LOCUS       MR221073                7875 bp    DNA     circular  SYN 
DEFINITION  Tmem67 (Myc-DDK-tagged) - Mouse transmembrane protein 67 (Tmem67)
ACCESSION   NM_177861
VERSION     NM_177861.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4030..4121 
                     /label="Myc-DDK"  
     misc_feature    4151..4736 
                     /label="PolyA signal"  
     misc_feature    4208..4227 
                     /label="Restriction Enzyme"  
     misc_feature    5012..5684 
                     /label="ColE1"  
     misc_feature    6114..6908 
                     /label="Kan/Neo"  
     misc_feature    6943..7245 
                     /label="SV40 ori"  
     misc_feature    7331..7808 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4005..4012 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4005 
                     /label="Tmem67"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTACCATG GTGACGCGTA CAAGGCCTGT GGCGGCCATG GCGGTCAGAT 
     1081 CTCGTTCTTC ATCCCGGACT GGCACCGCCT ACCTTCTCTT AGTTCTCTGC GAAGTCTCAT 
     1141 GGGCACAAAT CTTCTCCTTC CCTTTCCGGC GGCCTGAGAC CTGCGACTTC AACCAGTACT 
     1201 TTGACATCTC GGCGCTCTCC TGTGCCCCCT GTGGCGCCAA CCAGCGGCGG GATGCACTAG 
     1261 GAACTTCCTG TGTATGTCTA CCAGGATATC ATATGATCTC TAATAATGGA GGACCTTCTA 
     1321 TTATTTGTAA AAAGTGTCCA GAAAACATGA AAGGCGTTAC TAAAGATGGC TGGGACTGCA 
     1381 TTTCTTGTCC TAGTGGCTTA ACTGCTGAGG GGAAGTGCCA CTGTCCCACT GGCCATATAT 
     1441 TAGTGGAAAG AAATGTTAGT GGGTCTTTGT TAGCCCAAGC AACTTGCGAG CTTTGTGATG 
     1501 AAAGTGAAAA CTCATTTACA AAAGCCAATG CTTTAGGAAC CAGGTGTGTC CGCTGCGAAC 
     1561 CAACATTTGT GAATACAAGC AGGTCCTGTT CATGTTCAGA GCCTCACACT TTAACAGGTG 
     1621 GATTATGTTT CAGCAACACA GGGAATTTTC ATCAACGTGT AATTTCCACT GCACGTTATG 
     1681 GAGAGCTTGG CATGTCATTA AATTCAGAAT GGTTTGCCAA GTATTTGCAG GCAACAGCAG 
     1741 CTGCATGCTG GACTCACGCC AACCTGACAT CGTGCCAAGC TCTTGGAAAT ATGTGCGTGA 
     1801 TGAACATGAA CTCTTACGAC TCGACCACAC TCGATGCTTG TCGACTGTTC CATTACATCT 
     1861 TTGAAAGCAC TGCTGGATTG ATCAGTGTGC ATTCTGTTCC ATTCTGGAGA CAGAACCTTC 
     1921 CTTGGCTGTT CTATGGAGAC CAGCCAGGAT TGGCTCCTCA AGTTCTCAGT ACTACACCTC 
     1981 TTCCTACAAA TTTCAGTTTT AAAGGACAAA ACCAGCTGAA GTTTGTTGCT GCTTCCTATG 
     2041 ATATACGAGG AAACTTCATC AAATGGCAAC CTTTAGAAGG TGGTGTTTTA CAGCTTTGTC 
     2101 CAGACACTGA GAGAAGGCTG GATGCAGCGT ATGCTTTTGG AACAACGTAC CAGCAAAATT 
     2161 GTGAAATCTC TCTCTCTAAA CTCCTGGTTG ACTTCAGCAG CCCTGTATTT TATGATGTTT 
     2221 ACCTCGAATA CACTGATGAA GAGCAGCACC GTTACCTTTG GCCTATCCCA GTGTTAAACC 
     2281 TAAATCTTCA ACACAACAAA CTGTTTGTGA ACCAAGACAG TAGCTCCAGC AAGTGGCTTC 
     2341 TGACTCGGCG CATTTTCTTA GTGGATGCAG TAAGTGGGCG AGAAAATGAC CTAGGAAATC 
     2401 AGCCAAGAGT AATTCGAGTT GCTACCCAGA TTTCACTGAG CATACGCCTT GTACCCAACA 
     2461 CAAAAAATGG AAACATATAC ACTCCCCTAC TTACCATTGC CTACAGTGAC ATTGACATCA 
     2521 AAAATGCTCA TAGCCAGTCT GCGAAGATTT CCTTCTCAGT GAAGTATGAG ATGAATCAAG 
     2581 GAGACGCATC TGTGCACACA GATATTGCGT TGGGTGTGTT GGGTGGGCTG GCTGTGCTAT 
     2641 CATCTCTTTT GAAGACAGCG GGATGGAAGA GGCGCATCGG GAGCCCCATG ATTGATTTAC 
     2701 AGACAGTTAT GAAATTCCTG CTGTACTATG CTGGTGATCT GGCCAATGTG TTTTTTATCA 
     2761 TCACAGTGGG AACAGGTCTT TATTGGCTTA TTTTCTTCAA AGCACAGAAG TCAGTCTCTG 
     2821 TTTTGCTGCC GATGCCAGTT CAGGAAGAAC GTTTCGTTAC TTATGTGGGG TGCGCCTTTG 
     2881 CTATGAAGGC TCTCCAGTTT TTACATAAGT TCATATCCCA GATTTCCATA GATATATTCT 
     2941 TTATTGATTG GGAACGGCCG AAAGGAAAAG TTCTTAAAGC TGTTGAAGGT GAGGGTGGTG 
     3001 TGCGGAGTGC CACTGTTCCT GTGAGCATAT GGAGAACGTA TTTCGTGGCA AATGAATGGA 
     3061 ATGAGATTCA GACTGTGAGA AAAATTAATC CACTCTTTCA AGTGCTTACC ACACTCTTCT 
     3121 TCTTGGAGGT TGTGGGATTC AAAAATTTAG CGTTAATGGA TTCATCCTCC AGCCTTTCTA 
     3181 GAAACCCATC AGACTACACA GCTCCTTACA GCCGCATTCT GAGATATGCC GTGGCTACTG 
     3241 CTATCTGGCT GGTGATTGGA ATAATACAGG TTGTTTTCTT TGCTGCCTTT TATGAGAGAT 
     3301 TCATAGAAGA CAAAATCCGG CAGTTTGTTG ACCTGTGCTC CATGAGTAAT GTGTCAGTGT 
     3361 TCCTGCTGTC CCACAGATGT TTTGGATATT ACATTCATGG GAGGTCAGTC CACGGCCATG 
     3421 CAGACACTAA CATGGAGGAA ATGAATATGA ACCTTAAGAG AGAGGCGGAA AATTTGTGCA 
     3481 GCCAAAGAGG TTTGGTGCCC AACACGGATG GGCAGACGTT TCAGATTGCA GTTTCTAGTC 
     3541 AGATGAGACA ACACTATGAC AGGATTCATG AGACTTTAAC AAGGAGAAAT GGCCCTGCCA 
