LOCUS       MR220584                7467 bp    DNA     circular  SYN 
DEFINITION  Agbl2 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 2 (Agbl2)
ACCESSION   NM_178755
VERSION     NM_178755.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3624..3715 
                     /label="Myc-DDK"  
     misc_feature    3745..4330 
                     /label="PolyA signal"  
     misc_feature    3802..3821 
                     /label="Restriction Enzyme"  
     misc_feature    4606..5278 
                     /label="ColE1"  
     misc_feature    5708..6502 
                     /label="Kan/Neo"  
     misc_feature    6537..6839 
                     /label="SV40 ori"  
     misc_feature    6925..7402 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3606..3612 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3606 
                     /label="Agbl2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATGTCCTG CTTGAGATGG CTTTTCTTTC ACAGACTCTT CCTGATCCCT 
     1081 ATGAGGACTT TATTCACCAT CACCTCCAGT ATTATGGATA TTTCAAAGCT CAGAAAAGCA 
     1141 GCCTGCCAAA CTCAGGCACT CATCAGCGCG TTTGGAGGAA CAGCCCTCGG TACATGTTGA 
     1201 ATGGCTCTTT TGGAGAAAGA GATGATTTCA TCTCGGACAG TTTGGAAAAA GAGATGCTTC 
     1261 TGTGGCCTAC CTGTTTATCT TCAACTGGGC ATGCTCACAT AGACGCTGTA AACAGAGACT 
     1321 CACTTCTGCT GAGCTCGCCG CTCCTGAGGA CCAGGCAGCT GATTTTTGAT GAGCTAGATG 
     1381 AAGCAAGCCC ACGGCTTCGA GAACCCCGAG AACTCTTCTC ATGTTTCTCT AGCAGAGGGC 
     1441 CGCTGCAGGC TCCAAGATGG CCAATCGAAT GTGAGGTCAT CAAGGAAAAC ATTCATCACA 
     1501 TTGAGTGGGT TCCACATCAA CCAGAATATT TCTACCAACC TACAGGAAGC GAGAAGGTGC 
     1561 CAGAGATTGT GGGAGAGGAG CAAGGTACAG TTGTGTACCA GCTAGATTCA GTGCCTGCAG 
     1621 AAGGCACCTA TTTCACTAGT TCCAGAATAG GAGGCAAACG AGGGACCATC AAGGAACTTG 
     1681 CTGTCACACT GCAAGGGCCG GACGACAATA CTCTATTGTT TGAGTCAAGG TTTGAGAGCG 
     1741 GGAATCTGCA GAAAGCCGTC AGAGTAGGTA TCTATGAGTA CGAGCTCACC TTGCGGACTG 
     1801 ACCTCTACAC GGACAAACAC ACTCAGTGGT TTTACTTCAG AGTTCAGAAC ACCAGAAAAG 
     1861 ACGCTACCTA TCGCTTCACC ATCGTCAACT TGCTGAAACC CAAGAGCCTT TACGCAGTAG 
     1921 GCATGAAGCC TCTCATGTAC TCTCAGCTGG ATGCCACCAT CTACAACATC GGCTGGAGGA 
     1981 GAGAGGGCCG TGAAATCAAG TACTACAAGA ACAATGTGGA CGACGGCCAG CAGCCCTTAT 
     2041 ACTGCCTCAC CTGGACCACT CAGTTTCCTC ACGACCAGGA CACTTGCTTC TTCGCACACT 
     2101 TTTACCCATA CACTTATACT GACTTGCAAT GCTATCTCTT GTCAGTAGCA AACAACCCCA 
     2161 TCCAGTCTCA GTTCTGCAAG CTCCGAGCCC TATGCAGGAG CCTAGCAGGG AACACTGTCT 
     2221 ACCTGCTGAC CATCACCAAC CCATCCAGGA CCCCTCAAGA GGCAGCCGCA AAGAAAGCTG 
     2281 TGGTCTTGAG CGCCCGAGTC CACCCTGGAG AGAGTAACAG CTCCTGGATC ATGAACGGCT 
     2341 TTTTGGACTT CATCCTTAGC AACTCTCCCG ATGCCCAGCT CCTCAGGGAT ATCTTTGTCT 
     2401 TCAAGGTGAT TCCCATGTTA AACCCAGATG GCGTGATAGT GGGGAACTAT CGGTGTTCGC 
     2461 TGGCTGGAAG GGACTTGAAC AGGCATTATA AAACCGTTCT TAAGGACTCC TTCCCTTGCA 
     2521 TTTGGTACAC CAAGAACATG ATCAAAAGGC TTCTGGAAGA AAGAGAGGTT CTCTTGTACT 
     2581 GTGATTTCCA TGGGCATAGC CGCAAGAACA ACATCTTCCT GTATGGCTGT CACAGCAACA 
     2641 ACCGCAAGCA CTGGCTCCAT GAGCGTGTCT TTCCTTTAAT GTTGAGCAAG AATGCACCAG 
     2701 ACAAGTTCTC TTTCGACAGC TGTAACTTTA AGGTCCAAAA ATGCAAAGAA GGAACGGGAA 
     2761 GAGTGGTGAT GTGGCGGATG GGCATCATCA ACAGCTACAC CATGGAGTCT ACCTTTGGCG 
     2821 GGTCTACCCT GGGTAGTAAA AGAGACACTC ACTTTACCAT TGAGGACCTG AAGTCCCTAG 
     2881 GGTATCATGT CTGTGACACT CTTCTGGATT TCTGTGATCC TGACCAAACC AAGTACACAC 
     2941 AGTGTCTACA AGAGCTTAAG GAGCTCCTAC AACAGGAAAT CAACAAAAAG TTGAATAACT 
     3001 TTGGACAAGA TATGGATTTA GAAGGAAACT GGAGTGACAT TCCTTTGTCT GACATTGAAT 
     3061 CCAGCACGAG TGGCTCGGAC AGCTCCCTCT CCGATGGCCC TCCCATTCGT CTACTGAACA 
     3121 TCGTTGCAGA TGAGCCAAAT CAGAAGACGG TGTTAAAGAA TCCAAAAAAG AAGAGACTTC 
     3181 AGACTAGAAA ACAGCGGAAT GAGCAGTACC AGAAGAGCTA CTTGATGCGG GAGTTAAAGT 
     3241 TAACAGAAAA CGCCCCAGGA AGGGCGAGAT TTGTCTCTAC TCTGCAAAAG CAGCCTACTT 
     3301 TTTTGAAAAG TCCAGAGAGT CCTAGCCCTT CAGTAAGGAG AAGTGAAAAC CCAAGGCTCA 
     3361 ATGAGACACA GTTAAGTGGA AGAGAGAAGG GCACCTCCCT GGATCCACCA CTGACCAGCC 
     3421 CTAAGAATAA AGAAAGAATT CAGAGTAAGA AGCCAGGCTT TACAGCATCA TGCTCTCCAA 
     3481 AGAGAAGCAC AAACTCCAGC CTAGGGCCAG CTCCAGATGT GAAGCCAAAC TGGTCTAAGA 
     3541 CTAGATACTC TGCCACAAGG AAGGACCATG CCACCATGGC CGTGTACCCA TCCTTGCATA 
     3601 TATACACATA CCCAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3661 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3721 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3781 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3841 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3901 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3961 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4021 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4081 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4141 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4201 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4261 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4321 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4381 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4441 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4501 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4561 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4621 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4681 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4741 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4801 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4861 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4921 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4981 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5041 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5101 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5161 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5221 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5281 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5341 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5401 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5461 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5521 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5581 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5641 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5701 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5761 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5821 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5881 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5941 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6001 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6061 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6121 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6181 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6241 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6301 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6361 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6421 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6481 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6541 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6601 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6661 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6721 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6781 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6841 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6901 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6961 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7021 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7081 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7141 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7201 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7261 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7321 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7381 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7441 ATTTAACAAA AATTTAACGC GAATTTT
//