LOCUS       MR221137                8802 bp    DNA     circular  SYN 
DEFINITION  Spag9 (Myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 1
ACCESSION   NM_027569
VERSION     NM_027569.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4959..5050 
                     /label="Myc-DDK"  
     misc_feature    5080..5665 
                     /label="PolyA signal"  
     misc_feature    5137..5156 
                     /label="Restriction Enzyme"  
     misc_feature    5941..6613 
                     /label="ColE1"  
     misc_feature    7043..7837 
                     /label="Kan/Neo"  
     misc_feature    7872..8174 
                     /label="SV40 ori"  
     misc_feature    8260..8737 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4941..4947 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4941 
                     /label="Spag9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGGAG GACGGTGTGG TATACCAGGA GGAGCCCGGA GGCTCCGGGG 
     1081 CCGTGATGTC AGAGCGGGTG TCCGGCCTGG CCGGCTCCAT CTACCGCGAG TTCGAGCGGC 
     1141 TCATCGGGCG CTACGATGAG GAGGTGGTCA AGGAGCTGAT GCCACTGGTG GTGGCCGTGC 
     1201 TGGAGAACCT GGACTCGGTG TTCGCGCAGG ACCAGGAGCA CCAGGTGGAG CTGGAGCTGC 
     1261 TGCGGGACGA CAACGAGCAG CTCATCACCC AGTACGAGCG GGAGAAGGCG CTGCGCAAGC 
     1321 ACGCCGAGGA GAAATTCATT GAATTTGAAG ACTCTCAAGA ACAGGAAAAA AAGGATTTAC 
     1381 AGACGCGAGT GGAGTCTCTA GAATCTCAAA CAAGACAACT TGAACTCAAA GCAAAAAACT 
     1441 ATGCTGACCA GATCAGCAGA CTTGAAGAGA GAGAAGCAGA ACTGAAGAAG GAATATAATG 
     1501 CACTGCATCA GAGACACACT GAGATGATCC ATAATTATAT GGAACACTTA GAAAGAACCA 
     1561 AACTTCATCA GCTCTCAGGG AGTGATCAAC TAGAGGCTAC AGCTCATAGT AGAATTAGAA 
     1621 AAGAACGTCC TATATCATTA GGGATTTTCC CTCTACCTGC TGGAGATGGA TTGCTTACAC 
     1681 CTGACACTCA GAAAGGAGGC GAGACCCCAG GATCAGAGCA ATGGAAATTT CAAGAATTAA 
     1741 GTCAACCACG TTCTCATACC AGCCTGAAGG ATGAACTTTC TGATATTAGT CAAGGTGGAT 
     1801 CTAAAGCTAC CACTCCAGCT TCAACAGCAA ATTCAGACGT ATCAGCAATT CCTCCTGATA 
     1861 CTCCGTCAAA GGAAGATAAT GAAGGATTTG TAAAAGGCAC AGATACATCA AATAAGTCAG 
     1921 AGATAAGCAA ACACATAGAA GTCCAGGTTG CCCAAGAGAC TAGGAATGTG TCTACAGAAT 
     1981 CTGGTGAAAA TGAAGAGAAA TCAGAAGTTC AAGCAATCAT TGAATCCACT CCTGAATTGG 
     2041 ATATGGACAA AGACCTCAGT GGATATAAAG GTTCAAGCAC TCCCACCAAA GGCATAGAGA 
     2101 ACAAAGCTTT TGATCGCAAT ACAGAATCTC TCTTTGAAGA GCTGTCCTCA GCTGGCTCGG 
     2161 GCCTTATTGG AGATGTGGAT GAAGGAGCGG ATTTACTAGG AATGGGTCGT GAGGTGGAGA 
     2221 ATCTCATACT GGAGAACACA CAGCTGCTGG AAACCAAAAA CGCTCTGAAT GTAGTGAAGA 
     2281 ATGACTTAAT AGCCAAAGTG GATGAGCTGA CCTGTGAGAA GGACGTGCTG CAGGGGGAGC 
     2341 TGGAGGCTGT GAAGCAAGCC AAGCTGAAGC TGGAGGACAA GAACAGAGAG CTGGAGGAGG 
     2401 AGCTCAGGAA AGCTCGTGCA GAAGCTGAAG ATGCAAGGCA AAAAGCAAAA GACGATGATG 
     2461 ATAGTGACAT TCCCACAGCC CAGAGGAAGA GGTTTACCAG AGTAGAAATG GCCCGAGTTC 
     2521 TCATGGAGAG AAACCAGTAC AAAGAGAGGC TGATGGAGCT TCAAGAAGCT GTTCGATGGA 
     2581 CGGAGATGAT CCGGGCCTCA CGAGAGAACC CAGCTATGCA AGAAAAGAAA AGGTCAAGCA 
     2641 TCTGGCAATT TTTCAGCCGA CTTTTTAGCT CTTCAAGTAA TGCAACAAAG AAGCCTGAAC 
     2701 CCCCTGTTAA CCTGAAGTAC AACGCCCCCA CCTCTCACGT GACTCCCTCC GTGAAGAAAA 
     2761 GGAGCAGCAC TTTATCTCAG CTCCCTGGGG ACAAGTCCAA GGCATTTGAT TTCCTCAGTG 
     2821 AGGAAACTGA GGCTAGTTTA GCCTCCCGCA GAGAGCAAAA GAGAGAGCAG TATCGGCAGG 
     2881 TGAAGGCTCA CGTTCAGAAG GAAGATGGCC GAGTGCAAGC TTTCGGCTGG AGTCTGCCTC 
     2941 AGAAGTACAA ACAGGTAGCC AATGGTCAAG GGGAAACTAA GATGAAAAAT TTACCTGTGC 
     3001 CTGTCTATCT CAGACCACTA GACGAAAAAG ATGCGTCTAT GAAGCTGTGG TGTGCTGTAG 
     3061 GAGTCAATTT ATCTGGTGGC AAGACAAGAG ATGGCGGGTC TGTTGTTGGA GCGAGTGTAT 
     3121 TTTACAAGGA TATTGCTGGT TTGGACACTG AAGGCAGTAA ACAGCGGAGT GCGTCTCAGA 
     3181 GTAGTTTAGA CAAGTTGGAT CAAGAACTCA AGGAACAGCA GAAAGAATTT AAAAATCAAG 
     3241 AAGAGTTGTC CAGTCAGGTC TGGATCTGTA CCAGCACCCA CTCAACTACA AAGGTTATCA 
     3301 TCATTGATGC TGTTCAGCCT GGCAACATCC TAGATAGTTT CACTGTTTGC AATTCTCATG 
     3361 TTCTGTGCAT TGCCAGTGTC CCAGGAGCTC GGGAAACAGA CTACCCTGCA GGAGAAGAAC 
     3421 TTTCTGAATC TGGTCAGGTA GACAAAGCGT CATTGTGTGG AAGCATGACC AGTAACAGCT 
     3481 CCGCAGAGAT GGACAGCTTG CTGGGAGGCA TCACAGTGGT TGGGTGCTCC ACAGAAGGAC 
     3541 TGACAGGAGC TGCCACTTCC CCAAGTACCA ACGGTGCTTC TCCTGTGATT GAAAAGCCAC 
     3601 CAGAAATGGA AACTGAAAAT AGCGAAGTTG ATGAAAATAT TCCAACAGCA GAAGAAGCAA 
     3661 CTGAAGCCAC AGAGGGCAAT GCAGGGTCCA CTGAAGACAC TGTGGACATC TCCCAGCCTG 
     3721 GCGTGTACAC AGAGCATGTG TTTACAGATC CACTGGGAGT TCAGATCCCA GAAGACCTCT 
     3781 CCCCAGTGTT TCAGTCAAGT AATGACTCAG ATGTGTACAA AGATCAAATA TCAGTATTGC 
     3841 CAAATGAACA AGACCTTGCG AGAGAAGAAG CTCAGAAAAT GAGTAGTCTT TTACCAACCA 
     3901 TGTGGCTTGG AGCTCAGAAT GGCTGTTTGT ATGTCCACTC ATCTGTAGCC CAGTGGAGGA 
     3961 AATGTCTCCA TTCCATTAAG CTCAAAGACT CAATTCTCAG TATTGTACAT GTGAAAGGAA 
     4021 TTGTACTAGT GGCCCTGGCG GATGGCACCC TTGCAATCTT CCACAGAGGA GTTGATGGAC 
