LOCUS       MR221109                8259 bp    DNA     circular  SYN 
DEFINITION  Hps5 (Myc-DDK-tagged) - Mouse Hermansky-Pudlak syndrome 5 homolog (human) (Hps5), transcript variant 1
ACCESSION   NM_001005247
VERSION     NM_001005247.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4416..4507 
                     /label="Myc-DDK"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    4594..4613 
                     /label="Restriction Enzyme"  
     misc_feature    5398..6070 
                     /label="ColE1"  
     misc_feature    6500..7294 
                     /label="Kan/Neo"  
     misc_feature    7329..7631 
                     /label="SV40 ori"  
     misc_feature    7717..8194 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4398..4404 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4398 
                     /label="Hps5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTTTTGTG CCAGTGATAC CAGAAGCCTA CAGCCATGTT CTTGCAGAGT 
     1081 TTGAGTCCTT GGATCCGTTA CTCACAGCCC TGAGGCTGGA CTCCAGTCGT CTGAGGTGTA 
     1141 CCAGCATAGC TGTGTCTCGG AAATGGTTGG CGTTAGGCAG CACAGGAGGA GGACTCAATC 
     1201 TCATTCAGAA AGATGGCTGG AAGCAAAGGC TGTTTCTCTC TCACCGGGAA GGGGCAATCT 
     1261 CTCAGATTGC CTGCTGCTCT CATGATGATG ATTATGTTGC TGTAGCTACC AGTCAAGGTC 
     1321 TTGTAGTTGT TTGGGAATTA AATCAAGAGC GTCGTGGGAA GCCAGAACGA ATTCATGTGT 
     1381 CCTCAGAACA CAAAGGCCGA AAAGTTACTG CCCTCTGTTG GGACACGGCT GTTCTGAGAG 
     1441 TCTTTGTCGG TGACCATGTG GGCAAAGTAT CTGCCATCAA ACTCAACACT TTGAAACAAG 
     1501 CAAAAGCAGC TGCTGCCTTT GTGATGTTTC CTGTGCAGAC AGTACTAACA GTGGACTCGT 
     1561 GTGTCGTCCA GTTGGATTAC TTGGATGGAA GATTACTTGT GTCTTCACTG ACTCGATCCT 
     1621 TCTTGTGTGA CACCGAGAGG GAAAAGTTTT GGAAAATCGG AAATAAGGAA AGACATGGAG 
     1681 AATATGGGGC TTGTTTCTTC CCCGGGCGGT GTGCTGGGGG CCAGCAGCCT GTGATCTACT 
     1741 GCGCCCGCCC AGGCTCCCGG ATGTGGGAAG TGAACTTTGA CGGGGAAGTG CTCAGTACAC 
     1801 ACCAGTTCAA GAAGCTCCTG TCAATGCCAC CCCTCCCTGT GATCACTGCA AGATCAGAGC 
     1861 CTCAGTATGA TCACACAGTT GGATCCTCCC AGTCTTTGGC ATTCCCCAAA CTCTTGCATC 
     1921 TTAGTGAACA CTGTGTGCTG ACTTGGACAG AAAAGGGAAT TTATATTTTC ATTCCTCAGA 
     1981 ATGTTCAAGT TCTTCTTTGG AGTGAAGTCA AGGATATTCA GGATGTAGCT GTCTACAAGA 
     2041 ATGAATTGTT CTGTTTACAC TTTAATGGAA AAATCTCACA TCTTTCCCTG TTGTCTGTGG 
     2101 AGCGCTGTGT AGAACGCCTG TTAAGGAGAG GCCTGTGGGA CCTGGCTGCT CGCACATGCT 
     2161 GTCTCTTCCA AAATTCTATT ATTACCAGCA GAGCAAGAAA AACATTGACT GCAGATAAGT 
     2221 TGGAGCATTT GAAGTCTCAG CTGGACCTTA CAGCCTGCAG TGAACTAATT TCTCAGCTGG 
     2281 ACGACTTGAT CTTAAGGTTC GAGCCTTTGG AGTCGGCGTG CAGCAGCAGA AGAAGCTCCA 
     2341 TCTCATCGCA TGAAAGTTTT AGCATCTTGG ACTCTGGTAT TTATCGTATC ATCAGTAGTA 
     2401 GAAGAGGCAG TCAGTCCGAC GAAGATTCTT GTTCCCTTCA CAGCCAGACC TTCTCAGAAG 
     2461 ATGAAAGGCT TAAAGAATTT GCCTCACACC AGGAAGAGGA GCAGCCAGAG CAGGGCTGTG 
     2521 GTGCCAATAG GAATGAAGAG AGTGCTTCCC ACAGTCCAGT GATGTCTGAG GTAGATAAGA 
     2581 GTGAAGCTTT TCTGCCCTTC AGCATTGCAC TGCCATTTCG TTCTCCATCT CCCCTCGTGT 
     2641 CTCTTCAGGC TGTTAAAGAC AGTGTTTCTA GCTTTGTGCG TAAAACTACT GAGAAGATTG 
     2701 GCACCCTTCA CGGAAGCCCC GAGCTGAAAG AGCCTTTTGA ATCCAAGGAT GCTGACCGAG 
     2761 CACATGAAGA GGAAGTGAGT GCAGTCACCT GCCCTCTGGA GGAGGACACT GAGGAGAAAG 
     2821 AAATTCATCA ACCTCCTAAA GAAGACAGGC TTCAAGAACT CACAGCAGCG ACAGCAGAAG 
     2881 CAATGACCAA GTTATTGGAC CCTCTGGTTC TGTTTGAGCC CAAGGTGCTA AGAATGGTTT 
     2941 TACTTGAGTG GCTTTCACAG TTAGAGAAAA CATTTGCCAT GAAAGACTTC CCCGGCATTT 
     3001 CCAATACCAG CAGTCCAACC GTGAAATCAA ACTTGGGTGC ACACCTCCTC GGTGAGACAG 
     3061 AAAAGAGAGT ATTAGATGAA GAGAGTGGAG AAGGAAGAAG GGTCTCTTTA GTCACTGAAG 
     3121 AAGCTGGAGG TCAGATAACC TGTGACCCTG TAAGCAACCT CAGTGAGCCC TCGGCTGACC 
