LOCUS       MR220742                8586 bp    DNA     circular  SYN 
DEFINITION  Phka2 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2), transcript variant 1
ACCESSION   NM_172783
VERSION     NM_172783.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="Phka2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGAGCAGG AGCAATTCTG GGGTCCGCCT GGACGGGTAC GCGAGGCTGG 
     1081 TGCAGCAAAC CATCCTGTGT TACCAGAACC CAGTCACTGG GCTGTTATCA GCCAGCCATG 
     1141 ACCAGAAGGA TGCCTGGGTA CGGGACAATA TCTATAGCAT ATTGGCTGTT TGGGGCCTGG 
     1201 GCATGGCCTA CCGCAAGAAT GCTGACCGTG ATGAGGATAA AGCCAAGGCC TATGAGTTGG 
     1261 AACAGAACGT AGTGAAGCTG ATGCGAGGTC TTCTCCAGTG TATGATGAGG CAGGTGGACA 
     1321 AAGTGGAGAA GTTCAAGCAC ACTCAGAGTA CCAAGGACAG TCTACATGCC AAGTACAACA 
     1381 CTGCCACATG TAGCACTGTG GTGGGTGATG ACCAGTGGGG CCACCTCCAG GTGGATGCCA 
     1441 CCTCTCTCTT CCTCCTGTTC CTGGCCCAGA TGACAGCCTC AGGTTTGCGT ATTATTTTTA 
     1501 CCCTTGACGA AGTGGCCTTC ATACAGAATC TTGTCTTCTA CATAGAAGCT GCATATAAAG 
     1561 TTGCTGATTA TGGGATGTGG GAGCGTGGAG ATAAAACCAA TCAGGGCATT CCGGAACTGA 
     1621 ATGCAAGCTC GGTGGGAGTG GCCAAGGCAG CACTTGAGGC CATTGATGAA CTAGATCTTT 
     1681 TTGGAGCCCA TGGAGGACGC AAATCGGTGA TCCACGTCCT ACCTGATGAA GTTGAGCACT 
     1741 GCCAGTCAAT TCTTTTCTCC ATGTTGCCAA GAGCATCAAC ATCTAAAGAA ATCGATGCTG 
     1801 GACTTCTTTC TATTATTTCT TTCCCGGCCT TTGCAGTAGA AGATGTGAAC CTTGTGAATG 
     1861 TGACCAAAAA TGAAATTATT TCCAAGCTTC AGGGGCGGTA TGGATGCTGT CGCTTCCTTC 
     1921 GAGATGGCTA TAAAACCCCA AGAGAGGATC CACACCGATT GCATTATGAC CCTGCCGAAC 
     1981 TTAAGCTCTT TGAAAACATT GAATGTGAAT GGCCTGTGTT CTGGACTTAT TTAATCATAG 
     2041 ATGGAATCTT CAATGGTGAT GCTGTTCAGG TCCAAGAATA CCGAGAAGCC TTGGAGGGAA 
     2101 TATTAATCAG AGGCAAAGAT GGGATCCACT TGGTGCCAGA ACTCTATGCC ATCCCACCAG 
     2161 ACAAGGTGGA TGAAGAGTAT AAGAATCCAC ACACAGTAGA CCGAGTTCCA CTGGGAAAGC 
     2221 TGCCCCATCT TTGGGGACAG TCCTTGTACA TCCTCAGCTC ACTGCTAGCA GAGGGATTCC 
     2281 TTGCCACGGG TGAAATCGAT CCCTTAAATA GAAGATTTTC TACTTCAGTC AAACCTGATG 
     2341 TTGTAGTGCA AGTTGCTGTT TTGGCAGAAA ACAGTCACAT TAAGGGGCTG TTGAAGGAGC 
     2401 ATGGAATGAC TGTCCAGAGC ATTGCTGATG TGCATCCGAT TCGAGTCCAG CCAGGCCGAA 
     2461 TTCTTAGTCA CATATATGCC AAACTTGGAC GAAATAAGAA TATGAAATTG AGTGGTCGAC 
     2521 CGTATCGGCA CATTGGTGTC CTTGGCACCT CTAAACTCTA TGTGATTAGG AATCACATCT 
     2581 TCACTTTTAC ACCCCAGTTC ACTGACCAGC ATCACTTCTA CCTGGCCCTG GACAATGAGA 
     2641 TGATTGTGGA GATGCTGAGG ATCGAACTTG CCTATCTGTG CACCTGCTGG CGGATGACCG 
     2701 GCCGACCCAC TCTCACCTTC CCTGTCACGC ATACCATGCT CACAAATGAT GGATCAGACA 
