LOCUS       MR220516                8100 bp    DNA     circular  SYN 
DEFINITION  Plch1 (Myc-DDK-tagged) - Mouse phospholipase C, eta 1 (Plch1), transcript variant 2
ACCESSION   NM_001177733
VERSION     NM_001177733.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4257..4348 
                     /label="Myc-DDK"  
     misc_feature    4378..4963 
                     /label="PolyA signal"  
     misc_feature    4435..4454 
                     /label="Restriction Enzyme"  
     misc_feature    5239..5911 
                     /label="ColE1"  
     misc_feature    6341..7135 
                     /label="Kan/Neo"  
     misc_feature    7170..7472 
                     /label="SV40 ori"  
     misc_feature    7558..8035 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4239..4245 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4239 
                     /label="Plch1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGACCTT GAAGTGTATA AAAACTTAAG TCCAGAAAAA GTTGAAAGAT 
     1081 GCATGAGTGT AATGCAGTCC GGGACACAGA TGATCAAACT GAAGCGTGGC ACCAAAGGGC 
     1141 TCGTTCGCCT CTTTTACCTG GATGAGCACC GGACCCGCCT CCGATGGCGA CCCTCTCGGA 
     1201 AGAGTGAGAA GGCAAAAATA CTTATTGACT CCATCTACAA AGTCACCGAG GGCAGGCAGT 
     1261 CTGAAATATT CCACAGACAG GCTGAGGGGA ACTTTGACCC TAGCTGCTGC TTTACCATCT 
     1321 ACCATGGCAA CCACATGGAG TCTCTGGACC TCATTACCTC CAACCCAGAG GAGGCACGCA 
     1381 CCTGGATCAC GGGCCTCAAG TATCTGATGG CTGGCATCAG TGATGAAGAC TCCCTTGCCA 
     1441 AGAGGCAGAG GACCCATGAC CAATGGGTGA AGCAGACCTT TGAGGAAGCT GATAAGAACG 
     1501 GTGATGGCTT ATTGAATATT GAAGAGATTC ACCAGCTGAT GCATAAACTA AATGTCAATC 
     1561 TGCCCCGCAG AAAAGTCAGG CAAATGTTTC AGGAAGCAGA TACAGATGAG AATCAGGGAA 
     1621 CCTTGACATT TGAAGAGTTC TGCGTTTTTT ACAAAATGAT GTCATTGAGA CGAGACCTCT 
     1681 ATTTGCTGCT CTTGAGCTAC AGTGACAAGA AAGACCACCT GACTGTGGAG GAGCTGGCTC 
     1741 AGTTTTTGAA AGTGGAACAA AAGATGAGTA ATGTGACACT GGACTATTGT CTTGACATCA 
     1801 TAATGAAGTT TGAAGTTTCT GAAGAAAACA AAGTGAAAAA CGTCCTTGGT ATAGAAGGCT 
     1861 TCACGAACTT CATGCGTAGC CCTGCCTGTG ACGTATTTAA CCCGTTGCAC CATGAAGTGT 
     1921 ACCAAGACAT GGATCAGCCC CTGTGCAACT ACTACATTGC TTCATCTCAC AACACATACC 
     1981 TGACTGGGGA TCAGCTCCTT TCTCAATCTA AAGTGGATAT GTATGCACGG GTGCTACAAG 
     2041 AGGGTTGTAG ATGCGTGGAA GTTGACTGTT GGGATGGCCC AGATGGAGAG CCAGTGGTCC 
     2101 ACCATGGTTA TACTCTCACT TCAAAAATTC TCTTCAGAGA TGTTGTGGAG ACCATCAACA 
     2161 AGCATGCATT CGTGAAGAAT GAGTTCCCGG TTATCCTGTC CATTGAGAAC CACTGCAGCA 
     2221 TTCAGCAGCA GAGGAAGATT GCTCAGTACC TGAAAGGTAT ATTGCAGGAC AAACTGGACC 
     2281 TGTCTTCTGT AGACACCGGA GAGTGCAGGC AGCTTCCAAG CCCTCAGAGT CTGAAAGGCA 
     2341 AAATCCTAGT GAAGGGCAAG AAGTTGCCGT ATCACCTTGG GGATGATGCA GAGGAAGGAG 
     2401 AAGTGTCCGA TGAGGACAGT GCAGATGAGA TTGAAGATGA GTGCAAGTTC AAGCTGCATT 
     2461 ACAGTAACGG GACCACTGAG CACCAGGTAG AATCTTTCAT ACGGAAAAAG CTGGAGTCAC 
     2521 TGTTGAAGGA GTCTCAGATT CGAGACAAAG AAGATCCAGA CAGTTTCACA GTGAGGGCGT 
     2581 TACTCAAGGC TACACATGAA GGCTTAAATG CACACCTGAA GCAGAACCTG GATGTAAAGG 
     2641 AAAGTGGAAA GAAGTCCCAT GGGCGATCCC TGATGGCCAA CTTTGGGAAA CACAAGCAGA 
     2701 AAGCTACGAA ATCACGTTCT AAATCCTATA GTACTGATGA TGAGGATGAT AGCCTTCAGA 
     2761 ATCCTGGCAA GGAGGGAGGC CAGCTGTACA GGCTGGGCCG CCGAAGGAGA ACCATGAAAC 
     2821 TCTGCAGAGA GCTCTCGGAC TTGGTGGTGT ACACAAACTC CGTGGCAGCC CAGGATATTG 
     2881 TTGATGATGG AACCACAGGG AATGTGTTGT CCTTCAGCGA AACAAGAGCC CATCAAGTGG 
     2941 TTCAGCAGAA GTCAGAGCAG TTCATGATCT ACAACCAAAA ACAGCTCACA AGGATTTACC 
     3001 CCTCTGCCTA CCGCATCGAT TCCAGTAACT TCAACCCACT GCCCTACTGG AATGCAGGCT 
     3061 GCCAGCTCGT GGCTCTGAAC TACCAGTCTG AAGGGCGAAT GATGCAGATC AATCGAGCAA 
     3121 AATTCAAGGC GAATGGCAAC TGTGGCTATA TTCTCAAGCC CCAGCAAATG TGCAAAGGTA 
     3181 CTTTCAACCC TTTCTCTGGT GACCCACTTC CTGCCAACCC CAAAAAACAG CTCATACTGA 
     3241 AAGTGATCAG TGGACAGCAA CTCCCCAAAC CTCCTGACTC TATGTTTGGA GACCGAGGCG 
     3301 AGATCATTGA CCCTTTCGTT GAAGTTGAAA TTATTGGGCT GCCAGTAGAC TGTTGCAAGG 
     3361 ACCAAACTCG TGTGGTGGAT GACAACGGAT TTAACCCTGT GTGGGAAGAA ACCCTAACTT 
     3421 TTACAGTTCA CATGCCAGAA ATAGCTTTGG TTCGGTTCCT TGTGTGGGAT CATGATCCTA 
     3481 TTGGAAGAGA CTTTGTCGGA CAAAGGACTG TGACCTTCAG TAGCCTCGTG CCCGGCTACC 
     3541 GGCATGTCTA CCTGGAAGGA CTCACAGAAG CATCTATTTT CGTTCACATA ACCATCAATG 
     3601 AGATCTTTGG AAAGTGGAGT CCTTTAATCC TCAACCCCAG TTATACCATA TTGCACTTTC 
     3661 TAGGAGCTAC AAAGAACAGA CAGCTCCAAG GCCTGAAGGG GCTGTTCAAT AAGAATCCCA 
