LOCUS       MR221236                7827 bp    DNA     circular  SYN 
DEFINITION  Pcdha9 (Myc-DDK-tagged) - Mouse protocadherin alpha 9 (Pcdha9). Note: ORF is codon optimized
ACCESSION   NM_138661
VERSION     NM_138661.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3982..4073 
                     /label="Myc-DDK"  
     misc_feature    4103..4688 
                     /label="PolyA signal"  
     misc_feature    4160..4179 
                     /label="Restriction Enzyme"  
     misc_feature    4964..5636 
                     /label="ColE1"  
     misc_feature    6066..6860 
                     /label="Kan/Neo"  
     misc_feature    6895..7197 
                     /label="SV40 ori"  
     misc_feature    7283..7760 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3957..3964 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3957 
                     /label="Pcdha9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCTGGGG AACAGACCGG AGGATATCCG GACGTGTGTG CACCTGAGAT 
     1081 GGCACATCCA CGGCCTCCTT AGGCAGGAGA ATGCTTCCGT CGTGATCTCA AAATGTCTGC 
     1141 GCCACGGAGC CTGGCGCCTC CTTCTGTGGC TTCTCCTGCT CGCCACTTGG GACGTGGGAA 
     1201 GTGGCCAACT GCACTACTCC GTGCCTGAAG AGGCGAAGCA TGGTACCTTT GTCGGACGAA 
     1261 TTGCACAGGA TCTTGGCCTG GAGCTTGCCG AACTTGTCCC CAGACTGTTC CGGGTGGTGT 
     1321 CCAAGGATAG GGGAGATTTG TTGGAGGTCA ACCTGCAAAA CGGGATCCTT TTCGTAAACA 
     1381 GCAGGATCGA TCGAGAGGAG CTGTGCGGTC AGAACGCCGA ATGCAGCATC CACCTGGAGG 
     1441 TGATCGTCGA CCGCCCTCTG CAAGTGTTTC ACGTAGAGGT CGAGGTGCGC GACATCAACG 
     1501 ACAACCCGCC TATTTTCAGC GTGGCTGAGC AGAAAATTCT GGTCGCAGAG AGCCGGCTTC 
     1561 TGGACTCCCG ATTCCCCCTG GAAGGAGCCT CCGACGCTGA TGTCGGCGAA AACTCCATGC 
     1621 TCACCTACAA ACTGAGCAGT AATGAATTTT TCATCCTCGA TATCGTGAAT AAGAGAGGTA 
     1681 AAGGGAAGTT TCCAGTCCTG GTGCTGAGAA AGTTGATAGA TCGGGAGGAA AATCCCCAGC 
     1741 TGAAGTTGCT GCTGACCGCA ACGGACGGAG GCAAACCCGA ATTCACCGGA TCCGTCAGCC 
     1801 TGCTCATCCA GGTGTTGGAC GTGAACGATA ATGCACCGGT GTTCGATAGA TCTGTCTATG 
     1861 AGGTGAAGAT GTACGAGAAT CAGGAGAATA AAACTTTGGT GATCTGGCTG AACGCGACCG 
     1921 ACTCTGACGA GGGAATAAAC AAAGAAGTGG AGTACAGTTT TTCCAGCTTG GCAAGTAGTA 
     1981 TTATCCGACA GAAGTTCCTG ATCAACGAAA AGACAGGAGA GATAAAAATT AATGGCGCTA 
     2041 TTGATTTTGA GGAGAGTAAT AACTATGAGA TACACGTCGA TGCCACGGAT AAGGGATATC 
     2101 CTCCTATGGT GGCCCACTGC ACTGTACTCG TTGAGATCCT GGACGAAAAT GACAATGCCC 
     2161 CTGAGATTGT GTTGACGAGC CTGTCCCTTC CCGTAAAAGA GGACGCGCCG CTTGGCTCCG 
     2221 TGATTGCACT TATCAGTGTA TCTGATAAGG ACTCTGGCGT TAACGGTCAG GTGACATGCA 
     2281 GCCTGACCAA TCACGTACCT TTTAAGCTGG TGAGCACTTT CAAGAATTAT TATTCTCTTG 
     2341 TGCTGGACAG CGCTCTCGAC AGGGAGACAA CAGCCGATTA TAAGGTTGTC GTCACTGCAA 
     2401 GGGACGGCGG GTCACCAAGT CTGTGGGCCA CCGCCAGTGT GAGTGTCGAA GTGGCCGACG 
     2461 TGAATGACAA CGCTCCCGCC TTCGCTCATC CTGAGTATAC TGTGTTCGTG AAAGAGAACA 
     2521 ATCCCCCTGG CGTGCACATT TTTACTGTGT TGGCCGTGGA CGCGGACGCT CAGGAAAACG 
     2581 CTCTGGTGAG TTACAGCCTG GTGGAAAGGC GCGTTGGAGA AAGGCTGCTC AGCTCTTACG 
     2641 TGTCCGTGCA CGCCGAAAGC GGAAAGGTGT TTGCACTGCA GCCTTTGGAT CATGAGGAGC 
     2701 TGGAGTTGCT GCAGTTCCAG GTTTCCGCCA GAGACGCCGG TGTCCCAGCC CTGGGCTCCA 
     2761 ACGTCACCCT GCAGGTGTTC GTGCAGGATG AGAACGACAA TCCCCCCACT CTCCTGGGAC 
     2821 ACCAGTCAGG CGGCCCAGCT GGGGAGTTCT CTCAGCTTGT GAGTAGATCT GTAGGGGCCG 
     2881 GTCATGTAGT GTCAAAGGTG CGAGCCGTCG ACGCCGACAG CGGTTATAAC GCATGGTTGA 
     2941 GTTACGAACT CCACCCTACG GTGGGTGCAC GATCTCCATT CCGGGTCGGT CTGTACACCG 
     3001 GTGAGATTTC TATGACTAGG GCTCTGGACG AGAGCGATCT GCCTCGGCAG CGGTTGCTGG 
     3061 TCTTGGTCAA GGATCACGGG GAACCTATGC TCATCGCCAC AGCTACTGTA CTCGTATCCT 
     3121 TGGTTGAGAA CGGCCAGGTA CCTAAGGCTA GTTCTCAGGG GCTCCCAAAC AGCTCTAGGA 
     3181 GAGAGGCCAG CCTGATGGAT GTGAATGTTT ATCTCATTAT TGCCATCTGC GCTGTGTCAT 
     3241 CTCTGCTCGT CCTGACTCTT CTCCTTTACA CTGCGCTTAG ATGTTCAGCC GTGCCTATGC 
     3301 AAGCAGGATG TGGCCTCGGT AAGCCAACCC TCGTCTGTTC TAGCGCCGTG GGGACATGGT 
     3361 CTTACTCACA ACAGCGCCAG CAGAGAGTCT GTAGTGGCGA GGGACCTCCA AAGACCGACC 
     3421 TTATGGCTTT TAGTCCATCA TTGACCCCCT GCCCTGTGGC TGAGGTAGGT ATGGAGTCAC 
     3481 ACTCTGTCGG TGGCGACGTT CCCGGCAAGC CAAGGCAGCC CAACCCAGAT TGGCGCTACT 
     3541 CTGCTTCCCT TCGGGCCGGC ATGCATTCCT CCGTCCACCT GGAAGAGGCA GGTATTCTCC 
