LOCUS       MR220816                8250 bp    DNA     circular  SYN 
DEFINITION  Dgkz (Myc-DDK-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1
ACCESSION   NM_001166597
VERSION     NM_001166597.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4407..4498 
                     /label="Myc-DDK"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    4585..4604 
                     /label="Restriction Enzyme"  
     misc_feature    5389..6061 
                     /label="ColE1"  
     misc_feature    6491..7285 
                     /label="Kan/Neo"  
     misc_feature    7320..7622 
                     /label="SV40 ori"  
     misc_feature    7708..8185 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4395 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="Dgkz"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGACCTTC TTTAGGAAGC ATTTCCAACG AAAGGCACGA GGTCCCGGAG 
     1081 AGAGGCAGCG GCGGTCCAGT GGCGTGGGGC TGCCCACAGG CAAGGCTCGA CGCCGCTCCC 
     1141 CTGCAGGGCA GGCGTCCTCT TCACTGGCCC AGAGGCGCCG CTCCAGTGCA CAGCTCCAGG 
     1201 GCTGTTTTCC CAGCTGCGGG GTGGGCACGA GAGGTGCCAG CCGCAGGCGA TCCAGCACTG 
     1261 CACCCCCTAC CTGCAATCCC CGCTTCATGG TGGATGTGGT GCCTACCCTA CAGCCTGTCA 
     1321 CAGTTGGGGC CCAGCTTCTG GGTGCACCCC TGCTGTTGGC TGGGCTCACA AGCATGAAGG 
     1381 AGGAAGAGGA GGAGGAAGGG GTCCAGGAGG GAAATGAGAC AGCTGAGGCG ACAAGTGATG 
     1441 CTAGGCCAGG TACCCGGACA ACGCCCTTCT CACACCAGGG CACCTGGCCA CTGCTTCCCA 
     1501 TGCCCCGCTG CCTGCGTCGA GCCTCCTCCC ACCTGCTCCC TGCTGATGTG GAGTGTGGCC 
     1561 ATACCCTTTG GGGCCTGCAT GGTCACTATC GACGCCTCAG CCAGCGGTGG CCCTCAGGCC 
     1621 AGCACCCTGG TCTTGGGGGC CGAAGCCCAA GAGCCTCGGG CGCTGCAGCA TGCCCCATGA 
     1681 TACCGGCCCG TGTGCGCCCA CTGTCTCGCA GACGCCAGGT AGCTCTAAGG CGGAAGTCAG 
     1741 CTGGACCCCA GCCCTGGAGC ACCCTGCTTA TGAAAGCCAT CACCAAGTCG GGCCTCCAGC 
     1801 ACCTGGCACC CCCTCCTCCC ACGCCCGGGG CCCCGTGCGG TGAATCTGAG GAGCAGATCC 
     1861 AGAGCACTGT GGACTGGAGT GAGTCAGCAG TGTATGGGGA GCACATCTGG TTTGAGACCA 
     1921 ACGTGTCCGG TGACTTCTGC TATGTCGGGG AGCAGCACTG TGTAGCTAAG ATGCTGCCGA 
     1981 AGTCAGCGCC CAGAAAAAAG TGTGCAGCCT GTAAGATCGT GGTGCATACC CAATGCATTA 
     2041 AGCAGCTGGA AAAGATCAAT TTCCGCTGTA AGCCGTCCTT CCGCGAATCA GGCTCCAGGA 
     2101 ATGTCCGTGA GCCAACCTTC GTAAGACACC ACTGGGTCCA CAGACGACGC CAGGATGGCA 
     2161 AGTGTCGGCA CTGTGGGAAG GGCTTCCAGC AGAAGTTCAC CTTCCACAGC AAGGAGATCG 
     2221 TAGCCATCAG CTGCTCCTGG TGCAAACAGG CATACCACAG CAAGGTGTCG TGCTTCATGA 
     2281 TGCAACAGAT TGAGGAACCC TGCTCCCTAG GGGTGCATGC AGCCGTGGTC ATCCCACCCA 
     2341 CCTGGATCCT GCGGGCCCGG AGGCCCCAGA ACACCCTCAA GGCCAGCAAG AAGAAAAAGA 
     2401 GAGCGTCCTT CAAGAGGAGG TCCAGCAAGA AAGGACCTGA GGAAGGCCGC TGGAGACCCT 
     2461 TCATCATCAG ACCCACCCCA TCCCCCCTCA TGAAACCCCT GCTGGTGTTT GTGAACCCCA 
     2521 AGAGTGGGGG CAACCAGGGT GCCAAGATCA TCCAGTCTTT TTTGTGGTAT CTGAATCCCC 
     2581 GACAAGTCTT TGATCTGAGC CAGGGAGGAC CCAGGGAAGC GCTGGAAATG TACCGCAAAG 
     2641 TACATAATTT GAGGATTCTG GCTTGCGGGG GTGATGGCAC GGTTGGCTGG ATTCTCTCCA 
     2701 CCCTGGACCA GCTGCGCTTA AAACCACCGC CTCCTGTAGC CATCCTGCCT CTGGGCACTG 
     2761 GCAATGACCT GGCCCGAACC CTCAACTGGG GTGGGGGTTA CACAGATGAG CCTGTGTCAA 
     2821 AGATCCTTTC CCATGTTGAG GAGGGGAATG TGGTACAGCT GGACCGTTGG GACCTCCGAG 
     2881 CAGAGCCCAA CCCTGAGGCG GGGCCTGAGG AGCGAGATGA TGGAGCCACT GACCGGCTGC 
     2941 CCCTGGATGT CTTTAACAAC TACTTCAGCC TGGGCTTCGA TGCCCACGTC ACCCTGGAAT 
     3001 TTCATGAGTC TCGAGAAGCC AACCCAGAGA AGTTCAACAG CCGCTTTCGG AATAAGATGT 
     3061 TCTATGCTGG GACGGCCTTC TCTGACTTCC TGATGGGCAG CTCCAAGGAC TTAGCCAAGC 
     3121 ACATCCGAGT AGTGTGTGAC GGAATGGACC TAACCCCCAA GATTCAGGAC CTGAAACCGC 
