LOCUS       MR221085                8541 bp    DNA     circular  SYN 
DEFINITION  Atp2b1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1)
ACCESSION   NM_026482
VERSION     NM_026482.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4698..4789 
                     /label="Myc-DDK"  
     misc_feature    4819..5404 
                     /label="PolyA signal"  
     misc_feature    4876..4895 
                     /label="Restriction Enzyme"  
     misc_feature    5680..6352 
                     /label="ColE1"  
     misc_feature    6782..7576 
                     /label="Kan/Neo"  
     misc_feature    7611..7913 
                     /label="SV40 ori"  
     misc_feature    7999..8476 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4680..4686 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4680 
                     /label="Atp2b1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACATG GCCAACAACT CAGTTGCGTA TAGCGGTGTG AAGAACTCTT 
     1081 TGAAAGAAGC TAACCATGAT GGAGACTTTG GAATTACGCT TACAGAGCTG CGAGCTCTCA 
     1141 TGGAGCTCAG ATCCACGGAT GCATTACGGA AAATACAGGA GAGCTATGGA GATGTCTATG 
     1201 GCATTTGCAC CAAGTTGAAA ACATCTCCCA ATGAAGGTTT AAGTGGAAAT CCTGCAGATT 
     1261 TAGAAAGAAG AGAAGCAGTG TTTGGAAAGA ACTTTATACC TCCTAAGAAG CCAAAAACCT 
     1321 TTCTTCAGTT AGTCTGGGAA GCATTACAAG ATGTCACTTT AATTATACTA GAAATTGCAG 
     1381 CCATAGTATC ATTGGGCCTT TCTTTTTATC AACCTCCGGA AGGGGATAAT GCACTTTGTG 
     1441 GAGAAGTTTC TGTTGGGGAA GAAGAAGGTG AAGGAGAAAC GGGGTGGATT GAAGGAGCTG 
     1501 CGATCCTCTT GTCGGTGGTG TGTGTGGTGT TGGTGACGGC TTTCAACGAC TGGAGCAAGG 
     1561 AGAAGCAGTT CCGAGGCCTG CAGAGTCGAA TTGAGCAGGA ACAGAAGTTC ACCGTCATCA 
     1621 GGGGTGGGCA GGTCATCCAG ATACCTGTAG CTGACATTAC TGTTGGAGAC ATTGCCCAAG 
     1681 TGAAGTACGG TGACCTTCTT CCAGCTGATG GCATACTGAT TCAAGGCAAT GACCTGAAGA 
     1741 TTGATGAGAG CTCATTGACT GGTGAATCTG ATCATGTTAA GAAGTCTCTA GATAAGGATC 
     1801 CCTTACTTCT TTCAGGTACT CATGTGATGG AAGGTTCTGG AAGAATGGTG GTCACTGCTG 
     1861 TAGGTGTGAA TTCTCAAACA GGAATCATCT TCACCTTACT TGGAGCTGGG GGTGAAGAAG 
     1921 AGGAAAAGAA AGATGAGAAG AAGAAGGAAA AGAAAAATAA GAAACAAGAT GGAGCTATTG 
     1981 AGAATCGCAA CAAAGCAAAA GCCCAGGACG GCGCGGCCAT GGAGATGCAA CCTCTGAAGA 
     2041 GTGAAGAAGG AGGGGACGGT GATGAGAAAG ACAAGAAGAA AGCAAATCTG CCAAAAAAGG 
     2101 AAAAATCCGT GTTACAGGGG AAACTCACAA AGCTGGCTGT TCAGATTGGC AAAGCAGGTC 
     2161 TGCTGATGTC TGCCATCACA GTTATCATCC TAGTGTTGTA TTTTGTGATC GATACCTTCT 
     2221 GGGTTCAGAA AAGACCATGG CTTGCAGAGT GCACACCGAT CTACATCCAG TACTTCGTGA 
     2281 AGTTCTTCAT TATTGGAGTC ACAGTTCTAG TGGTCGCCGT GCCGGAAGGT CTTCCGCTGG 
     2341 CAGTCACCAT CTCACTGGCT TACTCAGTCA AGAAAATGAT GAAGGATAAT AACTTAGTGA 
     2401 GACATCTGGA TGCTTGTGAG ACCATGGGGA ATGCTACAGC TATCTGTTCA GATAAAACAG 
     2461 GAACCCTGAC GATGAACAGG ATGACGGTTG TTCAAGCTTA CATAAATGAA AAGCATTATA 
