LOCUS       MR221139                8385 bp    DNA     circular  SYN 
DEFINITION  Spag9 (Myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 3
ACCESSION   NM_001025429
VERSION     NM_001025429.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4542..4633 
                     /label="Myc-DDK"  
     misc_feature    4663..5248 
                     /label="PolyA signal"  
     misc_feature    4720..4739 
                     /label="Restriction Enzyme"  
     misc_feature    5524..6196 
                     /label="ColE1"  
     misc_feature    6626..7420 
                     /label="Kan/Neo"  
     misc_feature    7455..7757 
                     /label="SV40 ori"  
     misc_feature    7843..8320 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4524..4530 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4524 
                     /label="Spag9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCCTGGA TGCATGCTGC TGTTTGTGTT TGGCTTTGTT GGCGGGGCGG 
     1081 TGGTCATTAA TTCTGCGATC TTAGTATCTC TCTCTGTTTT GCTGCTTGTG CACTTTTCTA 
     1141 TTTCTACCGG GGTGCCAGCG CTGACGCAGA ACCTACCAAG GATACTCAGA AAAGAACGTC 
     1201 CTATATCATT AGGGATTTTC CCTCTACCTG CTGGAGATGG ATTGCTTACA CCTGACACTC 
     1261 AGAAAGGAGG CGAGACCCCA GGATCAGAGC AATGGAAATT TCAAGAATTA AGTCAACCAC 
     1321 GTTCTCATAC CAGCCTGAAG GATGAACTTT CTGATATTAG TCAAGGTGGA TCTAAAGCTA 
     1381 CCACTCCAGC TTCAACAGCA AATTCAGACG TATCAGCAAT TCCTCCTGAT ACTCCGTCAA 
     1441 AGGAAGATAA TGAAGGATTT GTAAAAGGCA CAGATACATC AAATAAGTCA GAGATAAGCA 
     1501 AACACATAGA AGTCCAGGTT GCCCAAGAGA CTAGGAATGT GTCTACAGAA TCTGGTGAAA 
     1561 ATGAAGAGAA ATCAGAAGTT CAAGCAATCA TTGAATCCAC TCCTGAATTG GATATGGACA 
     1621 AAGACCTCAG TGGATATAAA GGTTCAAGCA CTCCCACCAA AGGCATAGAG AACAAAGCTT 
     1681 TTGATCGCAA TACAGAATCT CTCTTTGAAG AGCTGTCCTC AGCTGGCTCG GGCCTTATTG 
     1741 GAGATGTGGA TGAAGGAGCG GATTTACTAG GGGAATATTC TGGAATGGGT CGTGAGGTGG 
     1801 AGAATCTCAT ACTGGAGAAC ACACAGCTGC TGGAAACCAA AAACGCTCTG AATGTAGTGA 
     1861 AGAATGACTT AATAGCCAAA GTGGATGAGC TGACCTGTGA GAAGGACGTG CTGCAGGGGG 
     1921 AGCTGGAGGC TGTGAAGCAA GCCAAGCTGA AGCTGGAGGA CAAGAACAGA GAGCTGGAGG 
     1981 AGGAGCTCAG GAAAGCTCGT GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGACGATG 
     2041 ATGATAGTGA CATTCCCACA GCCCAGAGGA AGAGGTTTAC CAGAGTAGAA ATGGCCCGAG 
     2101 TTCTCATGGA GAGAAACCAG TACAAAGAGA GGCTGATGGA GCTTCAAGAA GCTGTTCGAT 
     2161 GGACGGAGAT GATCCGGGCC TCACGAGAGA ACCCAGCTAT GCAAGAAAAG AAAAGGTCAA 
     2221 GCATCTGGCA ATTTTTCAGC CGACTTTTTA GCTCTTCAAG TAATGCAACA AAGAAGCCTG 
     2281 AACCCCCTGT TAACCTGAAG TACAACGCCC CCACCTCTCA CGTGACTCCC TCCGTGAAGA 
     2341 AAAGGAGCAG CACTTTATCT CAGCTCCCTG GGGACAAGTC CAAGGCATTT GATTTCCTCA 
     2401 GTGAGGAAAC TGAGGCTAGT TTAGCCTCCC GCAGAGAGCA AAAGAGAGAG CAGTATCGGC 
     2461 AGGTGAAGGC TCACGTTCAG AAGGAAGATG GCCGAGTGCA AGCTTTCGGC TGGAGTCTGC 
     2521 CTCAGAAGTA CAAACAGGTA GCCAATGGTC AAGGGGAAAC TAAGATGAAA AATTTACCTG 
     2581 TGCCTGTCTA TCTCAGACCA CTAGACGAAA AAGATGCGTC TATGAAGCTG TGGTGTGCTG 
     2641 TAGGAGTCAA TTTATCTGGT GGCAAGACAA GAGATGGCGG GTCTGTTGTT GGAGCGAGTG 
     2701 TATTTTACAA GGATATTGCT GGTTTGGACA CTGAAGGCAG TAAACAGCGG AGTGCGTCTC 
     2761 AGAGTAGTTT AGACAAGTTG GATCAAGAAC TCAAGGAACA GCAGAAAGAA TTTAAAAATC 
     2821 AAGAAGAGTT GTCCAGTCAG GTCTGGATCT GTACCAGCAC CCACTCAACT ACAAAGGTTA 
     2881 TCATCATTGA TGCTGTTCAG CCTGGCAACA TCCTAGATAG TTTCACTGTT TGCAATTCTC 
     2941 ATGTTCTGTG CATTGCCAGT GTCCCAGGAG CTCGGGAAAC AGACTACCCT GCAGGAGAAG 
     3001 AACTTTCTGA ATCTGGTCAG GTAGACAAAG CGTCATTGTG TGGAAGCATG ACCAGTAACA 
     3061 GCTCCGCAGA GATGGACAGC TTGCTGGGAG GCATCACAGT GGTTGGGTGC TCCACAGAAG 
     3121 GACTGACAGG AGCTGCCACT TCCCCAAGTA CCAACGGTGC TTCTCCTGTG ATTGAAAAGC 
     3181 CACCAGAAAT GGAAACTGAA AATAGCGAAG TTGATGAAAA TATTCCAACA GCAGAAGAAG 
     3241 CAACTGAAGC CACAGAGGGC AATGCAGGGT CCACTGAAGA CACTGTGGAC ATCTCCCAGC 
     3301 CTGGCGTGTA CACAGAGCAT GTGTTTACAG ATCCACTGGG AGTTCAGATC CCAGAAGACC 
     3361 TCTCCCCAGT GTTTCAGTCA AGTAATGACT CAGATGTGTA CAAAGATCAA ATATCAGTAT 
     3421 TGCCAAATGA ACAAGACCTT GCGAGAGAAG AAGCTCAGAA AATGAGTAGT CTTTTACCAA 
     3481 CCATGTGGCT TGGAGCTCAG AATGGCTGTT TGTATGTCCA CTCATCTGTA GCCCAGTGGA 
     3541 GGAAATGTCT CCATTCCATT AAGCTCAAAG ACTCAATTCT CAGTATTGTA CATGTGAAAG 
     3601 GAATTGTACT AGTGGCCCTG GCGGATGGCA CCCTTGCAAT CTTCCACAGA GGAGTTGATG 
     3661 GACAATGGGA TCTGTCAAAC TATCACCTTT TAGACCTTGG ACGTCCTCAT CATTCCATAC 
     3721 GATGCATGAC TGTGGTACAT GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG 
     3781 TTCAACCAAA GGCTATGAAG ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC 