     4081 AATGGGATCT GTCAAACTAT CACCTTTTAG ACCTTGGACG TCCTCATCAT TCCATACGAT 
     4141 GCATGACTGT GGTACATGAC AAAGTCTGGT GTGGCTATAG GAACAAAATC TATGTGGTTC 
     4201 AACCAAAGGC TATGAAGATA GAGAAATCAT TTGATGCACA CCCCAGGAAG GAGAGCCAAG 
     4261 TACGGCAGCT TGCATGGGTG GGTGATGGTG TATGGGTCTC CATTCGTTTG GATTCCACGC 
     4321 TCCGCCTCTA TCATGCACAC ACATACCAAC ATCTCCAGGA TGTGGATATT GAGCCTTATG 
     4381 TAAGCAAAAT GTTAGGTACT GGAAAACTGG GTTTCTCTTT CGTGAGGATA ACAGCTCTTA 
     4441 TGGTGTCTTG CAATCGTTTG TGGGTGGGGA CTGGAAATGG TGTCATTATC TCCATCCCAC 
     4501 TGACAGAAAC AAATAAAACC TCAGGGACTC CAGGTAACCG TCCTGGAAGT GTAATCCGTG 
     4561 TATATGGTGA TGAGAACAGT GATAAAGTGA CTCCAGGGAC ATTTATACCC TACTGTTCAA 
     4621 TGGCACATGC ACAGCTTTGT TTCCACGGGC ACCGGGATGC TGTGAAATTC TTTGTGGCAG 
     4681 TCCCAGGTCA GGTCATTAGC CCACAAAGTA GCAGTGGTGG GGCAGATCTT ACAGCTGACA 
     4741 AGGCAGGGTC ATCTGCACAG GAGCCCAGTA GCCAGACGCC CTTGAAGTCC ATGCTTGTCA 
     4801 TCAGTGGAGG AGAGGGCTAC ATTGACTTCC GGATGGGTGA TGAAGGTGGA GAATCTGAAC 
     4861 TTCTGGGAGA GGATCTTCCA CTGGAACCTT CAGTCACCAA AGCCGAGAGG AGTCACCTGA 
     4921 TAGTGTGGCA AGTGATGTGT GGCAATGAAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4981 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5041 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5101 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5161 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5221 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5281 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5341 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5401 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5461 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5521 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5581 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5641 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5701 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5761 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5821 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5881 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5941 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6001 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6061 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6121 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6181 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6241 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6301 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6361 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6421 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6481 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6541 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6601 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6661 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6721 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6781 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6841 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6901 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6961 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7021 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7081 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7141 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7201 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7261 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7321 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7381 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7441 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7501 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7561 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7621 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7681 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7741 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7801 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7861 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7921 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7981 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8041 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8101 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8221 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8281 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8341 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8401 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8461 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8521 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8581 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8641 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8701 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8761 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//