LOCUS       MR221394                8151 bp    DNA     circular  SYN 
DEFINITION  Fuk (Myc-DDK-tagged) - Mouse fucokinase (Fuk), transcript variant 1
ACCESSION   NM_172283
VERSION     NM_172283.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4308..4399 
                     /label="Myc-DDK"  
     misc_feature    4429..5014 
                     /label="PolyA signal"  
     misc_feature    4486..4505 
                     /label="Restriction Enzyme"  
     misc_feature    5290..5962 
                     /label="ColE1"  
     misc_feature    6392..7186 
                     /label="Kan/Neo"  
     misc_feature    7221..7523 
                     /label="SV40 ori"  
     misc_feature    7609..8086 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4290..4296 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4290 
                     /label="Fuk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGTCA GAGGGAGTCA ATTGGACTGT CATTATCCTG ACATGCCAGT 
     1081 ACAAGGACAG TGTCCAGGTC TTTCAGAGAG AGCTGGAGGT AAGGCAGAGA CGGGAGCAGA 
     1141 TTCCTGCGGG GACGATGTTA CTGGCTGTGG AGGATCCCCA GACTCGAGTC GGCAGCGGAG 
     1201 GAGCCACCCT CAACGCACTG CTGGTGGCTG CTGAACACTT GAGTGCCCGA GCTGGCTTCA 
     1261 CTGTGGTCAC GTCCGATGTC CTGCACTCTG CCTGGATCCT CATCTTGCAC ATGGGCCGAG 
     1321 ACTTCCCCTT CGATGACTGT GGCAGGGCCT TCACTTGCCT CCCTGTGGAG AACCCACAGG 
     1381 CCCCTGTGGA GGCCTTGGTA TGCAACCTGG ACTGCCTGTT GGATATCATG ACCCACCGGC 
     1441 TGGGTCCAGG TTCCCCACCA GGTGTGTGGG TCTGCAGCAC CGACATGCTT CTGTCTGTTC 
     1501 CTCCAAACCC TGGGATCAGT TGGGATGGCT TCCGGGGAGC CAGAGTGATC GCCTTTCCTG 
     1561 GGAGCCTGGC CTATGCGTTG AACCACGGTG TCTACCTCAC TGACTCACAG GGCTTGGTTT 
     1621 TGGACATTTA CTACCAGGGC ACTAAGGCGG AGATACAACG TTGTGTCGGA CCTGATGGGC 
     1681 TGGTACCATT GGTCTCCGGG GTCGTCTTCT TCTCTGTGGA GACTGCTGAG CACCTCCTAG 
     1741 CCACCCATGT GAGCCCACCG CTGGATGCCT GCACCTATAT GGGCTTGGAC TCTGGAGCCC 
     1801 AGCCTGTGCA GCTGTCTCTG TTTTTCGACA TCCTGCTCTG CATGGCTCGG AATATGAGCA 
     1861 GGGAGAACTT CCTGGCTGGG CGGCCCCCGG AGTTGGGGCA AGGTGACATG GATGTAGCAA 
     1921 GTTACCTGAA GGGAGCCCGG GCCCAGCTGT GGAGGGAGCT TCGAGATCAG CCCCTCACAA 
     1981 TGGTGTATGT CCCTGACGGC GGCTACAGCT ACATGACGAC TGATGCCACC GAGTTCCTGC 
     2041 ACAGACTCAC GATGCCTGGA GTAGCTGTGG CACAGATTGT TCACTCCCAG GTGGAGGAGC 
     2101 CACAGCTGCT AGAGGCTACG TGCTCGGTGG TCAGCTGCCT GCTCGAGGGC CCTGTGCACC 
     2161 TGGGGCCTCG AAGTGTCCTG CAGCACTGTC ACCTGAGGGG CCCCATTCGC ATCGGCGCTG 
     2221 GCTGCTTTGT GAGTGGTCTG GATACAGCCC ACTCGGAGGC ACTGCATGGC CTGGAGCTCC 
     2281 ATGATGTCAT CCTGCAGGGA CACCATGTGC GGCTGCATGG CTCCCTGAGC CGTGTATTTA 
     2341 CTCTTGCTGG CCGTCTGGAC AGCTGGGAAA GACAGGGGGC AGGCATGTAT CTCAACATGT 
     2401 CCTGGAATGA GTTCTTCAAG AAGACAGGCA TTCGAGACTG GGACCTGTGG GACCCAGATA 
     2461 CACCCCCCTC AGATCGATGC CTCCTCACTG CCCGCCTTTT CCCTGTGCTC CACCCCACGA 
     2521 GGGCCCTGGG GCCCCAGGAT GTGCTGTGGA TGCTGCACCC CCGCAAACAC AGAGGTGAGG 
     2581 CCCTTCGGGC CTGGCGAGCC TCCTGGCGTC TGTCCTGGGA GCAGCTGCAA CCTTGTGTGG 
     2641 ACCGGGCTGC CACACTGGAC TTCCGCCGAG ATCTGTTCTT CTGCCAGGCC TTGCAGAAGG 
     2701 CAAGGCATGT GTTAGAGGCG CGGCAGGACC TCTGCCTACG TCCACTGATC CGGGCCGCTG 
     2761 TCGGGGAAGG TTGCTCTGGG CCCCTGCTGG CCACACTTGA CAAGGTTGCA GCTGGGGCAG 
     2821 AAGATCCTGG CGTGGCAGCC CGGGCTCTGG CTTGTGTGGC CGATGTGCTG GGCTGCATGG 
     2881 CAGAGGGCCG AGGAGGCTTG CGCAGTGGGC CAGCTGCCAA CCCTGAGTGG ATTCAGCCTT 
     2941 TCTCATACTT GGAGTGTGGA GACCTGATGA GGGGTGTGGA GGCGCTTGCC CAGGAGAGAG 
     3001 AGAAGTGGCT GACCAGGCCT GCCTTGCTGG TTCGAGCTGC CCGCCATTAC GAGGGGGCCG 
     3061 AGCAGATCCT GATCCGCCAG GCTGTGATGA CAGCCCGGCA CTTCGTCTCC ACCCAGCCCG 
     3121 TGGAGCTGCC CGCACCCGGG CAGTGGGTGG TGACTGAGTG CCCAGCCCGT GTGGATTTCT 
     3181 CTGGGGGCTG GAGTGACACA CCGCCCATTG CCTATGAGCT TGGTGGAGCA GTGTTGGGCC 
     3241 TGGCTGTGCG GGTGGATGGC CGCCGGCCCA TCGGGGCCAA AGCACGCCGC ATCCCGGAGC 
     3301 CTGAGCTCTG GCTGGCAGTG GGACCTCGGC AGGATGAGAT GACCATGAGG ATAGTGTGCC 
     3361 GGAGCCTGGA TGACCTGCGG GATTACTGCC AGCCTCATGC CCCAGGGGCC TTGCTGAAGG 
     3421 CAGCCTTTAT CTGTGCTGGC ATTGTGCATC TCCACTCAGA GCTCCCTCTG CTTGAACAGT 
     3481 TGTTACACTC CTTTAATGGT GGCTTTGAGC TGCACACGTG GTCAGAGCTG CCGCACGGCT 
     3541 CTGGTCTTGG CACCAGCAGC ATCCTGGCAG GGGCTGCCCT GGCTGCCTTA CAGCGGGCTG 
     3601 CAGGCCGGGC AGTGGGCACG GAGGCTCTCA TCCACGCAGT GCTGCACCTG GAGCAGGTGC 
