LOCUS       MR221359                8814 bp    DNA     circular  SYN 
DEFINITION  Map3k19 (Myc-DDK-tagged) - Mouse Yeast Sps1/Ste20-related kinase 4 (S. cerevisiae) (Ysk4)
ACCESSION   NM_011737
VERSION     NM_011737.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4971..5062 
                     /label="Myc-DDK"  
     misc_feature    5092..5677 
                     /label="PolyA signal"  
     misc_feature    5149..5168 
                     /label="Restriction Enzyme"  
     misc_feature    5953..6625 
                     /label="ColE1"  
     misc_feature    7055..7849 
                     /label="Kan/Neo"  
     misc_feature    7884..8186 
                     /label="SV40 ori"  
     misc_feature    8272..8749 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4959 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="Map3k19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTCTATG CCCAGACCAG AAAGACATGC CGAGTCACTG CTTGACATCT 
     1081 GTCATGAGGC AGGCTCTACT CCAATGGAGA TGACAGTTTC CCAAACTGAA AACCTCACCT 
     1141 TGCAGAGCAT CAGCAGCAGT GAGGACTTTG ACCTTGAAGA TGACTTCAGC CCGTTCATAT 
     1201 TGGCTAGGGA AGGAGCAGCT CCCAGGGGTG AAAACTGGCC ACGAAGGACA GAAGGTGTGG 
     1261 AGATTATTGT GACGTTTCCA CCGGATCCTC TCCAAGAAGC AAGCCAAGAA GACTTGAAAG 
     1321 AAAGCAATCA GGTGACTTCA GAGCATCAGG AACGGGAACA AGTGCATCCT GTCTCTCCAC 
     1381 CAGATGACGC GGAGATGGTG GGCCTCACGG GGAGGATGCT AACCACACAG CCTTCACTTC 
     1441 TGAAGACAGA CGGAAGTTCT GAGGAGCTCT GTGGTGTGGA CGTGGCTCTC TCACCACCCA 
     1501 GACCTTGCTT AGACTCAAGC CTTGCTGCAT CTGCAGCTGG GGAGGTGGCT CCTTGTGTTC 
     1561 TGAAAGAACA GCAAAGGCAA TCTGATGAGT TTCCTACCTC TGACATAAGC TACAGTAGCC 
     1621 GAAGAACTGG GCTTCCTCTG CCACCACTAT CATGCTTGCC CATGAGATCT TGTATCTTCA 
     1681 ATATGGAAAA AAGCCCCAAG TCCCCAAGGC ACAGGGAAAG AAAGGTCCCC AGCCTCAGCC 
     1741 TGTCTGTGCC TAAGCTCCTG GAACCTCTTA GCCGACCTCT TAGCCAATCT GCAGAGTTTA 
     1801 GTTCATCAAA GAACCATCAA GAAGTCACGC AGGAAGGACC AGTGGAACAC ACCCTCAGGG 
     1861 GTTCTAACTG TACCCTCTGG TCCAGAAACA TGTGTTCTTT CCGGAAGAGC GGAAAACAGG 
     1921 GAGTAGCGGA GAGTTGGCCA TCCCAGGAAA TGGAAGGGTG GGACAAAACT AAAACCTCTG 
     1981 GCTTCAAAGA AGGGCCATCT TTGTTCTCTT GTGAGAGTGT CAAAGAAGAC ACTACTCCTA 
     2041 CAGAGAGGGA AAGGGATAGT GGGTACCATG TTAGTGAGAT GCAAAGAGGG GGAGAGGACT 
     2101 CCCAATATCT TTCATCAAGA AAAAAAGAGA GTTGGACAGC CAGAGTGGTT GAGCGAGATT 
     2161 CAGGGGTTGA GCACCCCATT CTCTGCAAGC TCCTGGAAGT CTCAAATTCA GAAATGACCC 
     2221 CAGCAGAGGA GAAAGAGATC GGAAATGAGA ACGTTCCTGA TGCCAAAAGC AATTCTGTAC 
     2281 ACAAATCTGG AGCAATGGAG CCACATGCAG CGTCAGAAGA GGTTAGTGTT CCTAAGAATG 
     2341 GTCCCAGTGT GAACTCTGAC GGCCCTGCTG AAGAGCTGGA AGGTCATAGA GACATAGAAC 
     2401 AGAACAGAAA AATACCAATG GAGGAAGAAA CAAATCCCGA AATGAATGGG GTGGTGCCGC 
     2461 TTACTCACAT CGCATTCCCT GGAGAAGGGA CATCCAAGGG ACCCGCTAGA GCCGAACCAC 
     2521 ACCTCCAAAG GAGAAAGCGC CCGGCGCAGA ACAGCAACAG CTTCAACCTG CTTGCTCACC 
     2581 GAGAACACGA CAAACTCCAG ACAAATACTC ATAGAACCAA GTTGGATTCA AGGACCAAGG 
     2641 CCAGGAACAG AGCACCCCCT AATCTCATGG TTTCCATCCA AGCATCCATT AAGCCCAACA 
     2701 TGCATAAAAA CAGCATAAAA ACTCAAGTTT TCCCTGCTTT GGAACTTATA GACCACAGAC 
     2761 CTCATCCCTC CTCCAAGTTT CAAAGGAGAG CACCTCTGAC AGAAAAGAAG TCAACGCATC 
     2821 AGACCCAGAA ACCTAAAAAG CAAGCATTTC CTCGAATCGG TAAACATGCA GGCATAAAAA 
     2881 AGCCAGGCAT TCCCCTCTCC GCTGAAACAA CAGATCCGAG ACTGCACTTC CTGGATTTGA 
     2941 AGTACAGTGA CATGTTCAAA GAAATCAACT CAGCCTCCAA CGGACCAGGC ATCTATGAGA 
     3001 TGTTTGGAAC CCCTGTTTAT TGTCACATTC GCGAGGCAGA AAGGCACGAC CACAGATGTT 
     3061 ACCGTGAGAT ACGTACGGCT CCATCAGGCC GATGTGTCGT TAATAAATGC CAGTCTTCCG 
     3121 AAAGTGACAG GTGCAGCAAC AGCAGGGCAA GACTTCTTCA GAAGAGACAA CACATTAAAC 
     3181 CCCCAAAGCC ATTGCATGGT CTCAGGCAAA AGCACAGAGG GTTCATTTCT AAAGACAAAG 
     3241 GTTGCAAGGA TATGGGGGGC CATACAGAGG ATAGCGTTTC AGAACCTGAT GGGCAGATGA 
     3301 AATCTCCAGG AAATGACTTT CTATCTTCCA AAGATGACGC TCAACTCATG CACTTGATCC 
     3361 CCATCCCTGA ACTGTCCCCC GAACAGAAAG CACCCGCTCC AGTCTCAGAT CTATCCATTG 
     3421 TTGAGGAAAT CTTCACAGAG GAGTGTGCAG ACGAAGAAGG CATTCTCAAC GATGACAGCC 
     3481 TTACCCAAAG CCTTGGCGAT CTGAAAGAGC CTGAAGGGTT GCACCCCCAG GCCCCTCTCG 
     3541 TTCCTTCAGA AAACAGCTGG GCTGTGCTTA GCGAGAAGCG TTCGGGCAAG CGTGTGTCTC 
     3601 CAGAAAAGCA CAATGTAGAA CCTCTTGATA AAATAAATGC TGAGCAGATG TTCCCGGGTT 
     3661 ACCTGGAGTT TGATAGCCTT TCAGAGAAGT CTAAAACACT GGTGAGCTTT TCCAGTTGCT 
     3721 CTTTCCAGGA GAACCTGGAA AGAGCCCCTT CCCCTACAGA ACAACACTGG GCACGTTCTT 
     3781 TGGAGCAGGA CAGCTTAGAA AATAACTCCA CCACATACCA GACATTTGGA AAGATTTCCC 
     3841 AGGAAATCTT AGACCCAGGA AAGAATGAAG AGTTGACAGA CGAGTTGCTG GGTTGTCTAG 
     3901 TTGAAGAACT GTTAGCACTC GATGAGAAAG ATAACAACTC TTGCCAAATA ATGACAAATG 
     3961 AGGCAGACGC CAAAAACCTG AATCTTGTCT TCAGCAGGAG AGGCAATACC ATCGAAGAAC 
     4021 TGGGCAGAGA GACAACAGAT GTCAAACTTC AGAGGTGTAT TAATGGATTC CGGATATATG 
