LOCUS       MR221477                8934 bp    DNA     circular  SYN 
DEFINITION  Gpr116 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 116 (Gpr116)
ACCESSION   NM_001081178
VERSION     NM_001081178.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5089..5180 
                     /label="Myc-DDK"  
     misc_feature    5210..5795 
                     /label="PolyA signal"  
     misc_feature    5267..5286 
                     /label="Restriction Enzyme"  
     misc_feature    6071..6743 
                     /label="ColE1"  
     misc_feature    7173..7967 
                     /label="Kan/Neo"  
     misc_feature    8002..8304 
                     /label="SV40 ori"  
     misc_feature    8390..8867 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5064..5071 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5064 
                     /label="Adgrf5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGATCGCCA AGGACCTTCA CGTTCTACTT CCTGCTTCTT GTGATTTGTT 
     1081 CTTCAGAAGC TGCGCTGAGC ACACCAACAG AGCCCATTGT TCAACCTTCA ATTCTCCAAG 
     1141 AACATGAGCT AGCCGGGGAA GAGCTACTGA GGCCAAAAAG AGCAGCTGCA GCGGGTGATC 
     1201 GCGTGGCGGA AGAATACATG GTGGATATTG AGATCAGCTT TGAAAATGTC TCCTTCCTGG 
     1261 AGTCCATCAG AGCTCACTTG AACAACCTCA GTTTTCCAAT TCGAGGGACC GAAGCTGACA 
     1321 TTTTGAACAT AGCAATGACT ACAGTCTGCA CTCCTGCTGG AAATGACCTC TTGTGCTTCT 
     1381 GTGAGAAAGG CTACCAGTGG TCTGAGGAAA GGTGTCTCCA CTCTCTCACT TGTCAAGACT 
     1441 ATGACAGTGC CCTGCCAGGC GGCTACTGCA GTTGTCTGAA AGGACTTCCT CCCCAGGGAC 
     1501 CTTTCTGCCA GCTCCCAGAA GCATTCATTA CCTTAAAACT CAAAGTCAGA CTGAACATAG 
     1561 GATTTCAAGA AGACCTAAAG AACACGTCTT CTGCCCTCTA TAGGTCCTAC AAGACTGATC 
     1621 TGGAAAGAGC GTTCCGAGCG GGTTACAGAA CTTTACCAGG ATTCAGATCG GTGACTGTGA 
     1681 CACAGTTCAC CAAGGGCAGC GTGGTTGTGA ACTACGTAGT CCGGGTTACA TCGGCACCAC 
     1741 TGCCTGGATC TATTCATAAA GCCAATGAAC AAGTCATACA GAACCTCAAC CATACTTACA 
     1801 AAATGGACTA CAACTCCTTC CAGGGAACCC CGAGCAATGA GACGAAATTC ACTGTCATAC 
     1861 CAGAATTCAT CTTTGAGGGA GACAATGTGA CTCTGGAGTG TGAAACTGAG TTCGTGACCT 
     1921 CCAACACGTC TTGGTACTAT GGAGAAAAAC GCTCTGACAT CCAGAACAGC GACAAATACT 
     1981 CCATTCACAC CACTGTCATC AACAATATAA GCCTGATCAC CCGCCTTACC ATTTACAACT 
     2041 TCACACAGCA TGATGCGGGC ATGTATGGTT GCAATGTGAC ATTGGATATT TTTGAATATG 
     2101 GAACAGTCAG AAAATTAGAT GTCACTCCCA TCCGGATTTT GGCCAAGGAG GAGAGAAAGG 
     2161 TGGTGTGTGA CAACCATCCT ATATCGCTGA ACTGCTGCAG CGAGAATATT GCAAACTGGA 
     2221 GCAGTATTGA ATGGAAACAG GAAGGGAAGA TAAGCATTCT AGGGAACCCA GAATCAGACC 
     2281 TAGAGTCTAG CTGCAGCACT TACACGCTCA AGGCAGATGG AACCCAGTGT CCCAGTGGGT 
     2341 CTTCTGGAAC AACAGTCATC TACACATGCG AGTTCGTCAG TGCCTATGGA GCCAGAGGCA 
     2401 GTAAGAACAT AGCTGTGACC TTCACCTCTG TAGCCAACCT AACAATAACC CGGGACCCAA 
     2461 TTTCTGTTTC TGAGGGACAA AGCTTTTCTA TAACCTGCCT CAGCGATGTG AGTAGCTTTG 
     2521 ATGAGGTGTA CTGGAACACC TCAGCTGGTA TTAAAATCCA CCCAAGGTTT TATACCATGA 
     2581 GGAGGTATCA GGATGGAGCA GAGTCGGTGC TGATGGTGAA GACCTCCACC CGAGAGTGGA 
     2641 ACGGAACCTA CCACTGCATA TTTAGATATA AGAATTCATA CAGCATTGCC ACCAAAGATG 
     2701 TCACCGTTCA CCCGCTGCCT CTGGTGTCAG ACATCATGAT GGATCCTTTG GAAGCCAGTG 
     2761 GTCTGTGTAC CAGTTCCCAT CAATTCAAAT GCTGCGTAGA AGAGGATGCA GGGGAAGAGT 
     2821 ACGCTGTGAC TTTCCACGTG GACTCCTCAT CCTTTCCCGC TGAAAGAGAA GTTATCGGCA 