     3661 TCACCACAGG AGGTGGCTGG CAGGACCAAG TCAGTGGCCT AATGCCTGGC ATCAAAGTGG 
     3721 GGCGCTCCCG GGCCCAGCTG CCCCTCAAGG TGGAGGTGGA GGAAATCACT GTGCCTGAGG 
     3781 GCTTTGTCCA GAAGATCAAT GACCATCTGC TCCTGGTTTA TACCGGCAAG ACCCGATTGG 
     3841 CCCGGAATCT GCTGCAGGAC GTGCTGAGGA ACTGGTACGC TCGGTTGCCC GTTGTGGTAC 
     3901 AGAATGCCCG CAGACTGGTG CGACAGACCG AGAAGTGCGC TGAAGCTTTC CGCCAAGGAA 
     3961 ACCTGCCTCT GCTGGGACAG TACCTGACCT CATACTGGGA GCAGAAGAAG CTTATGGCCC 
     4021 CAGGCTGCGA GCCGCTGGCC GTGCAGCGAA TGATGGATGT CCTGGCCCCG TATGCGTATG 
     4081 GCCAAAGCCT GGCAGGGGCA GGTGGTGGGG GCTTTCTCTA TCTATTGACC AAGGAACCCC 
     4141 GGCAGAAAGA GACTCTGGAA GCTGTCCTGG CCAAGGCTGA GGGCCTTGGC AACTACAGTG 
     4201 TCCACCTGGT GGAAGTGGAT CCTCAGGGCC TGAGCCTGCA GCTGCTGGGA CACGACACCC 
     4261 GTCTTTGTGG GGCCGGGCCC TCTGAAGTGG GCACCACCAC GCGTACGCGG CCGCTCGAGC 
     4321 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4381 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4441 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4501 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4561 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4621 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4681 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4741 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4801 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4861 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4921 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4981 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5041 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5161 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5221 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5281 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5341 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5461 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5521 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5581 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5641 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5761 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5821 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5881 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5941 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6001 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6061 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6121 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6181 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6241 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6301 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6361 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6421 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6481 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6541 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6601 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6661 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6721 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6781 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6841 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6901 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6961 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7021 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7081 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7141 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7201 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7261 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7321 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7381 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7561 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7621 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7681 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7741 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7801 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7861 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7921 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7981 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8041 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8101 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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