     2761 TTCATCCTGC AGTTCTTTCT ACAATTAGAA AACTAGAAGA TGGCTATTTT GGAGGTGCTA 
     2821 GAGTAAAACT AGGAAATCTG GCAGAGTTCC TTACTACCTC ATTCTACACA CACCTGACCT 
     2881 TCCTGGATCC AGACTGCGAT GAGAAGTTGT TTGGTGACAT CACTGATAGG AGCTTTAGTC 
     2941 CTGACAGTGA GCCAGACCTG GGAGGATACC TGGAAGACAG CAGTCCTCAA GAAAGCCAAG 
     3001 ATGAACTTGA CCAGTATATC AGCCACCTTC TTCAAAGCAC ATCCTTGAAG TGTTACCTGC 
     3061 CCCCTCTTTG TAAGAAGTCA GAAGACAGCC ATTTTTTCAG TGCTATTCAC TCCACTCGGG 
     3121 ACATACTTTC TGTGATGGCC AAAGCGAAGG GTTTGGAAAC TACATTTTTT CCCATGATTT 
     3181 TGCCAACTAA AGTTCTAAGT GGACACCGTA AGTCACTGAA TCTTGTTGAC TCCCCTCAGC 
     3241 CACTCCTAAA GACGACTCCT GAATATGACT ACCAGTGGCC CAGAGACGAC CATGATGAAG 
     3301 TGGACTGTGA GAAGCTAGTT GGGCAACTGA AAGACTGCTC AAACCTACAG GACCAAGCAG 
     3361 ACATTCTGTA CATTCTTTAT GTAATGAAGG GTCCCCGCTG GGATACCAAT TTGTTTGGGC 
     3421 AGCATGGAGT CACTGTTCAC AGTCTTCTCA GCGAGCTCTA TGGAAAAGCT GGCCTAAACC 
     3481 AAGAATGGAG TTTGATTCGC TACATTTCAG GCCTGCTCAG GAAGAAAGTG GAGGTCCTGG 
     3541 CTGAGGCCTG TGCAGATCTG CTGTCCCACC AGAAGCAGCT TACAGTAGGA CTGCCCCCTG 
     3601 AGCCCCGGGA GAAGACCATA TCTACGCCTC TACCCCCAGA GGAGCTCACA GAACTCATAT 
     3661 ATGAAGCCAG TGGACAGGAC ATCAGCATTG CTGTCCTCAC ACAGGAGATC GTGGTTTACC 
     3721 TGGCCATGTA TGTCCGGGCC CAGCCTAGCC TCTTTGCAGA GATGCTCAGA CTCCGGATTG 
     3781 GATTGATCAT CCAGGTGATG GCCACAGAGC TGGCTCGGAG CCTGAACTGC TCAGGAAAAG 
     3841 AAGCTTCGGA GAGCTTGATG AACCTCAGCC CTTTCGACAT GAAGAGCCTT CTGCATCATA 
     3901 TCCTGAGTGG GAAGGAGTTC GGTGTGGAGA GAAGCGTGCG CCCAATACAC TCCTCCATGT 
     3961 CCAGCCCTGC CATCTCCATC CATGAGGTGG GTCATACTGG AGCCACCAAA ACTGAGCGGA 
     4021 GTGGCATCAC CAGACTGAGG AGTGAGATGA AACAGATGAA TAGGCGAGCT AGTGCTGATG 
     4081 AGCAGTTCTT TCCTTTGGGC CAGACCATGT CCAACAGTTT GCATTCCATC AAGTCTGTGA 
     4141 GGTCCAGCAC CCCATCCTCC CCGACAGGCA CATCATCTAC AGACTCTGGA GGACAGCACC 
     4201 TGGGCTGGGG AGAACAGCAG GGCCAGTGGC TGCGCCGGAG AAGGTTGGAT GGGGCCATTA 
     4261 ACAGGGTCCC CGTGGGATTC TACCAGAAAG TGTGGAAGAT CCTTCAGAAG TGCCATGGCC 
     4321 TGTCCATCGA TGGTTACGTC CTCCCATCCT CGACAACCCA AGAGATGACC CCTTGTGAGA 
     4381 TCAAGTTTGC CGTCCATGTG GAGTCAGTGC TCAACCGTGT GTCCCAGCCT GAGTATCGGC 
     4441 AGCTACTGGT AGAAGCCATC ATGGTACTGA CTCTGCTCTC AGATACCGAA ATGGACAGTA 
     4501 TCGGGGGCAT CATCCATGTG GACCAGATAG TGCAGCTGGC CAATCAGCTG TTCCTGCAGG 
     4561 ACCAGGTGTC ATTTGGAACC ACAGACATCC TGGAAAAAGA CCAAGCCACA GGAATTTGCC 
     4621 ACCTCTTTTA TGACAGTGCT CCTAGTGGGG CTTATGGTAC AATGACCTAC CTAACAAAAG 
     4681 CGGTCGCTTC TCATTTGCAG GAACTGCTGC CCAGTTCAGG CTGCCAGATG CAGACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//