LOCUS       MR220884                8625 bp    DNA     circular  SYN 
DEFINITION  Smc1b (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1B (Smc1b)
ACCESSION   NM_080470
VERSION     NM_080470.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="Smc1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCACCTT GAGCTGCTGC TCGTGGAGAA TTTCAAGTCG TGGCGAGGCC 
     1081 GCCAGGTCAT CGGCCCTTTC AAGAGGTTCA CCTGCATCAT TGGCCCCAAC GGCTCCGGAA 
     1141 AATCTAATGT AATGGATGCA CTTAGTTTTG TAATGGGAGA AAAGACAACT AATTTAAGAG 
     1201 TAAAAAACAT TCAAGAACTT ATTCATGGAG CACATACTGG AAAACCTGTT TCTTCTTCTG 
     1261 CAAGTGTGAC AATTATATAC ATAGAGGACA GTGGAGAAGA GAAAACATTC ACAAGGATTA 
     1321 TCCGAGGGGG GTGCTCAGAA TATCATTTTG GGGATAAACC CGTGAGTCGT TCTGTGTATG 
     1381 TAGCCCAGTT GGAAAACATA GGCATAATAG TCAAAGCACA GAACTGTCTA GTTTTTCAGG 
     1441 GAACTGTAGA GTCAATATCC ATGAAAAAGC CCAAAGAGAG AACCCAGTTT TTTGAAGAAA 
     1501 TCAGTACTTC AGGAGAATTT ATAGGAGAAT ATGAGGCAAA GAAAAAGAAA TTACAAAAAG 
     1561 CTGAAGAAGA TGCACAATTT CATTTTAATG TGAAAAAAAA TGTAGCTGCA GAACGGAAGC 
     1621 ATGCAAAAAT AGAGAAAGAA GAGGCAGAAC ATTACCAAAA TCTCCTTGAA GAATTGAAAA 
     1681 TAAACAAGAT ACAACTGATG CTTTTCCAAC TATATTATAA TGAGGAAAAA ATCAATGTTT 
     1741 TAAACACTGA ATTAGAGCAA ATGGACGGGA ATTTAAGTGT TGTAAAAGAC ACTCTTTCTC 
     1801 ACCATGAAAA CATATTTAAA GCTAAGAAAA AGGATTATGG AATGTTAACT AGACAACTAC 
     1861 AGCAAACAGC AAAAGAACTG AAATCTGTTG AAGCAATTTT AAATCAGAAG AGGCCTCAGT 
     1921 ACATTAAGGC TAAAGAAAAC ACTTCTCATC ATCTAAAAAA ATTAGATTTG TCTAAGAAAT 
     1981 TGATAACAGA CAATGAAAAA CAATGTTCTA AGCAGGAAGA TGGCATACGA GCCTTAGTGG 
     2041 CAGAACTGGC TGATTTGGAT AGAGCATGGA AAAGTTTTGA AAAGCAGATG GAAGAGAAAA 
     2101 TCTTACAAAA AGGGCGAGAT ATTGAATTGG AAAATAGCCA GCTGGATCGT TACAAACTGC 
     2161 TTAAGGAGCA AGTCAGACGG AAGGTTGGTA TAATGACACA ACAACTGGAA AAACTGCAGT 
     2221 GGGAACAGAA GGCAGAAAAA GAAAGACTTG CATTTGAAAA GAGGAGGCAT GGAGACACTC 
     2281 AGGGAAATCT AAAACAGATA AAAGAACAAA TAGAAGAGCA TAAAAAACGA ATAGAGAAGT 
     2341 TGGAGGAATA TACAAAGACG TGCATGGATT GCTTGGAAGA TAAAAAACAG CAAGAAGAGG 
     2401 CCCTGAAAAA AGAAATTGAA AATACGAAAT CAAGAATGTC TGAAGTTAAT GAAGAATTGA 
     2461 GTCTTATTAG AAATGAATTG CAGAATGCTG GAATTGATAA CCATGAGGGA AAACGTCAGC 
     2521 AGAAAAGAGC AGAAGTTCTG GAACACCTTA AAAGACTTTA CCCAGATTCT GTGTTTGGAA 
     2581 GACTGCTTGA TCTGTGTCAT CCTATTCATA AGAAGTACCA GCTGGCTGTG ACTAAGCTTT 
     2641 TTGGCCGGTA CATGGTTGCC ATTGTTGTAG CCTCAGAAAA GATAGCGAAA GATTGTATTC 
     2701 GATTTCTGAA GGCAGAAAGA GCTGAACCTG AGACATTCCT TGCTCTAGAT TACCTTGATA 
     2761 TCAAGCCAAT CAATGAACGA CTAAGGGAAA TTAAAGGCTG TAAGATGATG ATCGATGTTA 
