LOCUS       MR221059                8157 bp    DNA     circular  SYN 
DEFINITION  Lifr (Myc-DDK-tagged) - Mouse leukemia inhibitory factor receptor (Lifr), transcript variant 1
ACCESSION   NM_013584
VERSION     NM_013584.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4314..4405 
                     /label="Myc-DDK"  
     misc_feature    4435..5020 
                     /label="PolyA signal"  
     misc_feature    4492..4511 
                     /label="Restriction Enzyme"  
     misc_feature    5296..5968 
                     /label="ColE1"  
     misc_feature    6398..7192 
                     /label="Kan/Neo"  
     misc_feature    7227..7529 
                     /label="SV40 ori"  
     misc_feature    7615..8092 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4296..4302 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4296 
                     /label="Lifr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCTTAC TCATGGTGGA GACAGCCATC GTGGATGGTA GACAATAAAA 
     1081 GATCGAGGAT GACTCCAAAC CTGCCATGGC TCCTGTCAGC TCTGACCCTC CTGCATCTGA 
     1141 CGATGCATGC AAACGGTCTG AAGAGAGGGG TACAAGACTT GAAATGCACA ACCAACAACA 
     1201 TGCGAGTGTG GGACTGCACG TGGCCAGCTC CCCTCGGGGT CAGCCCTGGA ACTGTTAAAG 
     1261 ATATTTGCAT TAAAGACAGG TTCCATTCTT GTCACCCATT AGAGACAACA AACGTTAAAA 
     1321 TTCCAGCTCT TTCACCTGGT GATCACGAAG TCACAATAAA TTATCTAAAT GGCTTTCAGA 
     1381 GTAAATTCAC GTTGAATGAA AAAGATGTCT CTTTAATTCC AGAGACTCCC GAGATCCTGG 
     1441 ATTTGTCTGC TGACTTCTTC ACCTCCTCCT TACTACTGAA GTGGAACGAC AGAGGGTCTG 
     1501 CTCTGCCTCA CCCCTCCAAT GCCACCTGGG AGATTAAGGT TCTACAGAAT CCAAGGACGG 
     1561 AACCAGTAGC ACTCGTGTTA CTCAACACAA TGCTGAGTGG TAAAGATACC GTTCAGCACT 
     1621 GGAACTGGAC CTCAGACCTG CCCTTGCAAT GTGCCACTCA CTCGGTGAGC ATTCGATGGC 
     1681 ACATTGACTC GCCTCATTTC TCCGGTTACA AAGAGTGGAG TGACTGGAGC CCGCTGAAGA 
     1741 ACATCTCCTG GATTCGTAAT ACAGAGACTA ATGTTTTTCC TCAAGACAAA GTGGTGCTCG 
     1801 CAGGCTCAAA CATGACAATT TGTTGTATGA GTCCAACGAA AGTGCTTTCA GGACAGATCG 
     1861 GCAATACCCT TCGTCCTCTC ATCCATCTGT ACGGGCAAAC CGTTGCGATC CATATCCTGA 
     1921 ACATCCCCGT TTCTGAAAAC AGTGGCACAA ACATCATTTT CATCACAGAC GACGATGTGT 
     1981 ACGGAACGGT GGTCTTTGCA GGCTATCCTC CCGATGTTCC TCAGAAGCTG AGCTGTGAGA 
     2041 CACATGACTT AAAAGAGATT ATATGTAGCT GGAATCCAGG AAGGATAACT GGACTGGTGG 
     2101 GCCCACGAAA TACAGAATAC ACCCTGTTTG AAAGCATTTC AGGAAAATCG GCAGTATTTC 
     2161 ACAGGATTGA AGGACTTACA AACGAGACCT ACCGGTTAGG CGTGCAAATG CATCCCGGCC 
     2221 AAGAAATCCA TAACTTCACC CTGACTGGTC GCAATCCACT GGGGCAGGCA CAGTCAGCAG 
     2281 TGGTCATCAA TGTGACTGAG AGAGTTGCTC CTCATGATCC GACTTCGTTG AAAGTGAAGG 
     2341 ACATCAATTC AACAGTTGTT ACATTTTCTT GGTATTTACC AGGAAATTTT ACAAAGATTA 
     2401 ATCTTTTATG TCAAATTGAA ATTTGTAAAG CTAATTCCAA GAAAGAAGTG AGGAATGCCA 
     2461 CAATCAGAGG AGCCGAGGAT TCAACTTACC ATGTTGCTGT AGACAAATTA AATCCATACA 
     2521 CTGCATACAC TTTCCGGGTT CGTTGTTCTT CCAAGACTTT CTGGAAGTGG AGCAGGTGGA 
     2581 GTGATGAGAA GCGACATCTA ACCACAGAAG CCACTCCTTC AAAGGGACCA GACACTTGGA 
     2641 GAGAGTGGAG TTCTGATGGA AAAAATCTAA TCGTCTACTG GAAGCCTTTA CCTATTAATG 
     2701 AAGCTAATGG AAAAATACTT TCCTACAATG TTTCGTGTTC ATTGAACGAG GAGACACAGT 
     2761 CAGTTTTGGA GATCTTCGAT CCTCAACACA GAGCAGAGAT ACAGCTTAGT AAAAATGACT 
     2821 ACATCATCAG TGTGGTGGCA AGAAATTCTG CTGGCTCATC ACCACCTTCG AAAATAGCTA 
     2881 GTATGGAAAT CCCAAATGAC GACATCACAG TAGAGCAAGC GGTGGGGCTA GGAAACAGGA 
     2941 TCTTCCTCAC CTGGCGTCAC GACCCCAACA TGACTTGTGA CTACGTAATT AAATGGTGCA 
     3001 ACTCATCTCG GTCTGAGCCC TGCCTCCTGG ACTGGAGAAA GGTTCCTTCA AACAGCACGG 
     3061 AGACTGTCAT AGAGTCTGAT CAGTTTCAGC CAGGAGTAAG ATACAACTTT TACCTCTATG 
     3121 GGTGCACTAA CCAGGGATAC CAACTGTTAC GTTCCATAAT TGGATACGTA GAAGAACTGG 
     3181 CTCCCATTGT CGCGCCAAAT TTCACCGTGG AAGATACGTC GGCAGACTCG ATATTAGTGA 
     3241 AATGGGACGA CATCCCCGTG GAAGAGCTCC GAGGCTTCCT AAGAGGGTAT TTATTTTACT 
     3301 TTCAGAAAGG AGAGAGAGAT ACGCCCAAGA CGAGGAGCTT GGAGCCACAT CATTCTGACA 
     3361 TCAAGCTAAA GAACATCACT GACATATCCC AGAAGACACT GAGGATTGCT GACCTTCAGG 
     3421 GTAAAACCAG TTACCATCTG GTCCTGCGAG CCTATACACA TGGTGGTCTG GGCCCAGAGA 
     3481 AGAGCATGTT TGTGGTGACC AAGGAAAACT CTGTGGGATT GATTATTGCC ATCCTCATCC 
