LOCUS       MR220692                7671 bp    DNA     circular  SYN 
DEFINITION  Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o)
ACCESSION   NM_173755
VERSION     NM_173755.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3828..3919 
                     /label="Myc-DDK"  
     misc_feature    3949..4534 
                     /label="PolyA signal"  
     misc_feature    4006..4025 
                     /label="Restriction Enzyme"  
     misc_feature    4810..5482 
                     /label="ColE1"  
     misc_feature    5912..6706 
                     /label="Kan/Neo"  
     misc_feature    6741..7043 
                     /label="SV40 ori"  
     misc_feature    7129..7606 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3810..3816 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3810 
                     /label="Ube2o"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTCCGTC CGAGCCGCCA ACCGGAGACC CAGGCGAGTG TCGCGGCCGC 
     1081 GCCCGGTGCA ACAGCAGCAG CAGCAGCCCC CGCAGCAGCC GCCGCCGCAG CCGCCTCAGC 
     1141 AACAGCCGCC GCCGCAGCCG CCCCAGCAGC CGCCGCAGCA GCAGCCCCCG CCGCCGCCGC 
     1201 AGCAGCAGCC CCCGCCGCCG CCCCCGCCGC CTCCGCCGCC GCCTCAGGAT CGGAACAACG 
     1261 CCGGCGAGAG GGATGATGTT CCTGCAGATA TGGTTGCAGA AGAATCAGGT CCTGGTGCAC 
     1321 AAAATAGTCC ATACCAACTT CGCAGAAAAA CTCTTTTGCC AAAAAGAACA GCGTGTCCTA 
     1381 CAAAGAGCAG TATGGAGGGT GCCTCTACTT CGACTACAGA AAACTTTGGT CATCGAGCAA 
     1441 AGCGTGCAAG AGTGTCTGGA AAGTCACAAG ATCTTTCAGC AGCACCCGCT GAACAGTATC 
     1501 TTCAGGAGAA GCTGCCAGAT GAAGTAGTTT TAAAAATATT TTCTTATTTG CTGGAACAAG 
     1561 ACCTTTGTAG AGCTGCTTGC GTGTGTAAAC GCTTCAGTGA GCTTGCTAAT GATCCAATTT 
     1621 TGTGGAAACG ATTATATATG GAAGTATTTG AATATACCCG GCCGATGATG CATCCTGAAC 
     1681 CTGGTAAATT CTACCAGATT AATCCAGAAG AATATGAACA TCCAAATCCA TGGAAAGAAA 
     1741 GTTTTCAACA GTTGTATAAA GGTGCGCATG TAAAGCCAGG ATTTGCGGAA CATTTCTATA 
     1801 GTAATCCTGC AAGATACAAA GGAAGAGAAA ACATGTTGTA TTATGATACT ATTGAAGATG 
     1861 CCCTTGGAGG AGTACAAGAA GCACATTTTG ATGGGCTTAT CTTTGTTCAT TCTGGAATAT 
     1921 ATACTGATGA ATGGATATAT ATTGAATCTC CAATTACTAT GATTGGTGCA GCACCTGGCA 
     1981 AGGTTGCAGA CAAGGTCATC ATTGAGAACA CTAGAGATTC GACCTTCGTG TTCATGGAAG 
     2041 GCTCTGAAGA TGCTTATGTT GGATACATGA CGATAAGATT CAACCCTGAT GACAAATCTG 
     2101 CTCAGCATCA CAACGCACAC CACTGCTTAG AGATCACAGT CAACTGCAGC CCTATTATTG 
     2161 ACCACTGTAT AATCCGCAGC ACATGCACAG TTGGTTCTGC AGTGTGTGTC AGTGGCCAGG 
     2221 GGGCGTGTCC CACTATCAAG CATTGTAACA TCAGTGACTG TGAAAACGTC GGACTTTATA 
     2281 TAACAGATCA TGCACAGGGA ATATATGAAG ATAATGAAAT TTCTAATAAT GCGTTAGCTG 
     2341 GGATTTGGGT TAAAAATCAT GGAAATCCAA TTATTAGACG GAATCACATT CATCATGGAC 
     2401 GTGATGTTGG TGTGTTCACA TTTGATCATG GCATGGGCTA CTTTGAAAGT TGCAACATAC 
     2461 ACAGAAACAG GATAGCAGGC TTTGAGGTAA AAGCCTATGC CAATCCCACA GTGGTTCGAT 
     2521 GTGAGATTCA CCACGGGCAG ACTGGAGGAA TATACGTCCA CGAGAAAGGA AGAGGACAGT 
     2581 TCATAGAGAA CAAGATCTAT GCAAACAATT TCGCAGGCGT GTGGATTACC TCAAATAGTG 
     2641 ACCCAACAAT AAGGGGGAAT TCTATATTTA ATGGAAATCA AGGAGGAGTT TACATCTTTG 
     2701 GTGACGGACG AGGCCTTATA GAAGGAAATG ACATTTATGG TAATGCGTTA GCAGGAATTC 
     2761 AGATCAGGAC AAACAGTTGT CCTATTGTTC GACATAACAA AATTCATGAT GGACAGCATG 
     2821 GTGGGATTTA TGTGCATGAA AAGGGGCAAG GAGTAATAGA AGAGAATGAA GTTTACAGCA 
     2881 ACACTCTGGC TGGCGTCTGG GTGACAACTG GCAGCACACC AGTTCTAAGA AGAAACCGGA 
     2941 TACACAGTGG CAAGCAGGTT GGTGTTTATT TCTATGACAA TGGACATGGT GTTCTAGAAG 
     3001 ATAACGACAT CTACAATCAC ATGTATTCAG GGGTTCAGAT AAGAACTGGA AGCAACCCCA 
     3061 AAATTAGACG CAACAAAATT TGGGGAGGAC AGAATGGTGG AATTCTAGTT TATAATTCTG 
     3121 GTCTAGGCTG TATAGAAGAC AATGAAATAT TTGACAACGC GATGGCTGGA GTCTGGATTA 
     3181 AAACAGATAG TAATCCTACG CTAAGAAGAA ATAAAATCCA TGATGGAAGA GATGGTGGCA 
     3241 TCTGTATATT TAATGGGGGT CGAGGTCTCC TTGAAGAAAA TGATATTTTC AGGAACGCTC 
     3301 AAGCAGGTGT CCTCATCAGC ACTAACAGTC ATCCAGTATT AAGGAAAAAC AGAATATTTG 
     3361 ATGGATTTGC TGCAGGTATT GAAATTACAA ATCATGCAAC TGCAACACTC GAAGGCAATC 
     3421 AGATCTTTAA CAACCGATTT GGAGGCTTAT TTTTAGCATC TGGTGTTAAT GTGACAATGA 
     3481 AAGATAACAA AATAATGAAC AATCAAGATG CCATAGAAAA GGCTGTTAGT AGAGGACAAT 
