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6841 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6901 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6961 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7021 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7081 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7141 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7201 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7261 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7321 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7381 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7441 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7501 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7561 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7621 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7681 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7741 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7801 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7981 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8041 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8101 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8221 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8341 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8461 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8521 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//