     3601 GAGCCGGTCC AGGAGGCCCT GATCAACAAT GGCCCACCGT TTCTTCAGCT ACTCCTGAGC 
     3661 CTGAGGCCGG GGAGGTGTCC CCCCCTGTCG GGGCTGGAGT GAACAGCAAC TCATGGACAT 
     3721 TTAAGTATGG TCCCGGGAAC CCAAAGCAGA GCGGTCCCGG AGAGCTGCCA GATAAGTTTA 
     3781 TTATTCCAGG CAGTCCCGCA ATCATCAGTA TTAGACAAGA ACCAGCCAAT AACCAGATCG 
     3841 ACAAGTCCGA TTTCATTACC TTCGGTAAGA AAGAAGAAAC CAAGAAAAAG AAGAAAAAAA 
     3901 AAAAAGGCAA CAAAACCCAG GAGAAAAAAG AGAAGGGTAA CAGTACAACC GATAATAGCG 
     3961 ACCAGAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4021 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4081 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4141 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4201 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4261 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4321 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4381 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4441 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4501 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4561 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4621 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4681 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4741 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4801 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4861 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4921 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4981 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5041 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5101 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5161 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5221 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5281 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5341 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5401 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5461 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5521 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5581 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5641 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5701 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5761 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5821 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5881 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5941 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6001 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6061 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6121 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6181 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6241 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6301 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6361 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6421 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6481 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6541 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6601 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6661 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6721 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6781 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6841 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6901 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6961 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7021 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7081 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7141 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7201 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7261 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7321 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7381 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7441 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7501 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7561 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7621 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7681 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7741 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7801 ATTTAACAAA AATTTAACGC GAATTTT
//