     3181 AGTGCATCGT CTTTCTGAAT ATCCCCAGGT ACTGTGCAGG CACCATGCCC TGGGGCCACC 
     3241 CCGGGGAGCA CCATGACTTC GAGCCCCAGA GGCATGATGA TGGCTACCTG GAGGTCATCG 
     3301 GCTTCACCAT GACATCCTTG GCAGCACTGC AGGTGGGTGG GCACGGCGAG CGATTGACGC 
     3361 AGTGCCGAGA AGTGCTGCTC ACCACTGCCA AGGCCATCCC TGTGCAGGTG GACGGTGAGC 
     3421 CCTGCAAGCT TTCAGCATCA CGGATTCGAA TTGCCCTGCG CAACCAGGCC ACTATGGTGC 
     3481 AGAAGGCCAA GCGCCGGAGT ACTGCCCCAC TGCACAGCGA CCAGCAGCCA GTTCCTGAGC 
     3541 AGCTGAGGAT CCAGGTGAGC AGGGTCAGTA TGCACGACTA CGAGGCTCTA CATTATGACA 
     3601 AGGAGCAGCT CAAGGAAGCC TCTGTGCCTC TGGGCACTGT GGTGGTCCCC GGAGACAGTG 
     3661 ACCTGGAGCT CTGCCGCGCC CACATTGAGA GACTGCAGCG GGAGCCCGAT GGGGCTGGAG 
     3721 CCAAGTCCCC GATGTGCCAC CAACTCTCCT CCAAGTGGTG TTTCCTGGAT GCCACCACTG 
     3781 CCAGCCGCTT CTACAGGATC GACAGAGCCC AGGAGCACCT CAACTATGTG ACGGAGATTG 
     3841 CCCAGGACGA GATTTACATC CTAGACCCAG AGCTTCTGGG AGCATCAGCC CGGCCTGACC 
     3901 TCCCCACCCC TACCTCCCCA CTCCCTGCTT CCCCCTGCTC CCCTACACCC GGGTCAATGC 
     3961 AGGGGGACAC TGCACTGCCC CAAGGTGAAG AACTGATTGA AGCAGCCAAG AGGAATGACT 
     4021 GCTGCAAGCT CCAGGAGCTA CACCGAGCAG GAGGTGACCT CATGCACCGT GACCAGAAGA 
     4081 GCCGCACGCT CTTGCACCAC GCGGTCAGCA CTGGCAGTAA GGAAGTGGTC CGCTATCTGC 
     4141 TGGATCATGC ACCTCCAGAG ATCCTTGATG CTGTGGAGGA GAATGGGGAG ACCTGTCTAC 
     4201 ACCAGGCAGC CGCCCTGGGT CAGCGCACCA TCTGCCACTA CATTGTGGAA GCCGGGGCCT 
     4261 CCCTCATGAA GACAGATCTG CAGGGCGACA CTCCCCGGCA GCGCGCTGAG AAGGCTCAAG 
     4321 ACACAGAGCT CGCTGCCTAC CTGGAGAACA GACAGCATTA CCAGATGATC CAGCGTGAGG 
     4381 ACCAGGAGAC AGCTGTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4441 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6061 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6121 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6181 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6241 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6301 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6361 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6421 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6481 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6541 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6601 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6661 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6721 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6781 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6841 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6901 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6961 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7021 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7081 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7141 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7201 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7261 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7321 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7381 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7681 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7741 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7801 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7861 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7921 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7981 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8041 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8101 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8161 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8221 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//