LOCUS       MR221067                8319 bp    DNA     circular  SYN 
DEFINITION  Tacc2 (Myc-DDK-tagged) - Mouse transforming, acidic coiled-coil containing protein 2 (Tacc2), transcript variant 1
ACCESSION   NM_206856
VERSION     NM_206856.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="Tacc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACGAG AACAGCACCT CGGACCACCA GAGGACTTCT TCAGTTCAGA 
     1081 GTCCAAGGTC ACTGCAGCCA CCTGGGAAGA GTCAATCTCT ACAGAAGCAA CAAGGAGACT 
     1141 TGCCTGGATC GTGTGCTGGA AGTATCCCTG GAACTGATGA TGTCATCCAG CCAGCTGCTC 
     1201 CTGTTGACCC GGGACACCCA CCCTTAGCTG ACTCGTCCCA CCATGGTGAC GCTGTCAGCT 
     1261 CAGTCTCTAC ACATCTGACA GTCCAGAGCG CCTCCCCATC TGCTGCCCGT GCAAGTCCTG 
     1321 CTCCTTTGGC CCCAGAACAT ACAGCCTCGG CTCCCTCCGC AGCCGGTCCT GGAGTAGAAG 
     1381 TCACCCCCAC TGCCTCCCCC CAACACCTGG CCAAGAACGA ACCCAGGAGT TCCGACTCTG 
     1441 AGGAAGCCTT TGAGACCCCC GAGTCAACGA CCCCTGTCAA AGCTCCACCA GCCCCTCCCC 
     1501 CTCCACCCCC TGAAGTCACC CCAGAGCCTG AGGTCATCGA TCCACCAGCC CCAGAAGAAC 
     1561 CAGGATGCAT TTCTGAGCCA CCGGTTGTGG TTCCCGATGG CCCTCGCAGC AGCGAGTCCG 
     1621 TGGAAGGAAG CCCTTTCCGT CCTTCCCACT CCTCCTCGGC GGTGTTCGAT GAAGACAAGC 
     1681 CGATAGCCAG CAGCGGGACT TACAACTTAG ACTTCGACAG CATCGAGCTG GTGGATAACT 
     1741 TCCAGAGCTT GGAGCCGTGC TCTGCCGACT CTAAGGGTCA GGAGTGTAAG GTGAGCACGC 
     1801 GGAGGAAATC CACCGAGTCC GTGCCACCCT CCAAGTCCAC GCTGTCCCGT TCGCTTAGCC 
     1861 TACAAGCCAG TGACTTTGAC GGTGCTTCTT GCCCCGGCAG CCCTGAAGCT GGGACCCTTA 
     1921 CCACAGATGC ATGTGGCACA GGATCCAACA GTGCTTCCAG CACCCTTAAG CGAACTAAAA 
     1981 AAACACGGCC ACCTTCCTTG AAAAAGAAGC AAGCCACCAA GAAACCCACA GAAACCCCCC 
     2041 CAGTGAAGGA GACCCAACAG GAACCAGGTG AAGAGAGTCC AGTCCCCAGT GAGGAACACT 
     2101 TAGCACCAGA GACAAAGACG GAGTCGGCCA CACCTGAGGG CGCTGGTTGC ACCTTGTCAG 
     2161 ACGATACACC CCTGGAGTCC CCTGCTGTCC CCACAGCCAC CTGTCCTCTG ACTTTGGAGA 
     2221 GTGCAGAAGA CGTTAGCCCC CTGGTTTCTG GAGGTGGCAG AGTGCAGAAC TCACCCCCAG 
     2281 TTGGGAGGAA ATCAGTGCCT CTTACCACAG CCTCTGAGGC GGTGGAGGTG ACCCTGTCGG 
     2341 ACAGTGGGGG GCAAGAGGAC TTGCCTGCCA AGGGGTTGTC AGTGAGGCTG GAGTTTGACT 
     2401 ATTCTGAGGA CAAGGGAAGT TGGGAGAGTC AGCAGGAAAA CGCCCCTCCC ACCAAAAAGA 
     2461 TAGGCAAGAA GCCAGTTGCC AAAATGCCCC TAAGGAGGCC AAAGATGAAA AAGACCCCCG 
     2521 AGAAACTTGA CAACACTCCT GCCTCACCTC CAAGGTCCCC TACTGAACCC AGTGACACCC 
     2581 CTATTGCTAA AGGTACCTAC ACCTTTGATA TAGACAAGTG GGATGACCCC AATTTTAACC 
