LOCUS       MR221294                9051 bp    DNA     circular  SYN 
DEFINITION  Polr3a (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a)
ACCESSION   NM_001081247
VERSION     NM_001081247.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5208..5299 
                     /label="Myc-DDK"  
     misc_feature    5329..5914 
                     /label="PolyA signal"  
     misc_feature    5386..5405 
                     /label="Restriction Enzyme"  
     misc_feature    6190..6862 
                     /label="ColE1"  
     misc_feature    7292..8086 
                     /label="Kan/Neo"  
     misc_feature    8121..8423 
                     /label="SV40 ori"  
     misc_feature    8509..8986 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5190..5196 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5190 
                     /label="Polr3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAAGGAG CAGTTCCGCG AGACGGATGT GGCTAAGAAA ATAAGCCATA 
     1081 TCTGTTTTGG AATGAAGTCC CCTGAGGAGA TGCGCCAGCA GGCACATATC CAGGTTGTCA 
     1141 GTAAGAACCT GTACAGCCAA GACAACAACC ACGCCCCCTT GTTGTATGGA GTGCTTGACC 
     1201 ATAGAATGGG TACGAGCGAA AAAGATCGTC CTTGTGAGAC CTGTGGGAAG AACTTGGCGG 
     1261 ACTGTCTCGG CCACTATGGC TACATTGATC TCGAGCTACC GTGCTTTCAT GTCGGGTACT 
     1321 TCAGGGCTGT CATTGGTATC TTACAGATGA TCTGCAAAAC CTGCTGCCAC ATTATGCTGT 
     1381 CCCAGGAAGA GAAGCAGCAG TTTCTAGATT TTCTGAAGAG GCCTGGCCTA ACCTACCTGC 
     1441 AGAAGCGAGG CCTGAAGAAG AAGATCTCAG ATAAATGCCG GAAGAAAAGC ACTTGTCACT 
     1501 ACTGTGGTGC CTTTAATGGT ACCGTAAAGA AATGTGGTTT ACTGAAGATA ATTCATGAAA 
     1561 AATACAAGAC AAACAAAAAA GTCGTGGATC CCATTGTATC AAACTTCCTG CAGTCCTTTG 
     1621 AGACAGCCAT TGAGCATAAC AAAGAGGTTG AACCCCTGCT GGGAAGGGCC CAGGAGAACT 
     1681 TGAATCCTTT AGTAGTTCTG AACTTGTTTA AGCGCATCCC AGCTGAAGAT GTCCCGTTAC 
     1741 TCCTGATGAA CCCAGAATCT GGAAAGCCCT CTGATTTGAT TCTCACCCGA CTTTTGGTGC 
     1801 CTCCCCTGTG TATCAGACCG TCCGTCGTGA GTGATCTGAA GTCTGGAACC AATGAAGATG 
     1861 ACCTGACAAT GAAACTGACA GAGATCATTT TCCTAAATGA TGTTATTAAA AAGCACCGGA 
     1921 TCTCCGGAGC CAAGACGCAG ATGATCATGG AGGACTGGGA TTTCCTGCAG CTGCAGTGTG 
     1981 CCCTCTACAT CAACAGTGAG CTCTCTGGCA TCCCTCTCAA CATGGCACCT AAGAAATGGA 
     2041 CGCGAGGCTT TGTGCAGCGC CTGAAGGGGA AACAGGGTCG ATTTAGAGGT AATCTGTCAG 
     2101 GGAAGAGGGT GGATTTCTCC GGCCGAACAG TCATCTCACC TGACCCCAAC CTCCGGATTG 
     2161 ATGAAGTAGC CGTGCCAGTT CATGTGGCCA AAATTCTAAC TTTTCCTGAG AAGGTGAACA 
     2221 AAGCAAACAT CAACTTTTTG AGGAAACTGG TTCGAAATGG CCCAGATGTT CACCCAGGAG 
     2281 CCAATTTTAT CCAACAAAGA CACATGCAGA TGAAGAGGTT CCTGAAGTAT GGAAATCGGG 
     2341 AAAAGATGGC TCAGGAGCTC AAGTTCGGCG ACATTGTAGA GAGACACCTG ATAGATGGAG 
     2401 ATGTGGTCTT GTTCAACCGG CAGCCCTCCC TGCACAAACT GAGCATCATG GCCCACCTGG 
     2461 CCAGGGTTAA GCCCCACCGG ACCTTCAGAT TCAATGAATG TGTCTGCACC CCCTACAATG 
     2521 CAGACTTTGA TGGGGATGAA ATGAACCTTC ATCTTCCCCA GACAGAGGAA GCCAAAGCAG 
     2581 AGGCCCTTGT TCTCATGGGG ACCAAAGCGA ACCTTGTAAC CCCACGAAAT GGGGAGCCAC 
     2641 TCATTGCTGC CATTCAGGAC TTCTTAACAG GTGCTTACCT CCTCACCCTC AAGGACACCT 
     2701 TCTTTGACCG AGCCAAGGCT TGCCAGATCA TCGCTTCAAT ACTGGTTGGC AAAGATGAAA 
     2761 AAATTAAAGT GCGCCTCCCA CCCCCTACAA TACTAAAGCC TGTCACCCTG TGGACCGGAA 
     2821 AGCAGATCTT CAGTGTCATC CTCAGACCTA GTGATGACAA TCCAGTGCGG GCCAACCTCC 
     2881 GAACCAAGGG CAAGCAGTAC TGTGGCAAGG GGGAAGACCT CTGCGTCAAC GACTCCTATG 