     2881 AGCAAGCATG CTACACATAC AGTCTCCCAG CGAACTTACC TCGATGTCCC AAAGACATCG 
     2941 CAGTGTTCTG CCACTTCACC AATGCAGCCA ACAGCTCAGT CCGGAGCCCA TCTATGAAGC 
     3001 TCAAACTTAT TCCAAGGGAG AATGTCACAT GCCAGGATCC CATCATTGGC ATTGGGGATC 
     3061 CTGGGAAAGT CATTCAGAAG CTGTGCCAGT TCTCAGGTGT TTACGGAAGC CCTGGACAGG 
     3121 CCATCGGCGG GACTGTCACT TACAAATGTG TGGGCACCCA GTGGAAGGAG GAGTCAAGGG 
     3181 CCTGCATCTC AGCTCCAATC AATGGTTTGC TCCAGGTGGC CAAGGCTTTG ATCAAGAGCC 
     3241 CCACCCAGGA CCAGAAGCTC CCTACATACC TGAGGGACCT TTCTGTTAGT GCTGGGAAGG 
     3301 AAGAACAGGA CATCCGCTCA TCTCCAGGAA GCCTGGGAGC CATCATCAGC ATCCTTGACT 
     3361 TGCTCTCAAC GGTTCCCACC CAAGTGAATT CAGAAATGAT GAGAGACATA CTTGCTACCA 
     3421 TTAACGTCAT CTTAGACAAG TCTGCCTTGA ACTCCTGGGA GAAGTTGCTA CAGCAACAGA 
     3481 GCAATCAGAG TTCTCAGTTC CTGCACTCCG TGGAAAGGTT TTCCCAAGCC CTCCAGCTGG 
     3541 GAGACAGCAC TCCTCCATTC CTCGCCCACC CTAACGTTCA GATGAAGAGC ATGGTGATAA 
     3601 AACGTGGCCA CCCCCAAATC TACCAACAGC AGTTTATCTT CAAAGACTCT GACCTGTGGG 
     3661 GTGATGTAGC CATTGATGAG TGTCAGCTGG GAAACTTGCA GCCTGATTCA TCGATCGTTA 
     3721 CTGTGGCTTT CCCAACTCTC AAAGCCATCC TTGCCCAGGA TGTACAGAGA AAGACATCCT 
     3781 CCAACAGCTT AGTAATGACA ACCACTGTCA GCCATAACAT TGTCAAGCCA TTTAGGATTT 
     3841 CTATGACTTT TAAGAACAAC CATCGTTCGG GTGGCAAGCC ACAGTGTGTC TTCTGGAACT 
     3901 TCAGTCTTGC CAACAATACA GGGGGCTGGG ACAGCAGTGG GTGCTCTGTG GAAGATGATG 
     3961 GTAGGGACAA TAGGGACAGA GTCTTCTGCA AGTGTAACCA CCTGACGTCA TTCTCCATCC 
     4021 TCATGTCCCC AGACTCCCCA GATCCAGGGT CTCTTTTAAA AATACTTCTG GATATCATTT 
     4081 CTTACATCGG GTTGGGGTTT TCCATAGTCA GCTTAGCTGC CTGTCTAGTT GTGGAAGCCA 
     4141 TGGTGTGGAA ATCTGTGACC AAGAACCGAA CTTCCTATAT GCGCCACATC TGCATAGTCA 
     4201 ACATTGCCTT TTGCCTTCTG ATTGCTGACA TCTGGTTCAT TGTGGCTGGT GCTATCCACG 
     4261 ACGGTCGCTA CCCACTCAAC GAAACAGCCT GTGTGGCCGC CACATTCTTC ATTCACTTCT 
     4321 TCTACCTCAG TGTCTTCTTC TGGATGCTAA CTCTAGGCCT CATGCTCTTC TACCGGCTGA 
     4381 TTTTCATTCT ACACGATGCA AGCAAGTCCA CTCAGAAAGC CATCGCATTT TCTCTAGGCT 
     4441 ATGGCTGTCC CCTCATTATC TCCTCTATCA CAGTGGGGGT TACGCAGCCA CAGGAAGTCT 
     4501 ACATGAGGAA GAACGCGTGT TGGCTCAACT GGGAGGACAC CAGAGCACTG CTGGCTTTTG 
     4561 CCATCCCCGC GTTGATTATT GTGGTGGTAA ATGTGAGCAT CACAGTTGTG GTCATCACCA 
     4621 AGATCCTGAG GCCCTCCATT GGGGACAAGC CAGGCAAGCA AGAGAAGAGC AGCCTATTCC 
     4681 AGATCAGCAA GAGTATCGGG GTCCTCACAC CACTCTTGGG GCTCACTTGG GGTTTCGGTC 
     4741 TTGCCACAGT GATCCAGGGA AGCAATGCTG TGTTCCACAT CATATTTACT CTCCTCAATG 
     4801 CCTTCCAGGG GCTCTTCATT TTGCTCTTTG GCTGCCTCTG GGATCAGAAG GTGCAGGAAG 
     4861 CTTTGCTGCA TAAGTTTTCA TTGTCAAGGT GGTCTTCTCA ACACTCAAAG TCAACATCCA 
     4921 TAGGTTCGTC AACACCCGTG TTTTCAATGA GTTCTCCAAT ATCCCGAAGA TTTAATAATC 
     4981 TTTTTGGTAA AACAGGAACA TATAACGTTT CCACCCCAGA GACAACCAGT TCATCCCTGG 
     5041 AAAATTCCTC TAGTGCTTAC TCACTGCTCA ACAGCGGACC GACGCGTACG CGGCCGCTCG 
     5101 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5161 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5221 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5281 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5341 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5401 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5461 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5521 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5581 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5641 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5701 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5761 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5821 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5881 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5941 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6001 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6061 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6121 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6181 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6241 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6301 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6361 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6421 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6481 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6541 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6601 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6661 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6721 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6781 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6841 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6901 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6961 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7021 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7081 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7141 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7201 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7261 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7321 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7381 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7441 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7501 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7561 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7621 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7681 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7741 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7801 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7861 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7921 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7981 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8041 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8101 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8161 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8221 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8281 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8341 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8401 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8461 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8521 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8581 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8641 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8701 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8761 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8821 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8881 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//