     3601 GACTGCTGAG TTCGTCGGGG AGTACTTTTG AACAAAGCAT CAAAGCATAC CACGCAATGA 
     3661 ATAAGTTCCT TGGCTCTTTC ATTGACCACG TTCATAAGGA AATGGATTAC TTTATAAAAG 
     3721 ATAAGTTGCT TCTTGAAAGA ATCCTTGGGA TGGAATTCAT GGAGCCCATG GAGAAGAGCA 
     3781 TCTTCTATAA TGATGAAGGC CATTCTTTCA GCAGTGTGCT GTATTATGGG AACGAGGCCA 
     3841 CGCTCCTCAT CTTTGATCTG TTGTTCTTCT GTGTTGTGGA TTTGGCTTGC CAGGACTTTG 
     3901 TTTTAGCATC CTTCCTCACA TACCTGCAAC AAGAGATTTT CCGATTTATC CGAAATACAG 
     3961 TAGGACAAAA GAATTTGGCA ACCAAAACAC TAGTGGATGA AAGATTTCTG ATCAGCGGAC 
     4021 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4081 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4141 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4201 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4261 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4321 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4381 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4441 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4501 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4561 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4621 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4681 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4741 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4801 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4861 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4921 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4981 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5041 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5101 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5161 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5221 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5281 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5341 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5401 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5461 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5521 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5581 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5641 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5701 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5761 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5821 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5881 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5941 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6001 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6061 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6121 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6181 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6241 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6301 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6361 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6421 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6481 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6541 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6601 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6661 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6721 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6781 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6841 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6901 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6961 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7021 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7081 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7141 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7201 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7261 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7321 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7381 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7441 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7501 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7561 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7621 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7681 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7741 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7801 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7861 TTTAACGCGA ATTTT
//