     3721 GGCACGCTTC TTCAGAAAAC AACTCGCATT ATGTTCGGAA GCGATCGATT GGAGATAGGA 
     3781 TTCTGCGACG TACAGCTAGT GCTCCAGCCA AAGGCAGGAA AAAGAGCAAA GTGGGCTTCC 
     3841 AAGAAATGGT TGAGATAAAA GATTCTGTCT CTGAGGCTTC AAGAGATCAA GATGGTGTCC 
     3901 TGAGGAGGAC CACCCGGAGT CTGCAAGTAC GCCCTGTCTC TATGCCTGTT GACAAGAGCC 
     3961 TTCTGGGGGC TTTGTCGCTG CCCATATCTG AAGCAGCAAA AGACACGGAT GGAAAAGAAA 
     4021 ACTGTCTGGA TCTGAATAGG AAGCAGCGCA AGCAGGAGAC AAGGATGACA GAAGAAAGGG 
     4081 AGCCGCAACT AGAAAAGACC CACATTTTTC AAATTTCAAC AAAAAGTTAT CCTCCTCCTC 
     4141 CAGTGCGCTC CTCCACAAAG ATGCCAACCA AGGGCCAACT GCCAGTGTAT CAAACCCAGA 
     4201 ACAGTGTGGA GGACGAGGTG CAAAGAGTGA GAGGATCAAA CCAAATAACG CGTACGCGGC 
     4261 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4321 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4381 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4441 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4501 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4561 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4621 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4681 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4741 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4801 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4861 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4921 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4981 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5041 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5101 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5161 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5221 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5281 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5341 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5401 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5461 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5521 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5581 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5641 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5701 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5761 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5821 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5881 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5941 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6001 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6061 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6121 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6181 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6241 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6301 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6361 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6421 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6481 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6541 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6601 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6661 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6721 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6781 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6841 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6901 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6961 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7021 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7081 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7141 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7201 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7261 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7321 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7441 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7501 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7561 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7621 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7681 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7741 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7801 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7861 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7921 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7981 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8041 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//