LOCUS       MR221047                8448 bp    DNA     circular  SYN 
DEFINITION  Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 2
ACCESSION   NM_001160316
VERSION     NM_001160316.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4605..4696 
                     /label="Myc-DDK"  
     misc_feature    4726..5311 
                     /label="PolyA signal"  
     misc_feature    4783..4802 
                     /label="Restriction Enzyme"  
     misc_feature    5587..6259 
                     /label="ColE1"  
     misc_feature    6689..7483 
                     /label="Kan/Neo"  
     misc_feature    7518..7820 
                     /label="SV40 ori"  
     misc_feature    7906..8383 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4587..4593 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4587 
                     /label="Nfasc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGCAG CAGGCGCCAC CCTGGGTCCA CATAGCCCTC ATCCTCTTCC 
     1081 TCCTCAGCCT CGGAGGGGCC ATCGAGATTC CGATGGACCT GACCCAACCC CCAACTATCA 
     1141 CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG ATTGAATGTG 
     1201 AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA TTCTTCAACA 
     1261 TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG ATCGACTTCC 
     1321 GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC CGGAACAAAT 
     1381 TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC CTGTGGCCCA 
     1441 AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA CAGTGCAACC 
     1501 CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG GAGCCCATCA 
     1561 CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC AACGTCATGC 
     1621 TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC CACACCATTC 
     1681 AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT GACTCGTCCT 
     1741 TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG CCCAGCTTCA 
     1801 TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC CTGCTGCTGG 
     1861 AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA GGTGGGGACC 
     1921 TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC ACCAATGTCT 
     1981 CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC AGCATCCGGC 
     2041 ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC AAGAACCTGA 
     2101 TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC CCGAAGCCGA 
     2161 CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT CCCAACCGTG 
     2221 AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG GCAGTGTACC 
     2281 AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC AGCGTGTTAG 
     2341 ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC CTTTATAACC 
     2401 GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA TGGTTTAAGA 
     2461 ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC GGCAGTCTAG 
     2521 AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC ACCAACATCC 
     2581 TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG ATCTACAGGA 
     2641 TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC CGCGTGAAAC 
     2701 ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA CTCTACATTG 
     2761 GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA GAGCGGGACC 
     2821 AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA AAGGCCTACC 
     2881 TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA CCCAAAGGGC 
     2941 GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT GTGAGGCTGA 
     3001 CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT CAGTTTGAAG 
     3061 AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC AGCGTCAACT 
     3121 CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC GCTGTCAACG 
     3181 AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT GGGGCACCCC 
     3241 CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT ATGGAGATCA 
     3301 CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC ATTGTCAAGT 
     3361 GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC TCTCGCTACG 
     3421 TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT GAAAATGACT 
     3481 TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT TATCCCAGGG 
     3541 CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC CTTCAGTGGA 
     3601 ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT TACTACTGGA 
     3661 GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG GCCAGCTTCC 
     3721 CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC TACAAGCTGG 
     3781 AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG GAATTCACCA 
     3841 CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC AACCTGGAGA 
     3901 CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT GGATACATCC 
     3961 TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA AACTTCTCTC 
     4021 CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC CGCTTCTCCC 
     4081 TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC CCAGCACCTC 
     4141 CAAATGAAGC TACTCCAACT GCAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG 
     4201 GCTGGTTCAT CGGGCTCATG TGTGCCATTG CCCTTCTGGT GCTGATCCTT CTCATCGTCT 
     4261 GCTTCATCAA GAGGAGTCGA GGTGGCAAGT ACCCAGTGCG GGAAAAGAAG GATGTCCCCT 
     4321 TGGGTCCTGA AGACCCCAAA GAAGAAGATG GCTCATTTGA CTACAGTGAT GAGGACAACA 
     4381 AGCCCCTGCA GGGCAGCCAG ACATCTCTGG ATGGCACCAT CAAGCAGCAG GAGAGCGATG 
     4441 ACAGCCTGGT GGACTATGGC GAAGGCGGCG AGGGCCAGTT CAATGAAGAT GGCTCCTTTA 
     4501 TTGGCCAGTA CACTGTCAAA AAGGACAAGG AGGAAACGGA GGGCAATGAG AGCTCAGAGG 
     4561 CCACATCACC AGTCAATGCC ATCTATTCCC TTGCCACGCG TACGCGGCCG CTCGAGCAGA 
     4621 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4681 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4741 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4801 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4861 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4921 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4981 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5041 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5101 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5161 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5221 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5281 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5341 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5401 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5461 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5521 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5581 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5641 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5701 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5761 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5821 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5881 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5941 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6001 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6061 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6121 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6181 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6241 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6301 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6361 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6421 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6481 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6541 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6601 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6661 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6721 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6781 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6841 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6901 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6961 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7021 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7081 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7141 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7201 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7261 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7321 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7381 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7441 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7501 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7561 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7621 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7681 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7801 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7861 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7921 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7981 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8041 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8101 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8161 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8221 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8281 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8341 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8401 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//