LOCUS       MR220981                7542 bp    DNA     circular  SYN 
DEFINITION  Kif20a (Myc-DDK-tagged) - Mouse kinesin family member 20A (Kif20a), transcript variant 3
ACCESSION   NM_009004
VERSION     NM_009004.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3699..3790 
                     /label="Myc-DDK"  
     misc_feature    3820..4405 
                     /label="PolyA signal"  
     misc_feature    3877..3896 
                     /label="Restriction Enzyme"  
     misc_feature    4681..5353 
                     /label="ColE1"  
     misc_feature    5783..6577 
                     /label="Kan/Neo"  
     misc_feature    6612..6914 
                     /label="SV40 ori"  
     misc_feature    7000..7477 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3681..3687 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3681 
                     /label="Kif20a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCACCGG ATCCTTTCTC CGCCAGCGGG CTTACTCTCT GATGAGGATG 
     1081 TTGTAGACTC TCCCATATTA GAATCCACAG CTGCAGATCT GAGATCTGTT GTCCGAAAGG 
     1141 ACCTGTTGTC AGACTGCTCT GTCATCTCTG CCTCTCTGGA GGACAAGCAG GCTCTACTGG 
     1201 AGGACACCTC AGAGAAGGTG AAAGTTTACC TTCGAATTCG ACCATTCTTG ACTTCAGAGT 
     1261 TGGACCGACA GGAAGATCAG GGCTGCGTCT GCATTGAGAA TACAGAAACC CTTGTGCTGC 
     1321 AGGCACCCAA AGACTCCTTT GCCTTGAAGA GTAATGAGAG GGGAGTTGGT CAGGCCACTC 
     1381 ACAAATTTAC CTTTTCCCAG ATATTTGGGC CAGAAGTGGG ACAGGTGGCC TTCTTCAATC 
     1441 TGACCATGAA GGAGATGGTG AAGGATGTGC TCAAAGGGCA GAACTGGCTC ATCTACACCT 
     1501 ATGGAGTCAC CAACTCGGGG AAAACGTACA CCATTCAAGG TACTAGTAAG GATGCGGGGA 
     1561 TCCTGCCCCA GTCCCTGGCT CTCATCTTTA ATAGTCTCCA GGGCCAGCTT CATCCGACAC 
     1621 CTGACCTGAA GCCCTTATTG TCCAATGAAG TAATCTGGCT AGACAGCAAG CAGATTCGAC 
     1681 AGGAGGAAAT GAAGAAGCTG TCCTTGCTAA TTGGAGGCCT CCAAGAGGAG GAGCTGTCCA 
     1741 CCTCCGTGAA GAAACGTGTC CACACTGAAA GTCGGATAGG TGCCAGCAAC AGCTTTGACA 
     1801 GTGGCGTTGC TGGGCTGTCT TCCACCAGTC AGTTCACCAG CAGCAGCCAG CTAGATGAAA 
     1861 CAAGTCAATT ATGGGCACAA CCAGACACTG TCCCAGTAAG TGTTCCAGCA GACATTCGCT 
     1921 TCTCTGTCTG GATCTCCTTC TTTGAGATCT ACAATGAATT GCTTTATGAC CTGTTAGAAC 
     1981 CACCTAGCCA TCAGCATAAG AGACAGACAC TTCGGCTGTG TGAGGATCAG AACGGCAATC 
     2041 CTTACGTGAA AGATCTCAAT TGGATTCATG TTCGTGATGT TGAGGAAGCC TGGAAACTAT 
     2101 TGAAAGTGGG TCGCAAGAAC CAGAGCTTTG CCAGCACCCA CATGAACCAG CAATCCAGCC 
     2161 GCAGTCACAG CATCTTCTCA ATCAGAATCT TGCACCTTCA GGGAGAAGGG GATATAGTGC 
     2221 CCAAGATCAG TGAACTGTCA CTCTGTGACC TGGCTGGCTC TGAGCGCTGC AAACATCAAA 
     2281 AAAGTGGTGA GCGGCTAAAG GAGGCAGGGA ACATTAACAC TTCTCTGCAC ACCCTGGGCC 
     2341 GCTGTATTGC TGCCCTGCGA CAGAATCAGC AGAACCGGTC AAAGCAGAAC CTGATTCCTT 
     2401 TCCGTGACAG CAAGTTGACT CGTGTGTTCC AAGGCTTCTT CACAGGTCGA GGTCGTTCCT 
     2461 GTATGATTGT CAATGTGAAT CCCTGTGCAT CTACATATGA CGAGACTCTT CATGCAGCCA 
     2521 AATTCTCAGC CCTAGCCAGC CAGCTTGTGC ACGCCCCACC TGTGCATCTG GGAATCCCAT 
     2581 CCCTGCATTC ATTCATCAAG AAGCACAGTC CTCAGGTTGG CCCTGGCTTA GAGAAAGAGG 
     2641 ACAAGGCCGA CTCAGACCTT GAGGACAGTC CTGAAGACGA AGCTGACGTC TCCGTGTATG 
     2701 GCAAGGAGGA ACTACTCCAG GTGGTGGAAG CCATGAAAGC GCTACTTTTA AAAGAACGAC 
     2761 AAGAAAAGCT GCAGTTGGAG ATACAGCTCC GAGAGGAAAT TTGCAACGAA ATGGTGGAAC 
     2821 AGATGCAACA GCGGGAGCAG TGGTGCAGTG AGCGTTTGGA CAACCAAAAG GAACTGATGG 
     2881 AGGAACTGTA CGAGGAGAAA CTGAAGATCC TGAAGGAATC ACTGACAACT TTCTACCAAG 
     2941 AGCAGATCCA GGAGCGGGAT GAAAAAATTG AAGAGCTAGA AACTCTGTTG CAGGAAGCCA 
     3001 AACAGCAGCC AGCGGCACAA CAGTCAGGGG GGCTGTCCCT CCTCCGGCGG TCACAGAGGC 
     3061 TGGCAGCGTC TGCCTCCACT CAGCAGTTCC AGGAAGTTAA AGCTGAACTA GAGCAATGCA 
     3121 AAACAGAGTT AAGCTCTACC ACTGCAGAGC TGCACAAGTA TCAGCAAGTG TTAAAACCGC 
     3181 CACCCCCAGC CAAGCCTTTC ACCATTGATG TGGATAAGAA GTTAGAGGAG GGCCAGAAGA 
     3241 ATATAAGACT GCTACGGACA GAGCTCCAGA AACTAGGACA GTCTCTGCAG TCAGCAGAAA 
     3301 GAGCCTGTTG CCACAGCACC GGAGCAGGAA AACTTCGTCA AGCATTAACC AACTGTGATG 
     3361 ACATCTTAAT CAAGCAGAAC CAGACCCTGG CTGAGCTGCA GAATAACATG GTGCTAGTGA 
     3421 AACTGGACCT TCAGAAAAAG GCAGCCTGCA TTGCTGAGCA GTATCACACT GTGCTAAAGC 
     3481 TCCAAGGCCA AGCCTCTGCC AAAAAGCGGC TGGGAGCAAA CCAGGAAAAC CAGCAACCAA 
     3541 ATCATCAACC CCCAGGAAAG AAACCATTCC TGCGAAACTT ACTTCCCCGA ACACCTACCT 
     3601 GCCAAAGCTC AACAGACAGC AGCCCTTATG CGCGGATCTT GCGCTCACGG CACTCTCCTT 
     3661 TACTCAAATC TCCCTTTGGC AAAAAATACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3721 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3781 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3841 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3901 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3961 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4021 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4081 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4141 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4201 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4261 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4321 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4381 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4441 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4501 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4561 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4621 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4681 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4741 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4801 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4861 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4921 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4981 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5041 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5101 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5161 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5221 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5281 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5341 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5401 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5461 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5521 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5581 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5641 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5701 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5761 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5821 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5881 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5941 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6001 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6061 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6121 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6181 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6241 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6301 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6361 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6421 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6481 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6541 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6601 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6661 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6721 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6781 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6841 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6961 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7021 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7081 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7141 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7201 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7261 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7321 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7381 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7441 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7501 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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