     2821 TAAAGACCCA GTTTCCTCAG CTGAAGAAAG TGATTCAGTT TGTTTGTGGA AATGGCCTTG 
     2881 TCTGTGAGAC TGTGGAAGAA GCAAGACATA TTGCATTCGG TGGACCTGAA AGACGGAAGG 
     2941 CAGTAGCACT TGATGGAACA CTGTTTTTGA AATCTGGAGT GATTTCTGGA GGGTCAAGTG 
     3001 ACTTAAAGCA CAAAGCTCTG TGCTGGGATG AGAAAGAGTT ACACAATCTA AGAGACAAAA 
     3061 GAAGCCAACT AGTCCAAGAG CTAAAGGAGT TAATGAAGAC ACTCCGCAAG GAAACAGATC 
     3121 TGAAGCAAAT ACAGACTTTA GTACAAGGAA CCAATACACG ACTCAAATAT TCACAAAATG 
     3181 AACTAGAGAT GATTAAAAAG AAGCACCTTG CTACATTTTA CCGGGAACAA TCTCAGCTAC 
     3241 AAAGTGAATT ACTGAATATT GATTCTCAAT GTACTATGTT GAGTGAAGGA ATCAACAAAC 
     3301 AGCAACAAAA AATTGAAGAA TTTCAAGATA AGATAGATGA GGTAGAAGAT GACATATTCC 
     3361 AAGACTTCTG TGAAGAAATT GGTGTGGAAA ATATCCGTGA ATTTGAGAAT AAACATGTTA 
     3421 AACAGCAGCA AGAAAATGAT CAAAAAAGAT TAGAGTTTGA AAAACAAAAA ACTCGGCTTA 
     3481 ATATTCAGCT CGAATATAGT CGAAATCAGC TTAAGAAGAA ACTGAATAAT ATCGACACAT 
     3541 TAAAAACCAC AATCCAGAAG GGCAAAGAAG ATATTGATAA CCTAAAAAAG ACCGAAGAAG 
     3601 AATGTCTGAA AATTGTTGAG GAACTCATGG TGAAGCAAGA GCAAATTAAG GAAGTGCTTG 
     3661 CCACACAGAG TTCCAACATT GAAAAAATTC ACATACAAAT TGAAGAGGAA CGCAAGAAGG 
     3721 TTTTGGCTGT TGATAGGGAA GTTGGAAAAT TACAGAAGGA AGTTGTAATC ATTCAGGGTT 
     3781 CTTTGGAACA GAAACTGCTA GAGAAACATA ACTTGCTGCT AGATTGCAAA GTTCAAGACA 
     3841 TTGACATAAG TCTTGTGCTG GGGTCATTGG AGGACATCAT TGAAATGGAG CTAACTGAAA 
     3901 CAGAAAGCAC CCAGGCAACA GCTGATATCT ATGAGAAAGA AGCATCCATC CAAATAGACT 
     3961 ACAGCCCTCT AAGGGAGGAT TTAAAGGCTC TACAATCAGA TAAGGAGGTG GAGGCCCACC 
     4021 TTACACTCCT TCTACAGCAA GTAGCATCCC AAGAAAACAC TCTACTGAAG ACTACAGCTC 
     4081 CAAACTTGCG AGCACAGGAG AACTTAAAGA CTGTCAGAGA CAAGTTTCAA GAATCTGCAG 
     4141 ATGTTTTTGA GGCCAGCAGA AAGGAAGCCA GAATATGTAG GCAAGAGTTT GAACAGGTGA 
     4201 AAAGACGGAG GTACGATGCT TTCAGTCAAT GTTTTGAACA CATCTCAGTC TCAATTGATC 
     4261 AAATCTACAA GAAGCTCTGC AGGAACAACA GTGCCCAGGC ATTTCTTAGC CCAGAGAACC 
     4321 CTGAAGAACC TTACTTAGAT GGAATTAGCT ACAACTGTGT GGCTCCAGGC AAACGGTTCA 
     4381 TGCCCATGGA CAACCTGTCA GGGGGAGAAA AGTGTGTGGC TGCTCTGGCT CTTCTGTTTG 
     4441 CTGTACACAG TTTTCGGCCT GCTCCATTCT TTGTATTAGA TGAAGTAGAT GCAGCCCTGG 
     4501 ACAATACTAA CATTGGCAAA GTCTCAAGTT ACATCAAAGA GCAGAGTCAG GAACAGTTTC 
     4561 AGATGATAAT CATTTCCCTG AAAGAGGAGT TCTACTCCAA AGCTGATGCA CTGATAGGCG 
     4621 TCTACCCAGA GCACAATGAG TGCATGTTCA GCCATGTGTT GACTCTGGAC CTTTCCAAGT 
     4681 ATCCAGACAC CGAAGACCAA GAAGGCAGCA GGAGCCACCG GAAGCCCAGA GTACCACGAG 
     4741 TATCAATGTC TCCAAAGTCT CCCCAGTCTC GTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//