LOCUS       MR221499                7296 bp    DNA     circular  SYN 
DEFINITION  Hps6 (Myc-DDK-tagged) - Mouse Hermansky-Pudlak syndrome 6 (Hps6)
ACCESSION   NM_176785
VERSION     NM_176785.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3453..3544 
                     /label="Myc-DDK"  
     misc_feature    3574..4159 
                     /label="PolyA signal"  
     misc_feature    3631..3650 
                     /label="Restriction Enzyme"  
     misc_feature    4435..5107 
                     /label="ColE1"  
     misc_feature    5537..6331 
                     /label="Kan/Neo"  
     misc_feature    6366..6668 
                     /label="SV40 ori"  
     misc_feature    6754..7231 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3435..3441 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3435 
                     /label="Hps6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCGTGCA GGAACTCTGC GCCTGCTTTC GGATCTGAGC AACTTCACCG 
     1081 GCGCGGCCCG GCTCCGCGAG TTGTTGGCGG GGGACCCAGC TGTCCTAGTC CGCTGCAGCC 
     1141 CTGACGGCCG CCACTTGCTG CTATTAAGAC CCCCCGGATC GCCCGCCCCA CAGCTTCTAG 
     1201 TGGCTGTGCG TGGGCCCGGC CTGCCGCTGG AGCGTGCCTG GCCGGAGGGC GACCCCTCGC 
     1261 CGCTGGACGT CTTTTTCGTG CCGTGGCTGG CGCGACCCGC GCTGATCTTG GTATGGGAGA 
     1321 GTGGCCTAGC AGAGGTTTGG GGCGTGGGGA TGGAGCCTGG ATGGAAGCTA CTTCAGAGCA 
     1381 CTGAGCTGTG TCCGGATGGT GGAGCCCGCG TGATGGCCGT GGCCGCAACC CGAGGCCGCC 
     1441 TAGTTTGGTG CGAGGAGCGT CAGCCGGGTG TTAAGGACCA ACCAGAGCAG CTTTCAACGG 
     1501 CCTTCAGCCA CCGTGTGTGC TTCAAGACCC TGGAAACCAG CGGGGAGGCT GGCACCAAAC 
     1561 TAGGCTGCAC CCACATCCTG CTACACCATT GCCCCCTTTT TGGACTGATA GCCTCCCGCA 
     1621 AGGACCTCTT CCTGGTGCCT ACTACCAACA CTTGGTCTGG TGTGGCCCAC CTTCTGCTCA 
     1681 TCTGGAGCCC AAGCAAGGGG AAGGTAATAG TTGCTGCTCC ATCTCTTGGT CTTTCACACA 
     1741 GTAAAAGCCT GAATCCCAAA CAAGGGGACA CTTGGGACTT CCGGACCCTG CTGCGAGGCC 
     1801 TTCCTGGATT CCTGTCCCCC AGGGAGCCAC TGGCTGTACA CACTTGGGCC CCATCTTCGC 
     1861 AGGGCTTGTT GTTGCTTGAC TTGAAAGGGA AGGTGAGCCT AGTGCAGTGC CATGGTGGTA 
     1921 CTCGAACCGT GGGAATCCTG CAGGAGGCCC CTGTAAGCCT AAAAGGGTCT GCAGCCCTGG 
     1981 GGACATTTCA CGGCACTTTA GCCTGTGTCC TGGGCTCCAC CTTGGAACTA CTGGACATGA 
     2041 GCAGTGGGCG GCTGTTGGAA AAAAAGGTTC TCAGTACAGA CAGAGTACAT TTGCTGGAGC 
     2101 CTCCAGCCCC GGGCATGAAG AACGAGGAAG AGCTGGAGAC CCGAGGAGCC CTACGATTGC 
     2161 TTTCAGCCTT GGGTCTCTTT TGTGTGTGTT GGGAAACTCC CCAAGGCCTT GAGCTGCCTT 
     2221 CAGACAAGGA CCTGGTGTTT GAGGAGGCCT GTGGGTACTA CCAGCGTCGG AGCCTTCGAG 
     2281 GTACCCAGCT TACCCCAGAA GAACTGAGAC ACAACAGCAT GTTTCGGGCA CCTCAGGCCC 
     2341 TGGCCTCCAT CCTCCAGGGC CACCTGCCCC CGTCTACACT CCTGACAACC CTGAGGGCTG 
     2401 AGCTCCGGGA TTATCGTAGT ATAGAACAGC TCAAGGCCCA GCTGGTGGCT GGGGACGATG 
     2461 AGGAGGCTGG CTGGACTGAG TTAGCGGAGC ACGAGGTGGC CCGCCTGCTG AGAACCCAGT 
     2521 TGACAGGAGA CCAGCTGGCC CAATTCAACA CCATTTTCCA AGCCCTTCCT ACAGCAGCTT 
     2581 GGGGTGCCAC CCTCCAGGCA CTGCAGCTCC AGCCAGATAG GAGTGGCAGG CTGAGGTCCC 
     2641 AAGCACCCCC GGATGTATGG AAGAAGGTCC TAAGGGCTCC AACAGCTGGA AAGGAACATC 
     2701 CCAATGGAAT ACTGCCGCCC TTTGAACTCC TGTGTCAGTG CCTGGGCCAG CTCGAGCCTC 
     2761 AGTGGCTCCC CCCATTTGTG AAACTGGCAC AGCAGCAGGG TGGCCCCGGC TGGGGGGCTG 
     2821 AAGGCCCCAG TCTGCCCCTT TACCGCCGAG CCCTGGCAGT GCTAGGTGAG GAAGGGAAGA 
     2881 GGCCTGAGGC TCTGGAACTC GAGTTGCTTT TGGGCAGTGG GCGACCCAAA GCCGTGCTAC 
     2941 AAGCTGTAAG GCAGCTGATA AAGAAAGAAG AGTGGGAACG GGCTCTGGAA GCCGGGCTGG 
     3001 CCCTGGATGC CTCCAGCCCC CTGCTTCGAA GTGAGATCTT TAAGCTGCTG CTGGCAGAGT 
     3061 TTGCCCAGCA CCGCCGCCTG GATGCACACC TCCCTCTCCT CTGTCGCCTG TGCCCACCGG 
     3121 AAGTGGCTCC ACATGAGCTC CTACTTCTGC TGAGGACACA TCTCCCAGAT GATGAGGGAA 
     3181 CCACCCCTTT CCCTGAGCCT GGGGCAGAGC CCCCTCTCAC AGTGGGCTTG GTCAGAGCCC 
     3241 TGCTAGAACA GACTGGGGCT CAAGGAAGGC CGTCTGGCCC AGTTCAAAGC ACCTATGAGG 
     3301 ACATCTTATG GGACCCAGGC ACTCCACCCC CTACACCACC TCGTGGACCC ACTGCTTCTC 
     3361 TCCCGGCATC AGACCATCCA GGACAAGAAG CCTGGGTACC ACCTGGACAA GGGCTCGGTG 
     3421 CAGCTGATGT GGGAGTTCAT TTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3481 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3541 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3601 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3661 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3721 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3781 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3841 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3901 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3961 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4021 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4081 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4141 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4201 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4261 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4321 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4381 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4441 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4501 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4561 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4621 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4681 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4741 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4801 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4861 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4921 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4981 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5041 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5101 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5161 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5221 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5281 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5341 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5401 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5461 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5521 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5581 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5641 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5701 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5761 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5821 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5881 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5941 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6001 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6061 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6121 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6181 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6241 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6301 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6361 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6421 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6481 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6541 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6661 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6721 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6781 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6841 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6901 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6961 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7021 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7081 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7141 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7201 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7261 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//