     2941 TCACCATCCA GAACAGTGAG TTGATGTGCG GCAGCATGGA CAAAGGGACC CTGGGGTCGG 
     3001 GGTCCAAGAA CAACATCTTC TACATCTTGC TACGAGACTG GGGGCAGAAT GAGGCTGCCG 
     3061 ATGCCATGTC TCGGCTTGCC AGGCTGGCTC CTGTCTACCT GTCTAACCGT GGGTTCTCCA 
     3121 TCGGGATTGG TGATGTCACG CCTGGCCAGG GACTGCTGAA AGCCAAGTAT GAGCTACTAA 
     3181 ATGCTGGCTA CAAAAAATGT GACGAGTACA TTGAAGCCCT GAACACAGGC AAGCTGCAGC 
     3241 AGCAGCCAGG CTGCACGGCC GAGGAGACGC TTGAGGCTCT GATCCTGAAG GAGCTGTCTG 
     3301 TGATCCGGGA CCATGCTGGC AGTGCCTGCC TCCGCGAGCT GGACAAGAGC AACAGCCCCC 
     3361 TCACCATGGC CCTGTGTGGC TCCAAAGGTT CCTTCATAAA CATATCACAG ATGATTGCCT 
     3421 GTGTGGGACA GCAAGCCATC AGTGGCTCCC GGGTTCCAGA TGGCTTTGAG AACAGATCTT 
     3481 TGCCTCACTT TGAGAAGCAT TCAAAGCTCC CTGCTGCCAA AGGCTTTGTG GCCAATAGCT 
     3541 TTTACTCTGG CTTAACGCCT ACTGAGTTTT TCTTCCACAC AATGGCCGGT CGGGAGGGTC 
     3601 TGGTGGACAC AGCTGTAAAG ACTGCTGAAA CAGGATACAT GCAGAGAAGA CTGGTCAAAT 
     3661 CCCTGGAAGA CCTCTGCTCG CAGTACGATC TGACAGTGCG GAGCTCCACC GGTGACATCA 
     3721 TCCAGTTCAT CTATGGAGGA GATGGCTTAG ATCCTGCTGC CATGGAGGGC AAAGACGAAC 
     3781 CGCTGGAGTT TAAGCGGGTT CTGGACAACA TCAAGGCAGT GTATCCATGT CAGACTGAAC 
     3841 CTGCTCTCAG TAAGAACGAC CTCACCTTGA CCGCTGAGGC CATCATGAAG AAGAATGAGT 
     3901 TCCTATGCTG CCAAGACAGC TTCCTGCAGG AAATAAAAAC ATTCATTAAA GGGGTGTCCG 
     3961 AGAAGATTAA GAAAACCAGA GATAAATATG GGATCAATGA TAACGGCACA ACAGAGCCTC 
     4021 GTGTGCTGTA CCAGTTGGAC CGTATTACCC CTACCCAAAT AGAGAAGTTT CTGGAGACCT 
     4081 GTAGGGATAA GTATATGAGA GCACAGATGG AGCCAGGCTC TGCGGTAGGT GCACTCTGTG 
     4141 CACAGAGCAT TGGTGAGCCC GGCACCCAGA TGACGCTGAA GACATTCCAC TTTGCAGGAG 
     4201 TAGCCTCCAT GAACATCACC CTGGGTGTGC CTCGGATCAA AGAGATCATC AACGCTTCCA 
     4261 AGGCCATCAG CACTCCAATT ATCACAGCAC AGCTAGACAA GGATGACGAC GCGGATTATG 
     4321 CACGCCTGGT GAAGGGCAGG ATTGAGAAAA CCCTCCTGGG GGAGATCTCA GAGTATATTG 
     4381 AAGAAGTGTT TCTTCCTGAT GACTGCTTTA TTCTTGTCAA GCTCTCCCTG GAACGGATCC 
     4441 GACTTCTGAG ACTGGAGGTC AATGCTGAGA CAGTGCGGTA CTCCATCTGC ACATCCAAGC 
     4501 TCCGAGTGAA GCCTGGTGAT GTGGCCGTTC ATGGGGAGGC TGTGGTGTGT GTCACCCCCA 
     4561 GGGAAAACAG CAAGAGCTCC ATGTATTATG TGCTGCAGTT TCTCAAAGAG GATCTGCCCA 
     4621 AGGTGGTTGT ACAAGGCATC CCAGAGGTGT CGCGAGCTGT CATCCATATT GACGAGCAGA 
     4681 GTGGGAAGGA GAAGTTCAAG CTTCTGGTGG AAGGTGACAA CCTGCGGGCA GTCATGGCCA 
     4741 CCCACGGTGT GAAAGGCACC CGGACCACGT CTAATAACAC CTATGAGGTG GAAAAAACCC 
     4801 TGGGCATTGA GGCTGCCCGG ACAACTATCA TCAACGAGAT CCAGTACACG ATGGTCAACC 
     4861 ACGGCATGAG CATCGACAGG AGGCACGTGA TGCTGCTCTC TGACCTGATG ACCTACAAGG 
     4921 GTGAAGTCCT GGGTATAACC AGGTTTGGCC TGGCCAAGAT GAAGGAGAGT GTGTTGATGC 
     4981 TGGCCTCCTT TGAAAAGACA GCTGACCATC TCTTTGATGC TGCCTACTTT GGGCAGAAAG 
     5041 ACTCTGTATG CGGAGTGTCT GAGTGCATCA TCATGGGAAT CCCGATGAAC ATCGGAACTG 
     5101 GGCTCTTCAA GCTGTTACAC AAAGCCAACA GGGACCCCAA ACCACCCAGG AGACCCCTGA 
     5161 TATTTGACAC ACATGAATTC CACATCCCTC TTGTTACAAC GCGTACGCGG CCGCTCGAGC 
     5221 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5281 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5341 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5401 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5461 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5521 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5581 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5641 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5701 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5761 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5821 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5881 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5941 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6001 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6061 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6121 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6181 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6241 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6301 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6361 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6421 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6481 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6541 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6601 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6661 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6721 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6781 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6841 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6901 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6961 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7021 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7081 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7141 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7201 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7261 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7321 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7381 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7441 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7501 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7561 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7621 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7681 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7741 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7801 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7861 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7921 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7981 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8041 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8101 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8161 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8221 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8281 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8341 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8401 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8461 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8521 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8581 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8641 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8701 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8761 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8821 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8881 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8941 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9001 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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