     3181 GTTTTCGAGT ATGCTCTCCA TACGCCATCA CAAACAGCCT TCAGAGGGAC CTGGCGGAAT 
     3241 TGACAACGTT GTGCTTGGAA CTGAATGTGT TGACTTCAGC AATGGAAAGC GTAGGTGGGC 
     3301 ATGTGGACCG GGCTTCACAG CAGCTCTCTC CAGAAATCCT GGCTTGTCGT TTCCTAAAGA 
     3361 AGTACTTTTT TCTTCTGGAC TTGAAAAGAG CAAAGGAGAG TATCAAGCTC ACTTACGACA 
     3421 GCCCTTGCGT TTGGGACACT TTTGTCGAAG GACTAAAAGA AATGGCAAGA TCTAATCCTG 
     3481 CCTACACGGA GCTGGAAGAA GGAGACCTGC CCACGGGGTT ACAGTTACTG GATGGCTCCG 
     3541 TCCCTTCTGA TAGTCCCTTG TTGATTGCAT TTGCCACCCG GTTGTATGAT AGATTTGGGG 
     3601 AGTCAGCCCT TCGAGCTTGC ATCAAGTTTT ATCCGTCCAT TTCGCCCTCC GACATCGCAC 
     3661 AGCTTTGCCG TCATCATCCT GCTCAGTTCT TGGCCTATCT AGACAGTCTG GTGAAGTCCA 
     3721 GGCCGGAAGA CCAGTGGCCA TCCTTCCTTG AGTTCCTTCT ACAACCAGAG TCTTTAAGAC 
     3781 TTGAATGGCT GCTTTTGGCA GTGTCCCATG ATGCTCCACC AAGCACAAGT ACAGTGGATG 
     3841 ATGAAGGGCA CCCCAGGCCC CATTCACACT TGCTTTCTTG GGGTTATAGT CAGCTGATTC 
     3901 TTCTTCTTAT TAAACTTCCT GCAGACTTTA CAACCAAAGA GAAAATGACT GACATCTGTA 
     3961 GGTCTTACGG TTTCTGGCCT GGATATCTTA CCCTCTGTCT GGAGCTGGAG AGGAGGAGGG 
     4021 AGGCCTTCAC CAACATTGTG TATCTGAATG ACATAAGCCT GATGGAAGGG GACAATGGGT 
     4081 GGATCCCTGA GACCTTGGAG GAATGGAAGC TTCTCCTACA TCTACTACAG ACCAAGAGCA 
     4141 CAAGGCCAGC TCCCCAGGAG TCACTAAACG GGAGCCTCAG TGATGGGCCA GCCCCTATCA 
     4201 ATGTGGAGAA TGTGGCCCTC CTGTTAGCTA AGGCCATGGG CCCAGATCGG GCCTGGTCAC 
     4261 TGCTACAGGA GTGTGGTCTG GCTCTTGAGT TGTCAGAAAA GTTCACCAGA ACCTGTGATA 
     4321 TCCTGAGGAT TGCTGAGAGA AGGCAGAGAG CGCTGATACA AGGCATGCTT GAGAAGTGTG 
     4381 ACCGCTTCCT CTGGTCGCAG CAGGCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4441 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6061 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6121 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6181 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6241 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6301 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6361 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6421 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6481 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6541 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6601 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6661 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6721 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6781 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6841 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6901 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6961 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7021 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7081 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7141 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7201 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7261 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7321 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7381 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7441 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7501 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7681 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7801 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7861 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7921 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7981 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8041 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8101 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8161 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8221 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//