LOCUS       MR221564                8073 bp    DNA     circular  SYN 
DEFINITION  Kdm4a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 1
ACCESSION   NM_001161823
VERSION     NM_001161823.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4230..4321 
                     /label="Myc-DDK"  
     misc_feature    4351..4936 
                     /label="PolyA signal"  
     misc_feature    4408..4427 
                     /label="Restriction Enzyme"  
     misc_feature    5212..5884 
                     /label="ColE1"  
     misc_feature    6314..7108 
                     /label="Kan/Neo"  
     misc_feature    7143..7445 
                     /label="SV40 ori"  
     misc_feature    7531..8008 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4212..4218 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4212 
                     /label="Kdm4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTGAA TCAGAAACTT TGAACCCCAG TGCTCGGATC ATGACCTTTT 
     1081 ACCCCACCAT GGAAGAGTTC CGGAACTTCA GCCGCTACAT TGCCTACATT GAATCCCAAG 
     1141 GAGCTCATCG GGCTGGGCTA GCCAAGGTCG TTCCTCCCAA AGAGTGGAAG CCTCGAACAT 
     1201 CCTACGACGA TATTGATGAT CTAGTCATCC CTGCGCCCAT CCAGCAGCTG GTGACTGGCC 
     1261 AGTCTGGCCT CTTCACTCAG TACAACATAC AGAAGAAAGC CATGACCGTT CGTGAGTTCC 
     1321 GCAAGATCGC CAATAGCGAC AAGTACTGCA CCCCGCGATA CAGTGAATTT GAAGAACTTG 
     1381 AGCGTAAATA CTGGAAAAAC CTCACATTCA ATCCTCCCAT CTATGGAGCA GACGTGAATG 
     1441 GTACTCTCTA TGAACAGCAC GTTGATGAGT GGAATATCGG CCGGCTGAAG ACCATCCTGG 
     1501 ACCTGGTGGA GAAGGAGAGC GGGATCACCA TTGAGGGGGT GAACACCCCC TACCTTTACT 
     1561 TCGGCATGTG GAAGACGTCC TTCGCCTGGC ACACGGAAGA CATGGATCTA TACAGCATCA 
     1621 ACTATCTGCA CTTCGGAGAG CCAAAGTCTT GGTACTCTGT TCCACCTGAG CATGGGAAAC 
     1681 GCCTGGAGCG TCTTGCCAAA GGCTTTTTCC CAGGAAGTGC TCAAAGCTGT GAGGCTTTTC 
     1741 TCCGCCACAA GATGACCCTG ATCTCCCCAT TAATGCTGAA GAAGTATGGC ATTCCCTTTG 
     1801 ACAAGGTGAC CCAAGAAGCT GGCGAGTTTA TGATCACTTT TCCGTATGGT TACCATGCTG 
     1861 GCTTCAACCA TGGCTTCAAC TGTGCAGAGT CAACCAATTT TGCTACCCGT CGGTGGATTG 
     1921 AGTATGGCAA GCAGGCTGTG CTGTGCTCCT GCAGAAAAGA TATGGTGAAG ATCTCCATGG 
     1981 ACGTGTTCGT GAGGAGGTTC CAGCCGGAAC GCTACAAACT TTGGAAAGCT GGGAAGGACA 
     2041 GCATGGTTAT TGACCACACT CTGCCCACAC CGGAAGCAGC CGAGTTCCTG AAGGACAGCG 
     2101 GCGGACTAAC CCCGAGAGCA GGGAGTGAGG AGTGCCCAGA GGAGGACGTG GAAGCAGCGG 
     2161 ATCAGGGAGA GGAGGGGGAT GTGAAGAGAA GCCTGGCCAA GCATCGGATC GGGACAAAGA 
     2221 GACACAGAGT CTGTCTGGAA ATACCCCAGG AGGTCAGTCA GAGCGAGCTC TTCCCCAAGG 
     2281 AAGAGCTAAG TTCTGGACAG TATGAGATGA CAGAGTGCCC GGCCACCCTC GCCCCAGTGA 
     2341 GGCCCACCCA CAGCTCCGTG CGGCAAGTTG AGGACAGTCT TCCCTTCCCA GATTACTCTG 
     2401 ACCCCACTGA AGTCAAATTT GAAGAGCTGA AGAATGTCAA ACTAGAAGAG GAAGATGAGG 
     2461 AGGATGAGCC CGAAGCAGCC GCACTGGACC TTTCTGTGAA TCCTGCGTCT GTAGGAGGAC 
     2521 GCCTCGTCTT CTCGGGTTCC AAAAAGAAAT CATCTTCCAG CCTGGGCTCC ACTTCATCTC 
     2581 AGGATTCAGT TTCTTCAGAT TCTGAAACCG CCGAGTCTGT CTCTTGCCAG GGCCAAGAGA 
     2641 AAACGGGAGT TCTCACCGTG CACAGCTATG CCAGAGGGGA CGGCAAGGCT GCCACAGGAG 
     2701 AGCCGAGTGT GAAGAAGAAG CGCAGTGCTC CCCGCAGCAT CAGCGAGCAG GAGCTGGCAG 
     2761 AGGTTGCAGA TGAGTACATG CTTTCCCTGG AGGAGAATAA GAAGACCAAG GGTCGCCGCC 
     2821 AGCCCTTGAG CAAGCTCCCA CGCCACCACC CACTCGTGCT GCAGGAGTGC GGCAGCGATG 
     2881 ACGAGACTTC TGAGCAGCTG ACCCCCGAGG AGGAGGCTGA GGAGACAGAG GCTTGGGCCA 
     2941 AGCCTCTGAG CCAGCTGTGG CAGAACCGAC CCCCGAACTT TGAGGCTGAA AAGGAATTCA 
     3001 ATGAGATCAT GGCCCAACAG GCCCCTCACT GTGCTGTGTG TATGATCTTC CAGACCTACC 
     3061 ATCAGGTGGA ATTTGGAGCC TTCAGTCAGA GCTGTGGGGA TGCTTCAGAG CCGGCTGCCC 