     2521 AAAAGGTTCC GGAACCAGAA GCCATCCCAC CAAATATCCT GTCCTACCTG GTAACGGGGA 
     2581 TTTCTGTGAA TTGTGCTTAT ACGTCAAAAA TATTGCCACC GGAGAAAGAG GGTGGATTAC 
     2641 CCCGCCATGT GGGTAACAAA ACAGAGTGTG CCTTGTTGGG GTTTCTCTTG GATTTAAAAC 
     2701 GGGATTATCA AGATGTTAGA AATGAAATAC CAGAGGAAGC ACTGTACAAA GTCTATACCT 
     2761 TCAATTCTGT TAGGAAGTCC ATGAGCACAG TCCTGAAAAA CTCAGATGGA AGTTTCCGCA 
     2821 TCTTTAGCAA GGGTGCCTCC GAGATCATTC TGAAAAAGTG TTTCAAAATC CTGAGTGCTA 
     2881 ATGGCGAGGC AAAAGTATTC CGACCCAGAG ACCGCGATGA CATTGTGAAA ACTGTGATCG 
     2941 AGCCCATGGC ATCAGAAGGC CTGAGGACCA TATGCCTAGC GTTCAGAGAC TTCCCAGCGG 
     3001 GGGAGCCAGA ACCAGAGTGG GATAATGAGA ATGATGTCGT CACCGGCCTT ACGTGTATCG 
     3061 CGGTTGTGGG GATTGAAGAT CCTGTGAGGC CTGAGGTACC AGAGGCAATA AAGAAATGTC 
     3121 AGAGGGCTGG AATTACTGTG CGCATGGTCA CTGGTGATAA TATTAACACT GCTCGGGCCA 
     3181 TTGCTACCAA ATGTGGTATT TTACACCCAG GGGAAGATTT CCTATGCTTA GAAGGTAAAG 
     3241 ATTTTAACCG AAGAATACGA AATGAAAAAG GAGAGATTGA ACAAGAAAGG ATAGACAAGA 
     3301 TTTGGCCAAA GCTTCGAGTA CTTGCAAGAT CATCTCCCAC TGACAAACAT ACACTGGTGA 
     3361 AAGGTATAAT TGACAGCACA GTCTCAGAGC AACGACAGGT TGTAGCTGTA ACGGGTGATG 
     3421 GTACAAATGA CGGGCCTGCA CTGAAGAAAG CAGACGTTGG CTTTGCAATG GGCATTGCTG 
     3481 GAACTGATGT GGCTAAAGAA GCGTCTGATA TTATTCTCAC AGATGACAAC TTTACAAGCA 
     3541 TTGTCAAAGC AGTTATGTGG GGACGAAATG TCTATGACAG CATCTCAAAA TTCCTTCAGT 
     3601 TCCAGCTTAC TGTTAATGTA GTAGCAGTGA TTGTTGCTTT CACGGGCGCT TGTATTACTC 
     3661 AAGACTCGCC ACTTAAGGCG GTGCAGATGC TCTGGGTAAA CCTCATCATG GACACGCTGG 
     3721 CTTCCCTGGC TCTGGCTACG GAACCACCCA CCGAGTCACT CCTGCTTCGG AAGCCTTATG 
     3781 GTAGAAATAA GCCTCTCATC TCACGCACGA TGATGAAGAA CATCTTGGGC CATGCATTCT 
     3841 ATCAGCTCGT AGTGGTCTTT ACACTCTTAT TTGCTGGAGA AAAGTTTTTT GATATCGACA 
     3901 GCGGGAGGAA TGCGCCTCTG CACGCTCCCC CGTCAGAACA TTACACCATC GTGTTCAATA 
     3961 CCTTTGTGCT GATGCAGCTC TTCAATGAAA TAAACGCCCG GAAAATTCAC GGGGAAAGAA 
     4021 ATGTGTTTGA AGGAATCTTC AATAACGCCA TCTTCTGCAC CATTGTCCTG GGCACCTTTG 
     4081 TGGTGCAGAT AATAATTGTG CAGTTTGGCG GGAAGCCTTT CAGCTGCTCA GAACTTTCAA 
     4141 TAGAGCAATG GCTGTGGTCG ATATTCCTGG GAATGGGGAC CTTACTCTGG GGCCAGCTTA 
     4201 TTTCAACCAT TCCAACCAGC CGCTTAAAGT TTCTAAAAGA AGCTGGTCAT GGAACCCAGA 
     4261 AGGAGGAGAT ACCTGAGGAG GAATTAGCAG AGGATGTTGA AGAGATTGAC CATGCCGAAA 
     4321 GGGAGTTGCG GCGTGGCCAG ATCTTGTGGT TTAGAGGCCT GAACAGAATC CAAACACAGA 
     4381 TCCGAGTGGT GAATGCATTT CGTAGTTCTT TATACGAAGG GTTAGAGAAG CCAGAATCAA 
     4441 GAAGTTCAAT CCACAACTTT ATGACACACC CTGAGTTTAG GATAGAAGAT TCAGAGCCGC 
     4501 ACATCCCCCT TATTGATGAC ACTGATGCTG AAGACGACGC CCCAACAAAA CGCAACTCCA 
     4561 GCCCTCCGCC CTCTCCTAAC AAAAATAACA ATGCTGTTGA CAGCGGAATT CACCTTACAA 
     4621 TAGAAATGAA CAAGTCTGCT ACCTCTTCAT CCCCAGGAAG CCCACTACAT AGTTTGGAAA 
     4681 CATCACTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//