LOCUS       MR220718                6807 bp    DNA     circular  SYN 
DEFINITION  Kif12 (Myc-DDK-tagged) - Mouse kinesin family member 12 (Kif12)
ACCESSION   NM_010616
VERSION     NM_010616.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2964..3055 
                     /label="Myc-DDK"  
     misc_feature    3085..3670 
                     /label="PolyA signal"  
     misc_feature    3142..3161 
                     /label="Restriction Enzyme"  
     misc_feature    3946..4618 
                     /label="ColE1"  
     misc_feature    5048..5842 
                     /label="Kan/Neo"  
     misc_feature    5877..6179 
                     /label="SV40 ori"  
     misc_feature    6265..6742 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2946..2952 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2946 
                     /label="Kif12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAACGT GGGTCTCCCG ATGGAGACCC AGCACGGAAC CTGGAGCAAG 
     1081 GGCCAGAAGG ATCAGAAACT CCCATTCAAG TGGTGCTCAG GGTGCGTCCC ATGAGCACAG 
     1141 TGGAGCTGCG TCGAGGGGAG CAGAGTGCAT TGCACTGTTC GGGGACCAGG ACTCTGCAGG 
     1201 TGAGCCCCGA TGTGGCGTTC CGCTTTGGCG CCGTACTGGA CGGGGCGCGC ACGCAGGAGG 
     1261 ACGTGTTCAG AGCTTGCGGT GTGAAGCGCC TGGGTGAGCT GGCGCTGCGC GGTTTCTCCT 
     1321 GCACGGTCTT CACGTTTGGC CAGACTGGTT CTGGGAAGAC CTACACTCTG ACTGGACCTC 
     1381 CTCCGCAGGG GGAAGGGGTG CCCGTCCCCC CAAGCCTGGC TGGCATCATG CAGAGGACCT 
     1441 TCACCTGGCT ATTAGATCGC GTGCAGCACC TGGATTCCCC GGTCACCCTC CGAGCTTCCT 
     1501 ACCTGGAGAT CTACAATGAG CAGGTTTGGG ACTTGCTGAG TCTGGGGTCT CCACGGCCTC 
     1561 TGCCTGTTAG ATGGACCAAG GCACGAGGCT TCTATGTGGA ACAGCTTCGG GTGGTGGAGT 
     1621 TTGGCAGTCT GGAGGCTTTG ATGGAGCTCC TGCAGATGGG TCTCAGCCGT CGTCGGAGCT 
     1681 CCTCACATAC CTTGAACCAG GCCTCCAGCA GAAGCCATGC CCTGCTTACA CTCCACATCA 
     1741 GCCGCCCAAC TTCTCAGCAG GTACCTCCTG TGGACCTTGG GGAGCCCCCT GTTGGAGGGA 
     1801 AGCTGTGCTT TGTGGATCTT GCAGGCAGTG AGAAAGTGGC AGCCACAGGA TCCCAAGGGC 
     1861 AGCTGATGCT TGAGGCCAAT AGCATCAACC GCAGCCTATT GGCATTGGGC CACTGCATCT 
     1921 CCCTGCTGTT GGACCCACAG AGGAAGCAGA ACCACATTCC CTTCCGGGAC AGCAAGCTCA 
     1981 CCAAGTTGCT GGCAGACTCA CTTGGGGGCC GTGGGGTCAC ACTCATGGTT GCCTGCGTGT 
     2041 CCCCTTCAGC GCAGTGCCTT CCTGAGACTC TCAGCACTCT GCGATATGCA AGCCGAGCTC 
     2101 AGCGGATCAC CACCCGGCCA CAGGGTCCCA AGTCCCCTGG AGTAAAGCCA CCCCAGCAAG 
     2161 TAGAGAACGA GTTGCTAAGG CTCCAAGAGG AGAATCGTCA CCTGCGGTTC CAGCTGGATC 
     2221 AGATGCACAC CACGGCACCA GGGGCCCATG GAGCCCGGAT GGCCTGGGCC CAGAGAAACC 
     2281 TCTATGGGAT GCTGCAGGAG TTTATGCTGG AGAACGAGAG GCTCAGGAAG GAAATGAGAC 
     2341 AGCTGAGGAG TAGCCGGGAC CTGGCCCGGG CAGAGCAGCG TGTCCTGGCC CAACAGGTCC 
     2401 ATGATCTGGA GAGGCGCCTC CTTTCGGCTT GTCCCCTTCC CCAGCAGGGC TCTACCCCTG 
     2461 TGTGTCCCTG CAGGATGGTT CCAGCTGCCT CCTGCCATGC ACTGCCACCC CTCTGTTACT 
     2521 GCCACCACTT CTGTCCTCTG TGCCGAGTGC CCTTGGCCCA CTGGACCTGC CCACGGAGAG 
     2581 AGTGCCACAT GCCACAGGTG CTGGAGCCCG AGGCCCCAGG TCACATATCT CAGTCTGTGT 
     2641 GGCCCCCACC CTGGGCACCT CCACCAAGCC CAGGCTCTGC CAAGCCCCCA CGAGAGAGGA 
     2701 GCCAGAGTGA CTGGACCCAG ACACGAGTCC TGGCAGAGAT GCTGATGGGA GAAGAAGTGG 
     2761 TACCCTCCGC ACCACCCCTG TCTGCAGGGC CCTCGAACAT GCCATATGGG CTAAGAGGAG 
     2821 GATCTGGGAT TCCAAACCTG ACCCCGAGGC TGGAAACCCT CACACAGCAG ATCAACAGCT 
     2881 CCCTGCACCT CAGCCAGAGA CAACCACAGC CTAGCGAGGA CACACAGAGC CCTGGCCAAG 
     2941 GCCTCTCTTC TTGTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3001 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3061 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3121 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3181 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3241 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3301 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3361 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3421 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3481 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3541 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3601 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3661 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3721 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3781 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3841 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     3901 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     3961 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4021 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4081 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4141 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4201 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4261 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4321 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4381 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4441 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4501 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4561 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4621 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4681 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4741 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4801 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4861 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     4921 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     4981 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5041 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5101 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5161 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5221 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5281 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5341 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5401 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5461 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5521 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5581 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5641 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5701 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5761 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5821 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     5881 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     5941 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6001 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6061 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6121 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6181 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6241 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6301 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6361 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6421 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6481 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6541 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6601 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6661 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6721 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6781 ATTTAACAAA AATTTAACGC GAATTTT
//