LOCUS       MR221273                7707 bp    DNA     circular  SYN 
DEFINITION  Itpkb (Myc-DDK-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase B (Itpkb)
ACCESSION   NM_001081175
VERSION     NM_001081175.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3864..3955 
                     /label="Myc-DDK"  
     misc_feature    3985..4570 
                     /label="PolyA signal"  
     misc_feature    4042..4061 
                     /label="Restriction Enzyme"  
     misc_feature    4846..5518 
                     /label="ColE1"  
     misc_feature    5948..6742 
                     /label="Kan/Neo"  
     misc_feature    6777..7079 
                     /label="SV40 ori"  
     misc_feature    7165..7642 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3846..3852 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3846 
                     /label="Itpkb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGTAC TGCTATGCCC TCAATAGCCT GGTGATCATG AACAGCACCA 
     1081 ACGAGCTCAA GAGTGGCGGC CCCCGGCCCA GCGGCAGCGA GACGCCCCAG CCCTCCGGGA 
     1141 GGGCCGCGCT GAGTCCCGGC AGCGTCTTCA GCCCTGGGAG AGGCGCTTCC TTTCTCTTCC 
     1201 CCCCAGCAGA GTCGCTGTCC CTGGAGGAGC CCGGGAGTCC TGGGGGCTGG CGCAGCGGCC 
     1261 GGCGCAGGCT GAATAGTAGC AGCGGTAGCG GAGGTGGCAG CAGCAGCAGC AACAGCAGCA 
     1321 GCAGCAGTGG CGTGGGCAGT CCCAGTTGGG CTGGCCGCCT GCGAGGGGAC GCGCAGCAGG 
     1381 TGGTGGCGGC CCGCATCCTC TCCCCACCTG GGCCAGAGGA GGCCCAGAGG AAGCTGCGGA 
     1441 TTCTGCAGCG CGAGTTGCAA AATGTGCAGG TGAACCAGAA AGTGGGCATG TTCGAGGCGC 
     1501 AAATCCAGGC ACAGAGCTCT GCCATTCAAG CGCCCCGAAG CCCGCGTTTG GGTAGGGCTC 
     1561 GTTCGCCCTC CCCGTGTCCC TTCCGAAGTA GCAGCCAGCC CCCCGAAAGG GTCTTGGCTC 
     1621 CGTGTTCTCC AAGTGAGGAA CGCAGAACAA AGTCCTGGGG AGAACAATGT ACAGAAACCC 
     1681 CAGATACCAA CTCTGGAAGG AGAAGCAGAC TCAGCACACA CCCCTCGAAG GACAAGGAGG 
     1741 GAGTGGCCCC TCTTTTAGGC CCAGCCAGCC CGACCAGGTT AGGGACTCAG AGTCCATCTA 
     1801 CTTCCGTGAG AATGGAAAGA GGTACCCCGG CCAGTCCCCG CTGTGGCTCA CCCACACCCA 
     1861 TGGAAACTGA CAAGAGGGTT GCTCCCTCAC TGGAGCGCTT TGGAACTAGC TTAACGTTGG 
     1921 CTACTAAAGT GGCAGCTTCA GCCGCATCCG CTGGACCACA CCCTGGACAT GATTCTGCCC 
     1981 TCATGGAGAC AGGCTGTGAG CTCGGGGGCA TGCGCCCCTG GGAGGCCCAG ATGGAGAGAC 
     2041 GGGGGCAGTT TCTGGGCAAG GAGACCGGCT CAACCCCAGA GCCTGTCCGG ACCCACATGA 
     2101 GAGAACCCCC TGGAAGGGTG GGAAGAGGAA TTCATTCTGT TGGTGGGCAG GGCTCCTGGA 
     2161 CACCTGAAGT CATCAAAAGA CCAGAAGAGA GGGCTGTGAC TGCCCAAAGC TCAGAGCCCT 
     2221 CAGAGGACCC CAGATGGTCT AGACTGCCTG TAGACCTGGA TTCCGTAGGA CCTGAGAAGG 
     2281 GAGGAAATAG GATCCCAGGG ATGCGAGGGC CCCAGCAGAC CCTGGACAGT GAGAGAGAAG 
     2341 GCTCTCCAGC ACTGGGCTTG CTTGGGGGCA GCCAGGCAGC ACAGCCAGGG GCTAGGGGTG 
     2401 TGGAGGAAGA CGTTCATTAT GGCCGAATGC TGGAACCTTT ACCACCTGGG GAAGTAACAA 
     2461 CAAAATTGAA AGAACCCCAG TGCCTCCCTG GGGACAGGAT GGGAATGCAG CCTGAGAGTT 
     2521 CCATAGTTTG GCCCAGTGCC TTGGAGGAAG CTCCCCTGAT CTGGACACGT GACACGGGGG 
     2581 TACAGTCGAA GGGGACTTGG GGAAGCCAGC TGCCAGACGG AGATGCTCAC CCTAGCTGCC 
     2641 AAGAGATGCC CCCAGACCAG AAGGACAAGG CCTCCTTAAA AGAGGCCTGC AGCCCCAGCA 
     2701 ACATCCCAGC CATCCCTGCA GTCATCATTA CAGATATGGG TGCTCAGGAG GATGGAGGGC 
     2761 TAGAGGAGAT CCAAGGAAGC CCTCGGGGTC CCCTGCCTCT GAGGAAGCTG TCCTCCTCCT 
     2821 CAGCCTCCTC CACTGGCTTC TCCTCTTCTT ACGATGACTC GGAGGAGGAC ATCTCCAGTG 
     2881 ACCCTGAGCG CACGCTGGAC CCCAACTCAG CCTTTTTGCA TACCCTGGAC CAGCAGAAGC 
     2941 CCAGAGTGAG CAAGTCATGG AGAAAGATAA AGAACATGGT GCAGTGGTCC CCCTTTGTCA 
     3001 TGTCCTTCAA GAAGAAGTAC CCCTGGATCC AGCTGGCAGG ACATGCAGGG AGCTTCAAGG 
     3061 CAGCTGCAAA TGGCCGCATC CTTAAGAAGC ACTGTGAGTC TGAGCAGCGC TGCCTGGACC 
     3121 GGTTAATGGC GGATGTGCTG AGACCCTTCG TGCCAGCCTA CCATGGGGAC GTGGTGAAGG 
     3181 ATGGGGAACG CTACAACCAG ATGGACGACC TGCTGGCTGA CTTCGATTCG CCCTGCGTGA 
     3241 TGGACTGCAA GATGGGTGTC AGGACATACC TGGAGGAAGA ACTCACCAAG GCCCGGAAGA 
     3301 AGCCTAGCTT GCGGAAGGAC ATGTACCAGA AGATGGTCGA GGTGGACCCT GAGGCCCCCA 
     3361 CTGAAGAGGA GAAAGCCCAG CGAGCTGTGA CCAAGCCACG TTACATGCAG TGGCGGGAAA 
     3421 CCATCAGTTC GACAGCCACC CTAGGCTTCA GGATCGAAGG CATCAAGAAG GAAGATGGCT 
     3481 CTGTGAACCG TGACTTCAAG AAGACCAAAA CAAGGGAGCA GGTCACCGAG GCTTTCAGAG 