     4081 ATGAGGAAAA CTTTCTTACC TCAAATGAAA AGAAGACACT TTCTGACAAG AGCTTAAACC 
     4141 ACGAAGAGGC TATCTTCTGG ACCAAGGGTG AGATCCTTGG GAGAGGAGCC TATGGCACAG 
     4201 TATACTGTGG GCTCACCAGC CTAGGGCAGC TAATAGCTGT AAAACAGGTG GCCTTGGATA 
     4261 CTTCTGATAA ACTGGCTACC GAGAAGGAAT ACCGGAAACT GCAGGAGGAA GTGGATTTGC 
     4321 TGAAGGCACT GAAGCATGTC AACATAGTAG CTTATCTAGG GACGTGCTTG GAGGAGAACA 
     4381 CTCTGAGCAT CTTCATGGAA TTTGTCCCTG GTGGTTCCAT CTCTAGTATA ATAAACCGCT 
     4441 TTGGGCCACT GCCTGAGATG GTGTTCTGTA AATACACAAG ACAGATCCTG CAAGGTGTTG 
     4501 CCTATCTCCA TGACAACTGT GTGGTACATC GAGATATCAA AGGGAACAAC GTCATGCTCA 
     4561 TGCCAACGGG AATAATAAAG TTGATTGACT TCGGCTGTGC CAAGCGTTTG GCCTGGGCAG 
     4621 GCTTAAATGG TACCCACAGT GACATGCTCA AGTCCATGCG TGGGACTCCA TACTGGATGG 
     4681 CCCCGGAGGT CATCAATGAG TCTGGCTATG GAAGGAAATC AGATATATGG AGCATTGGCT 
     4741 GCACCGTGTT CGAGATGGCC ACAGGGAAGC CTCCTTTGGC TTCCATGGAT AGGATGGCAG 
     4801 CCATGTTCTA CATTGGAGCC CACAGAGGCC TAATGCCTCC TCTACCTGCC CGATTCTCAG 
     4861 AACCTGCTGC GGACTTCGTG CGTTTGTGCC TGACCAGGGA CCAGCATGAG AGACCTTCTG 
     4921 CTCTCCAGCT CTTGAAGCAC TCCTTCCTGA AGAGAAGCCA GACGCGTACG CGGCCGCTCG 
     4981 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5041 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5101 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5161 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5221 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5281 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5341 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5401 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5461 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5521 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5581 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5641 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5701 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5761 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5821 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5881 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5941 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6001 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6061 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6121 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6181 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6241 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6301 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6361 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6421 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6481 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6541 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6601 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6661 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6721 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6781 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6841 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6901 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6961 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7021 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7081 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7141 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7201 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7261 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7321 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7381 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7441 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7501 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7561 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7621 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7681 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7741 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7801 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7861 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7921 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7981 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8041 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8221 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8281 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8341 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8401 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8461 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8521 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8581 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8641 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8701 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8761 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//