     3541 CAGTGGCTGT GGCTGTCATT GTTGGCGTGG TAACGAGCAT CCTTTGCTAT CGGAAGCGAG 
     3601 AATGGATTAA GGAAACATTC TACCCAGATA TTCCCAATCC AGAAAACTGT AAGGCGCTAC 
     3661 AGTTTCAGAA GAGCGTCTGC GAGGGAAGCA ATGCTCTTAA GACATTGGAA ATGAACCCCT 
     3721 GTACCCCGAA CAACGTTGAA GTCCTGGAAT CTCGGTCGAT AGTCCCTAAA ATAGAAGATA 
     3781 CAGAAATAAT TTCCCCAGTT GCTGAGCGTC CTGGGGAAAG ATCTGAGGTG GACCCTGAGA 
     3841 ACCATGTGGT CGTGTCTTAC TGCCCACCCA TCATCGAGGA GGAAATAACG AACCCCGCGG 
     3901 CCGATGAAGT GGGAGGGGCT TCCCAGGTCG TGTACATCGA TGTGCAGTCC ATGTATCAGC 
     3961 CGCAAGCCAA AGCAGAGGAA GAGCAGGACG TGGATCCTGT GGTGGTGGCA GGCTATAAGC 
     4021 CACAGATGCG CCTTCCCATC AGCCCCGCTG TGGAAGACAC AGCAGCAGAA GATGAAGAGG 
     4081 GTAAGACCGC CGGTTACAGA CCTCAGGCCA ATGTAAACAC TTGGAATTTG GTCTCTCCAG 
     4141 ATTCCCCGAG GTCCACAGAC AGCAACAACG AAGTTGTCTC TTTTGGAAGT CCGTGCTCCA 
     4201 TCAATTCCAG GCAATTCTTG ATTCCTCCTA AAGACGAAGA CTCTCCTAAA TCTAATGGAG 
     4261 GAGGGTGGTC CTTTACAAAC TTCTTCCAGA ACAAACCAAA TGACACGCGT ACGCGGCCGC 
     4321 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4381 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4441 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4501 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4561 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4621 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4681 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4741 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4801 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4861 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4921 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4981 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5041 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5101 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5161 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5401 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5641 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5881 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5941 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6001 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6061 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6121 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6181 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6241 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6301 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6361 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6421 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6481 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6541 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6601 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6661 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6721 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6781 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6841 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6901 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6961 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7021 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7081 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7141 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7201 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7261 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7321 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7381 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7561 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7621 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7681 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7741 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7801 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7861 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7921 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7981 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8041 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8101 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//