     3541 AATTCACTAA GGGAAACCAG AACATCTTGA TCGCCTACCG GGACCGGCTG AAGGCCATTC 
     3601 GAGCGACCCT GGAAATCTCT CCCTTCTTCA AGTGCCACGA GGTCATTGGC AGCTCTCTCC 
     3661 TCTTCATCCA TGACAAGAAG GAGCAAGCCA AGGTGTGGAT GATTGACTTT GGGAAAACCA 
     3721 CGCCCCTTCC GGAAGGCCAG ACCCTACAAC ACGACGTCCC CTGGCAGGAG GGGAACCGGG 
     3781 AGGATGGCTA CCTCTCAGGG CTGGACAACC TCATCGACAT CCTGACGGAA ATGTCCCAGG 
     3841 GCAGCCCACT CACCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3901 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3961 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4021 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4081 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4141 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4201 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4261 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4321 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4381 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4441 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4501 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4561 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4621 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4681 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4741 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4801 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4861 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4921 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4981 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5041 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5101 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5161 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5221 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5281 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5341 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5401 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5461 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5521 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5581 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5641 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5701 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5761 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5821 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5881 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5941 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6001 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6061 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6121 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6181 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6241 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6301 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6361 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6421 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6481 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6541 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6601 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6661 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6721 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6781 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6841 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6901 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6961 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7021 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7081 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7141 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7201 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7261 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7321 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7381 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7441 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7501 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7561 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7621 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7681 ATTTAACAAA AATTTAACGC GAATTTT
//