     3121 AGACTCAGAG GACCAAGCCA CTGATCCCCG AAATGTGCTT TACCACCACT GGCTGCAGCA 
     3181 CAGACATCAA TCTGTCCACC CCGTACCTAG AGGAGGATGG CACCAGCATG CTAGTGTCCT 
     3241 GCAAGAAGTG CAGTGTCCGG GTCCACGCCA GTTGCTATGG CGTCCCTCCT GCCAAGGCTT 
     3301 CTGAAGAATG GATGTGCTCT CGATGTTCAG CCAATGCCCT GGAGGAAGAC TGCTGCTTAT 
     3361 GCTCGTTGCG TGGAGGGGCC CTGCAGAGAG CCAATGACGA CAGATGGGTT CATGTTTCAT 
     3421 GTGCAGTGGC AATTCTAGAA GCAAGGTTTG TCAACATTGC TGAAAGAAGT CCAGTGGATG 
     3481 TGAGCAAAAT CCCTCTGCCC CGCTTCAAAC TGAAGTGTGT TTTTTGTAAG AAGCGGAGAA 
     3541 AACGGAATGC TGGCTGCTGT GTGCAGTGCT CCCATGGCCG CTGCCCCACT GCCTTCCATG 
     3601 TGAGCTGTGC CCAGGCTGCT GGGGTGATGA TGCAACCCGA CGACTGGCCC TTTGTTGTCT 
     3661 TCATCACGTG CTTCCGTCAC AAGATTCCCA ATCTGGAGCG TGCTAAGGGA GCCTTGCTGA 
     3721 GCATCACGGC GGGCCAGAAG GTTATCAGCA AACACAAGAA CGGGCGCTTC TACCAGTGTG 
     3781 AGGTGGTCAG ACTCACCACC GAGACCTTCT ACGAAGTCAA CTTTGACGAC GGCTCCTTCA 
     3841 GTGACAACCT TTATCCTGAG GACATAGTGA GTCAGGACTG TCTCCAACTG GGGCCTCCTG 
     3901 CTGAAGGTGA AGTGGTTCAA GTCAGATGGA CAGATGGCCA AGTCTATGGG GCTAAGTTTG 
     3961 TGGCCTCCCA CCCCATCCAG ATGTACCAGG TGGAGTTTGA GGATGGCTCG CAGCTGGTGG 
     4021 TTAAGAGAGA TGATGTATAC ACACTCGATG AAGAGCTTCC CAAGAGAGTC AAGTCTAGAC 
     4081 TGTCGGTGGC TTCAGACATG CGCTTCAATG AGATTTTCAC AGAGAAGGAA GTGAAGCAAG 
     4141 AAAAGAAGAG GCAACGGGTC ATCAACTCGC GGTACCGGGA GGATTACATC GAGCCAGCGC 
     4201 TGTACCGGGC CATCATGGAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4261 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5881 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5941 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6001 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6061 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6121 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6181 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6241 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6301 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6361 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6421 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6481 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6541 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6601 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6661 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6721 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6781 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6841 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6901 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6961 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7021 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7081 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7141 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7201 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7261 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7321 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7501 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7561 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7621 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7681 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7741 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7801 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7861 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7921 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7981 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8041 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//