     3841 AAGTACGGCA GCTTGCATGG GTGGGTGATG GTGTATGGGT CTCCATTCGT TTGGATTCCA 
     3901 CGCTCCGCCT CTATCATGCA CACACATACC AACATCTCCA GGATGTGGAT ATTGAGCCTT 
     3961 ATGTAAGCAA AATGTTAGGT ACTGGAAAAC TGGGTTTCTC TTTCGTGAGG ATAACAGCTC 
     4021 TTATGGTGTC TTGCAATCGT TTGTGGGTGG GGACTGGAAA TGGTGTCATT ATCTCCATCC 
     4081 CACTGACAGA AACAAATAAA ACCTCAGGGA CTCCAGGTAA CCGTCCTGGA AGTGTAATCC 
     4141 GTGTATATGG TGATGAGAAC AGTGATAAAG TGACTCCAGG GACATTTATA CCCTACTGTT 
     4201 CAATGGCACA TGCACAGCTT TGTTTCCACG GGCACCGGGA TGCTGTGAAA TTCTTTGTGG 
     4261 CAGTCCCAGG TCAGGTCATT AGCCCACAAA GTAGCAGTGG TGGGGCAGAT CTTACAGCTG 
     4321 ACAAGGCAGG GTCATCTGCA CAGGAGCCCA GTAGCCAGAC GCCCTTGAAG TCCATGCTTG 
     4381 TCATCAGTGG AGGAGAGGGC TACATTGACT TCCGGATGGG TGATGAAGGT GGAGAATCTG 
     4441 AACTTCTGGG AGAGGATCTT CCACTGGAAC CTTCAGTCAC CAAAGCCGAG AGGAGTCACC 
     4501 TGATAGTGTG GCAAGTGATG TGTGGCAATG AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4561 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4621 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4681 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4741 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4801 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4861 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4921 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4981 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5041 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5101 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5161 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5221 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5281 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5341 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5401 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5461 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5521 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5581 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5641 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5701 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5761 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5821 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5881 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5941 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6001 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6061 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6121 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6181 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6241 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6301 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6361 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6421 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6481 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6541 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6601 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6661 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6721 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6781 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6841 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6901 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6961 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7021 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7081 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7141 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7201 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7261 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7321 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7381 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7441 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7501 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7561 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7621 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7801 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7861 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7921 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7981 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8041 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8101 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8161 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8221 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8281 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8341 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//