     3541 GTCTATATAA AATATCAAGT TATACCAGCT ACCCCATGCA TGACTTCTAC AGATGTCATA 
     3601 CTTGTAACAC CACAGATCGA AATGCCATAT GTGTGAACTG CATTAAGAAG TGCCATCAGG 
     3661 GACATGATGT AGAGTTCATT AGACATGATA GGTTTTTCTG TGACTGTGGT GCTGGAACGC 
     3721 TGTCTAATCC TTGTACCTTA GCTGGCGAGC CTACACACGA CACAGACACA CTCTATGACT 
     3781 CTGCCCCACC TATAGAATCT AATACGTTGC AGCACAACAC GCGTACGCGG CCGCTCGAGC 
     3841 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3901 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3961 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4021 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4081 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4141 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4201 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4261 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4321 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4381 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4441 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4501 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4561 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4621 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4681 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4741 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4801 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4861 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4921 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4981 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5041 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5101 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5161 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5221 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5281 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5341 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5401 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5461 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5521 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5581 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5641 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5701 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5761 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5821 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5881 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5941 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6001 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6061 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6121 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6181 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6241 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6301 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6361 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6421 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6481 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6541 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6601 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6661 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6721 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6781 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6841 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6901 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6961 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7021 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7081 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7141 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7201 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7261 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7321 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7381 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7441 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7501 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7561 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7621 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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