     2641 CTTTTTCCTC CACCTCAAAA ATGCAAGAGT CTCCCAAACT GTCCCAACAA TCGTACAACT 
     2701 TTGACCCTGA CGCCTGTGAA GAGTCCCTTG ACCCCTTTAA GGCATCCTCT AAGACCCCCA 
     2761 GCTCACCTTC TAAGTCCCCA GCCTCTTTTG AGATCCCAGC CAGCACCACG GAAGCGGATG 
     2821 GGGATGGGTT GAATAAACCT GCCAAGAAGA AGAAGACTCC ACTAAAGACG ATGGTTGAAG 
     2881 ATGTGATGTC TGTGTGTTCT CTGTTTGACA CGTTTAGGGT GAAGAAGTCT CCAAAGCGGT 
     2941 CTCCTCTGTC TGACCCGCCT TCTCAGGACC CCACTCCAGC TGCCACGCCG GAAGCACCCT 
     3001 CAGCCATCTC TACAGTGGTC CACGCCACAG ATGAGGAGAA GCTGGCAGTC ACTAGCCAGA 
     3061 AGTGGACGTG TATGACGGTG GACTTGGATG CTGACAAACA GGACTTCCCT CAGCCCTCGG 
     3121 ACCTGTCTAA CTTTGTAAAT GAGACCAAAT TCAATTCACC CTCAGAGGAG CTGGACTACA 
     3181 GAAACTCCTA TGAAATTGAA TACATGGAAA AGCTTGGCTC CTCCTTACCT CAGGACGATG 
     3241 ACACTCCGAA GAAGCAGGCC TTGTACCTTA TGTTTGACAC TCCTCAGGAG AGCCCCGTCA 
     3301 AGTCTCCTCC AGTCCGGATG TCAGATTCCC CAACTCCATG TTCAGGGTCG AGTTTTGAGG 
     3361 ACACCGAAGC CCTTGTGAAT GCAGCAACAA AGCTCCAGCA TCCAGTCGCA AGAGGTCTGC 
     3421 CCTCCAGTCA GGAGCCTCTC TTGCAGGTGC CTGAGAAGCC CTCCCAGAAG GAGCTGGAGG 
     3481 CCATGGCCTT GGGCACCCCA GCAGAAGCAA TTGAAATCAC AGCTCCCGAG GGTGCCTTTG 
     3541 CTTCTGCAGA TACCCTCCTC AGCAGGCTGG CCCATCCCGC CTCTCTCTGT GGTGCACTTG 
     3601 GCTATCTGGA GCCTGACTTA GCAGAAAAGA ACCCCCCAGT ATTTGCCCAG AAACTTCAGG 
     3661 AGGAGTTAGA GTTTGCTGTC ATGCGGATAG AAGCCCTGAA GCTGGCCAGG CAGATCGCCC 
     3721 TGGCTTCCCG CAGCCGCCAG GACACCAAGA GGGAAGCTGC TCACCCACCA GATGTCTCCA 
     3781 TCTCCAAAAC TGCCTTGTAC TCCCGCATCG GGTCCACCGA GGTGGAGAAA CCCCCAGGCC 
     3841 TTCTGTTCCA GCAGCCAGAC CTGGACTCTG CACTCCAGGT TGCCAGAGCA GAGGTCATCG 
     3901 CCAAGGAGAG AGAAGTCTCA GAGTGGAGGG ATAAATATGA AGAAAGCCGA CGGGAAGTGG 
     3961 TGGAAATGAG GAAAATTGTG GCCGAATATG AGAAGACCAT TGCACAGATG ATCGAGGACG 
     4021 AACAGAGAGA AAAATCCATC TCCCACCAAA CTGTACAGCA GCTGGTCCTG GAGAAGGAGC 
     4081 AAGCCCTGGC TGACCTGAAC TCTGTGGAAA AGTCTCTGGC TGACCTCTTC AGGAGATACG 
     4141 AAAAGATGAA GGAGGTCCTG GAAGGTTTTC GAAAGAATGA AGAGGTGTTG AAGAAATGTG 
     4201 CGCAGGAGTA CCTATCCCGA GTGAAGAAAG AGGAACAGAG GTACCAGGCC CTGAAGGTGC 
     4261 ACGCCGAAGA GAAACTGGAC AGAGCCAATG CAGAGATTGC CCAGGTTCGC GGCAAGGCCC 
     4321 AGCAAGAGCA AGCAGCCTAC CAGGCTAGCC TGCGGAAGGA GCAGCTTCGA GTGGATGCTC 
     4381 TGGAAAGAAC GCTGGAGCAG AAGAATAAAG AGATAGAAGA ACTCACCAAG ATTTGTGACG 
     4441 AGCTGATCGC CAAGATGGGA AAAAGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//