LOCUS       MR221223                8286 bp    DNA     circular  SYN 
DEFINITION  Kcnt2 (Myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 2 (Kcnt2)
ACCESSION   NM_001081027
VERSION     NM_001081027.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="Kcnt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTGATTTG GAGAGCGAAG TGCCCCCTCT GCCTCCCAGG TACAGATTCC 
     1081 GGGATTTGCT GCTAGGGGAC CAAGGCTGGC AAAATGATGA CAGGGTCCAA GTTGAATTCT 
     1141 ACATGAACGA AAACACATTT AAAGAGAGAT TAAAACTGTT TTTCATAAAA AACCAGCGAT 
     1201 CAAGTCTAAG AATCCGCTTG TTCAACTTTT CCCTGAAGTT GTTAAGCTGC TTATTATATA 
     1261 TTATCCGTGT GTTGCTTGAG AAACCGTCAC AGGGAAGTGA ATGGTCTCAC ATATTTTGGG 
     1321 TTAACCGAAG TCTACCTTTA TGGGGTTTAC AGGTCTCAGT GGCATTGATA AGTCTATTTG 
     1381 AAACAATACT CCTTGGTTAT CTCAGTTATA AGGGAAATAT CTGGGAGCAG ATTTTACGAA 
     1441 TTCCATTCAT CTTGGAAATA ATCAATGCAG TCCCCTTCAT CATCTCAATA TTCTGGCCCA 
     1501 CCTTAAGAAA TCTATTTGTT CCAGTTTTTC TAAATTGTTG GCTTGCCAAA CATGCCTTGG 
     1561 AAAATATGAT TAATGATCTC CATAGAGCCA TTCAGCGTAC ACAGTCTGCA ATGTTTAATC 
     1621 AAGTTTTGAT TTTGATATCT ACTTTACTAT GCCTTATCTT CACTTGCATT TGTGGGATTC 
     1681 AGCATCTAGA ACGAATTGGC AAGAAGCTCA ATCTCTTTGA CTCCCTTTAT TTCTGCATTG 
     1741 TGACATTTTC TACTGTGGGC TTTGGGGATG TCACTCCTGA AACATGGTCC TCCAAGCTCT 
     1801 TTGTCGTTGC TATGATCTGC GTTGCCCTTG TAGTACTCCC TATACAGTTT GAACAGTTGG 
     1861 CCTATTTGTG GATGGAGAGA CAAAAGTCAG GAGGAAACTA CAGTCGACAC AGAGCTCAGA 
     1921 CAGAGAAGCA TGTTGTCCTG TGTGTCAGCT CCTTGAAGAT TGACTTACTT ATGGATTTTC 
     1981 TAAATGAGTT CTATGCGCAT CCAAGACTAC AGGATTATTA CGTGGTGATT TTGTGTCCCA 
     2041 CGGAGATGGA TGTGCAAGTT CGGAGAGTAC TACAGATTCC AATGTGGTCC CAGAGAGTGA 
     2101 TCTACCTTCA AGGCTCTGCC CTTAAAGACC AGGATCTCCT GAGAGCAAAG ATGGACAATG 
     2161 CTGAGGCCTG TTTCATTTTG AGTAGCCGCT GCGAAGTGGA TAGAACCTCA TCTGATCACC 
     2221 AAACAATCTT GAGAGCATGG GCTGTGAAAG ATTTTGCCCC AAATTGTCCT CTGTATGTTC 
     2281 AGATATTAAA ACCAGAAAAC AAATTCCACA TCAAATTTGC AGATCATGTT GTTTGTGAAG 
     2341 AAGAGTTTAA GTACGCCATG TTAGCTTTAA ACTGTATATG CCCAGCAACA TCTACACTCA 
     2401 TTACACTACT GGTTCATACC TCTAGAGGGC AAGAAGGGCA GCAGTCACCT GAACAGTGGC 
     2461 AGAAGACTTA CGGGAGGTGC TCAGGAAACG AAGTCTATCA CATCGTTTTG GAAGAAAGTA 
     2521 CATTTTTTGC TGAATATGAA GGGAAGAGTT TTACCTATGC TTCTTTTCAT GCCCACAAAA 
     2581 AGTTTGGTGT CTGCTTGGTT GGTGTTAGGA GGGAGGATAA TAAAAACATT TTGCTGAATC 
     2641 CAGGTCCTCG ATACATTATG AATGCTTCAG ACATATGTTT TTATATTAAT ATTACCAAAG 
     2701 AAGAAAATTC AGCATTTAAG AATCAAGACC AACAGAGAAA AAGTAATGTG TCAAGGTCAT 
     2761 TTTATCATGG GCCTTCCAGA TTGCCTGTCC ACAGCATCAT TGCCAGCATG GGTACTGTGG 
     2821 CTATAGACTT GCAAGACACA AGCTGCAGAG CAACAAGTGG CCCCACCCTG GCTCTTCCTT 
     2881 CAGAGGGAGG CAAAGAATTA AGAAGACCTA GCATTGCTCC TGTTTTAGAG GTGGCAGATA 
     2941 CATCATCAAT TCAAACATGC GATCTCCTAA GTGACCAGTC CGAAGATGAA ACTACACCAG 
     3001 ATGAAGAAAC ATCTTCAAAT TTAGAGTATG CCAAAGGCTA CCCACCTTAT TCCCCATACA 
     3061 TAGGAAGTTC ACCTACTTTT TGTCACCTAC TTCAAGAAAA AGTGCCATTT TGCTGCTTAA 
     3121 GATTAGACAA GAGTTGCCAG CATAACTACT ATGAGGACGC AAAAGCCTAT GGATTCAAAA 
     3181 ATAAACTAAT TATAGTTGCA GCTGAAACAG CTGGAAATGG ACTATATAAT TTTATTGTAC 
     3241 CTCTCAGGGC ATATTATAGA CCAAAGAAAG AATTAAATCC CATAGTTCTG CTATTGGATA 
     3301 ACCCGCCAGA TATGCATTTT CTGGATGCAA TCTGTTGGTT TCCAATGGTT TACTACATGG 
     3361 TGGGCTCTAT TGACAACCTA GATGATTTGC TCAGGTGTGG AGTGACCTTT GCTGCCAACA 
     3421 TGGTGGTGGT GGACAAAGAA AGCACCATGA GTGCAGAGGA AGACTACATG GCAGATGCCA 
     3481 AGACGATTGT GAATGTGCAG ACTCTGTTCA GGTTGTTTTC AAGTCTCAGT ATCATCACTG 
     3541 AGCTTACGCA TCCAGCAAAT ATGAGATTCA TGCAATTCAG AGCCAAGGAT TGTTACTCTC 
     3601 TCGCGCTTTC AAAACTGGAA AAGAAAGAAA GAGAACGAGG TTCTAACTTG GCCTTTATGT 
     3661 TTCGACTGCC TTTTGCTGCG GGAAGAGTGT TCAGCATCAG TATGTTAGAC ACCCTTCTTT 
     3721 ATCAGTCATT TGTGAAAGAT TATATGATTT CTATCACCAG ACTTCTTTTG GGACTGGACA 
     3781 CCATACCAGG ATCGGGGTTT CTTTGTTCTA TGAAAATCAC TGAAGATGAC TTGTGGATCA 
     3841 GAACGTATGC CAGACTCTAT CAGAAGTTGT GTTCTTCTAC TGGAGATGTT CCCATCGGGA 
     3901 TCTACAGAAC AGAATCTCAG AAACTAACGA CATCTGAGTC TCAAATATCC ATTAGTGTGG 
     3961 AAGAGTGGGA AGACACCAAA GATGTCAAAG ACCCAGGGCA CCACCGCAGC CTTCACCGCA 
     4021 ACTCAACGTC TAGTGACCAG TCGGACCATC CCTTGCTGCG GAGGAAGAGC ATGCAGTGGG 
     4081 CCCGAAGGCT GAGCAGAAAA GGGCCGAAGC ACTCTGGTAA AACTGCAGAA AAAATAACTC 
     4141 AACAGCGACT GAACCTCTAC CGGAGATCCG AAAGACAGGA GCTTGCTGAA CTTGTGAAAA 
     4201 ACAGAATGAA ACACCTGGGC CTCTCGACGG TGGGCTACGA TGAAATGAAT GATCATCAGA 
     4261 GCACCCTGTC CTACATCCTG ATTAATCCGT CTCCAGACAC CAGGCTTGAG CTGAATGATG 
     4321 TTGTATATTT GATCCGACCA GATCCACTGT CCTACCTTCC AAACAGTGAA CCCAGTAGGA 
     4381 AAAACAGCAT CTGCAATGCC GCTGTTCAAG ATTCTCGGGA GGAAACTCAA CTCACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//